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公开(公告)号：KR1020180085414A
公开(公告)日：2018-07-27
申请号：KR1020170008623
申请日：2017-01-18
Applicant: 전남대학교산학협력단
CPC classification number: C12N9/1029 , C12N15/8218 , C12N15/8269 , C12N15/8273 , C12Y203/01087
Abstract: 본발명은신규한세로토닌 N-아세틸전이효소2 유전자및 이의발현억제에의한형질전환식물체에관한것이다. 본발명은음지내성, 염저항성이탁월하거나광합성효율이우수하므로밀식재배조건또는염분이높은경작지에서도높은생산성을나타내는식물체로서유용하게사용될수 있다.
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公开(公告)号：KR101909369B1
公开(公告)日：2018-10-18
申请号：KR1020170008623
申请日：2017-01-18
Applicant: 전남대학교산학협력단
Abstract: 본발명은신규한세로토닌 N-아세틸전이효소2 유전자및 이의발현억제에의한형질전환식물체에관한것이다. 본발명은음지내성, 염저항성이탁월하거나광합성효율이우수하므로밀식재배조건또는염분이높은경작지에서도높은생산성을나타내는식물체로서유용하게사용될수 있다.
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公开(公告)号：KR1020150026420A
公开(公告)日：2015-03-11
申请号：KR1020130105227
申请日：2013-09-03
Applicant: 전남대학교산학협력단
Abstract: The present invention provides a method for manufacturing a new variety of Miscanthus sinensis having herbicide resistance by a transformation technique of Miscanthus sinensis using Agrobacterium tumefaciens. The present invention relates to a manufacturing method of a transgenic Miscanthus sinensis plant; to a transgenic Miscanthus sinensis plant manufactured by the method; and to seeds of the transgenic Miscanthus sinensis plant, wherein the method comprises the steps of (a) sterilizing mature seeds of Miscanthus sinensis of which seed coats have been removed; (b) placing the seeds of Miscanthus sinensis which have been sterilized on a callus induction medium to induce an embryogenic callus of Miscanthus sinensis; (c) inoculating an inoculation medium with Agrobacterium tumefaciens comprising a plant expression vector into which a selectable marker and an exotic gene are inserted and with the embryogenic callus of Miscanthus sinensis which has been induced, followed by performing a cocultivation process in a cocultivation medium to transform the embryogenic callus of Miscanthus sinensis; (d) selecting plant cells which have been cocultivated in a selection medium to which an antibiotic or a herbicide is added; and (e) performing a redifferentiation process of Miscanthus sinensis cells which have been selected in a redifferentiation medium.
Abstract translation: 本发明提供一种通过使用根癌土壤杆菌通过中国芒草的转化技术制造具有除草剂抗性的新品种的芒草的方法。 本发明涉及一种转基因芒草植物的制造方法， 通过该方法制造的转基因芒草植物; 以及所述转基因芒草种子，其中所述方法包括以下步骤：（a）对已经除去种皮的芒草的成熟种子进行灭菌; （b）将已经灭菌的芒草的种子放置在愈伤组织诱导培养基上以诱导中国芒草的胚发生愈伤组织; （c）用包含植物表达载体的土壤杆菌接种培养基，所述植物表达载体插入其中选择标记和外来基因并与已经诱导的芒草的胚发生愈伤组织接触，随后在共培养培养基中进行共培养过程 转化中国芒草的胚性愈伤组织; （d）选择在添加了抗生素或除草剂的选择培养基中共培养的植物细胞; 和（e）进行在再分化培养基中选择的芒草细胞的再分化过程。
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