公开(公告)号：JP2012228268A

公开(公告)日：2012-11-22

申请号：JP2012167346

申请日：2012-07-27

Applicant: Fluidigm Corp ,  フルイディグム コーポレイション

Inventor： CHOU HOU-PU ,  DARIDON ANTOINE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY ,  JAVADI SHERVIN ,  LIAU YISH-HANN ,  MANGER IAN D ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  THRONDSET WILLIAM

IPC: C12M1/34 ,  G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M3/00 ,  C12N1/00 ,  C12N5/071 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

CPC classification number: C12M1/34 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0655 ,  C12M21/06 ,  C12M23/16 ,  G01N15/1484 ,  G01N33/4833 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2015/1493 ,  G02B21/32

Abstract: PROBLEM TO BE SOLVED: To provide novel methods for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles.SOLUTION: The invention includes apparatus, methods, and kits for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention includes apparatus, methods, and kits for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order, and employed for any suitable number of times within a system.

Abstract translation: 要解决的问题：提供微流控操作和/或分析颗粒如细胞，病毒，细胞器，珠粒和/或囊泡的新方法。 解决方案：本发明包括用于微流控操作和/或检测颗粒例如细胞和/或珠的装置，方法和试剂盒。 本发明包括用于微粒操作和/或分析颗粒如细胞，病毒，细胞器，珠粒和/或囊泡的装置，方法和试剂盒。 本发明还提供用于进行这些操作和分析的微流体机理。 这些机制可以实现颗粒的控制输入，运动/定位，保留/定位，处理，测量，释放和/或输出。 此外，这些机构可以以任何合适的顺序组合，并且在系统内使用任何合适的次数。 版权所有（C）2013，JPO＆INPIT

公开(公告)号：JP2010042020A

公开(公告)日：2010-02-25

申请号：JP2009236738

申请日：2009-10-13

Applicant: Fluidigm Corp ,  フルイディグム コーポレイション

Inventor： CHOU HOU-PU ,  DARIDON ANTOINE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY ,  JAVADI SHERVIN ,  LIAU YISH-HANN ,  MANGER IAN D ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  THRONDSET WILLIAM

IPC: C12Q1/02 ,  G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  G01N15/02 ,  G01N15/14

CPC classification number: C12M1/34 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0655 ,  C12M21/06 ,  C12M23/16 ,  G01N15/1484 ,  G01N33/4833 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2015/1493 ,  G02B21/32

Abstract: PROBLEM TO BE SOLVED: To provide a new method for microfluidic manipulation and/or analysis of particles (e.g., cells, viruses, organelles, beads, and/or vesicles). SOLUTION: A microfluidic particle-analysis system includes a device, a method, and a kit for the microfluidic manipulation and/or detection of the particles such as cells and/or beads. The system includes the device, the method and the kit for the microfluidic manipulation and/or analysis of the particles such as the cells, viruses, organelles, beads and/or vesicles, and microfluidic mechanisms are provided for performing such the manipulations and analyses. Such mechanisms enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of the particles. Further, these mechanisms can be combined in any suitable order and employed in any suitable number of times within the system. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供用于微粒操作和/或分析颗粒（例如细胞，病毒，细胞器，珠粒和/或囊泡）的新方法。 解决方案：微流体颗粒分析系统包括用于微流体操纵和/或检测诸如细胞和/或珠粒的微流体的装置，方法和试剂盒。 该系统包括用于微粒操作和/或分析细胞，病毒，细胞器，珠粒和/或囊泡的微流体操作和/或分析的装置，方法和试剂盒，以及用于进行这种操作和分析的微流体机制。 这样的机构使得能够控制输入，移动/定位，保留/定位，处理，测量，释放和/或输出颗粒。 此外，这些机构可以以任何合适的顺序组合并且在系统内以任何合适的次数使用。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：WO0230486A3

公开(公告)日：2002-07-04

申请号：PCT/US0142635

申请日：2001-10-12

Applicant: FLUIDIGM CORP ,  MANAGER IAN DAVID ,  HAO CUNSHENG CASEY ,  UNGER MARC ALEXANDER

Inventor： MANAGER IAN DAVID ,  HAO CUNSHENG CASEY ,  UNGER MARC ALEXANDER

IPC: A61L15/38 ,  A61L15/40 ,  B01L3/00 ,  B01L3/02 ,  B81B1/00 ,  B81C1/00 ,  G01N30/72 ,  H01J49/04 ,  G01N24/00 ,  B01D59/44 ,  G01N30/00 ,  G01N33/00 ,  G03F7/00 ,  H01J49/40

CPC classification number: G01N1/10 ,  B01L3/0241 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0481 ,  G01N30/7266 ,  H01J49/0018 ,  H01J49/165 ,  Y10T436/117497 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: This invention provides a microfluidic sample injection apparatus (14, 100) for injecting a fluid sample into an analytical device (120) and a method for using the same. The microfluidic sample injection apparatus (14, 100) comprises a microfluidic device (100) and an integrated sample injection capillary (14) which is in fluid communication with a fluid flow channel of the microfluidic device (100).

Abstract translation: 本发明提供了一种用于将流体样品注入分析装置（120）的微流体样品注射装置（14,100）及其使用方法。 微流体样品注射设备（14,100）包括微流体装置（100）和与微流体装置（100）的流体流动通道流体连通的整合样品注射毛细管（14）。

公开(公告)号：WO0230486A9

公开(公告)日：2003-02-06

申请号：PCT/US0142635

申请日：2001-10-12

Applicant: FLUIDIGM CORP ,  MANAGER IAN DAVID ,  HAO CUNSHENG CASEY ,  UNGER MARC ALEXANDER

Inventor： MANAGER IAN DAVID ,  HAO CUNSHENG CASEY ,  UNGER MARC ALEXANDER

IPC: A61L15/38 ,  A61L15/40 ,  B01L3/00 ,  B01L3/02 ,  B81B1/00 ,  B81C1/00 ,  G01N30/72 ,  H01J49/04 ,  G01N24/00 ,  B01D59/44 ,  G01N30/00 ,  G01N33/00 ,  G03F7/00 ,  H01J49/40

CPC classification number: G01N1/10 ,  B01L3/0241 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0481 ,  G01N30/7266 ,  H01J49/0018 ,  H01J49/165 ,  Y10T436/117497 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: This invention provides a microfluidic sample injection apparatus (14, 100) for injecting a fluid sample into an analytical device (120) and a method for using the same. The microfluidic sample injection apparatus (14, 100) comprises a microfluidic device (100) and an integrated sample injection capillary (14) which is in fluid communication with a fluid flow channel of the microfluidic device (100).

Abstract translation: 本发明提供一种用于将流体样品注入分析装置（120）的微流体样品注射装置（14,100）及其使用方法。 微流体样品注射装置（14,100）包括微流体装置（100）和与微流体装置（100）的流体流动通道流体连通的集成样品注入毛细管（14）。

公开(公告)号：AU2003224817B2

公开(公告)日：2008-11-06

申请号：AU2003224817

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： DARIDON ANTOINE ,  MANGER IAN D ,  LIAU YISH-HANN ,  NASSEF HANY RAMEZ ,  THRONDSET WILLIAM ,  CHOU HOU-PU ,  HAO CUNSHENG CASEY ,  NORTON PIERCE ,  FOWLER BRIAN ,  FARRELL KEVIN ,  JAVADI SHERVIN

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

公开(公告)号：CA2480728A1

公开(公告)日：2003-10-16

申请号：CA2480728

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： MANGER IAN D ,  THRONDSET WILLIAM ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY ,  LIAU YISH-HANN ,  JAVADI SHERVIN ,  DARIDON ANTOINE ,  CHOU HOU-PU

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

Abstract: The invention provides systems (2000), including microfluidic mechanisms, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. These mechanisms may enable controlle d input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any number of suitable times in the system to allow particles to be sorted, cultured, mixed, treate d, and/or assayed, among others. These combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.

公开(公告)号：AU2003224817A1

公开(公告)日：2003-10-20

申请号：AU2003224817

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： JAVADI SHERVIN ,  LIAU YISH-HANN ,  MANGER IAN D ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  THRONDSET WILLIAM ,  CHOU HOU-PU ,  DARIDON ANTOINE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

公开(公告)号：AU1189702A

公开(公告)日：2002-04-22

申请号：AU1189702

申请日：2001-10-12

Applicant: FLUIDIGM CORP

Inventor： MANAGER IAN DAVID ,  HAO CUNSHENG CASEY ,  UNGER MARC ALEXANDER

IPC: A61L15/38 ,  A61L15/40 ,  B01L3/00 ,  B01L3/02 ,  B81B1/00 ,  B81C1/00 ,  G01N30/72 ,  H01J49/04

公开(公告)号：EP1336097A4

公开(公告)日：2006-02-01

申请号：EP01979990

申请日：2001-10-12

Applicant: FLUIDIGM CORP

Inventor： MANGER IAN DAVID ,  HAO CUNSHENG CASEY ,  UNGER MARC ALEXANDER

IPC: B01L3/00 ,  A61L15/38 ,  A61L15/40 ,  B01L3/02 ,  B81B1/00 ,  B81C1/00 ,  G01N30/72 ,  H01J49/04

CPC classification number: G01N1/10 ,  B01L3/0241 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0481 ,  G01N30/7266 ,  H01J49/0018 ,  H01J49/165 ,  Y10T436/117497 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: This invention provides a microfluidic sample injection apparatus (14, 100) for injecting a fluid sample into an analytical device (120) and a method for using the same. The microfluidic sample injection apparatus (14, 100) comprises a microfluidic device (100) and an integrated sample injection capillary (14) which is in fluid communication with a fluid flow channel of the microfluidic device (100).

Abstract translation: 本发明提供微流体装置及其使用方法。 本发明的微流体装置包括第一弹性层，弹性层内的流体流动通道; 以及用于将流体样品从流体流动通道提供到分析装置的装置。 本发明还提供了一种包括这种微流体装置和分析装置的分析装置。

公开(公告)号：EP1499706A4

公开(公告)日：2010-11-03

申请号：EP03721509

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： CHOU HOU-PU ,  DARIDON ANTOINE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY ,  JAVADI SHERVIN ,  LIAU YISH-HANN ,  MANGER IAN D ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  THRONDSET WILLIAM

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

CPC classification number: C12M1/34 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0655 ,  C12M21/06 ,  C12M23/16 ,  G01N15/1484 ,  G01N33/4833 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2015/1493 ,  G02B21/32

Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.