公开(公告)号：KR1020090096890A

公开(公告)日：2009-09-15

申请号：KR1020080021970

申请日：2008-03-10

Applicant: 전남대학교산학협력단

Inventor： 김도만 ,  김영민 ,  김도원 ,  훵충리 ,  잉따우 ,  빙상브레통

IPC: C12N15/64 ,  C12N15/09 ,  C12N15/44

CPC classification number: C12N15/70 ,  C12N9/2402 ,  C12Q1/34 ,  C12Y302/01018 ,  G01N2500/04

Abstract: An E.coli expression vector of neuraminidase N1 of avian influenza (AI) is provided to quickly and massively produce AI virus neuraminidase N1 and select an inhibitor of AI virus neuraminidase N1 as a therapeutic agent. A vector for expressing neuraminidase N1 of avian influenza (AI) in E.coli comprises a neuraminidase N1 gene of avian influenza (AI) in multiple cloning site of pET-23d vector. A method for producing the neuraminidase N1 gene of avian influenza (AI) comprises: a step of transforming E.coli with the vector which expresses the neuraminidase N1; and a step of culturing the transformed E.coli.

Abstract translation: 提供禽流感（AI）神经氨酸酶N1的大肠杆菌表达载体，快速大量产生AI病毒神经氨酸酶N1，并选择AI病毒神经氨酸酶N1抑制剂作为治疗剂。 用于在大肠杆菌中表达禽流感（AI）的神经氨酸酶N1的载体包含在pET-23d载体的多克隆位点中禽流感（AI）的神经氨酸酶N1基因。 制备禽流感（AI）神经氨酸酶N1基因的方法包括：用表达神经氨酸酶N1的载体转化大肠杆菌的步骤; 以及培养转化的大肠杆菌的步骤。