公开(公告)号：WO1998010295A1

公开(公告)日：1998-03-12

申请号：PCT/US1997015115

申请日：1997-09-03

Applicant: WASHINGTON UNIVERSITY ,  HEINECKE, Jay, W.

Inventor： WASHINGTON UNIVERSITY

IPC: G01N33/68

CPC classification number: G01N33/6893 ,  G01N2800/323 ,  G01N2800/52 ,  Y10S436/811

Abstract: A diagnostic method and screening test for atherosclerosis and analogous diseases involving activated phagocytes and/or inflammation is provided which comprises determining the presence of p-hydroxyphenylacetaldehyde-lysine in a test sample of a body fluid or tissue at a level which is elevated relative to the level in a normal patient.

Abstract translation: 提供了一种用于动脉粥样硬化和涉及活化的吞噬细胞和/或炎症的类似疾病的诊断方法和筛选试验，其包括测定体液或组织的测试样品中对羟基苯乙醛 - 赖氨酸的存在，其水平相对于 水平在正常的病人。

公开(公告)号：WO1997033601A1

公开(公告)日：1997-09-18

申请号：PCT/US1997004298

申请日：1997-03-17

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  SHAW, Andrey, S. ,  MUSLIN, Anthony, J.

IPC: A61K38/00

CPC classification number: C12N9/1205 ,  A61K38/00

Abstract: Compositions and methods for inhibition of the intracellular activation of a signal transducing protein comprising phosphoserine containing peptides derived from the Raf-1 protein are disclosed. The peptides bind to 14-3-3 protein and thereby prevent its association with the signal transducing peptide to block signal transduction. Also provided is an assay method for identifying a pharmacologic agent that can bind 14-3-3 protein and block signal transduction.

Abstract translation: 公开了用于抑制包含衍生自Raf-1蛋白的含磷酸丝氨酸肽的信号转导蛋白的细胞内活化的组合物和方法。 肽与14-3-3蛋白结合，从而阻止其与信号转导肽的结合阻断信号转导。 还提供了用于鉴定能结合14-3-3蛋白和阻断信号转导的药理学试剂的测定方法。

公开(公告)号：WO1996041158A1

公开(公告)日：1996-12-19

申请号：PCT/US1996008236

申请日：1996-05-31

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  DIMAROGONAS, Andrew, D.

IPC: G01N29/04

CPC classification number: G01H1/16 ,  G01N3/30 ,  G01N3/32 ,  G01N29/045 ,  G01N29/11 ,  G01N29/12 ,  G01N2203/0055 ,  G01N2203/0073 ,  G01N2203/0688 ,  G01N2291/0235 ,  G01N2291/0258 ,  G01N2291/02827 ,  G01N2291/101 ,  G01N2291/267

Abstract: The integrity of structures may be determined by either one of two methods. In a first method, an impulse of energy is introduced into the structure (20), such as by striking the structure (20), and the induced vibration is measured and the modal damping factor is calculated, the modal damping factor being directly related to the integrity of the structure (20). In a second method, a continous energy input is provided to the structure (20) for inducing a continous vibration in the structure (20). This continous vibration is measured with a transducer (28) and a modal damping factor is calculated with a computer (32). The computer uses an algorithm to estimate the modal damping factor of the structure by calculating a theoretical response of an idealized system from several assumed parameters and varying those parameters.

Abstract translation: 结构的完整性可以通过两种方法之一来确定。 在第一种方法中，例如通过撞击结构（20）将结构（20）中的能量脉冲引入到结构（20）中，并测量感应振动并计算模态阻尼系数，模态阻尼因子与 结构的完整性（20）。 在第二种方法中，向结构（20）提供连续能量输入，用于在结构（20）中引起连续的振动。 用一个换能器（28）测量该连续振动，并用计算机（32）计算模态阻尼系数。 计算机使用一种算法来估计结构的模态阻尼因子，通过从几个假定的参数和改变这些参数计算理想系统的理论响应。

公开(公告)号：WO1996026951A1

公开(公告)日：1996-09-06

申请号：PCT/US1996001939

申请日：1996-02-15

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  OLIVO, Paul, D.

IPC: C07H19/00

CPC classification number: C12N9/0093 ,  C12Q1/04 ,  C12Q1/6897 ,  C12Q1/705 ,  G01N33/5091 ,  G01N2333/90209 ,  G01N2333/924 ,  G01N2333/938

Abstract: A diagnostic assay for detecting the presence of an infectious herpesvirus in a specimen and a genetically engineered cell line for use in such assay are disclosed. The cell line used in the assay expresses a reporter gene only if infectious herpesvirus is present in the specimen. The assay involves inoculating a DNA-transfected cell line with a specimen suspected of containing a herpesvirus, allowing a sufficient period of time for the herpesvirus infectious cycle to proceed, and detecting and quantifying the number of herpesvirus-infected cells to determine the number of infectious herpesvirus virions in the specimen. The cell line is a DNA-transfected cell line susceptible to infection by a herpesvirus which is stably transformed with a chimeric gene comprising a herpesvirus inducible promoter and a gene coding for an enzyme, the expression of the enzyme being dependent upon and quantitatively proportional to the presence of herpesvirus. A kit for such assay is also provided.

Abstract translation: 公开了用于检测样本中的感染性疱疹病毒的存在的诊断测定法和用于这种测定的基因工程细胞系。 只有感染性疱疹病毒存在于样本中时，测定中使用的细胞系才能表达报告基因。 该测定包括用疑似含有疱疹病毒的标本接种DNA转染的细胞系，允许疱疹病毒感染循环的足够的时间进行，并检测和定量疱疹病毒感染细胞的数量以确定感染的数量 样本中的疱疹病毒病毒粒子。 细胞系是由疱疹病毒感染易感染的DNA转染的细胞系，其用包含疱疹病毒诱导型启动子和编码酶的基因的嵌合基因稳定转化，所述酶的表达依赖于和与其成比例 存在疱疹病毒。 还提供了用于这种测定的试剂盒。

公开(公告)号：WO1996017059A2

公开(公告)日：1996-06-06

申请号：PCT/US1995014553

申请日：1995-11-21

Applicant: WASHINGTON UNIVERSITY ,  FRAZIER, William, A. ,  GAO, Ai-Guo

Inventor： WASHINGTON UNIVERSITY

IPC: C12N15/12

CPC classification number: C07K14/78 ,  A61K38/00 ,  C07K14/705

Abstract: A 52 kDa protein is disclosed, which is a receptor for the cell-binding peptide sequences of the cell-binding domain (CBD) of thrombospondin 1 (TS1), namely the 4N1s and 7N3 peptide sequences as well as the 4N1K and 4NK peptides.

Abstract translation: 公开了一种52kDa蛋白，其是血小板反应蛋白1（TS1）的细胞结合结构域（CBD）的细胞结合肽序列的受体，即4N1和7N3肽序列以及4N1K和4NK肽。

公开(公告)号：WO1996009067A1

公开(公告)日：1996-03-28

申请号：PCT/US1994010700

申请日：1994-09-21

Applicant: WASHINGTON UNIVERSITY ,  OSDOBY, Philip, A.

Inventor： WASHINGTON UNIVERSITY

IPC: A61K39/395

CPC classification number: C07K16/28 ,  A61K38/00 ,  G01N33/6893

Abstract: Osteoclast associated antigen (47J) and monoclonal antibodies and methods for detecting and ameliorating osteoporotic disease are disclosed. The monoclonal antibodies are specifically reactive with epitopes present on 47J antigen, which is specific to osteoclasts.

Abstract translation: 公开了破骨细胞相关抗原（47J）和用于检测和改善骨质疏松症的单克隆抗体和方法。 单克隆抗体与存在于47J抗原上的表位具有特异性的活性，其是破骨细胞特异性的。

公开(公告)号：WO1996005224A1

公开(公告)日：1996-02-22

申请号：PCT/US1995009664

申请日：1995-08-01

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  BOIME, Irving

IPC: C07K14/59

CPC classification number: C07K14/59 ,  A61K38/00 ,  C07K14/575 ,  C07K2319/00

Abstract: Single-chain forms of the glycoprotein hormone quartet, at least some members of which are found in most vertebrates, are disclosed. In one embodiment of these single-chain forms, the alpha and beta subunits of the wild-type heterodimers or their variants are covalently linked, optionally through a linker moiety. A drug may further be included within the linker moiety to be targeted to receptors for these hormones. Some of the single-chain forms are agonists and others antagonists of the glycoprotein hormone activity. Another embodiment of the single-chain compounds of the invention comprises two beta subunits of the glycoprotein hormones, which beta subunits are the same or different. These "two- beta " forms are antagonists of glycoprotein hormone activity.

Abstract translation: 公开了在大多数脊椎动物中发现的至少一些成员的糖链蛋白激素四重奏的单链形式。 在这些单链形式的一个实施方案中，野生型异二聚体或其变体的α和β亚基任选地通过连接体部分共价连接。 接头部分中还可以包含药物以靶向这些激素的受体。 一些单链形式是激动剂和糖蛋白激素活性的其它拮抗剂。 本发明的单链化合物的另一个实施方案包含糖蛋白激素的两个β亚基，β亚单位是相同或不同的。 这些“双β”形式是糖蛋白激素活性的拮抗剂。

公开(公告)号：WO1995025536A1

公开(公告)日：1995-09-28

申请号：PCT/US1995003189

申请日：1995-03-20

Applicant: WASHINGTON UNIVERSITY ,  THE JEWISH HOSPITAL OF ST. LOUIS ,  SCHWARTZ, Alan, L. ,  WARSHAWSKY, Ilka ,  BROZE, George, J.

Inventor： WASHINGTON UNIVERSITY ,  THE JEWISH HOSPITAL OF ST. LOUIS

IPC: A61K38/17

CPC classification number: C07K14/705 ,  A61K38/57 ,  A61K2300/00

Abstract: The present invention discloses methods and compositions for inhibiting the hepatic clearance of Tissue Factor Pathway Inhibitor (TFPI).

Abstract translation: 本发明公开了抑制组织因子通路抑制剂（TFPI）肝清除的方法和组合物。

公开(公告)号：WO1994025056A2

公开(公告)日：1994-11-10

申请号：PCT/US1994004762

申请日：1994-04-29

Applicant: WASHINGTON UNIVERSITY ,  KHOURI, Roger, Khalil

Inventor： WASHINGTON UNIVERSITY

IPC: A61K37/02

CPC classification number: A61K38/1858 ,  C07K14/49

Abstract: The present invention provides a noninvasive method for improving collateral circulation to tissue at risk of ischemia or ischemic necrosis. Improved collateral circulation is obtained by applying a growth factor from the PDGF family to the area at or around an occluded blood vessel causing compromised direct circulation to the affected tissue. The present invention further provides a method for anastomosing one or more divided blood vessels using a growth factor from the PDGF family. Preferably, the growth factor is administered locally to the end(s) of the divided blood vessel(s) at or around the area where a single divided blood vessel is attached to tissue of an organ at risk of ischemia.

Abstract translation: 本发明提供了一种用于改善患有局部缺血或缺血性坏死风险的组织的侧支循环的非侵入性方法。 通过将来自PDGF家族的生长因子应用于闭塞血管周围或周围的区域获得改善的侧支循环，从而导致受影响组织的直接循环受损。 本发明还提供了使用来自PDGF家族的生长因子来吻合一个或多个分开的血管的方法。 优选地，将生长因子局部施用于分开的血管的端部或周围，其中单个分开的血管附着到具有局部缺血风险的器官的组织。

公开(公告)号：WO1994011505A1

公开(公告)日：1994-05-26

申请号：PCT/US1993010825

申请日：1993-11-10

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  BORA, Nalini, S. ,  KAPLAN, Henry, J.

IPC: C12N15/12

CPC classification number: C07K14/47

Abstract: A novel pars planitis-specific circulating protein, designated P-36, having a molecular mass of approximately 36 kilodaltons is disclosed. The cDNA gene encoding P-36 has been cloned, and the nucleotide and deduced amino acid sequences have been determined. Vectors and systems for expressing recombinant P-36 are described. Also described are antibodies to P-36 and their use in immunoassays for the protein. A method of diagnosing a patient for pars planitis by determining the presence of P-36 in the bloodstream is disclosed.

Abstract translation: 公开了一种具有约36千道尔顿分子量的新型pars平板炎特异性循环蛋白，命名为P-36。 已经克隆了编码P-36的cDNA基因，并且已经确定了核苷酸和推导的氨基酸序列。 描述了用于表达重组P-36的载体和系统。 还描述了P-36的抗体及其在蛋白质的免疫测定中的用途。 公开了通过确定血液中P-36的存在来诊断患者的平面病毒的方法。