公开(公告)号：WO1995018676A1

公开(公告)日：1995-07-13

申请号：PCT/US1995000413

申请日：1995-01-11

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  ZUREK, Thomas, F. ,  HANLEY, Kathleen, A. ,  PEPE, Curtis, J.

IPC: B01L07/00

CPC classification number: B01L7/52 ,  G05D23/138 ,  Y10T436/115831 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/12 ,  Y10T436/25

Abstract: The method and apparatus for thermal cycling of nucleic acid assys includes a blended fluid stream produced from a plurality of constant velocity, constant volume, constant temperature fluid streams wherein to provide a variable temperature, constant velocity, constant volume fluid stream which is introduced into a sample chamber for heating and cooling samples contained therein. By diverting and altering the ratio of the constant temperature fluid streams relative to one another, the blended fluid stream is rapidly variable in temperature, providing for almost instantaneous temperature change within the environment defined by the sample chamber.

Abstract translation: 核酸组件的热循环的方法和装置包括由多个等速恒定体积的恒温流体流产生的混合流体流，其中提供可变温度恒定速度的恒定体积流体流，其被引入到 样品室，用于加热和冷却其中所含的样品。 通过转移和改变恒温流体流相对于彼此的比例，混合的流体流在温度上快速变化，从而在由样品室限定的环境内提供几乎瞬间的温度变化。

公开(公告)号：WO1995011083A2

公开(公告)日：1995-04-27

申请号：PCT/US1994012125

申请日：1994-10-21

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  HANLEY, Kathleen, A. ,  HOFFERBERT, A., David ,  LEE, Helen, H. ,  PEPE, Curtis, J. ,  PERKO, Timothy, J. ,  ZUREK, Thomas, F.

IPC: B01L07/00

CPC classification number: B01L3/50825 ,  B01L7/52

Abstract: A disposable reaction vessel for performing nucleic acid amplification assay. The disposable reaction vessel has a penetrable cap that can be penetrated by an automated pipettor to aspirate a portion of an amplified reaction product. The disposable reaction vessel contains the reagents necessary to perform a nucleic acid amplification assay. A patient speciment is added to the unit dose reagents in the disposable reaction vessel and the penetrable cap is closed. The disposable reaction vessel containing the reaction mixture and the specimen undergoes amplification, typically by placing it in a thermal cycler. After amplification the intact disposable reaction vessel is transferred to an automated analyzer where an automated pipettor penetrates the closure membrane and aspirates a portion of the amplified sample for further processing, without removal of the reaction vessel cap. This avoids the generation of potentially contaminating aerosols or droplets.

Abstract translation: 用于进行核酸扩增测定的一次性反应容器。 一次性反应容器具有可穿透的帽，其可被自动移液器穿透以吸收一部分经扩增的反应产物。 一次性反应容器含有执行核酸扩增测定所必需的试剂。 将患者物质加入到一次性反应容器中的单位剂量试剂中，并将可穿透盖封闭。 含有反应混合物和样品的一次性反应容器通常通过将其放置在热循环仪中进行扩增。 扩增后，将完整的一次性反应容器转移至自动分析器，其中自动移液器渗透封闭膜并抽吸一部分扩增的样品用于进一步处理，而不需要移除反应容器盖。 这避免了潜在的污染气溶胶或液滴的产生。

公开(公告)号：EP1121200B1

公开(公告)日：2006-12-20

申请号：EP99951904.4

申请日：1999-10-12

Applicant: ABBOTT LABORATORIES

Inventor： BACH, Mark, C. ,  BAY, Daniel ,  CARTER, Michael, K. ,  CLEMENS, John, M. ,  DAHLKE, Daniel, G. ,  GALITZ, Charles, M. ,  GRAY, Robert, C. ,  HALAKA, Folim ,  HERCHENBACH, Steve ,  KUKLA, Ronald, E. ,  PEPE, Curtis, J. ,  PIERCE, Mark ,  SAFAR, Scott, G. ,  TOTH, Julius ,  ZUCK, Gary

IPC: B01L7/00 ,  G01N35/02

CPC classification number: B01L7/52 ,  G01N35/02 ,  G01N2035/00366 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998

Abstract: A method of performing a determination of an item of interest in a sample using a single structure is disclosed. A sample is provided accesssible to the single structure. A first container for processing the sample is placed in a first process path on the single structure. The sample is transferred to the first container in the first process path. A reagent is added to the first container in the first process path. Contents of the first container is mixed in the first process path. The item of interest in the sample is separated from the contents of the first container in the first process path. The separated item of interest in the sample is transferred from the first container in the first process path to a second container in a second process path on the single structure. Contents of the second container are brought to a first temperature different from a temperature of the first process path in the second process path. The item of interest in the second container is detected in the second process path.

公开(公告)号：EP0739241B1

公开(公告)日：1998-08-12

申请号：EP95906818.0

申请日：1995-01-11

Applicant: Abbott Laboratories

Inventor： ZUREK, Thomas, F. ,  HANLEY, Kathleen, A. ,  PEPE, Curtis, J.

IPC: B01L7/00 ,  G05D23/13

CPC classification number: B01L7/52 ,  G05D23/138 ,  Y10T436/115831 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/12 ,  Y10T436/25

Abstract: The method and apparatus for thermal cycling of nucleic acid assys includes a blended fluid stream produced from a plurality of constant velocity, constant volume, constant temperature fluid streams wherein to provide a variable temperature, constant velocity, constant volume fluid stream which is introduced into a sample chamber for heating and cooling samples contained therein. By diverting and altering the ratio of the constant temperature fluid streams relative to one another, the blended fluid stream is rapidly variable in temperature, providing for almost instantaneous temperature change within the environment defined by the sample chamber.

Abstract translation: 用于核酸组件的热循环的方法和设备包括由多个恒速，恒定体积，恒温流体流产生的混合流体流，其中为了提供可变温度，恒定速度，恒定体积流体流，其被引入到 样品室用于加热和冷却其中包含的样品。 通过改变和改变恒温流体流相对于彼此的比例，混合流体流的温度快速变化，在由样品室限定的环境内提供几乎瞬时的温度变化。

公开(公告)号：EP1121200A1

公开(公告)日：2001-08-08

申请号：EP99951904.4

申请日：1999-10-12

Applicant: ABBOTT LABORATORIES

Inventor： BACH, Mark, C. ,  BAY, Daniel ,  CARTER, Michael, K. ,  CLEMENS, John, M. ,  DAHLKE, Daniel, G. ,  GALITZ, Charles, M. ,  GRAY, Robert, C. ,  HALAKA, Folim ,  HERCHENBACH, Steve ,  KUKLA, Ronald, E. ,  PEPE, Curtis, J. ,  PIERCE, Mark ,  SAFAR, Scott, G. ,  TOTH, Julius ,  ZUCK, Gary

IPC: B01L7/00 ,  G01N35/02

CPC classification number: B01L7/52 ,  G01N35/02 ,  G01N2035/00366 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998

Abstract: A method of performing a determination of an item of interest in a sample using a single structure is disclosed. A sample is provided accesssible to the single structure. A first container for processing the sample is placed in a first process path on the single structure. The sample is transferred to the first container in the first process path. A reagent is added to the first container in the first process path. Contents of the first container is mixed in the first process path. The item of interest in the sample is separated from the contents of the first container in the first process path. The separated item of interest in the sample is transferred from the first container in the first process path to a second container in a second process path on the single structure. Contents of the second container are brought to a first temperature different from a temperature of the first process path in the second process path. The item of interest in the second container is detected in the second process path.

公开(公告)号：EP0739241A1

公开(公告)日：1996-10-30

申请号：EP95906818.0

申请日：1995-01-11

Applicant: Abbott Laboratories

Inventor： ZUREK, Thomas, F. ,  HANLEY, Kathleen, A. ,  PEPE, Curtis, J.

IPC: C12N15 ,  B01L7 ,  C12M1 ,  C12Q1 ,  G05D23

CPC classification number: B01L7/52 ,  G05D23/138 ,  Y10T436/115831 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/12 ,  Y10T436/25

Abstract: The method and apparatus for thermal cycling of nucleic acid assys includes a blended fluid stream produced from a plurality of constant velocity, constant volume, constant temperature fluid streams wherein to provide a variable temperature, constant velocity, constant volume fluid stream which is introduced into a sample chamber for heating and cooling samples contained therein. By diverting and altering the ratio of the constant temperature fluid streams relative to one another, the blended fluid stream is rapidly variable in temperature, providing for almost instantaneous temperature change within the environment defined by the sample chamber.

公开(公告)号：EP0724483A1

公开(公告)日：1996-08-07

申请号：EP95900401.0

申请日：1994-10-21

Applicant: Abbott Laboratories

Inventor： HANLEY, Kathleen, A. ,  HOFFERBERT, A., David ,  LEE, Helen, H. ,  PEPE, Curtis, J. ,  PERKO, Timothy, J. ,  ZUREK, Thomas, F.

IPC: G01N33 ,  B01L3 ,  B01L7 ,  B01L11 ,  C12M1 ,  C12N15 ,  C12Q1

CPC classification number: B01L3/50825 ,  B01L7/52

Abstract: A disposable reaction vessel for performing nucleic acid amplification assay. The disposable reaction vessel has a penetrable cap that can be penetrated by an automated pipettor to aspirate a portion of an amplified reaction product. The disposable reaction vessel contains the reagents necessary to perform a nucleic acid amplification assay. A patient speciment is added to the unit dose reagents in the disposable reaction vessel and the penetrable cap is closed. The disposable reaction vessel containing the reaction mixture and the specimen undergoes amplification, typically by placing it in a thermal cycler. After amplification the intact disposable reaction vessel is transferred to an automated analyzer where an automated pipettor penetrates the closure membrane and aspirates a portion of the amplified sample for further processing, without removal of the reaction vessel cap. This avoids the generation of potentially contaminating aerosols or droplets.