公开(公告)号：KR100490483B1

公开(公告)日：2005-05-17

申请号：KR1020010006352

申请日：2001-02-09

Applicant: 충남대학교산학협력단

Inventor： 임용표 ,  박종운 ,  장창순

IPC: C12N15/11

Abstract: 본 발명은 무사마귀병 내성 식물을 신속하게 선발하기 위한 DNA 마커, 상기 DNA 마커를 증폭시키기 위한 올리고뉴클레오타이드 프라이머 키트, 및 상기 프라이머 키트를 사용한 무사마귀병 내성 식물 품종 선발 방법에 관한 것으로서, 본 발명의 방법을 사용하여 무사마귀병균 레이스 특이적 내성 유전자를 가진 식물을 신속하고 안정적으로 선발할 수 있다.

公开(公告)号：KR1020020066106A

公开(公告)日：2002-08-14

申请号：KR1020010006352

申请日：2001-02-09

Applicant: 충남대학교산학협력단

Inventor： 임용표 ,  박종운 ,  장창순

IPC: C12N15/11

Abstract: PURPOSE: A DNA marker, primer kits, and a method for effective detection of a clubroot disease resistant plant are provided, thereby a clubroot disease resistant Chinese cabbage can be rapidly and stably selected without direct inoculation of clubroot disease-causing bacteria into the Chinese cabbage. CONSTITUTION: A DNA fragment contains at least a portion of the double helix nucleotide sequence represented by SEQ ID NO:1 and having the length of 256 bp, in which the DNA fragment is a DNA marker for selecting a clubroot disease resistant plant. The primer kits for selecting a clubroot disease resistant plant comprises the combination of oligonucleotides having the nucleotide sequence of SEQ ID NOs 4 to 9 and being complementary to a portion of the double helix nucleotide sequence. The method for elective detection of a clubroot disease resistant plant comprises the steps of: (a) extracting the genomic DNA from a plant, and detecting the amplified band; (b) designing one or more oligonucleotide primer complementary to a portion of the genomic DNA sequence using computer programs; (c) subjecting the genome isolated from the same kind of plant to PCR together with the primer; and (d) verifying whether the same kind of plant contains the DNA marker.

Abstract translation: 目的：提供DNA标记，引物试剂盒和有效检测棒状杆菌病抗性植物的方法，从而可以快速稳定地选择不加棒状病毒的大白菜，而不会直接接种丝状芽孢杆菌病引起的大白菜 。 构成：DNA片段含有由SEQ ID NO：1表示的双螺旋核苷酸序列的至少一部分，长度为256bp，其中DNA片段是选择丝状杆菌病抗性植物的DNA标记。 用于选择棒杆菌病抗性植物的引物组包括具有SEQ ID NO：4至9的核苷酸序列并与双螺旋核苷酸序列的一部分互补的寡核苷酸的组合。 选择性检测球根病抗性植物的方法包括以下步骤：（a）从植物中提取基因组DNA，并检测扩增条带; （b）使用计算机程序设计与所述基因组DNA序列的一部分互补的一个或多个寡核苷酸引物; （c）将从相同种类的植物分离的基因与引物一起进行PCR; 和（d）验证相同种类的植物是否含有DNA标记。