公开(公告)号：EP3222728A1

公开(公告)日：2017-09-27

申请号：EP15861198.8

申请日：2015-11-19

Applicant: Institute for Basic Science

Inventor： KIM, Jin Soo ,  KOO, Tae Young

IPC: C12N15/63 ,  C12N9/16

CPC classification number: C12N15/746 ,  C12N9/16 ,  C12N9/22 ,  C12N15/11 ,  C12N15/63 ,  C12N15/8645 ,  C12N15/867

Abstract: The present invention relates to a method for regulating gene expression, comprising introducing into a cell each of a recombinant vector which expresses a first domain comprising N-terminus of a Cas9 protein, and a recombinant vector which expresses a second domain comprising C-terminus of a Cas9 protein, a composition comprising the recombinant vectors, a kit for regulating gene expression, and a method for intracellular production of Cas9 protein. Moreover, the present invention relates to a transformed cell introduced with a viral vector which packages the first domain, and a viral vector which packages the second domain, and to a composition comprising a virus produced therefrom.

Abstract translation: 本发明涉及调节基因表达的方法，包括向细胞中导入表达包含Cas9蛋白的N端的第一结构域的重组载体和表达第二结构域的重组载体，所述第二结构域包含 Cas9蛋白质，包含该重组载体的组合物，用于调节基因表达的试剂盒以及用于细胞内产生Cas9蛋白质的方法。 此外，本发明涉及导入了包装第一结构域的病毒载体和包装第二结构域的病毒载体的转化细胞，以及包含由其产生的病毒的组合物。