公开(公告)号：US20150210746A1

公开(公告)日：2015-07-30

申请号：US14657228

申请日：2015-03-13

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Jun-Hyuk Choi ,  Sook-Kyung Kim ,  Minji Kim

IPC: C07K14/61 ,  B01D15/42 ,  B01D15/34 ,  B01D15/38 ,  B01D15/36

CPC classification number: C07K14/61 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3823 ,  B01D15/426

Abstract: The present invention relates to a method for expressing, extracting and refining recombinant human growth hormone (hGH). More particularly, the present invention relates to a method for the intracellular expression of a target protein with minimizing the formation of insoluble inclusion bodies during the mass production of the protein in Escherichia coli system. The present invention also relates to a method for extracting and refining the target protein, particularly human growth hormone, with maximizing its solubility during the extraction and with providing a high yield but without losing the biological activity of the target protein.

Abstract translation: 本发明涉及用于表达，提取和精制重组人生长激素（hGH）的方法。 更具体地说，本发明涉及一种在大肠杆菌系统中大量生产蛋白质期间最小化不溶性包涵体形成的靶蛋白的细胞内表达的方法。 本发明还涉及一种用于提取和精制目标蛋白质，特别是人生长激素的方法，其在提取期间最大限度地提高其溶解度并提供高产率但不丧失靶蛋白的生物活性。

公开(公告)号：US10253378B2

公开(公告)日：2019-04-09

申请号：US15037311

申请日：2014-11-18

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Inchul Yang ,  Hannah Yu ,  Hyo-jin Yang ,  Sook-Kyung Kim ,  Sang-Ryoul Park

IPC: C12Q1/68 ,  C12Q1/689

Abstract: The present invention relates to utilization of an artificially synthesized nucleic acid, and more particularly, to a quantitative analysis method capable of quantitatively adjusting gene-based microbial community analysis results by preparing a microorganism 16S rDNA gene, which has a single nucleotide polymorphism (SNP) at a particular location so as to be differentiated from a gene of a target microorganism on the nucleotide sequence, and then using the microorganism 16S rDNA gene as an internal standard material which is quantifiable through nucleotide sequencing.

公开(公告)号：US20140302545A1

公开(公告)日：2014-10-09

申请号：US14345835

申请日：2012-09-18

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Duk Jin Kang ,  Sang Ryoul Park ,  Sook-Kyung Kim

IPC: C12Q1/37

CPC classification number: C12Q1/37 ,  C07K1/20 ,  C07K1/34 ,  C07K1/36 ,  C12M21/18 ,  C12M25/12 ,  C12P21/06

Abstract: Provided are an enzyme treatment apparatus for proteins using a hollow fiber membrane and an on-line proteomics method using same. The enzyme treatment apparatus for proteins according to the present invention can increase a recovery rate of peptides, which are recovered through an enzyme treatment process, by basically solving the problem of low reproducibility of an enzyme activity which may occur during a conventional enzyme treatment process, and can also reduce a time for purification and provide higher yield by performing separation and purification through a single step. In particular, the present invention can be usefully applied for developing a disease-specific protein biomarker through a statistical analysis method having highly efficient detectability for proteins in research for finding biomarkers related to human diseases.

Abstract translation: 提供了使用中空纤维膜的蛋白质的酶处理装置和使用其的在线蛋白质组学方法。 根据本发明的蛋白质的酶处理装置可以通过基本上解决在常规酶处理过程中可能发生的酶活性的低再现性的问题来提高通过酶处理过程回收的肽的回收率， 并且还可以通过单步执行分离和纯化来减少纯化时间并提供更高的产率。 特别地，本发明可以有用地用于通过统计分析方法开发疾病特异性蛋白质生物标志物，所述统计分析方法在用于寻找与人类疾病相关的生物标志物的研究中具有高度可检测性的蛋白质。

公开(公告)号：US09598717B2

公开(公告)日：2017-03-21

申请号：US14345835

申请日：2012-09-18

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Duk Jin Kang ,  Sang Ryoul Park ,  Sook-Kyung Kim

IPC: C12Q1/37 ,  C07K1/34 ,  C12M1/40 ,  C12M1/12 ,  C12P21/06 ,  C07K1/36 ,  C07K1/20

CPC classification number: C12Q1/37 ,  C07K1/20 ,  C07K1/34 ,  C07K1/36 ,  C12M21/18 ,  C12M25/12 ,  C12P21/06

Abstract: Provided are an enzyme treatment apparatus for proteins using a hollow fiber membrane and an on-line proteomics method using same. The enzyme treatment apparatus for proteins according to the present invention can increase a recovery rate of peptides, which are recovered through an enzyme treatment process, by basically solving the problem of low reproducibility of an enzyme activity which may occur during a conventional enzyme treatment process, and can also reduce a time for purification and provide higher yield by performing separation and purification through a single step. In particular, the present invention can be usefully applied for developing a disease-specific protein biomarker through a statistical analysis method having highly efficient detectability for proteins in research for finding biomarkers related to human diseases.

公开(公告)号：US20160376638A1

公开(公告)日：2016-12-29

申请号：US15037311

申请日：2014-11-18

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Inchul Yang ,  Hannah Yu ,  Hyo-jin Yang ,  Sook-Kyung Kim ,  Sang-Ryoul Park

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The present invention relates to utilization of an artificially synthesized nucleic acid, and more particularly, to a quantitative analysis method capable of quantitatively adjusting gene-based microbial community analysis results by preparing a microorganism 16S rDNA gene, which has a single nucleotide polymorphism (SNP) at a particular location so as to be differentiated from a gene of a target microorganism on the nucleotide sequence, and then using the microorganism 16S rDNA gene as an internal standard material which is quantifiable through nucleotide sequencing.

Abstract translation: 本发明涉及人工合成的核酸的利用，更具体地说，涉及一种能够通过制备具有单核苷酸多态性（SNP）的微生物16S rDNA基因来定量调整基因的微生物群落分析结果的定量分析方法， 在特定位置，以便与核苷酸序列上的靶微生物基因区别，然后使用微生物16S rDNA基因作为可通过核苷酸测序定量的内标物质。