公开(公告)号：KR100461772B1

公开(公告)日：2004-12-14

申请号：KR1020010081966

申请日：2001-12-20

Applicant: 한국과학기술연구원

Inventor： 신평균 ,  배희경 ,  송방호

IPC: C12N1/20

Abstract: PURPOSE: A microorganism E. coli SB012 (KFCC 11203) sensitive to organic solvents and a method of preparation thereof are provided, thereby detecting toxicity more sensitively. CONSTITUTION: A microorganism E. coli SB012 (KFCC 11203) having improved sensitivity to organic solvents is prepared by modification or deletion of lipopolysaccharide in an extracellular membrane of E. coli SB01, wherein the modification or deletion of lipopolysaccharide in the extracellular membrane of E. coli SB01 is caused by treatment of E. coli SB01 with ethyl-methane-sulfonate(EMS), and repeatedly culturing the EMS-treated E. coli SB01 in a medium containing an antimicrobial agent and an organic solvent, wherein the antimicrobial agent is tetracycline and the organic solvent is para-xylene or bile acid salts.

Abstract translation: 目的：提供对有机溶剂敏感的微生物大肠杆菌SB012（KFCC 11203）及其制备方法，由此更灵敏地检测毒性。 组成：对有机溶剂具有改善的敏感性的微生物大肠杆菌SB012（KFCC 11203）是通过修饰或缺失大肠杆菌SB01的细胞外膜中的脂多糖来制备的，其中E的细胞外膜中的脂多糖的修饰或缺失。 大肠杆菌SB01是通过用乙基 - 甲磺酸盐（EMS）处理大肠杆菌SB01并且在含有抗微生物剂和有机溶剂的培养基中反复培养EMS-处理的大肠杆菌SB01而引起的，其中抗微生物剂是四环素 有机溶剂是对二甲苯或胆汁酸盐。

公开(公告)号：KR1020030051067A

公开(公告)日：2003-06-25

申请号：KR1020010081966

申请日：2001-12-20

Applicant: 한국과학기술연구원

Inventor： 신평균 ,  배희경 ,  송방호

IPC: C12N1/20

Abstract: PURPOSE: A microorganism E. coli SB012 (KFCC 11203) sensitive to organic solvents and a method of preparation thereof are provided, thereby detecting toxicity more sensitively. CONSTITUTION: A microorganism E. coli SB012 (KFCC 11203) having improved sensitivity to organic solvents is prepared by modification or deletion of lipopolysaccharide in an extracellular membrane of E. coli SB01, wherein the modification or deletion of lipopolysaccharide in the extracellular membrane of E. coli SB01 is caused by treatment of E. coli SB01 with ethyl-methane-sulfonate(EMS), and repeatedly culturing the EMS-treated E. coli SB01 in a medium containing an antimicrobial agent and an organic solvent, wherein the antimicrobial agent is tetracycline and the organic solvent is para-xylene or bile acid salts.

Abstract translation: 目的：提供对有机溶剂敏感的微生物大肠杆菌SB012（KFCC 11203）及其制备方法，从而更灵敏地检测毒性。 构成：通过在大肠杆菌SB01的细胞外膜中修饰或缺失脂多糖来制备对有机溶剂具有改善的敏感性的微生物大肠杆菌SB012（KFCC 11203），其中在细胞外膜中的脂多糖的修饰或缺失。 大肠杆菌SB01是通过用甲基磺酸乙酯（EMS）处理大肠杆菌SB01引起的，并且在含有抗微生物剂和有机溶剂的培养基中反复培养EMS处理的大肠杆菌SB01，其中抗微生物剂是四环素 有机溶剂为对二甲苯或胆汁酸盐。

公开(公告)号：KR1020020041603A

公开(公告)日：2002-06-03

申请号：KR1020000071267

申请日：2000-11-28

Applicant: 한국과학기술연구원

Inventor： 신평균 ,  배희경 ,  오현주 ,  송방호

IPC: C12N15/65

CPC classification number: C12N15/65 ,  C12N15/70 ,  C12Q2600/142

Abstract: PURPOSE: A recombinant plasmid containing luxCDABE, E. coli SB01 (KFCC 11176) transformed therewith, and the use thereof are provided, therefore the stability of the recombinant plasmid in E. coli can be increased to use it for the detection of toxicity. CONSTITUTION: The recombinant plasmid pBS1 contains a vector pKLJ12 containing luxCDABE derived from Photorhabdus luminescens. E. coli SB01 KFCC 11176 is produced by transforming E. coli DH5alpha with the recombinant plasmid pBS1. A method for detecting toxicity in a sample solution comprises culturing the transformed microorganism E. coli SB01 KFCC 11176 in the sample solution in a continuous fermenter, and analyzing the luminescence of the sample solution.

Abstract translation: 目的：提供含有luxCDABE，大肠杆菌SB01（KFCC 11176）转化的重组质粒及其用途，因此可以增加重组质粒在大肠杆菌中的稳定性，以用于检测毒性。 构成：重组质粒pBS1含有一种载体pKLJ12，其含有源自Photorhabdus luminescens的luxCDABE。 大肠杆菌SB01KFCC 11176是用重组质粒pBS1转化大肠杆菌DH5α产生的。 用于检测样品溶液中的毒性的方法包括在连续发酵罐中的样品溶液中培养转化的大肠杆菌SB01KFCC 11176，并分析样品溶液的发光。