公开(公告)号：EP2136389A4

公开(公告)日：2012-11-14

申请号：EP08720678

申请日：2008-03-28

Applicant: SHIMADZU CORP

Inventor： IWAMOTO SHINICHI ,  KODERA KEI ,  SEKIYA SADANORI

IPC: H01J49/42 ,  G01N27/62 ,  H01J49/16

CPC classification number: H01J49/424 ,  H01J49/164 ,  H01J49/4295

公开(公告)号：JP2006292602A

公开(公告)日：2006-10-26

申请号：JP2005115344

申请日：2005-04-13

Applicant: Shimadzu Corp ,  株式会社島津製作所

Inventor： FUKUYAMA HIROKO ,  IWAMOTO SHINICHI ,  TANAKA KOICHI

IPC: G01N27/62 ,  G01N33/483 ,  G01N33/68

Abstract: PROBLEM TO BE SOLVED: To provide a method capable of performing quickly and easily primary structural analysis, even if a protein or a peptide used as a mass spectrometry measuring sample has a disulfide bond. SOLUTION: In this structural analysis procedure of the protein or the peptide, MS analysis of the protein or the peptide is performed by using a reducible material to the disulfide bond as a matrix by a MALDI (matrix-assisted laser desorption ionization) mass spectrometer capable of analysis of the square or higher of MS, and MS n analysis is performed by using ions which are related to a molecular weight and acquired by the MS analysis as precursor ions, to thereby acquire amino acid sequence information of the protein or the peptide. COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 待解决的问题：即使用作质谱测量样品的蛋白质或肽具有二硫键，也可​​以提供能够进行快速且容易的初步结构分析的方法。 解决方案：在蛋白质或肽的结构分析程序中，通过使用MALDI（基质辅助激光解吸电离）作为基质的二硫键的可还原物质进行蛋白质或肽的MS分析， 能够分析MS的平方或更高的质谱仪，并且通过使用与分子量相关并且通过MS分析获得的离子作为前体离子进行MS分析，从而获得氨基 蛋白质或肽的酸序列信息。 版权所有（C）2007，JPO＆INPIT

公开(公告)号：JP2005243426A

公开(公告)日：2005-09-08

申请号：JP2004051586

申请日：2004-02-26

Applicant: Shimadzu Corp ,  株式会社島津製作所

Inventor： IWAMOTO SHINICHI

IPC: G01N27/62 ,  G01N27/64 ,  H01J49/00 ,  H01J49/06 ,  H01J49/16 ,  H01J49/40 ,  H01J49/42

CPC classification number: H01J49/424 ,  H01J49/005 ,  H01J49/40

Abstract: PROBLEM TO BE SOLVED: To provide a mass spectrometer capable of suitably selecting gas to be introduced into a collision part according to the purpose of using gas, a mass number of a sample, etc.
SOLUTION: This mass spectrometer having an ionization part ionizing the sample, a mass analysis part mass separating/detecting a sample ion and a control part controlling the movement of the whole device is provided with the collision part provided in an ion path until the sample ion generated in the ionization part is introduced into the mass analysis part and a gas introduction means introducing one or more kinds of gas selected from two or more kinds of gas into the collision part. Using such a device to analyze, high-precision mass analysis can be conducted.
COPYRIGHT: (C)2005,JPO&NCIPI

Abstract translation: 要解决的问题：提供能够根据使用气体的目的，质量数样品等适当地选择要引入碰撞部分的气体的质谱仪。解决方案：该质谱仪 具有使样品电离的离子化部分，分离/检测样品离子的质量分析部件质量和控制整个装置的运动的控制部件设置有设置在离子路径中的碰撞部分，直到在电离部分中产生的样品离子 引入质量分析部分和将选自两种或更多种气体的一种或多种气体引入到碰撞部分中的气体引入装置。 使用这种装置进行分析，可以进行高精度的质量分析。 版权所有（C）2005，JPO＆NCIPI

公开(公告)号：JP2005221328A

公开(公告)日：2005-08-18

申请号：JP2004028324

申请日：2004-02-04

Applicant: Shimadzu Corp ,  株式会社島津製作所

Inventor： IWAMOTO SHINICHI ,  SEKIYA YOSHIKI ,  TANAKA KOICHI ,  KATO KOICHI ,  TAKAHASHI REIKO ,  YAMAGUCHI YOSHIKI

IPC: G01N27/62 ,  C12Q1/48 ,  G01N27/64

Abstract: PROBLEM TO BE SOLVED: To provide a sugar chain structure analysis method for identifying a sugar chain having a branching structure, etc. as a structural isomer.
SOLUTION: This sugar chain structure analysis method is used for isotope-labelling sugar of part of a sugar chain specimen to be analyzed, capable of having a structural isomer, and performing mass spectrometry on an obtained isotope-labelled sugar chain. Preferably, the chain specimen to be analyzed has branching sugar chains and the sugar chains have the same sugar chain arrangement as each other. Further, it is preferable that end sugar residual groups of the sugar chains are isotope-labelled by using
13 C.
COPYRIGHT: (C)2005,JPO&NCIPI

Abstract translation: 待解决的问题：提供用于鉴定具有分支结构等的糖链作为结构异构体的糖链结构分析方法。 解决方案：该糖链结构分析方法用于待分析的糖链样品部分的同位素标记糖，能够具有结构异构体，并对获得的同位素标记的糖链进行质谱分析。 优选地，待分析的链样本具有分支糖链，并且糖链具有彼此相同的糖链排列。 此外，优选的是，通过使用 13 C，糖链的末端糖残基被同位素标记。 版权所有（C）2005，JPO＆NCIPI

公开(公告)号：JP2011175897A

公开(公告)日：2011-09-08

申请号：JP2010039872

申请日：2010-02-25

Applicant: Shimadzu Corp ,  株式会社島津製作所

Inventor： TANAKA KOICHI ,  IWAMOTO SHINICHI ,  KODERA KEI

IPC: H01J49/40 ,  G01N27/62 ,  H01J49/10 ,  H01J49/42

Abstract: PROBLEM TO BE SOLVED: To switch over and perform an analysis capable of obtaining a mass spectrum of a wide range of mass-to-charge ratio in a short measuring time and an analysis capable of obtaining a MS n spectrum useful for analysis of a complicated molecule structure by a single mass spectrometer. SOLUTION: An ion trap part 20 including an ion trap and a second detection part 31 are arranged between an ion introduction part 10 including a MALDI ion source, an extraction electrode 13 to accelerate ions, and an ion optical system 14 to converge ions etc. and a flight tube 34 which has a flight space in which ions are linearly flown. At the time of a normal analysis, an ion flow is stopped by the ion optical system 14 so that it may pass an ion introduction hole 24 and an ion lead-out hole 25, and the flight time is measured using the flight tube 34 interior space, as well as ion trap interior space and the space in the second detection part 31 as a free flight region. At the time of MS n analysis, the ions are trapped in the ion trap once, and the ions discharged after mass separation by the ion trap are detected by the second detection part 31. COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 要解决的问题：为了切换并执行能够在短测量时间内获得宽范围的质荷比的质谱的分析和能够获得MS n < / SP>光谱可用于通过单个质谱仪分析复杂的分子结构。 解决方案：在包括MALDI离子源的离子引入部分10，用于加速离子的提取电极13和离子光学系统14之间布置有离子阱和第二检测部分31的离子阱部分20，以收敛 离子等以及具有飞行空间的飞行管34，其中离子线性地飞行。 在正常分析时，离子流动被离子光学系统14停止，使得其可以通过离子引入孔24和离子引出孔25，并且使用飞行管34内部测量飞行时间 空间以及离子阱内部空间以及作为自由飞行区域的第二检测部31中的空间。 在MS n 分析时，将离子捕获在离子阱中一次，并且通过离子阱在质量分离后排出的离子被第二检测部31检测。版权所有： （C）2011，JPO＆INPIT

公开(公告)号：JP2007263641A

公开(公告)日：2007-10-11

申请号：JP2006087008

申请日：2006-03-28

Applicant: Shimadzu Corp ,  株式会社島津製作所

Inventor： KAJIWARA SHIGEKI ,  IWAMOTO SHINICHI ,  SEKIYA YOSHIKI

IPC: G01N27/62 ,  C07K2/00 ,  C12M1/34 ,  C12Q1/00

Abstract: PROBLEM TO BE SOLVED: To identify the amino acid sequence of each peptide with high accuracy, even if isotope peak groups derived from the plurality of peptides overlap each other, and even when the peak intensities constituting the isotope peak groups are small on MS/MS mass spectra. SOLUTION: When classifying the isotope peak groups of a peptide mixture obtained in MS/MS analysis, an element composition of ions obtained in the MS/MS analysis is estimated, to calculate the intensity ratios of each peak (S13, S14), by assuming that one, two or so on of isotopes are contained in a precursor, after inferring the element composition of precursor ions (S11). The isotope peak group is classified into a class of the isotope number contained in the precursor ions (S20), when the intensity ratios by theoretical calculation has high correlation with the peak intensity ratios, contained in the isotope peak group on the MS/MS mass spectrum actually observed ("YES" in S19), and a peak list is prepared. COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 待解决的问题：即使来自多个肽的同位素峰基团彼此重叠，即使当构成同位素峰基团的峰强度小时，也可以高精度地鉴定各肽的氨基酸序列 MS / MS质谱。 解决方案：在MS / MS分析中获得的肽混合物的同位素峰值分类时，估计在MS / MS分析中获得的离子的元素组成，以计算每个峰的强度比（S13，S14） 通过假定前体中含有一个，两个等位基因的同位素，推断出前体离子的元素组成（S11）。 同位素峰组分为前体离子中所含的同位素数（S20），当理论计算的强度比与MS / MS质谱中同位素峰组中包含的峰强度比高度相关时 实际观察到的光谱（S19中的“是”），并准备峰列表。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2006292603A

公开(公告)日：2006-10-26

申请号：JP2005115345

申请日：2005-04-13

Applicant: Shimadzu Corp ,  株式会社島津製作所

Inventor： FUKUYAMA HIROKO ,  IWAMOTO SHINICHI ,  TANAKA KOICHI

IPC: G01N27/62 ,  G01N27/64

Abstract: PROBLEM TO BE SOLVED: To provide a method capable of determining quickly and easily the number and the position of disulfide bonds in a protein or a peptide.
SOLUTION: This disulfide mapping method of the protein or the peptide is performed as follows: MS of the protein or the peptide is performed by using a reducible material to the disulfide bond by an LI mass spectrometer capable of MS of the square or higher; molecular weight related ions A acquired from the reduced disulfide bond and molecular weight related ions B acquired from the unreduced disulfide bond are acquired; a mass difference between the ions A and the ions B is calculated; the number of the disulfide bonds is estimated from the calculated mass difference; MS
n is performed by selecting the ions A and the ions B as precursor ions respectively; product ions a are acquired from the ions A, and product ions b are acquired from the ions B; amino acid sequence information is acquired from product ions a; and a mass number difference between product ions a and the product ions b is determined, to thereby estimate the position of each disulfide bond.
COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 待解决的问题：提供能够快速且容易地确定蛋白质或肽中二硫键的数目和位置的方法。 解决方案：蛋白质或肽的二硫化物测定方法如下进行：蛋白质或肽的MS通过使用能够具有正方形MS的LI质谱仪的二硫键的可还原材料进行，或 更高; 获得从还原二硫键获得的分子量相关离子A和从未还原的二硫键获得的分子量相关离子B; 计算离子A和离子B之间的质量差; 从计算的质量差估计二硫键数; 通过分别选择离子A和离子B作为前体离子来进行MS n 从离子A获得产物离子a，从离子B获得产物离子b; 从产物离子a获得氨基酸序列信息; 并且确定产物离子a和产物离子b之间的质量数差，从而估计每个二硫键的位置。 版权所有（C）2007，JPO＆INPIT

公开(公告)号：JP2006196190A

公开(公告)日：2006-07-27

申请号：JP2005003279

申请日：2005-01-11

Applicant: Shimadzu Corp ,  株式会社島津製作所

Inventor： IWAMOTO SHINICHI

IPC: H01J49/26 ,  G01N27/62 ,  G01N27/64 ,  H01J49/16 ,  H01J49/40

Abstract: PROBLEM TO BE SOLVED: To provide a method and apparatus configuration which cover a wider range of mass dimensions by a single measurement in the MALDI ion trap type mass spectrometer.
SOLUTION: A testpiece is irradiated with pulsed laser more than once while changing little by little a time duration between irradiation time t0 of pulsed laser in the MALDI section and period of time t1 when RF voltage is applied to a ring electrode attached in the ion trap section. Subsequently, summing or summing averaging is taken about the resultant mass spectrum obtained in this way. The shorter a time duration between t0 and t1 is, the more often the ion with smaller mass/charge ratio tends to be trapped into the ion trap space 122. A method of making the measurements a lot of times by shifting a time duration in this way thereby allows mass spectrum covering a wider range of mass dimensions to be obtained.
COPYRIGHT: (C)2006,JPO&NCIPI

Abstract translation: 要解决的问题：提供通过在MALDI离子阱型质谱仪中的单次测量来覆盖更宽范围的质量尺寸的方法和装置配置。

解决方案：在脉冲激光在MALDI部分的照射时间t0和RF电压施加到连接的环形电极之间的时间段t1内，在脉冲激光器上多次照射试样，同时改变脉冲激光的照射时间t0之间的持续时间 离子阱部分。 随后，对以这种方式获得的合成质谱进行求和或求和。 在t0和t1之间的时间间隔越短，质量/电荷比越小的离子越有可能倾向于被捕获到离子阱空间122中。通过在这种情况下移动持续时间来进行测量的方法很多 从而允许获得覆盖更宽范围的质量尺寸的质谱。 版权所有（C）2006，JPO＆NCIPI

公开(公告)号：JP2001070287A

公开(公告)日：2001-03-21

申请号：JP24857599

申请日：1999-09-02

Applicant: SHIMADZU CORP

Inventor： IWAMOTO SHINICHI ,  IDE SHOJI

IPC: A61B5/145 ,  A61B5/1455 ,  G01N21/17

Abstract: PROBLEM TO BE SOLVED: To prevent the generation of air layer in a contact surface between a skin surface of an organism and a probe. SOLUTION: In this somatometer for optically measuring an organism while transmitting and receiving the light to/from the organism with a probe 7, an optical matching agent is arranged in an interface between a measuring window 6 provided in an upper part of a probe 7, which is integrally provided with a light radiating part 4 for radiating the visible light or the near infrared ray to a body to be examined and a light receiver part 6 for receiving the light so as to optically measure the organism, and the skin surface of a body to be examined so as to prevent the generation of an air layer in the interface between the measuring window 6 and the skin surface. With this structure, measurement error due to the reflection of the light in an interface between the skin and the air and in an interface between the measuring window and the air can be eliminated. Since the interface between the measuring window 6 and the skin can be easily stabilized by existence of the optical matching agent, measuring time can be shortened, and multiple bodies can be measured in a short time.

公开(公告)号：JPH10295677A

公开(公告)日：1998-11-10

申请号：JP11452697

申请日：1997-05-02

Applicant: SHIMADZU CORP

Inventor： TSUNASAWA YOSHIO ,  TAKEUCHI SADAO ,  ITO YASUNOBU ,  OIKAWA YUKIO ,  IWAMOTO SHINICHI ,  TSUNEISHI SHOICHI

IPC: G01N21/49 ,  A61B5/145 ,  A61B5/1455 ,  A61B5/14

Abstract: PROBLEM TO BE SOLVED: To perform measurement of a number of specimens or a number of parts on one specimen quickly using a single unit of photo measuring device and prevent incidence of an external turbulent light into the light receiving part of a probe even in the multiple measuring. SOLUTION: A photo-measuring device is equipped with a probe 2 fitted in a single piece with a light projecting part to cast light onto a specimen and a light receiving part to receive the light diffused within the specimen, a supporting part 5 which puts the light projecting part and receiving part in contact with the specimen and supports them by means of grasping the probe, and a light shutoff part 3 which hinders an external turbulent light from being incident to the prove, at least to its light receiving part, whereby measuring is made upon pressing the probe to the specimen by grasping it, and after removal, another measuring point is measured, and in this manner, a number of specimens or a number of parts on one specimen are made measurable in a short time when incidence of external turbulent light to the light receiving part of the probe is well prevented. Thus inclusion of a measuring error originating from turbulent light is precluded, and also a measuring error associated with a movement of the measuring point precluded, and it is made practicable to measure a number of specimens or a number of parts on one specimen in a short period of time.