公开(公告)号：WO1990011687A1

公开(公告)日：1990-10-18

申请号：PCT/US1990001573

申请日：1990-03-23

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  CURTISS, Roy, III ,  GALAN, Jorge

IPC: A01N63/00

CPC classification number: C12R1/42 ,  A61K39/0275 ,  A61K2039/522 ,  C07K14/255 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/40 ,  C12N9/0008 ,  C12N9/16 ,  C12N15/625 ,  C12N15/65 ,  C12N15/74 ,  C12Y102/01011 ,  Y02A50/482

Abstract: The phoP gene and its equivalents are of a type which have ''global regulation of pathogenicity'', i.e., they coordinately regulate a number of genes including those that encode bacterial virulence factors. In Salmonella, the phoP gene product also controls the expression of non-specific acid phosphatase from the phoN gene. A central feature of the invention are microorganisms which are avirulent as a result, in whole or in part, of a mutation in phoP, but which retain their immunogenicity. These cells are suitable as components of live vaccines.

Abstract translation: phoP基因及其等同物是具有“致病性的全局调节”的类型，即它们协调调节许多基因，包括编码细菌毒力因子的基因。 在沙门氏菌中，phoP基因产物也控制来自phoN基因的非特异性酸性磷酸酶的表达。 本发明的中心特征是微生物，其全部或部分无光毒性，其在phoP中的突变，但保留其免疫原性。 这些细胞适合作为活疫苗的组分。

公开(公告)号：WO1990009800A1

公开(公告)日：1990-09-07

申请号：PCT/US1990001037

申请日：1990-02-20

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  BOIME, Irving ,  MATZUK, Martin, M. ,  KEENE, Jeffrey, L.

IPC: C12P21/00

CPC classification number: C07K14/59 ,  A61K38/00 ,  C07K2319/00 ,  C07K2319/75 ,  C07K2319/91

Abstract: The invention provides recombinant native and mutein forms of human reproductive hormones with characteristic glycosylation patterns which are influential in the metabolic activity of the protein. The invention also provides recombinant mutant forms of the human alpha subunit common to FSH, LH, CG, and TSH, to obtain hormones which also have unique glycosylation patterns. Also provided are recombinant materials to produce these subunits separately or together to obtain complete heterodimeric hormones of regulated glycosylation pattern and activity. Modified forms of LH and FSH beta subunits which enhance the rate of dimerization and secretion of the dimers or individual chains are also disclosed.

Abstract translation: 本发明提供具有特征性糖基化模式的人类生殖激素的重组天然和突变蛋白形式，其在蛋白质的代谢活性中是有影响的。 本发明还提供FSH，LH，CG和TSH共同的人α亚基的重组突变体形式，以获得也具有独特糖基化模式的激素。 还提供了分别或一起产生这些亚单位以获得调节的糖基化模式和活性的完全异二聚体激素的重组材料。 还公开了增强二聚体或单链的二聚化和分泌速率的LH和FSHβ亚基的修饰形式。

公开(公告)号：WO1989007257A1

公开(公告)日：1989-08-10

申请号：PCT/US1989000328

申请日：1989-01-26

Applicant: WASHINGTON UNIVERSITY ,  BINNS, Robert, W. ,  EPSTEIN, John, W. ,  ISRAEL, Martin, H. ,  KLARMANN, Joseph ,  WONG, John, Wai-Chu

Inventor： WASHINGTON UNIVERSITY

IPC: G01N23/04

CPC classification number: G02B6/04 ,  G01N23/043 ,  H05G1/64

Abstract: Apparatus for generating a reduced-scale optical image of an x-ray beam pattern or the like. The apparatus includes an optical fiber assembly (38) which forms a two-dimensional input array and whose output ends form a reduced-scale two-dimensional output array. A beam converter (34, 36) in the apparatus converts the irradiation beam (29) to a corresponding electron beam, and this beam is converted to a two-dimensional light image of the beam which is directed onto the input array. In one embodiment, the optical fibers form a fiber assembly (38) constructed of an array of multifiber reducers which are tapered on progressing between input and output ends.

Abstract translation: 用于产生X射线束图案等的缩小尺度光学图像的装置。 该装置包括形成二维输入阵列的光纤组件（38），其输出端形成缩小的二维输出阵列。 装置中的光束转换器（34,36）将照射光束（29）转换成相应的电子束，并将该光束转换成被引导到输入阵列上的光束的二维光图像。 在一个实施例中，光纤形成由多光纤减速器阵列构成的光纤组件（38），其在输入端和输出端之间逐渐变细。

公开(公告)号：WO1989003057A1

公开(公告)日：1989-04-06

申请号：PCT/US1988003161

申请日：1988-09-20

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  LICHTMAN, Jeffrey, W. ,  SUNDERLAND, William, J.

IPC: G02B21/00

CPC classification number: G02B21/0084 ,  G02B21/0028 ,  G02B21/0032 ,  G02B21/0044 ,  G02B21/082

Abstract: A microscope design for, and a kit to convert a standard epi-illumination microscope into a single aperture confocal scanning epi-illumination microscope comprises an assembly including, in one embodiment, a pair of intermediate lenses (28, 32) to create a second field plane (70) conjugate to the specimen plane (68) in both the incident and reflected light paths, with a single aperture positioned at this second conjugate field plane and controllably scanned through the plane to create the incident light beam as well as to mask the returning light before viewing. In a second embodiment, only a single lens (128) is included in the assembly and the objective lens may be positioned at its correct tube length, or a non-standard objective lens (152) may be used to prevent undesired degradation of the image. This assembly conveniently mounts to the nosepiece of the standard microscope in place of the objective lens, and the objective lens mounts to the assembly.

Abstract translation: 用于将标准epi照明显微镜转换成单孔共聚焦扫描外照相显微镜的显微镜设计和套件包括在一个实施例中包括一对中间透镜（28,32）以组合第二场 平面（70）在入射和反射光路两者中与样本平面（68）共轭，单个孔位于该第二共轭场平面，并可控地扫描通过平面以产生入射光束以及掩蔽 在观看前返回光线。 在第二实施例中，在组件中仅包括单个透镜（128），并且物镜可以被定位在其正确的管长度，或者可以使用非标准物镜（152）来防止图像的不期望的劣化 。 该组件方便地安装在标准显微镜的鼻梁上代替物镜，物镜安装到组件上。

公开(公告)号：WO1998025970A1

公开(公告)日：1998-06-18

申请号：PCT/US1997022724

申请日：1997-12-10

Applicant: WASHINGTON UNIVERSITY ,  SANTORO, Samuel, A. ,  STAATZ, William, D.

Inventor： WASHINGTON UNIVERSITY

IPC: C07K14/78

CPC classification number: A61K38/10 ,  C07K14/78

Abstract: Novel short peptides are disclosed which are selected from the group consisting of TAVTQTYGGNSNGEP and fragments thereof containing the minimal sequence TATQTY. These peptides are antithrombotic agents and mediate divalent cation-independent adhesion of platelets to fibronectin.

Abstract translation: 公开了新的短肽，其选自TAVTQTYGGNSNGEP及其含有最小序列TATQTY的片段。 这些肽是抗血栓形成剂，并介导血小板与纤连蛋白的二价阳离子非依赖性粘附。

公开(公告)号：WO1998010290A1

公开(公告)日：1998-03-12

申请号：PCT/US1997015359

申请日：1997-08-29

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  ROTH, Kevin, A. ,  LORENZ, Robinna

IPC: G01N33/567

CPC classification number: G01N33/535

Abstract: A method for localizing and quantitating a target substance in a biological sample is disclosed. The method utilizes an enzyme-linked probe that binds to the target substance and generates a depositable photoluminescent substance which detects position and a soluble photoluminescent substance which allows quantitation in the medium bathing the sample.

Abstract translation: 公开了一种用于定位和定量生物样品中的目标物质的方法。 该方法利用与目标物质结合的酶联探针，并产生检测位置的可沉积光致发光物质和允许在洗涤样品的培养基中定量的可溶性光致发光物质。

公开(公告)号：WO1998009134A1

公开(公告)日：1998-03-05

申请号：PCT/US1997015267

申请日：1997-08-29

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  LICHTMAN, Jeffrey, W. ,  CONCHELLO, Jose-Angel

IPC: G01B11/14

CPC classification number: G01B11/24 ,  G02B21/22

Abstract: A method and apparatus for generating an image of a specimen comprising dividing the in-focus plane of an objective of an optical apparatus (20) into a plurality of grid regions (45). Incident light (40) is directed to the grid regions (45) to illuminate surface portions of the specimen (22) that are within the grid regions (45). Light reflected from the surface portions is sensed to determine approximate spatial slopes of each surface portion associated with each grid region. The spatial slopes are integrated, in a processor (38), to generate a topographical map representative of surface portion of the specimen that are within the depth of focus of the objective. Consecutive planes can be obtained to generate topographical maps thicker than the depth of focus of the objective.

Abstract translation: 一种用于产生样本图像的方法和装置，包括将光学装置（20）的物镜的聚焦平面分成多个格栅区域（45）。 入射光（40）被引导到格栅区域（45）以照亮在格栅区域（45）内的样本（22）的表面部分。 感测从表面部分反射的光以确定与每个格栅区域相关联的每个表面部分的近似空间斜率。 空间斜率在处理器（38）中被积分，以产生代表在物镜的焦深范围内的样本表面部分的地形图。 可以获得连续的平面以产生比物镜的焦深更厚的地形图。

公开(公告)号：WO1998008114A1

公开(公告)日：1998-02-26

申请号：PCT/US1997014588

申请日：1997-08-19

Applicant: MCDONNELL DOUGLAS CORPORATION ,  WASHINGTON UNIVERSITY ,  SYNDERMAN, David, M. ,  PALMER, Donald, D., Jr. ,  CONRADI, Mark, S. ,  LEOPOLD, Daniel, J. ,  FRY, Charles, G.

Inventor： MCDONNELL DOUGLAS CORPORATION ,  WASHINGTON UNIVERSITY

IPC: G01V03/00

CPC classification number: G01R33/4641 ,  G01R33/4625

Abstract: A method using nuclear magnetic resonance techniques to detect corrosion within metallic structures such as aluminum under non-ferromagnetic coatings without first having to remove the coatings. The metallic structure is placed within an external static magnetic field. A series of radio frequency preparation pulses (34) and inspection pulses (36) is applied to the metallic structure, for exciting and disturbing the equilibrium orientation of the hydrogen nuclei within the metallic structure with the preparation pulses (34), and for generating a series of nuclear magnetic resonance signals (38) with the inspection pulses (36). The response signals (38) are detected and the amplitude of the response signals (38) is compared with the spin-spin relaxation time of corrosion for detecting the presence of corrosion in the structure.

Abstract translation: 一种使用核磁共振技术检测非铁磁性涂层等金属结构如铝的腐蚀的方法，而无需先除去涂层。 金属结构放置在外部静磁场内。 一系列射频准备脉冲（34）和检查脉冲（36）被施加到金属结构，用于利用制备脉冲（34）激发和干扰金属结构内的氢核的平衡取向，并且用于产生 一系列具有检查脉冲（36）的核磁共振信号（38）。 检测响应信号（38），并将响应信号（38）的振幅与用于检测结构中腐蚀的存在的腐蚀的自旋 - 自旋弛豫时间进行比较。

公开(公告)号：WO1998007439A1

公开(公告)日：1998-02-26

申请号：PCT/US1997014862

申请日：1997-08-22

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  McDANIEL, Michael, L. ,  HILL, Jeanette, R. ,  CORBETT, John, A.

IPC: A61K38/00

CPC classification number: A61K31/155

Abstract: A method for treating an individual afflicted with a disease condition mediated by IL-1 bioactivity is disclosed. The method comprises administering an effective amount of the NOS inhibitor, aminoguanidine, to an individual.

Abstract translation: 公开了一种治疗患有IL-1生物活性介导的疾病状况的个体的方法。 该方法包括向个体施用有效量的NOS抑制剂氨基胍。

公开(公告)号：WO1997038133A1

公开(公告)日：1997-10-16

申请号：PCT/US1997004683

申请日：1997-03-20

Applicant: UNIVERSITY OF SOUTH FLORIDA ,  WASHINGTON UNIVERSITY ,  THE INSTITUTE OF GENOMIC RESEARCH ,  HARDY, John ,  GOATE, Alison, M. ,  FULDNER, Rebecca, A.

Inventor： UNIVERSITY OF SOUTH FLORIDA ,  WASHINGTON UNIVERSITY ,  THE INSTITUTE OF GENOMIC RESEARCH

IPC: C12Q01/68

CPC classification number: C12Q1/6883 ,  C07K14/4711 ,  C12Q2600/156

Abstract: Variant presenilin-2 genes are provided. Methods of using these genes in diagnosing Alzheimer's disease are also provided.

Abstract translation: 提供了变异早老素-2基因。 还提供了使用这些基因诊断阿尔茨海默氏病的方法。