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    ALKALI-ALPHA-AMYLASE, MICROORGANISM PRODUCING THE SAME AND PRODUCTION OF THE SAME ALKALI ALPHA-AMYLASE
    91.
    发明专利
    ALKALI-ALPHA-AMYLASE, MICROORGANISM PRODUCING THE SAME AND PRODUCTION OF THE SAME ALKALI ALPHA-AMYLASE 失效

    公开(公告)号：JPH09206073A

    公开(公告)日：1997-08-12

    申请号：JP2099996

    申请日：1996-02-07

    Applicant: KAO CORP

    Inventor： IGARASHI KAZUAKI ,  HAGIWARA HIROSHI ,  SAWADA KAZUHISA ,  ARA KATSUTOSHI ,  KAWAI SHUJI ,  ITO SUSUMU

    IPC: C12N9/28 ,  C12N1/20 ,  C12R1/07

    Abstract: PROBLEM TO BE SOLVED: To obtain a new alkali α-amylase having optimal condition or resistance to high alkali, having resistance to detergent component and useful as an additive component for detergents for dishes and detergents for clothes. SOLUTION: This alkali α-amylase is obtained by culturing Bacillus sp. KSM-SAA1112 (FERM P-15200), etc., which is an alkalophilic bacterium and has the following enzymatic properties: (1) capable of decomposing α-1,4 glucoside bond of starch, amylose, amylopectin and their partial decomposition product and not reacting with pullulan; (2) optimal pH 10-11 and acting in a range of pH 6.5 to 12.5 and extremely stable in a range of pH11.5; (3) 60 deg.C optimal temperature and capable of acting in a range of 20-70 deg.C; (4) 60,000±3,000 molecular weight; (5) about 4.1 isoelectric point; and (6) being hardly inhibited by surfactants.

    LIQUEFIED-TYPE ALKALI ALPHA-AMYLASE GENE
    92.
    发明专利
    LIQUEFIED-TYPE ALKALI ALPHA-AMYLASE GENE 失效

    公开(公告)号：JPH08336392A

    公开(公告)日：1996-12-24

    申请号：JP14725795

    申请日：1995-06-14

    Applicant: KAO CORP

    Inventor： HATADA YUUJI ,  OZAKI KATSUYA ,  ARA KATSUTOSHI ,  KAWAI SHUJI ,  ITO SUSUMU

    IPC: C12N15/09 ,  C12N1/21 ,  C12N9/28 ,  C12N15/56 ,  C12R1/07 ,  C12R1/19

    Abstract: PURPOSE: To obtain a gene for industrially mass-produce liquefied-type alkali α-amylase useful as an additive ingredient for detergent compositions for tableware and clothing. CONSTITUTION: This liquefied-type alkali α-amylase gene having a nucleotide sequence of the formula is obtained by breaking a chromosome DNA obtained from Bacillus sp. KSM-AP 1378 (FERM BP-3048) with a restriction enzyme such as Hind III followed by conducting PCR process. Plasmid pAML 100 is a recombinant plasmid composed of a 1.8kb fragment containing this gene and pUC18 4.4kb fragment, and a recombinant microbe containing the recombinant DNA molecule is pref. Escherichia coli HB 101 (pAML100) strain.

    SACCHARIFICATION OF POLYSACCHARIDE
    94.
    发明专利
    SACCHARIFICATION OF POLYSACCHARIDE 失效

    公开(公告)号：JPH0494694A

    公开(公告)日：1992-03-26

    申请号：JP21195590

    申请日：1990-08-10

    Applicant: KAO CORP

    Inventor： ARA KATSUTOSHI ,  SAEKI KATSUHISA ,  IGARASHI KAZUAKI ,  ITO SUSUMU

    IPC: C12P19/14 ,  C12N9/44 ,  C12P19/16 ,  C12P19/20 ,  C12P19/22 ,  C12R1/07

    Abstract: PURPOSE:To carry out the saccharification of a polysaccharide at a relatively low temperature under alkaline condition in high efficiency by treating a polysaccharide with an alkali pullulanase having amylase activity and produced by a bacterial strain belonging to genus Bacillus. CONSTITUTION:A polysaccharide is saccharified with alkali pullulanase Y having alpha-amylase activity and produced by a bacterial strain belonging to genus Bacillus. The polysaccharide is preferably starch, soluble starch, etc. The bacterial strain used in the present process is a bacillus having a cell size of (0.8-2.4)X(1.8-4.0)mum and bacteriological properties such as locomotivity and alkalophilic property, e.g. FERM 10886.

    CLEANSER COMPOSITION
    95.
    发明专利
    CLEANSER COMPOSITION 失效

    公开(公告)号：JPH03290498A

    公开(公告)日：1991-12-20

    申请号：JP9156390

    申请日：1990-04-06

    Applicant: KAO CORP

    Inventor： SONE TAEKO ,  TOSAKA MASAKI ,  IGARASHI KAZUAKI ,  ARA KATSUTOSHI ,  DEGUCHI KATSUHIKO

    IPC: C12N9/44 ,  C11D3/386 ,  C12N1/20 ,  C12R1/07

    Abstract: PURPOSE:To provide a cleanser composition effectively acting on the starch stains strongly adhered to table wares, fibers, etc., and having a remarkably improved cleanability by compounding an alkali pullulanase or alkali-resistant pullulanase having an alpha-amylase activity. CONSTITUTION:The objective composition comprises an alkali pullulanase or alkali-resistant pullulanase having an alpha-amylase activity in an amount of usually 0.1-10wt.%, the pullulanase being produced by Bacillus sp. KSM-AP1378 (FERM 10886). The purified enzyme may be used as the alkali or alkali-resistant pullulanase and a culture solution may be used as such as the crude enzyme.

    Polypeptide and recombinant microorganism
    97.
    发明专利
    Polypeptide and recombinant microorganism 有权
    多肽和重组微生物

    公开(公告)号：JP2013066434A

    公开(公告)日：2013-04-18

    申请号：JP2011207792

    申请日：2011-09-22

    Applicant: Kao Corp ,  花王株式会社

    Inventor： KAKESHITA HIROSHI ,  KAGEYAMA YASUSHI ,  ARA KATSUTOSHI

    IPC: C12N15/09 ,  C07K14/555 ,  C07K19/00 ,  C12N1/21 ,  C12P21/02

    Abstract: PROBLEM TO BE SOLVED: To provide a polypeptide which has an excellent secretory property and to provide a recombinant microorganism.SOLUTION: The polypeptide is produced by joining (A) amino acid sequence to the N-terminus side in the amino acid sequence of a target protein or polypeptide other than the AmyE protein of Bacillus subtilis, and a recombinant microorganism is provided by introducing the base sequence encoding the amino acid sequence of the produced polypeptide to a host microorganism. The amino acid sequence is all or a consecutive part of the amino acid sequence shown in (A) sequence number 1, including at least the region of the first to 44th positions.

    Abstract translation: 待解决的问题：提供具有优异分泌性质并提供重组微生物的多肽。 解决方案：通过将（A）氨基酸序列连接到除枯草芽孢杆菌的AmyE蛋白以外的靶蛋白或多肽的氨基酸序列中的N末端侧产生多肽，并且重组微生物由 将编码产生的多肽的氨基酸序列的碱基序列引入宿主微生物。 氨基酸序列是（A）序列号1所示的氨基酸序列的全部或连续部分，包括至少第一至第44位的区域。 版权所有（C）2013，JPO＆INPIT

    Gene expression method
    99.
    发明专利
    Gene expression method 有权
    基因表达方法

    公开(公告)号：JP2012019721A

    公开(公告)日：2012-02-02

    申请号：JP2010159155

    申请日：2010-07-13

    Applicant: Kao Corp ,  花王株式会社

    Inventor： MANABE KENJI ,  KAGEYAMA YASUSHI ,  ARA KATSUTOSHI

    IPC: C12N15/09 ,  C12N1/21 ,  C12N9/42 ,  C12P21/02

    CPC classification number: Y02P20/52

    Abstract: PROBLEM TO BE SOLVED: To provide a new gene expression method, a protein or polypeptide production method using the new gene expression method, and recombinant Bacillus subtilis used for the methods, especially, to provide a gene expression method and a protein or polypeptide production method using the gene expression method which are more efficient and simpler than the conventional methods, and to provide recombinant Bacillus subtilis used for the methods.SOLUTION: Bacillus subtilis is used as a host, and the recombinant Bacillus subtilis in which the amount of expression of the rocG gene in the genome is controlled according to culture phases is used. The method by which the amount of expressions of a target gene is efficiently controlled is provided.

    Abstract translation: 要解决的问题：提供新的基因表达方法，使用新的基因表达方法的蛋白质或多肽的制备方法和用于该方法的重组枯草芽孢杆菌，特别是提供基因表达方法和蛋白质或 使用比常规方法更有效和更简单的基因表达方法的多肽生产方法，并提供用于该方法的重组枯草芽孢杆菌。 使用枯草芽孢杆菌作为宿主，使用其中根据培养期控制基因组中的rocG基因的表达量的重组枯草芽孢杆菌。 提供了靶基因的表达量有效控制的方法。 版权所有（C）2012，JPO＆INPIT

    New variant of bacillus subtilis
    100.
    发明专利
    New variant of bacillus subtilis 有权
    BACILLUS SUBTILIS的新变种

    公开(公告)号：JP2011160686A

    公开(公告)日：2011-08-25

    申请号：JP2010024571

    申请日：2010-02-05

    Applicant: Kao Corp ,  Nara Institute Of Science & Technology ,  国立大学法人 奈良先端科学技術大学院大学 ,  花王株式会社

    Inventor： MORIMOTO TAKUYA ,  KAGEYAMA YASUSHI ,  ARA KATSUTOSHI ,  OGASAWARA NAOKI

    IPC: C12N1/21 ,  C12N15/09 ,  C12P21/02

    Abstract: PROBLEM TO BE SOLVED: To provide a variant of Bacillus subtilis from which a large region of genome is deleted based on a wild strain, and to provide a method for producing a gene product by using the variant.
    SOLUTION: The variant of the Bacillus subtilis has a genome structure obtained by deleting any one of the following regions in the genome of Bacillus subtilis 168 strain from the genome region of MGB874 strain of the variant of the Bacillus subtilis: (a) ybbU-ybfI region; (b) ydjM-cotA region; (c) yefA-yesX region; (d) yfiB-yfiX region; (e) yhcE-yhcU region; (f) yhaU-yhaL region; (g) yjbX-yjlB region; (h) xkdA-ykcC region; (i) bpr-ylmA region; (j) flgB-cheD region; (k) ynfF-ppsA region; (l) yoxC-yobO region; (m) spoVAF-spoIIAA region; (n) spoIIIAH-yqhV region; (o) ytvB-ytqB region; (p) yteA-ytaB region; (q) yuaJ-yugO region; (r) yusJ-mrgA region; (s) gerAA-yvrI region; (t) yvaM-yvbK region; (u) araE-yveK region; (v) yvdE-yvcP region; (w) gerBA-ywsC region; (x) ywrK-ywqM region; (y) spoIIID-ywoB region; (z) slp-ylaF region; (aa) licH-sigY region; (ab) yqeF-yrhK region; (ac) yuzE-yukJ region; and (ad) yncM-yndN region.
    COPYRIGHT: (C)2011,JPO&INPIT

    Abstract translation: 待解决的问题：提供一种枯草芽孢杆菌的变体，其中基于野生株的基因组的大区域被缺失，并提供通过使用该变体产生基因产物的方法。 解决方案：枯草芽孢杆菌的变体具有通过删除来自枯草芽孢杆菌变体的MGB874菌株的基因组区域的枯草芽孢杆菌168菌株的基因组中的以下区域中的任一个获得的基因组结构：（a） ybbU-ybfI区域 （b）ydjM-cotA区域; （c）yefA-yesX地区; （d）yfiB-yfiX区域; （e）yhcE-yhcU区域; （f）yhau-yhaL地区; （g）yjbX-yjlB区域; （h）xkdA-ykcC区域; （i）bpr-ylmA区; （j）flgB-cheD区域; （k）ynfF-ppsA区域; （l）yoxC-yobO区; （m）spoVAF-spoIIAA区域; （n）spoIIIAH-yqhV区域; （o）ytvB-ytqB区域; （p）yteA-ytaB区域; （q）yuaJ-yugO地区; （r）yusJ-mrgA区域; （s）gerAA-yvrI区域; （t）yvaM-yvbK区域; （u）araE-yveK区域; （v）yvdE-yvcP区; （w）gerBA-ywsC区域; （x）ywrK-ywqM区域; （y）spoIIID-ywoB区域; （z）slp-ylaF区; （aa）licH-sigY区域; （ab）yqeF-yrhK区域; （ac）yuzE-yukJ地区; 和（ad）yncM-yndN区​​域。 版权所有（C）2011，JPO＆INPIT

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