Abstract:
PURPOSE: A method for detecting NFAT which is a biomarker for myocardial hypertrophy, using a capillary electrophoretic mobility shift assay by DNA binding is provided to effectively determine myocardial hypertrophy and to identify myocardial hypertrophy mechanism. CONSTITUTION: A method for detecting NFAT which is a biomarker for myocardial hypertrophy comprises: a step of reacting a quantitated nuclear extract and a fluorescent residue-labeled DNA in a buffer solution; a step of detecting DNA-conjugated NFAT using laser-induced fluorescence and isolating by capillary electrophoresis; and a step of quantitating detected activated T cell nuclear factor(NFAT) and a product thereof. The fluorescent residue-labeled DNA contains a probe with 5'-CAGCTAGGAAACAATTGGAAGTG-3' sequence. The probe specifically binds to NFAT.
Abstract:
PURPOSE: A biomarker for myocardial hypertrophy diagnosis and a myocardial hypertrophy diagnosis method using the same are provided to judge myocardial hypertrophy from the biological material. CONSTITUTION: A biomarker for myocardial hypertrophy diagnosis comprises PDZ and LIM domain protein which have amino acid sequence described in sequence number 1(SEQ ID NO:1), ADP-ribosyl hydrolase which has amino acid sequence described in sequence number 2, peptidyl - prolyl cis-trans isomerase E which has amino acid sequence described in sequence number 3, translation extension element 1 delta isoform b of eukaryotic cells which has amino acid sequence described in sequence number 4, Ran- specific GTPase- activator protein which has amino acid sequence described in sequence number 5, ATP synthase subunit beta which has amino acid sequence described in sequence number 6, mitochondrial precursor, or isoform 1 of astrocytic phosphor protein PEA-15 which has amino acid sequence described in sequence number 7.
Abstract:
본 발명은 세포 내 STAT1의 인산화 수준을 측정하는 방법에 관한 것으로서, 더욱 상세하게는 인산화된 STAT1을 인식하는 항체와 FITC가 결합된 2차 항체를 이용하여 사이토카인에 의해 자극된 세포에서 STAT1의 인산화 정도를 신속하게 측정하기 위한 세포 내 STAT1의 인산화 수준을 측정하는 방법에 관한 것이다. 이러한 본 발명의 세포 내 STAT1의 인산화 수준 측정 방법은 사이토카인에 의해 자극된 세포에서 STAT1 단백질의 인산화 정도와의 상대적 비교를 통하여 측정하고자 하는 세포의 인산화 수준을 신속하고 정밀하게 측정 가능하여 STAT1 이외의 다른 단백질들의 분석에도 적용 가능하고, 약물 개발 등에 유용하리라 기대된다.
Abstract:
PURPOSE: A method for analyzing a nitrite emitted from a cell by using a capillary electrophoresis is provided to reduce cost, time and labor for analyzing the reaction in a cell by using a capillary electrophoresis. CONSTITUTION: A method for analyzing a nitrite emitted from a cell by using a capillary electrophoresis comprises the steps of setting up a condition of a capillary electrophoresis, writing a concentration testing curve of a detecting peak versus a standard material by detecting a nitrite under the set condition, growing a cell on a medium, detecting a nitrite peak emitted from the cell by using a cell growing supernatant, and determining nitrite concentration by applying the nitrite peak to the concentration testing curve.