公开(公告)号：KR100811745B1

公开(公告)日：2008-03-11

申请号：KR1020060064045

申请日：2006-07-07

Applicant: 조선대학교산학협력단

Inventor： 신송엽 ,  주만용 ,  함경수

IPC: C07K14/00

Abstract: 본 발명은 세포투과성 펩타이드인 Pep-1을 이용한 신규한 항균 펩타이드 Pep-1-K 및 그 용도에 관한 것으로, 구체적으로 세포투과성 펩타이드로 알려진 Pep-1의 2번째, 6번째 및 11번째의 아미노산인 글루탐산(Glu)을 라이신(Lys)으로 치환시켜 제조한 항균 펩타이드 Pep-1-K 및 그 용도에 관한 것이다. 본 발명의 Pep-1-K는 다양한 균주, 그 중에서도 그람-양성균, 그람-음성균 및 항생제에 내성을 가진 균주에서 강력한 항균활성을 나타낼 뿐 아니라 인간 적혈구 세포에서 어떠한 용혈활성도 나타내지 않기 때문에 무독성 항균제, 식품 방부제 및 화장품 첨가제로 유용하게 사용될 수 있다.
세포투과성 펩타이드, Pep-1, Pep-1-K

公开(公告)号：KR1020080004997A

公开(公告)日：2008-01-10

申请号：KR1020060064045

申请日：2006-07-07

Applicant: 조선대학교산학협력단

Inventor： 신송엽 ,  주만용 ,  함경수

IPC: C07K14/00

CPC classification number: C07K14/00 ,  A23L3/34635 ,  A61K8/64

Abstract: A novel synthetic or a recombinant antibacterial peptide is provided which shows strong antibacterial activity against various strains such as gram positive bacillus, gram negative bacillus and a strain having the resistance against antibiotics without any hemolytic activity in human erythrocyte cells, thereby being usefully used as a non-toxic antibacterial agent, a food preservative and a cosmetic additive. A synthetic or a recombinant antibacterial peptide is characterized in that it prepared by substituting at least one Glu at the 2nd, the 6th and the 11th of a cell-penetrating peptide Pep-1 with a positively charged basic amino acid such as arginine(Arg), histidine(His) and lysine(Lys) and it has the antibacterial activity against at least one strain selected from the group consisting of Bacillus subtilis KCTC 3068, Staphylococcus epidermidis KCTC 1917 and Staphylococcus aureus KCTC 1621. A non-toxic antibacterial agent comprises the antibacterial peptide as an effective ingredient.

Abstract translation: 提供了一种新型合成或重组抗菌肽，其对各种菌株如革兰氏阳性杆菌，革兰氏阴性芽孢杆菌和对人类红细胞中没有任何溶血活性的抗生素具有抗性的菌株具有强的抗菌活性，因此有用地用作 无毒抗菌剂，食品防腐剂和化妆品添加剂。 合成或重组抗菌肽的特征在于其通过用带正电荷的碱性氨基酸如精氨酸（Arg）在细胞穿透肽Pep-1的第2位，第6位和第11位取代至少一种Glu， ，组氨酸（His）和赖氨酸（Lys），并且其对选自枯草芽孢杆菌KCTC 3068，表皮葡萄球菌KCTC 1917和金黄色葡萄球菌KCTC1621的至少一种菌株具有抗菌活性。无毒性抗菌剂包括 抗菌肽作为有效成分。

公开(公告)号：KR100883815B1

公开(公告)日：2009-02-16

申请号：KR1020070094612

申请日：2007-09-18

Applicant: 조선대학교산학협력단

Inventor： 신송엽 ,  박가현 ,  함경수 ,  박윤경

IPC: A61K38/16 ,  A61P31/04

CPC classification number: Y02A50/481 ,  Y02A50/483 ,  A61K38/177 ,  A23L33/18 ,  A61K8/64 ,  A61Q17/005 ,  C07K14/705 ,  C12N15/113

Abstract: A model type antibiotic peptide to have abundant tryptophan is provided to show very low hemolytic activity in human RBC, to show very high bacterial cell specificity, to show high stability to human serum and to be usefully used as halogen free antimicrobial agent, food antiseptic and make-up additive. A synthesis or recombining antimicrobial peptide is selected from a group consisting of i) peptide selected from a group consisting of sequence number 1 to 4, ii) peptide in which lysine(Lys) is substituted for ornithine(Orn) from the peptide of i), iii) peptide in which leucine(Leu) is substituted for deer leucine(Nle) from the peptide of i) and iv) enantiomer peptide substituted for D-type amino acid corresponding all amino acids from the peptide of i) to iii). The peptide of ii) is written as sequence number 5.

Abstract translation: 提供了具有丰富色氨酸的模型型抗生素肽，以在人类RBC中显示非常低的溶血活性，显示出非常高的细菌细胞特异性，显示出对人血清的高度稳定性，并有效地用作无卤素抗微生物剂，食品防腐剂和 化妆添加剂。 合成或重组抗微生物肽选自i）选自序列号1至4的肽，ii）赖氨酸（Lys）取代来自i）的肽的鸟氨酸（Orn）的肽， ，iii）其中亮氨酸（Leu）从i）的肽替代鹿亮氨酸（Nle）的肽，和iv）取代相应于i）至iii）中所有肽的所有氨基酸的D型氨基酸的对映异构体肽。 ii）的肽序列号为5。