公开(公告)号：WO1995011083A2

公开(公告)日：1995-04-27

申请号：PCT/US1994012125

申请日：1994-10-21

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  HANLEY, Kathleen, A. ,  HOFFERBERT, A., David ,  LEE, Helen, H. ,  PEPE, Curtis, J. ,  PERKO, Timothy, J. ,  ZUREK, Thomas, F.

IPC: B01L07/00

CPC classification number: B01L3/50825 ,  B01L7/52

Abstract: A disposable reaction vessel for performing nucleic acid amplification assay. The disposable reaction vessel has a penetrable cap that can be penetrated by an automated pipettor to aspirate a portion of an amplified reaction product. The disposable reaction vessel contains the reagents necessary to perform a nucleic acid amplification assay. A patient speciment is added to the unit dose reagents in the disposable reaction vessel and the penetrable cap is closed. The disposable reaction vessel containing the reaction mixture and the specimen undergoes amplification, typically by placing it in a thermal cycler. After amplification the intact disposable reaction vessel is transferred to an automated analyzer where an automated pipettor penetrates the closure membrane and aspirates a portion of the amplified sample for further processing, without removal of the reaction vessel cap. This avoids the generation of potentially contaminating aerosols or droplets.

Abstract translation: 用于进行核酸扩增测定的一次性反应容器。 一次性反应容器具有可穿透的帽，其可被自动移液器穿透以吸收一部分经扩增的反应产物。 一次性反应容器含有执行核酸扩增测定所必需的试剂。 将患者物质加入到一次性反应容器中的单位剂量试剂中，并将可穿透盖封闭。 含有反应混合物和样品的一次性反应容器通常通过将其放置在热循环仪中进行扩增。 扩增后，将完整的一次性反应容器转移至自动分析器，其中自动移液器渗透封闭膜并抽吸一部分扩增的样品用于进一步处理，而不需要移除反应容器盖。 这避免了潜在的污染气溶胶或液滴的产生。

公开(公告)号：EP0724483A1

公开(公告)日：1996-08-07

申请号：EP95900401.0

申请日：1994-10-21

Applicant: Abbott Laboratories

Inventor： HANLEY, Kathleen, A. ,  HOFFERBERT, A., David ,  LEE, Helen, H. ,  PEPE, Curtis, J. ,  PERKO, Timothy, J. ,  ZUREK, Thomas, F.

IPC: G01N33 ,  B01L3 ,  B01L7 ,  B01L11 ,  C12M1 ,  C12N15 ,  C12Q1

CPC classification number: B01L3/50825 ,  B01L7/52

Abstract: A disposable reaction vessel for performing nucleic acid amplification assay. The disposable reaction vessel has a penetrable cap that can be penetrated by an automated pipettor to aspirate a portion of an amplified reaction product. The disposable reaction vessel contains the reagents necessary to perform a nucleic acid amplification assay. A patient speciment is added to the unit dose reagents in the disposable reaction vessel and the penetrable cap is closed. The disposable reaction vessel containing the reaction mixture and the specimen undergoes amplification, typically by placing it in a thermal cycler. After amplification the intact disposable reaction vessel is transferred to an automated analyzer where an automated pipettor penetrates the closure membrane and aspirates a portion of the amplified sample for further processing, without removal of the reaction vessel cap. This avoids the generation of potentially contaminating aerosols or droplets.