公开(公告)号：WO2018022776A1

公开(公告)日：2018-02-01

申请号：PCT/US2017/043989

申请日：2017-07-26

Applicant: IMRA AMERICA, INC.

Inventor： MILLS, Andrew, A. ,  CEDERQUIST, Kristin, B. ,  LIU, Bing

IPC: C08G65/328 ,  G01N33/20

Abstract: Disclosed is a method of pulsed laser ablation for producing gold-platinum AuxPti-x alloy nanoparticles in a colloidal solution. The resulting colloidal solution of AuxPti-x alloy nanoparticles is suitable for a variety of biological applications including lateral flow immunoassays and other bio-detections based on optical scattering of metal nanoparticles. In the present method, the laser pulse duration is kept in the picosecond to femtosecond region, which is sufficiently short to ablate the target without significantly heating the target material. The nanoparticles form by fragmentation of the bulk material without evaporation, minimizing oxidation of the nanoparticles. The nanoparticles conjugate with bio-molecules such as protein, antibodies, peptides, RNA oligomers, DNA oligomers, other oligomers, or polymers effectively by passive adsorption. This process of bio-molecule conjugation is the same as that with pure gold nanoparticles, and will not require significant changes in the fabrication protocols for bioassay and bio-detection device manufacturers. Advantageously the AuxPti-x alloy nanoparticles have a wide optical extinction spectrum in the visible region, appearing nearly black in both colloidal and dried form. The nanoparticles can be used for labeling bio-molecules and provide a high visual contrast in visual-based bioassays such as lateral flow immunoassays against the white test paper strips. A combination of the near black color of the AuxPti-x alloy nanoparticles with the red color of pure Au nanoparticles makes multiplexing bio-detection assays possible.

Abstract translation: 公开了一种用于在胶体溶液中产生金 - 铂AuxPti-x合金纳米颗粒的脉冲激光烧蚀方法。 得到的AuxPti-x合金纳米颗粒胶体溶液适用于各种生物应用，包括横向流动免疫分析和基于金属纳米颗粒光散射的其他生物检测。 在本方法中，激光脉冲持续时间保持在皮秒至飞秒区域，该区域足够短以消融目标而不显着加热目标材料。 纳米颗粒通过大块材料的碎裂形成而不蒸发，从而使纳米颗粒的氧化最小化。 纳米粒子与生物分子如蛋白质，抗体，肽，RNA寡聚物，DNA寡聚物，其他寡聚物或聚合物通过被动吸附有效地结合。 这种生物分子结合过程与纯金纳米粒子结合的过程相同，并且不需要对生物测定和生物检测设备制造商的制造方案进行重大改变。 有利地，AuxPti-x合金纳米颗粒在可见光区具有宽的消光光谱，在胶体和干燥形式下都呈现接近黑色。 纳米粒子可用于标记生物分子，并在基于视觉的生物分析中提供高度的视觉对比度，例如针对白色试纸条的横向流动免疫分析。 AuxPti-x合金纳米粒子的接近黑色与纯金纳米粒子的红色组合使得多重生物检测分析成为可能。

公开(公告)号：WO2015183659A1

公开(公告)日：2015-12-03

申请号：PCT/US2015/031731

申请日：2015-05-20

Applicant: IMRA AMERICA, INC.

Inventor： QIAN, Wei ,  LIU, Bing ,  FETTERS, Kori, Michael

IPC: G01N33/58 ,  C12Q1/37

CPC classification number: C12Q1/37 ,  G01N33/587 ,  G01N2333/81 ,  G01N2333/948

Abstract: In the present invention, a method and assay for the detection of proteases and protease inhibitors using colloidal gold nanoparticles and peptide substrates, which are selectively recognized and cleaved by proteases being assayed, is disclosed. In this assay, the mechanism of signal generation relies on peptide sequence induced aggregation of gold nanoparticles, which are used as signal reporters. The peptide sequences that induce aggregation are either the intact peptide substrates or proteolytic fragments of the intact peptide substrate wherein the proteolytic fragments are produced by the protease being assayed. The present invention provides a novel, simple, sensitive, and inexpensive colloidal gold nanoparticle-based colorimetric assay that allows both visual and quantitative detection of proteases and protease inhibitors.

Abstract translation: 在本发明中，公开了使用被测定蛋白酶选择性识别和切割的胶体金纳米颗粒和肽底物检测蛋白酶和蛋白酶抑制剂的方法和测定法。 在该测定中，信号产生的机制依赖于用作信号记录器的金纳米颗粒的肽序列诱导的聚集。 诱导聚集的肽序列是完整肽底物的完整肽底物或蛋白水解片段，其中蛋白水解片段由待测蛋白酶产生。 本发明提供了一种新颖，简单，灵敏和便宜的基于胶体金纳米颗粒的比色测定法，其允许视觉和定量检测蛋白酶和蛋白酶抑制剂。

公开(公告)号：WO2011100152A1

公开(公告)日：2011-08-18

申请号：PCT/US2011/023527

申请日：2011-02-03

Applicant: IMRA AMERICA, INC. ,  LIU, Bing ,  CHE, Yong

Inventor： LIU, Bing ,  CHE, Yong

IPC: B32B9/04 ,  B82B3/00

CPC classification number: H01L31/035281 ,  B22F1/0022 ,  B22F9/04 ,  B22F2998/00 ,  B22F2999/00 ,  B23K26/361 ,  B82B1/001 ,  B82Y40/00 ,  H01L31/0296 ,  H01L31/0322 ,  H01L31/03923 ,  H01L31/03925 ,  H01L31/1828 ,  Y02E10/541 ,  Y02E10/543 ,  Y02P70/521 ,  B22F1/0018 ,  B22F2202/11

Abstract: A method of producing nanoparticles of solar light absorbing compound materials based on pulsed laser ablation is disclosed. The method uses irradiation of a target material of solar light absorbing compound material with a pulsed laser beam having a pulse duration of from 10 femtoseconds to 500 picoseconds to ablate the target thereby producing nanoparticles of the target The nanoparticles are collected and a solution of the nanoparticles is applied to a substrate to produce a thin film solar cell. The method preserves the composition and structural crystalline phase of the starting target. The method is a much lower cost fabrication method for thin film solar cells.

Abstract translation: 公开了一种基于脉冲激光烧蚀制备太阳光吸收复合材料的纳米颗粒的方法。 该方法使用具有10飞秒至500皮秒的脉冲持续时间的脉冲激光束照射太阳光吸收复合材料的目标材料以烧蚀靶，从而产生靶的纳米颗粒。收集纳米颗粒，并将纳米颗粒的溶液 施加到基板上以制造薄膜太阳能电池。 该方法保留起始靶的组成和结构结晶相。 该方法是薄膜太阳能电池成本低廉的制造方法。

公开(公告)号：WO2013063090A2

公开(公告)日：2013-05-02

申请号：PCT/US2012/061656

申请日：2012-10-24

Applicant: IMRA AMERICA, INC.

Inventor： HAGEDORN, Kevin, V. ,  LIU, Bing

IPC: C25D13/02

CPC classification number: C25D13/02 ,  B82Y30/00 ,  C25D13/10 ,  Y10T428/25 ,  Y10T428/31678

Abstract: Disclosed is a process for electrophoretic deposition of colloidal suspensions of nanoparticles, especially from aprotic solvents, onto a variety of substrates. The process provides chemical additives that can be used to improve thin films deposited from colloidal suspensions by increasing the rate of deposition and the smoothness of the deposited film. In this process, a chemical additive is used to improve the properties of the deposited thin films. The chemical additive comprises a redox couple, an organometallic complex, a metallocene, a ferrocene, or a nickelocene. The colloidal suspension can be composed of semiconductor, metal or ceramic nanoparticles suspended in an aprotic polar solvent such as acetone, acetonitrile, or pyridine. The process also improves the properties of thin films deposited from protic solvents. The particles have at least one dimension ranging from 0.1 nanometers (nm) to 500 nm.

Abstract translation: 公开了一种将纳米粒子，特别是非质子溶剂的胶体悬浮液电泳沉积到各种基底上的方法。 该方法提供化学添加剂，其可用于通过增加沉积速率和沉积膜的平滑度来改善由胶体悬浮液沉积的薄膜。 在该方法中，使用化学添加剂来改善沉积的薄膜的性质。 化学添加剂包括氧化还原对，有机金属络合物，茂金属，二茂铁或二茂镍。 胶态悬浮液可以由悬浮在非质子极性溶剂如丙酮，乙腈或吡啶中的半导体，金属或陶瓷纳米颗粒组成。 该方法还改善了由质子溶剂沉积的薄膜的性质。 颗粒具有0.1纳米（nm）至500nm的至少一个维度。

公开(公告)号：WO2009148674A1

公开(公告)日：2009-12-10

申请号：PCT/US2009/036789

申请日：2009-03-11

Applicant: IMRA AMERICA, INC. ,  MURAKAMI, Makoto ,  HU, Zhendong ,  LIU, Bing ,  CHE, Yong ,  LIU, Zhenlin ,  UEHARA, Yuzuru

Inventor： MURAKAMI, Makoto ,  HU, Zhendong ,  LIU, Bing ,  CHE, Yong ,  LIU, Zhenlin ,  UEHARA, Yuzuru

IPC: H01L21/44

CPC classification number: C23C14/28 ,  C23C14/08 ,  C23C14/564 ,  H01S3/0057 ,  H01S3/0085 ,  H01S3/2308

Abstract: A method of pulsed laser deposition (PLD) capable of continuously tuning formed-film morphology from that of a nanoparticle aggregate to a smooth thin film free of particles and droplets. The materials that can be synthesized using various embodiments of the invention include, but are not limited to, metals, alloys, metal oxides, and semiconductors. In various embodiments a 'burst' mode of ultrashort pulsed laser ablation and deposition is provided. Tuning of the film morphology is achieved by controlling the burst-mode parameters such as the number of pulses and the time-spacing between the pulses within each burst, the burst repetition rate, and the laser fluence. The system includes an ultrashort pulsed laser, an optical system for delivering a focused onto the target surface with an appropriate energy density, and a vacuum chamber in which the target and the substrate are installed and background gases and their pressures are appropriately adjusted.

Abstract translation: 脉冲激光沉积（PLD）的方法，其能够将形成膜的形态从纳米颗粒聚集体的形态连续调节到没有颗粒和液滴的平滑薄膜。 可以使用本发明的各种实施方案合成的材料包括但不限于金属，合金，金属氧化物和半导体。 在各种实施例中，提供了超短脉冲激光烧蚀和沉积的“突发”模式。 通过控制脉冲串模式参数，例如脉冲数和每个脉冲串内的脉冲之间的时间间隔，脉冲串重复频率和激光能量密度来实现电影形态的调整。 该系统包括超短脉冲激光器，用于以适当的能量密度传递聚焦到目标表面上的光学系统，以及真空室，其中靶和基底被安装在其中，背景气体及其压力被适当调节。

公开(公告)号：WO2012061023A1

公开(公告)日：2012-05-10

申请号：PCT/US2011/057000

申请日：2011-10-20

Applicant: IMRA AMERICA, INC. ,  GUO, Wei ,  JIN, Yu ,  LIU, Bing ,  CHE, Yong ,  HAGEDORN, Kevin, V.

Inventor： GUO, Wei ,  JIN, Yu ,  LIU, Bing ,  CHE, Yong ,  HAGEDORN, Kevin, V.

IPC: H01L21/02 ,  H01L21/368 ,  H01L31/18

CPC classification number: H01L21/02628 ,  H01L21/02568 ,  H01L21/02601 ,  H01L21/02614 ,  H01L31/0322 ,  H01L31/0749 ,  Y02E10/541

Abstract: The present invention provides a non-vacuum method of depositing a photovoltaic absorber layer based on electrophoretic deposition of a mixture of nanoparticles with a controlled atomic ratio between the elements. The nanoparticles are first dispersed in a liquid medium to form a colloidal suspension and then electrophoretically deposited onto a substrate to form a thin film photovoltaic absorber layer. The absorber layer may be subjected to optional post-deposition treatments for photovoltaic absorption.

Abstract translation: 本发明提供了一种非真空方法，其基于在元件之间具有受控原子比的纳米颗粒的混合物的电泳沉积来沉积光伏吸收层。 首先将纳米颗粒分散在液体介质中以形成胶体悬浮液，然后电泳沉积到基底上以形成薄膜光伏吸收层。 可以对吸收层进行用于光伏吸收的任选的后沉积处理。

公开(公告)号：WO2011100153A1

公开(公告)日：2011-08-18

申请号：PCT/US2011/023529

申请日：2011-02-03

Applicant: IMRA AMERICA, INC. ,  MURAKAMI, Makoto ,  CHE, Yong ,  LIU, Bing ,  ICHIKAWA, Yuki

Inventor： MURAKAMI, Makoto ,  CHE, Yong ,  LIU, Bing ,  ICHIKAWA, Yuki

IPC: G01J3/44 ,  B32B3/10

CPC classification number: G01N21/658 ,  B82Y30/00 ,  B82Y40/00 ,  C23C14/083 ,  C23C14/3435

Abstract: An device for Raman spectroscopy such as surface enhanced Raman spectroscopy (SERS) is disclosed herein. Various embodiments may be utilized to prepare a SERS substrate using several deposition techniques such as pulsed laser deposition. Some embodiments optimize coverage, volume, or elements of SERS active metals. The method is a single step inexpensive method for preparing a SERS active substrate. In some embodiments a coating layer underneath the SERS active metals is utilized for additional enhancements.

Abstract translation: 本文公开了一种用于拉曼光谱的装置，例如表面增强拉曼光谱（SERS）。 可以使用多种沉积技术例如脉冲激光沉积来制备SERS衬底的各种实施例。 一些实施例优化SERS活性金属的覆盖，体积或元素。 该方法是用于制备SERS活性基底的单步廉价方法。 在一些实施方案中，SERS活性金属下面的涂层用于额外的增强。

公开(公告)号：WO2014066138A1

公开(公告)日：2014-05-01

申请号：PCT/US2013/065456

申请日：2013-10-17

Applicant: IMRA AMERICA, INC.

Inventor： LIU, Bing ,  ICHIKAWA, Yuki

IPC: C21D1/09 ,  B05D3/00

CPC classification number: B23K26/0084 ,  B05D3/06 ,  B05D5/08 ,  B05D5/083 ,  B05D2451/00 ,  B23K26/009 ,  C21D1/09 ,  C21D10/00 ,  C21D2201/00 ,  Y10T428/24355 ,  B05D2401/32 ,  B05D2401/40

Abstract: A method of pulsed laser processing of solid surface for enhancing surface hydrophobicity is disclosed wherein the solid surface is covered with a transparent medium during laser processing and the laser beam incidents through the covering medium and irradiates the solid surface. Two effects are obtained simultaneously. One is the laser-induced texture formation directly under the laser irradiation. The other is the deposition of the laser-removed materials along the laser scan lines. Both effects introduce surface roughness on nanometer scales, and both enhance surface hydrophobicity, rendering superhydrophobicity on the surfaces of both the laser-irradiated solid and the covering medium. Because the beam scan line spacing can be larger than a single scan line width by multiple times, this method provides a high processing speed of square inch per minute and enables large area processing.

Abstract translation: 公开了一种用于提高表面疏水性的固体表面的脉冲激光加工方法，其中固体表面在激光加工期间被透明介质覆盖，并且激光束通过覆盖介质入射并照射固体表面。 同时获得两个效果。 一种是直接在激光照射下的激光诱导纹理形成。 另一种是激光去除材料沿着激光扫描线的沉积。 这两种效应都会在纳米尺度上引入表面粗糙度，同时增强表面疏水性，从而在激光照射的固体和覆盖介质的表面上产生超疏水性。 由于光束扫描线间距可以大于单个扫描线宽度多次，因此该方法提供了高达每分钟平方英寸的处理速度，可实现大面积处理。

公开(公告)号：WO2013085825A1

公开(公告)日：2013-06-13

申请号：PCT/US2012/067512

申请日：2012-12-03

Applicant: IMRA AMERICA INC.

Inventor： HAGEDORN, Kevin, V. ,  LIU, Bing ,  CHE, Yong

IPC: G06F19/00

CPC classification number: B01J37/349 ,  B01J21/185 ,  B01J23/62 ,  B01J23/626 ,  B01J23/628 ,  B01J23/6447 ,  B01J23/6525 ,  B01J23/892 ,  B01J35/0013 ,  B01J35/002 ,  B01J35/1014 ,  B01J37/344 ,  B82Y30/00 ,  B82Y40/00 ,  H01M4/921 ,  Y10S977/773 ,  Y10S977/81 ,  Y10S977/889

Abstract: At least one embodiment includes a method for fabricating a catalyst comprising a colloidal suspension of nanoparticles, the nanoparticles comprising intermetallics of two or more metals exhibiting long range superlattice crystal ordering. The method comprising the steps of: producing a bulk target of the intermetallics of two or more metals exhibiting long range crystal ordering and submerging the target in a solvent. A pulsed laser is used to ablate bulk target material and to produce nanoparticle of the intermetallics of two or more metals exhibiting long range crystal ordering. At least one embodiment includes a catalyst made with the method. The catalyst can exhibit some desirable properties. For example, the catalyst may remain suspended in solution, essentially without surface modification by ionic compounds. Furthermore, the concentration of elements other than those which comprise the solvent or the intermetallic compound may be less than about 1 ppm.

Abstract translation: 至少一个实施方案包括用于制造包含纳米颗粒的胶态悬浮液的催化剂的方法，所述纳米颗粒包含两种或更多种呈现长范围超晶格晶体排列的金属的金属间化合物。 该方法包括以下步骤：制备两种或多种金属间化合物的体积目标物，其显示长程晶体排序并将靶浸入溶剂中。 使用脉冲激光来消融体目标材料并产生呈现长程晶体排列的两种或多种金属的金属间化合物的纳米颗粒。 至少一个实施方案包括用该方法制备的催化剂。 催化剂可以表现出一些期望的性质。 例如，催化剂可以保持悬浮在溶液中，基本上没有通过离子化合物的表面改性。 此外，除了包含溶剂或金属间化合物的元素之外的元素的浓度可以小于约1ppm。

公开(公告)号：WO2012134853A1

公开(公告)日：2012-10-04

申请号：PCT/US2012/029623

申请日：2012-03-19

Applicant: IMRA AMERICA, INC. ,  LIU, Bing ,  QIAN, Wei ,  MURAKAMI, Makoto ,  CHE, Yong

Inventor： LIU, Bing ,  QIAN, Wei ,  MURAKAMI, Makoto ,  CHE, Yong

IPC: G01J3/44

CPC classification number: B05D3/06 ,  B05D7/22 ,  B82Y30/00 ,  B82Y40/00 ,  G01J3/44 ,  G01N21/658 ,  G01N2021/651

Abstract: An apparatus for performing surface-enhanced Raman scattering (SERS) is disclosed wherein an inner surface of a container is coated with SERS active materials such as nanoparticles of noble metals. Such a container can provide a partially enclosed, optical diffuse cavity whose inner surfaces serve for dual purposes of enhancing Raman scattering of the contained analyte and optical integration, therefore improving the efficiency of optical excitation and signal collection. The container may be configured to isolate the SERS active material from the external environment. The container, which may be a cylindrical tube, may be referred to as a SERS tube. Methods of coating the inner wall of a container with pulsed laser ablation and with nanoparticle colloids, respectively, are disclosed.

Abstract translation: 公开了一种用于进行表面增强拉曼散射（SERS）的设备，其中容器的内表面涂覆有SERS活性材料，例如贵金属的纳米颗粒。 这种容器可以提供部分封闭的光学漫射腔，其内表面用于增强所包含的分析物的拉曼散射和光学积分的双重目的，从而提高光学激发和信号采集的效率。 容器可以被配置为将SERS活性材料与外部环境隔离。 可以将圆筒形管的容器称为SERS管。 公开了分别用脉冲激光烧蚀和纳米颗粒胶体涂覆容器内壁的方法。