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    ＤＮＡ 결합에 의한 모세관 전기영동 이동도 어세이를 이용한 심근비대증 유발과 관련된 바이오마커인 ＮＦＡＴ의 검출법
    3.
    发明公开
    ＤＮＡ 결합에 의한 모세관 전기영동 이동도 어세이를 이용한 심근비대증 유발과 관련된 바이오마커인 ＮＦＡＴ의 검출법 有权
    通过DNA结合检测使用毛细电泳移动移动测定的NFAT的方法

    公开(公告)号：KR1020130039257A

    公开(公告)日：2013-04-19

    申请号：KR1020110103796

    申请日：2011-10-11

    Applicant: 한국과학기술연구원

    Inventor： 반은미 ,  박수현 ,  송은주 ,  유영숙

    IPC: G01N33/53 ,  C12Q1/68 ,  G01N33/52 ,  C12N15/11

    CPC classification number: C12Q1/6883 ,  C12Q2565/125 ,  G01N33/5302

    Abstract: PURPOSE: A method for detecting NFAT which is a biomarker for myocardial hypertrophy, using a capillary electrophoretic mobility shift assay by DNA binding is provided to effectively determine myocardial hypertrophy and to identify myocardial hypertrophy mechanism. CONSTITUTION: A method for detecting NFAT which is a biomarker for myocardial hypertrophy comprises: a step of reacting a quantitated nuclear extract and a fluorescent residue-labeled DNA in a buffer solution; a step of detecting DNA-conjugated NFAT using laser-induced fluorescence and isolating by capillary electrophoresis; and a step of quantitating detected activated T cell nuclear factor(NFAT) and a product thereof. The fluorescent residue-labeled DNA contains a probe with 5'-CAGCTAGGAAACAATTGGAAGTG-3' sequence. The probe specifically binds to NFAT.

    Abstract translation: 目的：提供一种检测作为心肌肥大的生物标志物的NFAT的方法，使用毛细管电泳迁移率变动测定法通过DNA结合，有效测定心肌肥大并鉴定心肌肥大机制。 构成：用于检测作为心肌肥大的生物标志物的NFAT的方法，包括：将定量的核提取物和荧光残基标记的DNA在缓冲溶液中反应的步骤; 使用激光诱导荧光检测DNA结合的NFAT并通过毛细管电泳分离的步骤; 以及定量检测到的活化T细胞核因子（NFAT）及其产物的步骤。 荧光残基标记的DNA含有具有5'-CAGCTAGGAAACAATTGGAAGTG-3'序列的探针。 探针特异性结合NFAT。

    모세관 전기 영동장치를 이용하여 세포에서 방출되는아질산염을 분석하는 방법
    4.
    发明授权
    모세관 전기 영동장치를 이용하여 세포에서 방출되는아질산염을 분석하는 방법 有权
    使用本应用程序中的应用程序在本应用程序中使用本应用程序

    公开(公告)号：KR100461921B1

    公开(公告)日：2004-12-18

    申请号：KR1020030002928

    申请日：2003-01-16

    Applicant: 한국과학기술연구원

    Inventor： 유영숙 ,  이지연 ,  반은미 ,  이세윤

    IPC: G01N27/447

    Abstract: PURPOSE: A method for analyzing a nitrite emitted from a cell by using a capillary electrophoresis is provided to reduce cost, time and labor for analyzing the reaction in a cell by using a capillary electrophoresis. CONSTITUTION: A method for analyzing a nitrite emitted from a cell by using a capillary electrophoresis comprises the steps of setting up a condition of a capillary electrophoresis, writing a concentration testing curve of a detecting peak versus a standard material by detecting a nitrite under the set condition, growing a cell on a medium, detecting a nitrite peak emitted from the cell by using a cell growing supernatant, and determining nitrite concentration by applying the nitrite peak to the concentration testing curve.

    Abstract translation: 目的：提供一种通过使用毛细管电泳分析从细胞中释放的亚硝酸盐的方法，以降低通过使用毛细管电泳来分析细胞中的反应的成本，时间和劳力。 本发明公开了一种利用毛细管电泳分析细胞发射的亚硝酸盐的方法，包括以下步骤：建立毛细管电泳条件，通过检测该组下的亚硝酸盐，写出检测峰对标准物质的浓度测试曲线 条件下，在培养基上生长细胞，通过使用细胞生长上清液检测从细胞发射的亚硝酸盐峰值，并通过将亚硝酸盐峰值应用于浓度测试曲线来测定亚硝酸盐浓度。

    트리톤 Ｘ－100 첨가에 의한 세포 내 ｍｉＲＮＡ의 추출 효율의 증가 방법
    7.
    发明公开
    트리톤 Ｘ－100 첨가에 의한 세포 내 ｍｉＲＮＡ의 추출 효율의 증가 방법 有权
    通过添加TRITON X-100提高细胞提取MIRNA提取效率的方法

    公开(公告)号：KR1020140094905A

    公开(公告)日：2014-07-31

    申请号：KR1020130007541

    申请日：2013-01-23

    Applicant: 한국과학기술연구원

    Inventor： 송은주 ,  반은미 ,  채동규

    IPC: C12Q1/68 ,  C12N15/113

    CPC classification number: C12N15/1003 ,  C12Q1/6816 ,  C12Q1/6806 ,  C12N15/113 ,  C12Q1/6813 ,  C12Q2525/207 ,  C12Q2527/125

    Abstract: The present invention relates to a method for enhancing miRNA extraction efficiency which exists in a cell in the minimum amount by adding a triton X-100. The present invention is able to quantitatively and quickly analyze a miRNA which exists in a sample in the minimum amount. The miRNA extraction method which adds a triton X-100, provided by the present invention, is able to enhance extraction efficiency more than two times in comparison with a case of using only a trisol reagent.

    Abstract translation: 本发明涉及一种增加miRNA提取效率的方法，该方法通过加入triton X-100以最小的量存在于细胞中。 本发明能够以最小的量定量和快速地分析样品中存在的miRNA。 与仅使用三羟甲基试剂的情况相比，添加本发明提供的triton X-100的miRNA提取方法能够提高提取效率两倍以上。

    형광검출기를 포함한 모세관 전기영동법을 이용한 miRNA 저해제의 효능 평가 방법
    9.
    发明公开
    형광검출기를 포함한 모세관 전기영동법을 이용한 miRNA 저해제의 효능 평가 방법 有权
    毛细管电泳法检测miRNA抑制剂效果的评价方法，包括荧光检测器

    公开(公告)号：KR1020170054948A

    公开(公告)日：2017-05-18

    申请号：KR1020150157739

    申请日：2015-11-10

    Applicant: 한국과학기술연구원

    Inventor： 송은주 ,  채동규 ,  반은미 ,  유영숙

    IPC: C12Q1/68 ,  C12Q1/66 ,  C12N15/113

    Abstract: 본발명은 miRNA 저해제에의한 miRNA 발현양의변화를, 형광을띈 DNA 프로브와세포내 존재하는 miRNA와의혼성화(hybridization)시켜형광검출기가창착된모세관전기영동(CE/LIF) 시스템을이용하여 miRNA 발현양을측정하되, 특히 DNA 프로브의농도를 miRNA 저해제에의해방해를받지않는농도로최적화하여사용함으로써, miRNA 발현양을정확하게측정할수 있어다양한질환을확인, 판정및 발명기작을규명하는데유용하다.

    Abstract translation: 本发明涉及通过使用毛细管电泳（CE / LIF）系统检测miRNA表达的方法，其中荧光检测器与存在于细胞中的荧光DNA探针和miRNA杂交， 但是表达水平的测量，通过使用特别针对DNA探针不被miRNA抑制剂被干扰的浓度的浓度最优化，我们可以精确地测量miRNA表达的量是在识别检查各种疾病是有用的，被确定，并且在本发明的机制。

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