公开(公告)号：US20140073756A1

公开(公告)日：2014-03-13

申请号：US14081224

申请日：2013-11-15

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Nini H. KJUS ,  Geir FONNUM ,  Bruce BRANCHAUD ,  Lai-Qiang Ying ,  Steven M. MENCHEN ,  Dmitriy GREMYACHINSKIY ,  Hee Chol KANG

IPC: C07F7/18 ,  C08F236/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

Abstract translation: 描述了甲硅烷基保护的二丙烯酰胺化合物。 形成这种化合物的方法包括在第一反应条件下将甲硅烷基化试剂与羟基化二胺化合物混合以在第一溶液中形成产物，将产物与第一溶液分离，并在第二反应条件下将产物与丙烯酰氯混合 形成甲硅烷基保护的二丙烯酰胺化合物的第二溶液。

公开(公告)号：US08658733B2

公开(公告)日：2014-02-25

申请号：US13649045

申请日：2012-10-10

Applicant: Life Technologies AS ,  Arvid Tygve Berge ,  Viola Ugelstad

Inventor： Arne Jorgedal ,  Elin Aksnes ,  Geir Fonnum ,  Astrid Molteberg ,  Rolf Nordal ,  Henning Pettersen ,  Tollef Taarneby ,  Solveig Staale ,  Ellen Weng ,  Finn Hansen ,  Silje Nordbo ,  Oddvar Aune ,  Arvid Berge ,  John Bjorgum ,  Turid Ellingsen ,  John Ugelstad

IPC: C08F8/30

CPC classification number: C08J9/16 ,  C08F2/22 ,  C08F257/02 ,  C08F265/04 ,  C08F265/10 ,  C08F291/00 ,  C08J9/22 ,  C08J9/224 ,  C08J2203/14 ,  C08J2325/06 ,  C08J2347/00

Abstract: A process for the preparation of monodisperse polymer particles which are formed by contacting monomers with aqueous dispersions comprising monodisperse swellable seed polymers/oligomers, and initiating polymerization in the presence of a steric stabilizer. The resulting swollen seed particles are characterized by the particle mode diameter.

Abstract translation: 通过使单体与包含单分散可溶胀种子聚合物/低聚物的水性分散体接触而形成的单分散聚合物颗粒的制备方法，以及在空间稳定剂存在下引发聚合。 所得的溶胀种子颗粒的特征在于颗粒模式直径。

公开(公告)号：WO2022081519A1

公开(公告)日：2022-04-21

申请号：PCT/US2021/054490

申请日：2021-10-12

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES HOLDINGS PTE LIMITED

Inventor： LUHR, Morten ,  GORDON, Michael ,  SIA, Ming Tiong ,  CHONG, Kok Shyong ,  SOH, Woon Liang Terence ,  LIM, Seng Leong ,  SCHROEDER, Ida Caroline ,  ALFSNES, Katrine ,  LIM, Terry JianHui ,  BOSNES, Marie ,  NEURAUTER, Axl Alois ,  PEDERSEN, Ketil Winther ,  WILBUR, Autumn Day ,  ZIMMERMAN, Sean

IPC: G01N35/00 ,  G01N33/543 ,  C12M1/00

Abstract: A bead processing assembly for use in attaching magnetic beads to biological cells and other biological materials and/or separating magnetic beads from biological cells and other biological materials includes a base assembly having a housing, a support panel disposed on the housing and having a front face, a first pinch valve at least partially outwardly projecting from the front face of the support panel, and a first pump at least partially outwardly projecting from the front face of the support panel. A rocker assembly is supported on the base assembly and includes a mount assembly supported on the base assembly, a platform assembly pivotably secured to the mount assembly, and a rocker drive for repeatedly rocking the platform assembly relative to the mount assembly.

公开(公告)号：WO2013171123A1

公开(公告)日：2013-11-21

申请号：PCT/EP2013/059675

申请日：2013-05-08

Applicant: LIFE TECHNOLOGIES AS

Inventor： NOKLEBY, Lars ,  REED, Berit ,  HILDISCH, Tine ,  NEURAUTER, Axl

IPC: B01L9/06 ,  G01N33/543 ,  G01N35/00

CPC classification number: B03C1/14 ,  B01L9/06 ,  B03C1/0332 ,  B03C1/0335 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  G01N35/0098

Abstract: This invention relates to a sample holder for isolating magnetically labelled particles from a non-magnetic medium in a plurality of samples. The holder comprises a magnetic base for applying a magnetic force to the magnetically labelled particles and a body which is mountable on the base and demountable therefrom. The body comprises an array of sample holding portions and a magnetisable member which is magnetically urged towards the magnetic base when the body is seated on the base, whereby the body is urged to remain seated on the base. The invention also relates to use of the sample holder to separate magnetic particles from a non-magnetic medium and methods of performing such a separation.

Abstract translation: 本发明涉及用于从多个样品中的非磁性介质中分离磁性标记的颗粒的样品保持器。 保持器包括用于向磁性标记的颗粒施加磁力的磁性基座和可安装在基座上并可由其拆卸的主体。 身体包括一组样本保持部分和一个可磁化部件，当身体坐在基座上时，可磁化部件被磁力推向磁性基座，由此推动身体保持坐在基部上。 本发明还涉及样品保持器从非磁性介质中分离磁性颗粒的使用以及进行这种分离的方法。

公开(公告)号：WO2016094512A1

公开(公告)日：2016-06-16

申请号：PCT/US2015/064700

申请日：2015-12-09

Applicant: yLIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH ,  LIFE TECHNOLOGIES AS

Inventor： POEHMERER, Thomas ,  KUHN, Phillip ,  NOTKA, Frank ,  ZEIDLER, Andreas ,  HEIL, Korbinian ,  TREFZER, Axel ,  FONNUM, Geir ,  KATZEN, Federico ,  ANDERSSON, Kristian ,  LIANG, Xiquan

IPC: B01L3/00 ,  G01N35/00 ,  B03C5/02

CPC classification number: C12N15/101 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00653 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2300/0893 ,  B01L2400/046 ,  B01L2400/0487 ,  B03C5/026 ,  B03C2201/26 ,  C25B3/10 ,  C25B9/18 ,  C25B11/04 ,  C25B15/02

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

Abstract translation: 本公开一般涉及用于生产核酸分子的组合物和方法。 在一些方面，本发明允许核酸分子的微量生成，任选地随后将这些核酸分子装配到更大的分子中。 在一些方面，本发明允许有效生产核酸分子（例如，大的核酸分子，例如基因组）。

公开(公告)号：WO2018134374A3

公开(公告)日：2018-07-26

申请号：PCT/EP2018/051349

申请日：2018-01-19

Applicant: LIFE TECHNOLOGIES AS

Inventor： LUNDBERG, Per, Nils, Pontus, ,  FONNUM, Geir, ,  ANDERSSON, Kristian, H,

IPC: C08F120/54 ,  C08F2/38 ,  C08F220/34 ,  C08F265/10 ,  C08L33/26

Abstract: This invention relates to monodisperse magnetic hydrogel polymer particles comprising a magnetic material and a polymer formed from (a) a hydrophilic vinylic monomer having a log P oct/wat (log P) of less than about 0.5; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse coated hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer having a log P oct/wat (log P) of less than about 0.5; and (b) a crosslinker comprising at least two vinyl groups; and a coating. Also provided are methods of forming the monodisperse magnetic hydrogel polymer particles and monodisperse coated polymer particles.

公开(公告)号：WO2022101304A1

公开(公告)日：2022-05-19

申请号：PCT/EP2021/081305

申请日：2021-11-10

Applicant: LIFE TECHNOLOGIES AS

Inventor： JOBERT, Laure ,  BOSNES, Marie ,  LINDSTRØM, Hannah, Kim

IPC: C12N15/10

Abstract: A method of separating RNA from a sample, comprising providing: a sample comprising RNA, a binding solution comprising an oligoethylene glycol and a salt, and a solid support having a hydrophilic surface. The method further comprises contacting the sample with the binding solution and solid support, under conditions that allow binding of the RNA in the sample to the surface of the solid support, thereby providing a solid support with bound RNA in contact with residual solution; and separating the solid support with bound RNA from the residual solution. During the binding of the RNA to the surface of the solid support, oligoethylene glycol is present at a concentration of at least about 35% v/v and the salt is present at a concentration of between about 1 M and about 2 M. Methods for producing purified RNA are also provided, comprising in vitro transcription to produce an RNA molecule with a first magnetic bead and purification with a second magnetic bead. Also provided are kits and uses.

公开(公告)号：WO2019048333A1

公开(公告)日：2019-03-14

申请号：PCT/EP2018/073353

申请日：2018-08-30

Applicant: LIFE TECHNOLOGIES AS

Inventor： JOBERT, Laure

IPC: C07H21/00 ,  C12N15/10 ,  C12Q1/6834

Abstract: This invention relates to methods and compositions for coupling nucleic acid to a functionalized surface or support. In particular, the present invention provides an improved process for coupling aminated nucleic acid to a support functionalized with carboxylic acid groups, wherein the coupling reaction is conducted in the presence of an organic solvent. The invention further relates to compositions and kits for performing the coupling reaction and uses of nucleic acid-loaded supports for various applications.

公开(公告)号：WO2018134374A2

公开(公告)日：2018-07-26

申请号：PCT/EP2018/051349

申请日：2018-01-19

Applicant: LIFE TECHNOLOGIES AS

Inventor： LUNDBERG, Per, Nils, Pontus, ,  FONNUM, Geir, ,  ANDERSSON, Kristian, H,

IPC: C08F120/54 ,  C08F2/38 ,  C08F220/34 ,  C08F265/10 ,  C08L33/26

Abstract: This invention relates to monodisperse magnetic hydrogel polymer particles comprising a magnetic material and a polymer formed from (a) a hydrophilic vinylic monomer having a log P oct/wat (log P) of less than about 0.5; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse coated hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer having a log P oct/wat (log P) of less than about 0.5; and (b) a crosslinker comprising at least two vinyl groups; and a coating. Also provided are methods of forming the monodisperse magnetic hydrogel polymer particles and monodisperse coated polymer particles.

公开(公告)号：WO2017167716A1

公开(公告)日：2017-10-05

申请号：PCT/EP2017/057253

申请日：2017-03-28

Applicant: LIFE TECHNOLOGIES AS

Inventor： ELLIS, Darren ,  LINDSTROEM, Hannah

IPC: C12N15/10

Abstract: The disclosure generally relates to compositions and methods for the extraction of nucleic acids from biological samples. In particular embodiments the extraction involves the use of an organic solvent with sufficient density so that centrifugation of the sample is not needed to achieve phase separation.

Abstract translation: 本公开总体上涉及用于从生物样品中提取核酸的组合物和方法。 在具体的实施方案中，提取包括使用具有足够密度的有机溶剂，从而不需要离心样品以实现相分离。