Abstract:
PROBLEM TO BE SOLVED: To provide novel methods for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles.SOLUTION: The invention includes apparatus, methods, and kits for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention includes apparatus, methods, and kits for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order, and employed for any suitable number of times within a system.
Abstract:
PROBLEM TO BE SOLVED: To provide a new method for microfluidic manipulation and/or analysis of particles (e.g., cells, viruses, organelles, beads, and/or vesicles). SOLUTION: A microfluidic particle-analysis system includes a device, a method, and a kit for the microfluidic manipulation and/or detection of the particles such as cells and/or beads. The system includes the device, the method and the kit for the microfluidic manipulation and/or analysis of the particles such as the cells, viruses, organelles, beads and/or vesicles, and microfluidic mechanisms are provided for performing such the manipulations and analyses. Such mechanisms enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of the particles. Further, these mechanisms can be combined in any suitable order and employed in any suitable number of times within the system. COPYRIGHT: (C)2010,JPO&INPIT
Abstract:
PROBLEM TO BE SOLVED: To provide devices that can be utilized to conduct a variety of nucleic acid amplification reactions, while having sufficient versatility for use in other types of analyses as well.SOLUTION: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Some devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
Abstract:
PROBLEM TO BE SOLVED: To provide a device which can be utilized to conduct a variety of nucleic acid amplification reactions, while having sufficient versatility for use in other types of analyses as well. SOLUTION: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping, and gene expression analyses. COPYRIGHT: (C)2011,JPO&INPIT
Abstract:
A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies.
Abstract:
A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
Abstract:
High throughput screening of crystallization of a target material is accomplished by simultaneously introducing a solution of the target material into a plurality of chambers of a microfabricated fluidic device (4200). The microfabricated fluidic device is then manipulated to vary the solution condition in the chambers (4202a, 4202b), thereby simultaneously providing a large number of crystallization environments. Control over changed solution conditions may result from a variety of techniques, including but not limited to metering volumes of crystallizing agent into the chamber by volume exclusion, by entrapment of volumes of crystallizing agent determined by the dimensions of the microfabricated structure, or by cross-channel injection of sample and crystallizing agent into an array of junctions defined by intersecting orthogonal flow channels.
Abstract:
Introducing a sample solution that includes a target nucleic acid polymer into a microfluidic device that has a sample region with: (i) a flow channel in fluid communication with one fluid inlet and one fluid outlet; and (ii) a plurality of valves that, when closed, partition the flow channel into isolated reaction sites. Each valve is formed from an elastomeric membrane that separates a control channel from the flow channel and which is disposed to be deflected into or retracted out from the flow channel in response to an actuation force to the control channel, thereby forming the valve. The method includes closing the valves, thereby partitioning the flow channel into isolated reaction sites containing portions of said sample solution; exposing the reaction sites to conditions under which the nucleic acid polymer is amplified to produce an amplification product; and opening said valves and displacing sample solution out of the flow channel.