公开(公告)号：US10253378B2

公开(公告)日：2019-04-09

申请号：US15037311

申请日：2014-11-18

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Inchul Yang ,  Hannah Yu ,  Hyo-jin Yang ,  Sook-Kyung Kim ,  Sang-Ryoul Park

IPC: C12Q1/68 ,  C12Q1/689

Abstract: The present invention relates to utilization of an artificially synthesized nucleic acid, and more particularly, to a quantitative analysis method capable of quantitatively adjusting gene-based microbial community analysis results by preparing a microorganism 16S rDNA gene, which has a single nucleotide polymorphism (SNP) at a particular location so as to be differentiated from a gene of a target microorganism on the nucleotide sequence, and then using the microorganism 16S rDNA gene as an internal standard material which is quantifiable through nucleotide sequencing.

公开(公告)号：US20160376638A1

公开(公告)日：2016-12-29

申请号：US15037311

申请日：2014-11-18

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Inchul Yang ,  Hannah Yu ,  Hyo-jin Yang ,  Sook-Kyung Kim ,  Sang-Ryoul Park

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The present invention relates to utilization of an artificially synthesized nucleic acid, and more particularly, to a quantitative analysis method capable of quantitatively adjusting gene-based microbial community analysis results by preparing a microorganism 16S rDNA gene, which has a single nucleotide polymorphism (SNP) at a particular location so as to be differentiated from a gene of a target microorganism on the nucleotide sequence, and then using the microorganism 16S rDNA gene as an internal standard material which is quantifiable through nucleotide sequencing.

Abstract translation: 本发明涉及人工合成的核酸的利用，更具体地说，涉及一种能够通过制备具有单核苷酸多态性（SNP）的微生物16S rDNA基因来定量调整基因的微生物群落分析结果的定量分析方法， 在特定位置，以便与核苷酸序列上的靶微生物基因区别，然后使用微生物16S rDNA基因作为可通过核苷酸测序定量的内标物质。