公开(公告)号：EP3201599A1

公开(公告)日：2017-08-09

申请号：EP15771133.4

申请日：2015-09-30

Applicant: FOSS Analytical A/S

Inventor： RINDORF, Lars Henning ,  GLÜCKSTAD, Jesper ,  AABO, Thomas

IPC: G01N15/14 ,  B01L3/00 ,  G01N15/10 ,  G01N15/00

CPC classification number: G01N15/1404 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2400/0454 ,  B01L2400/082 ,  G01N15/147 ,  G01N15/1484 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1413 ,  G01N2015/1415 ,  G01N2015/149

Abstract: In a method for hydrodynamic focusing of a laminar and planar sample fluid flow, a system is provided for analysis and/or sorting of microscopic objects in the sample fluid comprising an optical objective for optical inspection of the microscopic objects. Microscopic objects are conveyed in the laminar flow of the sample fluid, and two laminar and planar flow of sheath fluids are provided. The flow of the sample fluid is hydrodynamically focussed at an optical inspection zone of the system by the sheath fluids. Focussing of the flow of the sample fluid is controlled such that all of the microscopic objects in the sample fluid are caused to be conveyed in a common flow direction in one single plane at the inspection zone of the system, and the microscopic objects in the fluid are optically inspected through the optical objective.

Abstract translation: 在用于层流和平面样品流体流动的流体动力聚焦的方法中，提供了用于分析和/或分选样品流体中的微观物体的系统，其包括用于光学检查微观物体的光学物镜。 微观物体在样品流体的层流中传送，并提供两层层流和平面流体的鞘液。 样品流体的流动通过鞘液在流体动力学聚焦在系统的光学检查区。 控制样本流体的流动，使得样本流体中的所有微观物体在系统的检查区域中在一个单一平面内以共同流动方向被传送，并且流体中的微观物体 通过光学物镜进行光学检查。

公开(公告)号：WO2016050837A1

公开(公告)日：2016-04-07

申请号：PCT/EP2015/072545

申请日：2015-09-30

Applicant: FOSS ANALYTICAL A/S

Inventor： RINDORF, Lars Henning ,  GLÜCKSTAD, Jesper ,  AABO, Thomas

IPC: G01N15/14 ,  B01L3/00 ,  G01N15/10 ,  G01N15/00

CPC classification number: G01N15/1404 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2400/0454 ,  B01L2400/082 ,  G01N15/147 ,  G01N15/1484 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1413 ,  G01N2015/1415 ,  G01N2015/149

Abstract: In a method for hydrodynamic focusing of a laminar and planar sample fluid flow, a system is provided for analysis and/or sorting of microscopic objects in the sample fluid comprising an optical objective for optical inspection of the microscopic objects. Microscopic objects are conveyed in the laminar flow of the sample fluid, and two laminar and planar flow of sheath fluids are provided. The flow of the sample fluid is hydrodynamically focussed at an optical inspection zone of the system by the sheath fluids. Focussing of the flow of the sample fluid is controlled such that all of the microscopic objects in the sample fluid are caused to be conveyed in a common flow direction in one single plane at the inspection zone of the system, and the microscopic objects in the fluid are optically inspected through the optical objective.

Abstract translation: 在层流和平面样品流体流动的流体动力学聚焦的方法中，提供用于分析和/或分类样品流体中的微观物体的系统，其包括用于光学检查微观物体的光学物镜。 微观物体在样品流体的层流中传送，并且提供两个层流和平面的护套流体流。 样品流体的流动通过鞘液在流体动力学上集中在系统的光学检查区域。 控制样品流动的聚焦，使得样品流体中的所有微观物体在系统的检查区域的一个单一平面中沿共同的流动方向被输送，并且流体中的微观物体 通过光学物镜进行光学检查。