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公开(公告)号：KR1020130040509A
公开(公告)日：2013-04-24
申请号：KR1020110105330
申请日：2011-10-14
Applicant: 한국화학연구원
CPC classification number: C12P7/62
Abstract: PURPOSE: A method for preparing polyhydroxyalkanoate from a saccharified solution of Hydrodictyaceae algal biomass is provided to cheaply saccharify and to produce biochemical products. CONSTITUTION: A method for preparing polyhydroxyalkyanoate from a saccharified solution of Hydrodictyaceae algal biomass comprises a step of saccharifying Hydrodictyaceae algal biomass and preparing a saccharified solution containing monosaccharides. The Hydrodictyaceae algae are selected from the group consisting of Hydrodictyon sp., Pediastrum sp., and Sorastrum sp. The saccharification is performed using a hydrolysis catalyst, a polysaccharide decomposition enzyme, or a mixture thereof. The polysaccharide decomposition enzyme is selected from the group consisting of cellulase, beta-glucosidase, beta-agarase, betas-gallactosidase, endo-1,4-beta-glucanase, alpha-amylase, beta-amylase, amyloglucosidase, alpha-D-glucosidase, beta-D-glucosidase, sucrase, maltase, isomaltase, and lactase. [Reference numerals] (AA) Collected algal biomass; (BB) Previously extracting effective materials using water or a solvent; (CC) Powderization, drying, and sterilization; (DD) Collected algal biomass(living body, dried body); (EE) Algal solid after extraction; (FF) Saccharifying complex enzyme; (GG) Chemical hydrolyzing; (HH) Low concentration saccharified liquid; (II) Separating saccharified liquid, filtering, concentrating(reducing fermentation suppressing materials); (JJ) High quality sugar concentrated liquid; (KK) Culturing microorganisms for producing PHAs; (LL) Gathering the microorganisms, separating the PHAs, and refining; (MM) Producing polyhydroxyalkanoate;
Abstract translation: 目的：提供从Hydrodictyaceae藻类生物质的糖化溶液中制备聚羟基链烷酸酯的方法，以便廉价糖化并生产生物化学产品。 构成：从Hydrodictyaceae藻类生物质的糖化溶液中制备聚羟基链烷酸酯的方法包括将Hydrodictyaceae藻类生物质糖化并制备含有单糖的糖化溶液的步骤。 Hydrodictyaceae藻类选自Hydrodictyon sp。，Pediastrum sp。和Sorastrum sp。 糖化使用水解催化剂，多糖分解酶或其混合物进行。 多糖分解酶选自纤维素酶，β-葡糖苷酶，β-琼脂糖酶，β-葡萄糖苷酶，内切-1,4-β-葡聚糖酶，α-淀粉酶，β-淀粉酶，淀粉葡糖苷酶，α-D-葡萄糖苷酶 ，β-D-葡糖苷酶，蔗糖酶，麦芽糖酶，异麦芽糖酶和乳糖酶。 （附图标记）（AA）收集的藻类生物质; （BB）以前用水或溶剂提取有效的物质; （CC）粉化，干燥和灭菌; （DD）收集的藻类生物质（活体，干体）; （EE）提取后的藻类固体; （FF）糖化复合酶; （GG）化学水解; （HH）低浓度糖化液; （二）分离糖化液，过滤，浓缩（减少发酵抑制物质）; （JJ）优质糖浓缩液; （KK）培养生产PHA的微生物; （LL）收集微生物，分离PHA，精制; （MM）生产聚羟基链烷酸酯;
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公开(公告)号：KR1020120103958A
公开(公告)日：2012-09-20
申请号：KR1020110021962
申请日：2011-03-11
Applicant: 한국화학연구원
CPC classification number: C12N9/0008 , C12N9/1096 , C12P13/005 , C12Y206/01019
Abstract: PURPOSE: A method for preparing gamma-amino butyric acid(GABA) from glucose using recombinant E.coli is provided to ensure conversion productivity from glucose to GABA. CONSTITUTION: A method for producing recombinant E.coli for producing GABA(gamma-amino butyric acid) comprises: a step of preparing E.coli in which a gene encoding GabD(succinate-semialdehyde dehydrogenase) and YneI(succinate-semialdehyde dehydrogenase); and a step of transforming the E.coli with a recombinant vector containing a gene encoding succinic semialdehyde dehydrogenase(SucD) and 4-aminobutyrate aminotransferase(GabT).
Abstract translation: 目的：提供使用重组大肠杆菌从葡萄糖制备γ-氨基丁酸（GABA）的方法，以确保葡萄糖转化为GABA的转化率。 构成：制备用于产生GABA（γ-氨基丁酸）的重组大肠杆菌的方法包括：制备大肠杆菌的步骤，其中编码GabD（琥珀酸半醛脱氢酶）和YneI（琥珀酸 - 半醛脱氢酶）的基因; 和用含有编码琥珀酸半醛脱氢酶（SucD）和4-氨基丁酸转氨酶（GabT）的基因的重组载体转化大肠杆菌的步骤。
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公开(公告)号：KR1020120036635A
公开(公告)日：2012-04-18
申请号：KR1020100098414
申请日：2010-10-08
Applicant: 한국화학연구원
CPC classification number: C12N9/16 , C07K2319/20 , C07K2319/50
Abstract: PURPOSE: A novel psychrophilic esterase is provided to ensure high activity at pH 8-10 and to be used in various industries. CONSTITUTION: A polypeptide with esterase activity is a polypeptide: which is encoded by a polynucleotide of sequence number 5; which is encoded by a polynucleotide hybridizable with a polynucleotide of sequence number 5 under a strict condition; or which has an amino acid sequence of sequence number 3. A method for preparing a recombinant esterase comprises: a step of preparing a recombinant expression vector containing the polynucleotide with esterase activity; a step of introducing the recombinant expression vector to a host cell to prepare a transformant; and a step of culturing the transformant to express the recombinant proteins and collecting the recombinant proteins.
Abstract translation: 目的：提供一种新颖的嗜酸性酯酶，以确保pH 8-10的高活性，并用于各种行业。 构成：具有酯酶活性的多肽是多肽，其由序列号5的多核苷酸编码; 其由在严格条件下可与序列号5的多核苷酸杂交的多核苷酸编码; 或其具有序列号3的氨基酸序列。制备重组酯酶的方法包括：制备含有具有酯酶活性的多核苷酸的重组表达载体的步骤; 将重组表达载体导入宿主细胞以制备转化体的步骤; 以及培养转化体以表达重组蛋白并收集重组蛋白的步骤。
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公开(公告)号：KR1020110013726A
公开(公告)日：2011-02-10
申请号：KR1020090071330
申请日：2009-08-03
Applicant: 한국화학연구원
IPC: C07K14/00
Abstract: PURPOSE: A thermally stable CiP(Coprinus cinereus peroxidase) mutant protein and a DNA encoding the same are provided to ensure high thermal stability and to be used in various industries. CONSTITUTION: A thermally stable CiP(Coprinus cinereus peroxidase) mutant protein has 323th amino acid of sequence number 1, in which serine is substituted with tyrosine; 328th amino acid in which glutamic acid is substituted with aspartic acid; or both thereof. A DNA encoding CiP mutant protein has 967-969th bases of sequence number 5, which are substituted with a codon encoding tyrosine and has a base sequence of sequence number 6.
Abstract translation: 目的：提供热稳定的CiP（鬼马菌过氧化物酶）突变蛋白和编码它的DNA，以确保高热稳定性并用于各种行业。 构成：热稳定的CiP（鬼马菌过氧化物酶）突变蛋白具有序列号1的323位氨基酸，其中丝氨酸被酪氨酸取代; 谷氨酸被天冬氨酸取代的第328位氨基酸; 或其两者。 编码CiP突变蛋白的DNA具有序列号5的967-969位碱基，其被编码酪氨酸的密码子取代并具有序列号6的碱基序列。
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公开(公告)号：KR100986733B1
公开(公告)日：2010-10-08
申请号：KR1020080078005
申请日：2008-08-08
Applicant: 한국화학연구원
Abstract: 본 발명은 증진된 부탄올 내성 및 부탄올 고생산 균주의 제조방법에 관한 것으로, 구체적으로 클로스트리디움 베이저링키(
Clostridium
beijerinckii ) NCIMB 8052 야생형 균주를 돌연변이 시킨 후, 세포막 합성저해 화학물질에 내성을 갖으며, 고농도로 부탄올을 생산할 수 있는 균주를 스크리닝하여 선별된, 부탄올 내성 및 부탄올 고생산 균주에 관한 것이다. 본 발명의 방법에 의해 제조된 부탄올 내성 및 부탄올 고생산 균주는 바이오 부탄올 상업화 기술에 매우 유용하게 이용될 수 있다.
부탄올, 클로스트리디움 베이저링키, 돌연변이, 대량생산, 바이오 부탄올-
Patent Agency Ranking
公开(公告)号：KR1020090095892A
公开(公告)日：2009-09-10
申请号：KR1020080021115
申请日：2008-03-06
Applicant: 한국화학연구원
IPC: C07C29/147 , C07C31/02
CPC classification number: C07C29/149 , B01J23/626 , B01J23/6567 , B01J23/8966 , B01J37/0009 , B01J37/031 , B01J37/10 , B01J37/18 , B01J2523/00 , C07C31/12 , B01J2523/27 , B01J2523/43 , B01J2523/821 , B01J2523/74 , B01J2523/828 , B01J2523/845 , B01J2523/17 , B01J2523/47 , B01J2523/41
Abstract: A method for manufacturing a monovalent alcohol is provided to improve selectivity and space yield even under the mild reaction condition by using a catalyst having excellent thermal stability, chemical stability and reaction activity. A method for manufacturing a monovalent alcohol comprises the step of direct gas phase reducing a C1~C10 monocarboxylic acid or its derivative by hydrogen on a catalyst represented by Ru(a)Sn(b)Zn(d)Ox, wherein (a), (b) and (d) are a component ratio based on an atomic number, and (a) is 1~20 and (b) is 1~40 if (d) is 100; and x is an atomic number of oxygen and is determined according to the valence and composition ratio of other components. The catalyst is plasticized at 300~800 °C under the air atmosphere.
Abstract translation: 提供一价醇的制造方法，通过使用具有优异的热稳定性，化学稳定性和反应活性的催化剂，即使在温和的反应条件下也能提高选择性和空间收率。 一价醇的制造方法包括在由Ru（a）Sn（b）Zn（d）Ox表示的催化剂上直接气相还原C1〜C10一元羧酸或其衍生物的工序，其中（a）， （b）和（d）是基于原子序数的成分比例，（a）如果（d）为100，（a）为1〜20，（b）为1〜40; x是氧的原子数，根据其他成分的化合价和成分比来确定。 催化剂在空气气氛下在300〜800℃下塑化。
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公开(公告)号：KR100597682B1
公开(公告)日：2006-07-07
申请号：KR1020040103608
申请日：2004-12-09
Applicant: 한국화학연구원
CPC classification number: C08G65/38 , B01J31/04 , B01J2531/72 , B01J2531/845 , C08G65/44
Abstract: 본 발명은 코프리너스 시네레우스 유래 퍼옥시다아제를 이용한 페놀계 고분자의 제조방법에 관한 것으로서, 더욱 상세하게는 페놀 단량체 중합시 유독성의 포르말린 사용이 필수적인 통상의 퍼옥시다아제와 가격이 매우 고가이고 과산화수소에 의하여 쉽게 불활성화되는 식물체 유래 퍼옥시다아제 대신에, 코프리너스 시네레우스 유래 퍼옥시다아제를 이용하여 페놀계 단량체를 극성 유기용매 내에서 온화한 반응조건(상온, 상압) 하에서 중합시킴으로써 수율이 증대되고, 경제적이며, 내수성, 내화학성이 뛰어난 페놀계 고분자를 제조하는 방법에 관한 것이다.
퍼옥시다아제, 페놀계 고분자, 코프리너스 시네레우스-
公开(公告)号：KR100535950B1
公开(公告)日：2005-12-09
申请号：KR1020030051034
申请日：2003-07-24
Applicant: 한국화학연구원
IPC: C08G61/00
Abstract: 본 발명은 생촉매를 이용한 페놀계 중합체의 제조방법 및 그의 용도에 관한 것으로서, 보다 상세하게는 식물에서 유래한 페놀계 단량체를 생촉매인 산화환원 효소의 작용으로 유기용매 내에서 중합시킴으로써 효과적으로 페놀계 중합체를 제조하며, 이를 경화시킨 후 방오도료에 적용할 경우 상기 방오도료를 코팅하여 형성된 페놀계 고분자 코팅 도막의 표면 에너지가 작아서 물리적인 해양생물의 부착을 저해하고, 또한, 방오작용기의 소모가 발생되지 않아 지속적으로 내구성을 발현하는 화학적 방오작용이 우수한 특성을 나타내는 생촉매를 이용한 페놀계 중합체의 제조방법 및 그의 용도에 관한 것이다.
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公开(公告)号：KR100493713B1
公开(公告)日：2005-06-03
申请号：KR1020010066230
申请日：2001-10-26
Applicant: 한국화학연구원
Abstract: 본 발명은 수중오염물질을 제거 처리하기 위한 고정화 이산화티탄 광촉매의 제조방법에 관한 것으로서, 더욱 상세하게는 나노크기의 이산화티탄 분말을 수중에서 음이온성 분산제로 분산안정화시키고, 이를 중공형 또는 다공성의 무기담체에 실리콘계 또는 알루미나계 바인더로 부착시켜 만든 고정화된 이산화티탄 광촉매의 제조방법에 관한 것이다. 상기의 방법으로 제조된 광촉매를 수처리장치에 적용함으로써 광촉매를 회수하기 위한 별도의 분리장치가 필요없고, 동시에 광촉매의 분산으로 인한 균일한 광조사 효과를 얻을 수 있어 경제적이면서도 대용량으로 확장이 용이하다.
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公开(公告)号：KR1020040094496A
公开(公告)日：2004-11-10
申请号：KR1020030028284
申请日：2003-05-02
Applicant: 한국화학연구원
IPC: C02F1/72
Abstract: PURPOSE: To provide a superior photocatalyst which can be reused by adding boracic acid and sodium ethoxide, thereby improving immobilization force of titanium dioxide and media, a water treatment apparatus for further improving efficiency of the photocatalyst by easily discharging purified treated water, and a water treatment method using the photocatalyst. CONSTITUTION: In a water treatment apparatus comprising a hollow reactor (10) having a certain volume, a raw water inflow pump (22) for flowing raw water in the reactor, and a transfer pump (26) for transferring treated water from the reactor, the water treatment apparatus using immobilized photocatalyst comprises a cylindrical rotary screen (12) vertically arranged in a central part inside the reactor in such a way that the rotary screen is rotated; vanes (18) attached to upper and lower end parts of the rotary screen; a variable motor (20) connected to a hollow rotary shaft by pulley and belt to provide the rotary screen with rotary force, wherein the hollow rotary shaft is integrally attached to an upper part of the rotary screen; a plurality of ultraviolet lamps (14) vertically arranged between the inner wall of the reactor and the rotary screen; and a pair of air lift pumps (16) communicatingly connected to the reactor, wherein the treated water is transferred by the transfer pump as the treated water is passing through the hollow rotary shaft in an upper part of the rotary screen from the rotary screen.
Abstract translation: 目的：提供一种可以通过添加硼酸和乙醇钠重新使用的优异的光催化剂，从而提高二氧化钛和介质的固定力，一种水处理设备，用于通过容易地排出经过净化的水，进一步提高光催化剂的效率，以及水 使用光催化剂的处理方法。 构成：在具有一定体积的中空反应器（10）的水处理装置中，将用于使反应器内的原水流动的原水流入泵（22）和从反应器输送处理水的输送泵（26） 使用固定化光催化剂的水处理装置包括垂直设置在反应器内的中心部分的圆筒形旋转筛（12），使得旋转筛旋转; 安装在旋转筛的上端部和下端部的叶片（18） 一个可变马达（20），其通过皮带轮和皮带带连接到空心旋转轴上，以向旋转屏幕提供旋转力，其中空心旋转轴一体地附接到旋转筛的上部; 垂直设置在反应器的内壁和旋转筛之间的多个紫外线灯（14）; 以及一对与反应器连通的空气提升泵（16），其中处理过的水在旋转筛网的上部通过旋转筛网的上部的中空旋转轴时，经处理后的水被转移。
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