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1.发明申请METHODS FOR INCREASING GERMINATION VIGOR BY EARLY SINGULATION OF CONIFER SOMATIC EMBRYOS 审中-公开通过早期共振成像的方法增加起始位置
公开(公告)号：WO2009006369A1
公开(公告)日：2009-01-08
申请号：PCT/US2008/068714
申请日：2008-06-30
Applicant: WEYERHAEUSER COMPANY
Inventor： TIMMIS, Roger , GROB, James A , GUPTA, Pramod, K. , RAYFIELD, Susan D
IPC: A01H4/00
Abstract: In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) singulating a plurality of individual immature conifer somatic embryos from a first culture of embryos after a first incubation period in a first development media; and (b) contacting the plurality of singulated immature conifer somatic embryos with a second development media for a second incubation period.
Abstract translation: 在一个方面，提供了一种用于增加在体外产生的针叶树体细胞胚的发芽势的方法。 该方法包括：（a）在第一显影培养基中的第一次孵育期后，从第一胚胎培养物中分离多个单个未成熟针叶体细胞胚; 和（b）使多个单切的未成熟针叶树体细胞胚与第二培养基接触第二潜伏期。
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2.发明授权METHODS FOR INCREASING GERMINATION VIGOR BY EARLY SINGULATION OF CONIFER SOMATIC EMBRYOS 有权方法来增加提前退休KEIMUNGSKRAFT针叶树体细胞胚
公开(公告)号：EP2166832B1
公开(公告)日：2012-08-15
申请号：EP08781150.1
申请日：2008-06-30
Applicant: WEYERHAEUSER COMPANY
Inventor： TIMMIS, Roger , GROB, James A , GUPTA, Pramod, K. , RAYFIELD, Susan D
IPC: A01H4/00
Abstract: In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) singulating a plurality of individual immature conifer somatic embryos from a first culture of embryos after a first incubation period in a first development media; and (b) contacting the plurality of singulated immature conifer somatic embryos with a second development media for a second incubation period.
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3.发明公开METHODS FOR INCREASING GERMINATION VIGOR BY EARLY SINGULATION OF CONIFER SOMATIC EMBRYOS 有权方法来增加提前退休KEIMUNGSKRAFT针叶树体细胞胚
公开(公告)号：EP2166832A1
公开(公告)日：2010-03-31
申请号：EP08781150.1
申请日：2008-06-30
Applicant: WEYERHAEUSER COMPANY
Inventor： TIMMIS, Roger , GROB, James A , GUPTA, Pramod, K. , RAYFIELD, Susan D
IPC: A01H4/00
Abstract: In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) singulating a plurality of individual immature conifer somatic embryos from a first culture of embryos after a first incubation period in a first development media; and (b) contacting the plurality of singulated immature conifer somatic embryos with a second development media for a second incubation period.
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