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公开(公告)号：CN109486779A
公开(公告)日：2019-03-19
申请号：CN201811291824.3
申请日：2018-10-31
Applicant: 浙江大学
CPC classification number: C12N9/1007 , C12N15/70 , C12Y201/01113
Abstract: 本发明公开了一种DNA甲基转移酶及其可溶性异源表达和分离纯化方法。本发明首次从耐辐射奇球菌中分离纯化出具有DNA甲基转移酶活性并且在缓冲液中可溶的N4-Cytosine DNA甲基转移酶M.DraR1；同时还提供了该甲基转移酶的核苷酸序列和其克隆表达及分离纯化方法。本发明所提供的克隆表达及分离纯化方法可为其它DNA甲基转移酶或者限制性内切酶的开发应用提供方法学参考，对开发新型分子生物学的工具酶具有重要指导意义。
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2.发明申请METHOD FOR CLONING AND EXPRESSION OF STUI RESTRICTION ENDONUCLEASE AND STUI METHYLASE IN E. COLI 有权在大肠杆菌中克隆和表达STUI限制性内切酶和酪氨酸酶的方法公开(公告)号：US20090029418A1
公开(公告)日：2009-01-29
申请号：US12279359
申请日：2007-02-27
Applicant: Zhenyu Zhu , Shuang-Yong Xu
Inventor： Zhenyu Zhu , Shuang-Yong Xu
CPC classification number: C12N9/22 , C12N9/1007 , C12Y201/01113
Abstract: The present invention relates to compositions including: (1) isolated DNA encoding the Stul restriction endonuclease and isolated DNA encoding cognate and non-cognate methylase; (2) vectors and cells containing the isolated DNA; and (3) methods for producing the Stul restriction endonuclease.
Abstract translation: 本发明涉及组合物，其包括：（1）编码Stul限制性内切核酸酶的分离的DNA和编码同源和非同源甲基化酶的分离的DNA; （2）含有分离的DNA的载体和细胞; 和（3）生产Stul限制性内切核酸酶的方法。
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3.发明授权Method for cloning and expression of StuI restriction endonuclease and StuI methylase in E. coli 有权在大肠杆菌中克隆和表达StuI限制性内切核酸酶和StuI甲基化酶的方法公开(公告)号：US08058029B2
公开(公告)日：2011-11-15
申请号：US12279359
申请日：2007-02-27
Applicant: Zhenyu Zhu , Shuang-Yong Xu
Inventor： Zhenyu Zhu , Shuang-Yong Xu
CPC classification number: C12N9/22 , C12N9/1007 , C12Y201/01113
Abstract: The present invention relates to compositions including: (1) isolated DNA encoding the StuI restriction endonuclease and isolated DNA encoding cognate and non-cognate methylase; (2) vectors and cells containing the isolated DNA; and (3) methods for producing the StuI restriction endonuclease.
Abstract translation: 本发明涉及组合物，其包括：（1）编码StuI限制性内切核酸酶的分离的DNA和编码同源和非同源甲基化酶的分离的DNA; （2）含有分离的DNA的载体和细胞; 和（3）生产StuI限制性内切核酸酶的方法。
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4.发明公开METHOD FOR CLONING AND EXPRESSION OF STUI RESTRICTION ENDONUCLEASE AND STUI METHYLASE IN E. COLI 审中-公开用于克隆方法和StuI限制性内切酶的表达和甲基化酶的StuI在大肠杆菌
公开(公告)号：EP1989298A4
公开(公告)日：2009-04-29
申请号：EP07751832
申请日：2007-02-27
Applicant: NEW ENGLAND BIOLABS INC
Inventor： XU SHUANG-YONG , ZHU ZHENYU
IPC: C12N9/10
CPC classification number: C12N9/22 , C12N9/1007 , C12Y201/01113
Abstract: The present invention relates to compositions including: (1) isolated DNA encoding the Stul restriction endonuclease and isolated DNA encoding cognate and non-cognate methylase; (2) vectors and cells containing the isolated DNA; and (3) methods for producing the Stul restriction endonuclease.
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Patent Agency Ranking
公开(公告)号：EP4437092A1
公开(公告)日：2024-10-02
申请号：EP22834472.7
申请日：2022-11-25
Applicant: Epigenic Therapeutics Inc.
Inventor： ZHOU, Changyang , SUN, Yidi , PENG, Wenbo , MAO, Shaoshuai
CPC classification number: C12N9/22 , C12Y304/21 , C12N9/6424 , C07K2319/8120130101 , C12N15/1137 , C12Y201/01037 , C12Y201/01072 , C12Y201/01113 , C07K2319/0020130101 , A61P3/06 , A61P9/10 , A61P3/10 , C07K2319/7120130101 , C12N2310/2020170501 , A61K38/45
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6.发明公开METHOD FOR CLONING AND EXPRESSION OF STUI RESTRICTION ENDONUCLEASE AND STUI METHYLASE IN E. COLI 审中-公开用于克隆方法和StuI限制性内切酶的表达和甲基化酶的StuI在大肠杆菌
公开(公告)号：EP1989298A2
公开(公告)日：2008-11-12
申请号：EP07751832.2
申请日：2007-02-27
Applicant: NEW ENGLAND BIOLABS, INC.
Inventor： XU, Shuang-yong , ZHU, Zhenyu
IPC: C12N9/10
CPC classification number: C12N9/22 , C12N9/1007 , C12Y201/01113
Abstract: The present invention relates to compositions including: (1) isolated DNA encoding the Stul restriction endonuclease and isolated DNA encoding cognate and non-cognate methylase; (2) vectors and cells containing the isolated DNA; and (3) methods for producing the Stul restriction endonuclease.
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7.发明专利
公开(公告)号：JP2009528839A
公开(公告)日：2009-08-13
申请号：JP2008558299
申请日：2007-02-27
Applicant: ニユー・イングランド・バイオレイブス・インコーポレイテツド
Inventor： シユイ，シユワン−ヨン , ジユウ，ジエンユ
CPC classification number: C12N9/22 , C12N9/1007 , C12Y201/01113
Abstract: 本発明は（１）ＳｔｕＩ制限エンドヌクレアーゼをコードする単離ＤＮＡとコグネイト及び非コグネイトメチラーゼをコードする単離ＤＮＡ；（２）前記単離ＤＮＡを含むベクター及び細胞；並びに（３）ＳｔｕＩ制限エンドヌクレアーゼの生産方法を含む構成に関する。
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8.发明申请METHOD FOR CLONING AND EXPRESSION OF STUI RESTRICTION ENDONUCLEASE AND STUI METHYLASE IN E. COLI 审中-公开在大肠杆菌中克隆和表达STUI限制性内切酶和酪氨酸酶的方法
公开(公告)号：WO2007103061A3
公开(公告)日：2008-06-26
申请号：PCT/US2007005099
申请日：2007-02-27
Applicant: NEW ENGLAND BIOLABS INC , XU SHUANG-YONG , ZHU ZHENYU
Inventor： XU SHUANG-YONG , ZHU ZHENYU
IPC: C12N9/10
CPC classification number: C12N9/22 , C12N9/1007 , C12Y201/01113
Abstract: The present invention relates to compositions including: (1) isolated DNA encoding the Stul restriction endonuclease and isolated DNA encoding cognate and non-cognate methylase; (2) vectors and cells containing the isolated DNA; and (3) methods for producing the Stul restriction endonuclease.
Abstract translation: 本发明涉及组合物，其包括：（1）编码Stul限制性内切核酸酶的分离的DNA和编码同源和非同源甲基化酶的分离的DNA; （2）含有分离的DNA的载体和细胞; 和（3）生产Stul限制性内切核酸酶的方法。
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9.发明申请METHOD FOR CLONING AND EXPRESSION OF STUI RESTRICTION ENDONUCLEASE AND STUI METHYLASE IN E. COLI 审中-公开在大肠杆菌中克隆和表达STUI限制性内切酶和酪氨酸酶的方法
公开(公告)号：WO2007103061A2
公开(公告)日：2007-09-13
申请号：PCT/US2007/005099
申请日：2007-02-27
Applicant: NEW ENGLAND BIOLABS, INC. , XU, Shuang-yong , ZHU, Zhenyu
Inventor： XU, Shuang-yong , ZHU, Zhenyu
IPC: C12N9/10
CPC classification number: C12N9/22 , C12N9/1007 , C12Y201/01113
Abstract: The present invention relates to compositions including: (1) isolated DNA encoding the Stul restriction endonuclease and isolated DNA encoding cognate and non-cognate methylase; (2) vectors and cells containing the isolated DNA; and (3) methods for producing the Stul restriction endonuclease.
Abstract translation: 本发明涉及组合物，其包括：（1）编码Stul限制性内切核酸酶的分离的DNA和编码同源和非同源甲基化酶的分离的DNA; （2）含有分离的DNA的载体和细胞; 和（3）生产Stul限制性内切核酸酶的方法。
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