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    SINGLE-CELL TRANSCRIPT SEQUENCING
    2.
    发明申请
    SINGLE-CELL TRANSCRIPT SEQUENCING 审中-公开
    单细胞转录测序

    公开(公告)号：WO2018013723A1

    公开(公告)日：2018-01-18

    申请号：PCT/US2017/041770

    申请日：2017-07-12

    Applicant: FLUIDIGM CORPORATION

    Inventor： LIVAK, Kenneth J. ,  FEKETE, Richard A.

    IPC: C12Q1/68

    Abstract: Described herein are methods for preparing DNA templates for single-cell transcript sequencing of RNA from a population of cells. The methods entail distributing cells from the population into separate reaction volumes so that a plurality of separate reaction volumes each contain a single, isolated cell, wherein the cells have been treated with a fixative prior to distribution. The isolated cells are then permeabilized or disrupted, and cDNA is prepared by reverse transcript, followed by amplification. Also provided is a novel chemistry for efficient production of DNA templates from T-cell receptors or immunoglobulins in single cells.

    Abstract translation: 本文描述了用于制备用于来自细胞群的RNA的单细胞转录物测序的DNA模板的方法。 该方法需要将来自群体的细胞分布到单独的反应体积中，使得多个单独的反应体积各自含有单个分离的细胞，其中细胞在分布之前已经用固定剂处理。 然后使分离的细胞透化或破碎，并通过反转录制备cDNA，然后扩增。 还提供了用于从单细胞中的T细胞受体或免疫球蛋白有效生成DNA模板的新化学物质。

    NUCLEIC ACID ENCODING REACTIONS
    5.
    发明申请
    NUCLEIC ACID ENCODING REACTIONS 审中-公开
    核酸编码反应

    公开(公告)号：WO2012162267A2

    公开(公告)日：2012-11-29

    申请号：PCT/US2012/038894

    申请日：2012-05-21

    Applicant: FLUIDIGM CORPORATION ,  ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  JONES, Robert C. ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    Inventor： ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  JONES, Robert C. ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    IPC: C40B20/00 ,  C40B50/06 ,  C40B40/06 ,  C40B30/04 ,  C12P19/34 ,  C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

    Abstract translation: 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。 在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。 在其它实施方案中，关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中，本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。

    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS
    7.
    发明申请
    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS 审中-公开
    用于靶向靶核酸的多重PCR扩增方法

    公开(公告)号：WO2010115154A1

    公开(公告)日：2010-10-07

    申请号：PCT/US2010/029854

    申请日：2010-04-02

    Applicant: FLUIDIGM CORPORATION ,  MAY, Andrew ,  CHEN, Peilin ,  WANG, Jun ,  KAPER, Fiona ,  ANDERSON, Megan

    Inventor： MAY, Andrew ,  CHEN, Peilin ,  WANG, Jun ,  KAPER, Fiona ,  ANDERSON, Megan

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

    Abstract translation: 在某些实施方案中，本发明提供了其中将核苷酸标签和任选的条形码核苷酸序列加入靶核苷酸序列的扩增方法。 在其它实施例中，本发明提供一种包括多个第一输入线和多个第二输入线的微流体装置。 微流体装置还包括多组第一腔室和多组第二腔室。 每组第一腔室与多个第一输入管线中的一个流体连通。 每组第二腔室与多个第二输入管线中的一个流体连通。 微流体装置还包括与多个第二输入线的第一部分流体连通的多个第一泵元件和与多个第二输入线的第二部分流体连通的多个第二泵元件。

    OPTICAL LENS SYSTEM AND METHOD FOR MICROFLUIDIC DEVICES
    8.
    发明申请
    OPTICAL LENS SYSTEM AND METHOD FOR MICROFLUIDIC DEVICES 审中-公开

    公开(公告)号：WO2005121864A9

    公开(公告)日：2005-12-22

    申请号：PCT/US2005/020250

    申请日：2005-06-07

    Applicant: FLUIDIGM CORPORATION ,  UNGER, Marc, A.

    Inventor： UNGER, Marc, A.

    IPC: H01L27/00 ,  G01T1/10 ,  G01N21/00 ,  G01N21/64 ,  H05B33/00 ,  G01J3/30 ,  C12Q1/00

    Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device (205). The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device (205). The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device (205). The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system (210, 212) coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.

    IMAGE PROCESSING METHOD AND SYSTEM FOR MICROFLUIDIC DEVICES
    9.
    发明申请
    IMAGE PROCESSING METHOD AND SYSTEM FOR MICROFLUIDIC DEVICES 审中-公开
    图像处理方法和微流体装置系统

    公开(公告)号：WO2005011947A2

    公开(公告)日：2005-02-10

    申请号：PCT/US2004/024591

    申请日：2004-07-28

    Applicant: FLUIDIGM CORPORATION ,  TAYLOR, Colin Jon ,  SUN, Gang ,  DUBE, Simant

    Inventor： TAYLOR, Colin Jon ,  SUN, Gang ,  DUBE, Simant

    IPC: B29C

    CPC classification number: G06K9/36 ,  B01L3/5027 ,  G06K9/00 ,  G06K9/00127 ,  G06K9/00134 ,  G06K9/6232 ,  G06T7/0008 ,  G06T7/0012 ,  G06T7/254 ,  G06T7/33 ,  G06T2207/30004

    Abstract: A method for processing an image of a microfluidic device. The method includes receiving a first image of a microfluidic device. The first image corresponds to a first state. Additionally, the method includes receiving a second image of the microfluidic device. The second image corresponds to a second state. Moreover, the method includes transforming the first image and the second image into a third coordinate space. Also, the method includes obtaining a third image based on at least information associated with the transformed first image and the transformed second image, and processing the third image to obtain information associated with the first state and the second state.

    Abstract translation: 一种用于处理微流体装置的图像的方法。 该方法包括接收微流体装置的第一图像。 第一图像对应于第一状态。 另外，该方法包括接收微流体装置的第二图像。 第二图像对应于第二状态。 此外，该方法包括将第一图像和第二图像变换为第三坐标空间。 此外，该方法包括至少基于与变换的第一图像和变换的第二图像相关联的信息获得第三图像，并且处理第三图像以获得与第一状态和第二状态相关联的信息。

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