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公开(公告)号：US12097501B2
公开(公告)日：2024-09-24
申请号：US17181966
申请日：2021-02-22
Applicant: Fluidigm Corporation
Inventor： Michael L. Phelan , Christopher J. Kubu , Brian Fowler , Gang Sun , Nikita Patel , Naveen Ramalingam
CPC classification number: B01L7/52 , B01L3/502715 , B01L3/527 , C12Q1/686 , B01L2200/027 , B01L2200/10 , B01L2300/0654 , B01L2300/0672 , B01L2300/0816 , B01L2300/0867 , B01L2300/0887 , B01L2300/1822 , B01L2400/0421 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655
Abstract: Described herein are methods, kits and systems for sample enrichment, multi-step library preparation, sample normalization, detection of sample biomolecules and combinations thereof. Enrichment and multi-step library preparation is described in the context of microfluidic workflows. Sample barcoding methods and kits are described for increasing sample throughput while reducing background in negative samples. Integrated microfluidic devices comprising sample processing unit cells coupled to an array of reaction sites are provided for integrated workflows.
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公开(公告)号：US20220119874A1
公开(公告)日：2022-04-21
申请号：US17480067
申请日：2021-09-20
Applicant: Fluidigm Corporation
Inventor： Megan Anderson , Peilin Chen , Brian Fowler , Robert C. Jones , Fiona Kaper , Ronald Lebofsky , Andrew May
IPC: C12Q1/6855 , C12N15/65 , C12N15/66 , C12N15/10 , C12Q1/686 , C12Q1/6874
Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.
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公开(公告)号：US20210254044A1
公开(公告)日：2021-08-19
申请号：US17065922
申请日：2020-10-08
Applicant: Fluidigm Corporation
Inventor： Sten Linnarsson , Gioele Le Manno , Amit Zeisel
IPC: C12N15/10
Abstract: This invention relates to methods for capturing and encoding nucleic acid from a plurality of single cells. A plurality of solid supports is randomly placed into a plurality of compartments, such that the average number of solid supports per compartment, λ1, is less than 1, wherein each solid support carries (a) a unique identification sequence and (b) a capture moiety. A plurality of single cells is randomly placing into the plurality of compartments, such that the average number of cells per compartment, λ2, is less than 1. These random placement steps may be performed in any order. Nucleic acid is then released from each single cell and captured via the capture moiety, such that nucleic acid from each single cell is tagged with a unique identification sequence.
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公开(公告)号：US20190329253A1
公开(公告)日：2019-10-31
申请号：US16377029
申请日：2019-04-05
Applicant: Fluidigm Corporation
Inventor： Naga Gopi Devaraju , Marc A. Unger
Abstract: A microfluidic system includes a substrate, a set of input ports coupled to the substrate, and a set of output ports coupled to the substrate. The microfluidic system also includes a microfluidic processing system coupled to the substrate and including a plurality of processing sites. The microfluidic processing system is coupled to the set of input ports and the set of output ports. The microfluidic system further includes one or more microfluidic logic devices coupled to the substrate and operable to control at least a portion of the microfluidic processing system.
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公开(公告)号：US20190185929A1
公开(公告)日：2019-06-20
申请号：US16215489
申请日：2018-12-10
Applicant: Fluidigm Corporation
Inventor： Carolyn G. Conant , Tze Howe Charn , Jason A. A. West , Xiaohui Wang
IPC: C12Q1/6874 , B01L3/00 , C12Q1/6844
CPC classification number: C12Q1/6874 , B01L3/5027 , B01L3/502761 , B01L2200/0652 , B01L2300/0609 , B01L2300/0893 , C12Q1/6844 , C12Q2525/179 , C12Q2563/179 , C12Q2565/629
Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.
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Patent Agency Ranking
公开(公告)号：US10106846B2
公开(公告)日：2018-10-23
申请号：US15495695
申请日：2017-04-24
Applicant: Fluidigm Corporation
Inventor： Marc A. Unger , Geoffrey Richard Facer , Barry Clerkson , Christopher G. Cesar , Neil Switz
IPC: G01N21/00 , G01N15/06 , C12Q1/686 , G02B21/36 , G01N21/75 , G01N21/64 , G01N21/84 , G02B21/16 , G01N27/447 , B01L7/00 , B01L3/00
Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
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107.发明授权公开(公告)号：US09644235B2
公开(公告)日：2017-05-09
申请号：US14810388
申请日：2015-07-27
Applicant: Fluidigm Corporation
Inventor： Michael Lucero , Marc Unger
CPC classification number: C12Q1/686 , C12Q1/6804 , C12Q1/6813 , C12Q1/6876 , C12Q2600/118 , G01N33/53 , G01N33/6845 , G01N2333/435 , G01N2458/10
Abstract: The invention provides an assay method for detection and/or quantification of a plurality of nucleic acid or protein targets in a sample. In the method probes are used to associate a detectable tag sequence with each of the selected targets present in the sample. Probes or primers sufficient to identify at least 25, and preferably at least 500, different targets are used. The method involves segregating aliquots of the sample from each other and detecting the tag sequences in each aliquot.
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公开(公告)号：US09371965B2
公开(公告)日：2016-06-21
申请号：US13773518
申请日：2013-02-21
Applicant: Fluidigm Corporation
Inventor： Naga Gopi Devaraju , Marc A. Unger
CPC classification number: B01L3/502738 , B01L3/502707 , B01L3/502715 , B01L2200/14 , B01L2300/023 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/123 , B01L2300/14 , B01L2400/0605 , B01L2400/0633 , B01L2400/0655 , F17D3/00 , G01N35/00871 , G01N2035/00247 , Y10T137/7762
Abstract: A microfluidic device includes an input source characterized by a source pressure and an input channel in fluid communication with the input source. The microfluidic device also includes an output channel and a valve having an open state and a closed state. The valve is disposed between the input channel and the output channel and is characterized by a static pressure. The microfluidic device further includes a control channel coupled to the valve and characterized by a control pressure. In the closed state, the control pressure is greater than atmospheric pressure.
Abstract translation: 微流体装置包括以源压力为特征的输入源和与输入源流体连通的输入通道。 微流体装置还包括输出通道和具有打开状态和关闭状态的阀。 阀设置在输入通道和输出通道之间，其特征在于静压。 微流体装置还包括连接到阀的控制通道，其特征在于控制压力。 在关闭状态下，控制压力大于大气压。
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公开(公告)号：US20160153026A1
公开(公告)日：2016-06-02
申请号：US14880112
申请日：2015-10-09
Applicant: FLUIDIGM CORPORATION
Inventor： Kenneth J. Livak , Marc Unger
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/6834 , C12Q2565/629
Abstract: High throughput methods are used that combine the features of using a matrix-type microfluidic device, labeled nucleic acid probes, and homogenous assays to detect and/or quantify nucleic acid analytes. The high throughput methods are capable of detecting nucleic acid analyses with high PCR and probe specificity, producing a low fluorescence background and therefore, a high signal to noise ratio. Additionally, the high throughput methods are capable of detecting low copy number nucleic acid analyte per cell.
Abstract translation: 使用高通量方法，其结合使用基质型微流体装置，标记的核酸探针和均质测定的特征来检测和/或定量核酸分析物。 高通量方法能够以高PCR和探针特异性检测核酸分析，产生低荧光背景，因此具有高的信噪比。 此外，高通量方法能够检测每个细胞的低拷贝数核酸分析物。
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公开(公告)号：US20160114327A1
公开(公告)日：2016-04-28
申请号：US14864491
申请日：2015-09-24
Applicant: Fluidigm Corporation
Inventor： Jake Kimball , Brandon Ripley , Gang Sun , Dominique Toppani , Myo Thu Maung
IPC: B01L7/00
CPC classification number: B01L7/52 , B01L3/5027 , B01L2200/025 , B01L2200/147 , B01L2300/0819 , B01L2300/0893 , B01L2300/1805 , B01L2300/1822 , C12P19/34 , C12Q3/00 , G01N21/6408 , G01N21/6428 , G01N21/6456 , G01N21/76
Abstract: A thermal cycler for a microfluidic device includes a controller operable to provide a series of electrical signals, a heat sink, and a heating element in thermal communication with the heat sink and operable to receive the series of electrical signals from the controller. The thermal cycler also includes a thermal chuck in thermal communication with the heating element. The thermal chuck comprises a heating surface operable to make thermal contact with the microfluidic device. The heating surface is characterized by a temperature ramp rate between 2.5 degrees Celsius per second and 5.5 degrees Celsius per second and a temperature difference between a first portion of the heating surface supporting a first portion of the microfluidic device and a second portion of the heating surface supporting a second portion of the microfluidic device is less than 0.25° C.
Abstract translation: 用于微流体装置的热循环仪包括控制器，其可操作以提供与散热器热连通的一系列电信号，散热器和加热元件，并可操作以从控制器接收一系列电信号。 热循环仪还包括与加热元件热连通的热卡盘。 热卡盘包括可操作以与微流体装置进行热接触的加热表面。 加热表面的特征在于每秒2.5摄氏度和5.5摄氏度/秒之间的温度升高速率，并且支撑微流体装置的第一部分的加热表面的第一部分与加热表面的第二部分之间的温度差 支撑微流体装置的第二部分小于0.25℃
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