公开(公告)号：KR101145689B1

公开(公告)日：2012-05-24

申请号：KR1020090122011

申请日：2009-12-09

Applicant: 전남대학교산학협력단

Inventor： 김정일 ,  김용민 ,  조규창 ,  한윤정

IPC: C12N15/82 ,  C12N15/29 ,  A01H1/00

Abstract: PURPOSE: A transgenic lawn grass with herbicide resistance and diseases resistance is provided to maintain stability and to enhance biological and abiotic stress resistance. CONSTITUTION: A transgenic lawn grass with herbicide resistance and diseases resistance is obtained by transforming with a vector containing PepEST1 and Bar genes. The vector is transformed to grass embryonic developing callus by agrobacterium. The callus is cultured in a callus-inducing medium containing 0.2-0.8 mg/L of kinetin. The herbicide contains glufosinate as an active ingredient. The grass is Bentgrass.

公开(公告)号：KR1020150026420A

公开(公告)日：2015-03-11

申请号：KR1020130105227

申请日：2013-09-03

Applicant: 전남대학교산학협력단

Inventor： 김정일 ,  황옥진 ,  김도순 ,  한윤정

IPC: A01H5/00 ,  A01H1/06 ,  C12N15/82

CPC classification number: A01H5/00 ,  A01H1/06 ,  C12N15/82

Abstract: The present invention provides a method for manufacturing a new variety of Miscanthus sinensis having herbicide resistance by a transformation technique of Miscanthus sinensis using Agrobacterium tumefaciens. The present invention relates to a manufacturing method of a transgenic Miscanthus sinensis plant; to a transgenic Miscanthus sinensis plant manufactured by the method; and to seeds of the transgenic Miscanthus sinensis plant, wherein the method comprises the steps of (a) sterilizing mature seeds of Miscanthus sinensis of which seed coats have been removed; (b) placing the seeds of Miscanthus sinensis which have been sterilized on a callus induction medium to induce an embryogenic callus of Miscanthus sinensis; (c) inoculating an inoculation medium with Agrobacterium tumefaciens comprising a plant expression vector into which a selectable marker and an exotic gene are inserted and with the embryogenic callus of Miscanthus sinensis which has been induced, followed by performing a cocultivation process in a cocultivation medium to transform the embryogenic callus of Miscanthus sinensis; (d) selecting plant cells which have been cocultivated in a selection medium to which an antibiotic or a herbicide is added; and (e) performing a redifferentiation process of Miscanthus sinensis cells which have been selected in a redifferentiation medium.

Abstract translation: 本发明提供一种通过使用根癌土壤杆菌通过中国芒草的转化技术制造具有除草剂抗性的新品种的芒草的方法。 本发明涉及一种转基因芒草植物的制造方法， 通过该方法制造的转基因芒草植物; 以及所述转基因芒草种子，其中所述方法包括以下步骤：（a）对已经除去种皮的芒草的成熟种子进行灭菌; （b）将已经灭菌的芒草的种子放置在愈伤组织诱导培养基上以诱导中国芒草的胚发生愈伤组织; （c）用包含植物表达载体的土壤杆菌接种培养基，所述植物表达载体插入其中选择标记和外来基因并与已经诱导的芒草的胚发生愈伤组织接触，随后在共培养培养基中进行共培养过程 转化中国芒草的胚性愈伤组织; （d）选择在添加了抗生素或除草剂的选择培养基中共培养的植物细胞; 和（e）进行在再分化培养基中选择的芒草细胞的再分化过程。

公开(公告)号：KR101555270B1

公开(公告)日：2015-09-25

申请号：KR1020130150551

申请日：2013-12-05

Applicant: 전남대학교산학협력단

Inventor： 김정일 ,  박문영 ,  신아영 ,  한윤정

IPC: C12N15/82 ,  C12N15/29 ,  C07K14/415 ,  A01H5/00

Abstract: 본발명은애기장대() 유래 At3g52740 단백질코딩유전자를포함하는재조합벡터를식물세포에형질전환하여식물체의바이오매스생산성을증가시키거나또는종자수확량을증가시키는방법, 상기 At3g52740 단백질코딩유전자를포함하는재조합벡터를식물세포에형질전환하여식물체의바이오매스생산성이증가되거나또는종자수확량이증가된형질전환식물체의제조방법, 상기방법에의해제조된형질전환식물체및 이의종자, 상기 At3g52740 단백질코딩유전자를유효성분으로포함하는, 식물체의바이오매스생산성을증가시키거나또는종자수확량을증가시키기위한조성물, 상기애기장대 At3g52740 단백질코딩유전자유래프로모터, 상기프로모터를포함하는재조합식물발현벡터및 상기재조합식물발현벡터로형질전환된식물세포에관한것이다.

公开(公告)号：KR1020110065140A

公开(公告)日：2011-06-15

申请号：KR1020090122011

申请日：2009-12-09

Applicant: 전남대학교산학협력단

Inventor： 김정일 ,  김용민 ,  조규창 ,  한윤정

IPC: C12N15/82 ,  C12N15/29 ,  A01H1/00

Abstract: PURPOSE: A transgenic lawn grass with herbicide resistance and diseases resistance is provided to maintain stability and to enhance biological and abiotic stress resistance. CONSTITUTION: A transgenic lawn grass with herbicide resistance and diseases resistance is obtained by transforming with a vector containing PepEST1 and Bar genes. The vector is transformed to grass embryonic developing callus by agrobacterium. The callus is cultured in a callus-inducing medium containing 0.2-0.8 mg/L of kinetin. The herbicide contains glufosinate as an active ingredient. The grass is Bentgrass.

Abstract translation: 目的：提供具有除草剂抗性和抗病性的转基因草坪草以保持稳定性和增强生物和非生物胁迫抗性。 构成：通过用含有PepEST1和Bar基因的载体转化获得具有除草剂抗性和抗病性的转基因草坪草。 载体通过农杆菌转化为草胚发育的愈伤组织。 将愈伤组织培养在含有0.2-0.8mg / L激动素的愈伤组织诱导培养基中。 除草剂含有草铵膦作为活性成分。 草是本草。

公开(公告)号：KR1020150065994A

公开(公告)日：2015-06-16

申请号：KR1020130150551

申请日：2013-12-05

Applicant: 전남대학교산학협력단

Inventor： 김정일 ,  박문영 ,  신아영 ,  한윤정

IPC: C12N15/82 ,  C12N15/29 ,  C07K14/415 ,  A01H5/00

Abstract: 본발명은애기장대() 유래 At3g52740 단백질코딩유전자를포함하는재조합벡터를식물세포에형질전환하여식물체의바이오매스생산성을증가시키거나또는종자수확량을증가시키는방법, 상기 At3g52740 단백질코딩유전자를포함하는재조합벡터를식물세포에형질전환하여식물체의바이오매스생산성이증가되거나또는종자수확량이증가된형질전환식물체의제조방법, 상기방법에의해제조된형질전환식물체및 이의종자, 상기 At3g52740 단백질코딩유전자를유효성분으로포함하는, 식물체의바이오매스생산성을증가시키거나또는종자수확량을증가시키기위한조성물, 상기애기장대 At3g52740 단백질코딩유전자유래프로모터, 상기프로모터를포함하는재조합식물발현벡터및 상기재조합식물발현벡터로형질전환된식물세포에관한것이다.

Abstract translation: 本发明涉及通过将包含来自拟南芥的At3g52740蛋白质编码基因的重组载体转化到植物细胞中来提高植物生物量生产力或种子产量的方法; 通过将包含At3g52740蛋白质编码基因的重组载体转化到植物细胞中来改善生物量生产力或种子产量的转化植物的制备方法; 通过该方法制备的转化植物和种子; 包含At3g52740蛋白质编码基因作为活性成分的组合物，用于提高植物中的生物量生产力或种子产量; 来自拟南芥At3g52740蛋白编码基因的启动子; 包含启动子的重组植物表达载体; 和转化到重组植物表达载体中的植物细胞。