公开(公告)号：US20240228785A1

公开(公告)日：2024-07-11

申请号：US18419897

申请日：2024-01-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen HU ,  Aimei CHEN ,  Daniel BEACHAM ,  Chrisgen VONNEGUT ,  Krishnamurthy NACHARAJU

IPC: C09B11/24 ,  G01N21/64

CPC classification number: C09B11/24 ,  G01N21/6428 ,  G01N2021/6439

Abstract: The present disclosure provides for compounds of Formula (I),




its corresponding compounds of Formula (II)




or salts thereof and their use as fluorogenic pH sensors.

公开(公告)号：US20230313272A1

公开(公告)日：2023-10-05

申请号：US18179184

申请日：2023-03-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chrisgen VONNEGUT ,  Scott CLARKE

IPC: C12Q1/6809

CPC classification number: C12Q1/6809 ,  C12Q2563/107 ,  C12Q2565/102

Abstract: Disclosed is a method for determining the quality, expressed in terms of a quality value, of an RNA sample based on a multiplex fluorescent ratiometric method, wherein the ratio of small RNA to large and/or structured RNA in a sample is determined, thereby obtaining the quality value. Also disclosed is a method for determining the quality value of an RNA sample wherein the relationship of the intensity of the fluorescent signal corresponding to small RNA is compared to the intensity of the fluorescent signal corresponding to large and/or structured RNA in a sample. The relationship of the intensity of the two fluorescent signals is used to determine the amount of intact RNA present in the sample.