公开(公告)号：WO2018022776A1

公开(公告)日：2018-02-01

申请号：PCT/US2017/043989

申请日：2017-07-26

Applicant: IMRA AMERICA, INC.

Inventor： MILLS, Andrew, A. ,  CEDERQUIST, Kristin, B. ,  LIU, Bing

IPC: C08G65/328 ,  G01N33/20

Abstract: Disclosed is a method of pulsed laser ablation for producing gold-platinum AuxPti-x alloy nanoparticles in a colloidal solution. The resulting colloidal solution of AuxPti-x alloy nanoparticles is suitable for a variety of biological applications including lateral flow immunoassays and other bio-detections based on optical scattering of metal nanoparticles. In the present method, the laser pulse duration is kept in the picosecond to femtosecond region, which is sufficiently short to ablate the target without significantly heating the target material. The nanoparticles form by fragmentation of the bulk material without evaporation, minimizing oxidation of the nanoparticles. The nanoparticles conjugate with bio-molecules such as protein, antibodies, peptides, RNA oligomers, DNA oligomers, other oligomers, or polymers effectively by passive adsorption. This process of bio-molecule conjugation is the same as that with pure gold nanoparticles, and will not require significant changes in the fabrication protocols for bioassay and bio-detection device manufacturers. Advantageously the AuxPti-x alloy nanoparticles have a wide optical extinction spectrum in the visible region, appearing nearly black in both colloidal and dried form. The nanoparticles can be used for labeling bio-molecules and provide a high visual contrast in visual-based bioassays such as lateral flow immunoassays against the white test paper strips. A combination of the near black color of the AuxPti-x alloy nanoparticles with the red color of pure Au nanoparticles makes multiplexing bio-detection assays possible.

Abstract translation: 公开了一种用于在胶体溶液中产生金 - 铂AuxPti-x合金纳米颗粒的脉冲激光烧蚀方法。 得到的AuxPti-x合金纳米颗粒胶体溶液适用于各种生物应用，包括横向流动免疫分析和基于金属纳米颗粒光散射的其他生物检测。 在本方法中，激光脉冲持续时间保持在皮秒至飞秒区域，该区域足够短以消融目标而不显着加热目标材料。 纳米颗粒通过大块材料的碎裂形成而不蒸发，从而使纳米颗粒的氧化最小化。 纳米粒子与生物分子如蛋白质，抗体，肽，RNA寡聚物，DNA寡聚物，其他寡聚物或聚合物通过被动吸附有效地结合。 这种生物分子结合过程与纯金纳米粒子结合的过程相同，并且不需要对生物测定和生物检测设备制造商的制造方案进行重大改变。 有利地，AuxPti-x合金纳米颗粒在可见光区具有宽的消光光谱，在胶体和干燥形式下都呈现接近黑色。 纳米粒子可用于标记生物分子，并在基于视觉的生物分析中提供高度的视觉对比度，例如针对白色试纸条的横向流动免疫分析。 AuxPti-x合金纳米粒子的接近黑色与纯金纳米粒子的红色组合使得多重生物检测分析成为可能。