公开(公告)号：WO2009119862A3

公开(公告)日：2009-12-10

申请号：PCT/JP2009056409

申请日：2009-03-23

Applicant: KAO CORP ,  NARA INST SCIENCE & TECHNOLOGY ,  ARA KATSUTOSHI ,  MORIMOTO TAKUYA ,  OGASAWARA NAOTAKE

Inventor： ARA KATSUTOSHI ,  MORIMOTO TAKUYA ,  OGASAWARA NAOTAKE

IPC: C12N15/09

CPC classification number: C12N15/1082 ,  C12N15/102

Abstract: A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5'-side region outside of the target region in the host DNA, a 3'-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3'-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5'-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performing homologous recombination, within the host DNA integrated with the donor DNA by the first step, between two regions of the 3'-side region derived from the host DNA and the 3'-side region derived from the donor DNA, to conduct selection of a host whose target region is modified based on expression of the second selectable marker gene under an expression-inducing condition for the expression-inducing promoter: and a selectable marker cassette for use in the method.

Abstract translation: 使用供体DNA修饰宿主DNA中的目标区域的方法：其中供体DNA具有与宿主DNA中靶区域外侧的5'-侧区域同源的区域，靶外侧的3'-侧区域 宿主DNA中的第一同源重组区域和宿主DNA中的目标区域内的第一同源重组区域，并且还具有第一选择标记基因，表达诱导启动子和第二选择标记基因，所述第二选择标记基因在 控制与3'侧区域同源的区域和与第一同源重组区域同源的区域之间的表达诱导型启动子; 该方法具有以下步骤：第一步，在供体DNA和宿主DNA之间的5'侧区域和第一同源重组区域的区域进行同源重组，以选择与供体DNA整合的宿主 基于第一选择标记基因的表达; 以及第二步骤，其在通过第一步骤在宿主DNA整合的宿主DNA内进行同源重组，所述宿主DNA源自宿主DNA的3'侧区域和源自供体DNA的3'侧区域的两个区域之间 在表达诱导启动子的表达诱导条件下，基于第二选择标记基因的表达来选择靶区被修饰的宿主;以及用于该方法中的选择标记盒。

公开(公告)号：EP2133416A4

公开(公告)日：2010-11-10

申请号：EP07859666

申请日：2007-12-27

Applicant: KAO CORP

Inventor： ENDO KEIJI ,  ARA KATSUTOSHI

IPC: C12N1/21 ,  C12N15/09 ,  C12P21/02

CPC classification number: C12N15/75 ,  C07K14/32 ,  C12P21/02

公开(公告)号：EP1944365A4

公开(公告)日：2009-01-14

申请号：EP06798311

申请日：2006-09-25

Applicant: KAO CORP ,  NARA INST SCIENCE & TECHNOLOGY

Inventor： KADOYA RYOSUKE ,  ENDO KEIJI ,  TOHATA MASATOSHI ,  ARA KATSUTOSHI ,  OGASAWARA NAOTAKE

IPC: C12N15/09 ,  C12N1/21

CPC classification number: C12N15/75 ,  C12N9/2417 ,  C12N9/54 ,  C12P21/02

Abstract: Analysis is made comprehensively on gene-disrupted strains derived from Bacillus subtilis to discover a novel Bacillus subtilis mutant strain which is excellent in the ability of producing various enzymes. The Bacillus subtilis mutant strain has a genomic structure having the deletion of any one of the regions shown in the deleted region list. The Bacillus subtilis mutant strain shows a significantly improved ability of secreting and producing a desired secreted protein when a gene encoding the protein is expressably introduced into the strain, compared to a case where the gene is introduced into a wild-type strain.

公开(公告)号：EP2088192A4

公开(公告)日：2012-07-04

申请号：EP07830247

申请日：2007-10-16

Applicant: KAO CORP ,  UNIV SHINSHU

Inventor： ARA KATSUTOSHI ,  SEKIGUCHI JUNICHI ,  YAMAMOTO HIROKI ,  HARADA HIROYUKI

IPC: C12N9/99 ,  C07K14/32 ,  C12N1/04 ,  C12N1/06 ,  C12N1/21 ,  C12N15/09 ,  C12P21/00

CPC classification number: C07K14/32 ,  C12P21/02

公开(公告)号：EP1985695A4

公开(公告)日：2010-03-03

申请号：EP07706337

申请日：2007-02-15

Applicant: KAO CORP

Inventor： ENDO KEIJI ,  LIU SHENGHAO ,  ARA KATSUTOSHI

IPC: C12N1/21 ,  C12N9/42 ,  C12N15/09

CPC classification number: C12N9/2437

公开(公告)号：EP1616559A4

公开(公告)日：2007-01-17

申请号：EP03816611

申请日：2003-09-19

Applicant: KAO CORP

Inventor： AKIBA SYUNICHI ,  ARA KATSUTOSHI ,  KUSUOKU HIROSHI ,  HAGURA TOYOKI

IPC: A61K8/00 ,  A61K8/97 ,  A61K36/16 ,  A61K36/756 ,  A61Q15/00

CPC classification number: A61K36/756 ,  A61K8/97 ,  A61K36/16 ,  A61Q15/00 ,  A61K2300/00

Abstract: This invention relates to deodorant agents, which contain ginkgo or Phellodendron Bark or its extract as an active ingredient. According to the present invention, deodorant agents which are high in safety and can radically inhibit the emission of human body malodors typified by sweat odor, especially, axillary odor.

公开(公告)号：EP0670367A4

公开(公告)日：2000-01-05

申请号：EP94915270

申请日：1994-05-19

Applicant: KAO CORP

Inventor： ARA KATSUTOSHI ,  SAEKI KATSUHISA ,  IGARASHI KAZUAKI ,  TAKAIWA MIKIO ,  UEMURA TAKAAKI ,  KAWAI SHUJI ,  ITO SUSUMU ,  HAGIWARA HIROSHI ,  KOBAYASHI TOHRU ,  TANAKA ATSUSHI ,  HOSHINO EIICHI

IPC: C11D3/386 ,  C12N9/28

CPC classification number: C12N9/2417 ,  C11D3/386

Abstract: A liquefying alkaline α-amylase having the following enzymological properties: (1) action: this enzyme hydrolyzes α-1,4-glycoside linkages of starch, amylose, amylopectin and partial hydrolyzates thereof, and forms glucose, maltose, maltotriose, maltotetraose, maltopentaose and maltohexaose from amylose, though it does not act upon pullulan; (2) isoelectric point: it has an isolectric point exceeding 8.5 as measured by isoelectric electrophoresis. This enzyme has such a liquefying activity as to hydrolyze starch and starch polysaccharides at high random and has an optimum pH value on an alkaline side. Since it has a high isoelectric point, it can readily be purified. A detergent composition containing this enzyme has an excellent detergency against especially stains due to spilt food.

公开(公告)号：DK2451954T3

公开(公告)日：2018-11-05

申请号：DK10773723

申请日：2010-10-18

Applicant: KAO CORP

Inventor： KAWAHARA AKIHITO ,  KODAMA HIROSHI ,  ARA KATSUTOSHI

IPC: C12N15/113

公开(公告)号：AU2008238982B2

公开(公告)日：2013-02-07

申请号：AU2008238982

申请日：2008-04-08

Applicant: KAO CORP

Inventor： ARA KATSUTOSHI ,  LIU SHENGHAO ,  ENDO KEIJI

IPC: C12N1/20 ,  C12R1/125

Abstract: A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided. A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of Bacillus subtilis or a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.

公开(公告)号：DK1944365T3

公开(公告)日：2012-04-30

申请号：DK06798311

申请日：2006-09-25

Applicant: KAO CORP ,  NARA INST SCIENCE & TECHNOLOGY

Inventor： ENDO KEIJI ,  TOHATA MASATOSHI ,  ARA KATSUTOSHI ,  OGASAWARA NAOTAKE ,  KADOYA RYOSUKE

IPC: C12N15/09 ,  C12N1/21