公开(公告)号：WO2009006369A1

公开(公告)日：2009-01-08

申请号：PCT/US2008/068714

申请日：2008-06-30

Applicant: WEYERHAEUSER COMPANY

Inventor： TIMMIS, Roger ,  GROB, James A ,  GUPTA, Pramod, K. ,  RAYFIELD, Susan D

IPC: A01H4/00

CPC classification number: A01H1/04 ,  A01C1/02 ,  A01H3/00 ,  A01H3/04 ,  A01H4/001 ,  A01H4/008

Abstract: In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) singulating a plurality of individual immature conifer somatic embryos from a first culture of embryos after a first incubation period in a first development media; and (b) contacting the plurality of singulated immature conifer somatic embryos with a second development media for a second incubation period.

Abstract translation: 在一个方面，提供了一种用于增加在体外产生的针叶树体细胞胚的发芽势的方法。 该方法包括：（a）在第一显影培养基中的第一次孵育期后，从第一胚胎培养物中分离多个单个未成熟针叶体细胞胚; 和（b）使多个单切的未成熟针叶树体细胞胚与第二培养基接触第二潜伏期。

公开(公告)号：WO1991004655A1

公开(公告)日：1991-04-18

申请号：PCT/US1990005555

申请日：1990-09-28

Applicant: WEYERHAEUSER COMPANY

Inventor： WEYERHAEUSER COMPANY ,  TIMMIS, Roger ,  KREITINGER, Mary, E. ,  YANCEY, Michael, J.

IPC: A01H04/00

CPC classification number: C12N5/04 ,  A01H4/001

Abstract: Methods and apparatuses are disclosed for growing embryonic or other undeveloped plant material into autotrophic plants. Somatic or zygotic embryos are individually embedded in sterile plugs comprised of a soil-like particulate medium. A volume of aqueous nutritive medium is added to each plug. The plugs are then isolated from the ambient environment while permitting exposure to light and atmospheric gases sufficient to effect development of photosynthesis in the developing embryos in the plugs. The resulting autotrophic plants are then removed from isolation. Subsequent watering of the growth medium removes the carbon and energy source, thereby inhibiting growth of contaminating microorganisms. The plants are then transplanted. Embodiments include compartmentalized trays and covering films by which embryo-containing plugs are kept isolated from each other and from the environment during development of the embryos.

Abstract translation: 公开了将胚胎或其他未开发的植物材料生长成自养植物的方法和装置。 将体细胞或合子胚胎分别包埋在由土壤样颗粒介质组成的无菌塞子中。 向每个塞子中加入一定体积的水性营养培养基。 然后将塞子与环境环境隔离，同时允许暴露于足以在插塞中发育的胚胎中发挥光合作用的光和大气气体。 然后从分离中除去得到的自养植物。 随后的生长培养基的浇水除去碳和能源，从而抑制污染微生物的生长。 然后移植植物。 实施方案包括分隔的托盘和覆盖膜，通过该膜，含胚的塞子在胚胎发育期间彼此隔离并且与环境保持隔离。

公开(公告)号：EP2166832B1

公开(公告)日：2012-08-15

申请号：EP08781150.1

申请日：2008-06-30

Applicant: WEYERHAEUSER COMPANY

Inventor： TIMMIS, Roger ,  GROB, James A ,  GUPTA, Pramod, K. ,  RAYFIELD, Susan D

IPC: A01H4/00

CPC classification number: A01H1/04 ,  A01C1/02 ,  A01H3/00 ,  A01H3/04 ,  A01H4/001 ,  A01H4/008

Abstract: In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) singulating a plurality of individual immature conifer somatic embryos from a first culture of embryos after a first incubation period in a first development media; and (b) contacting the plurality of singulated immature conifer somatic embryos with a second development media for a second incubation period.

公开(公告)号：EP2166832A1

公开(公告)日：2010-03-31

申请号：EP08781150.1

申请日：2008-06-30

Applicant: WEYERHAEUSER COMPANY

Inventor： TIMMIS, Roger ,  GROB, James A ,  GUPTA, Pramod, K. ,  RAYFIELD, Susan D

IPC: A01H4/00

CPC classification number: A01H1/04 ,  A01C1/02 ,  A01H3/00 ,  A01H3/04 ,  A01H4/001 ,  A01H4/008

Abstract: In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) singulating a plurality of individual immature conifer somatic embryos from a first culture of embryos after a first incubation period in a first development media; and (b) contacting the plurality of singulated immature conifer somatic embryos with a second development media for a second incubation period.