Abstract:
A photometric reading device comprises a plurality of reading elements (30), each comprising a light emitting diode and a corresponding photodiode on opposing sides of a sample pathway along which a sample microplate (l0) can travel. The microplate (l0) includes rows of sample wells arranged transverse to the line of relative motion of the microplate (l0) and the reading elements (30). The reading elements (30) are arranged such that, during continuous relative motion of the reading elements (30) and the microplate (l0), the reading elements (30) come into registration sequentially with the wells in the first row of wells in the microplate (l0), then with the wells in the second row of wells, and so on.
Abstract:
실시간 PCR(폴리머라아제 연쇄 반응)에서 다수의 목표 화학종의 검출을 위한 기술이 설명되어 있다. 예를 들어, 시스템은 데이터 획득 장치와 이 데이터 획득 장치에 결합된 검출 장치를 포함한다. 검출 장치는 서로 다른 파장의 형광을 발산하는 복수의 화학종을 갖는 복수의 프로세스 챔버를 갖는 회전 디스크를 구비한다. 이 장치는 화학종을 여기하고 서로 다른 파장에서 이 화학종에 의해 발산되는 형광을 포착하도록 광학적으로 구성된 복수의 제거 가능한 광학 모듈을 추가로 구비한다. 복수의 제거 가능한 광학 모듈에 결합된 광섬유 번들은 광학 모듈로부터 단일 검출기로 형광을 전달한다. 또한, 이 장치는 챔버들을 분리하는 밸브의 위치를 찾고 레이저를 이용하여 이 밸브를 가열함으로써 선택적으로 밸브를 개방시켜 디스크 내의 유체의 유동을 제어할 수 있다. 모터, 에너지원, 디스크, 챔버, 검출 장치, 밸브
Abstract:
Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules that are optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector. The device further includes a heating element for heating one or more process chambers on the disk. In addition, the device may control the flow of fluid in the disk by locating and selectively opening valves separating chambers by heating the valves with a laser.
Abstract:
A system for conducting an assay comprises a power source (16), a controller (13) for controlling the assay and a plurality of assay units (14) operatively connected to one another such that the controller can communicate with the assay units and the system is capable of conducting the assay. An assay device comprises a substantially circular body (24) having a plurality of chambers in fluid connection such that fluid can pass between said chambers and a central hub (200) having a sample inlet (202) disposed therein for receiving a sample.
Abstract:
A sample handling system for an optical evaluation instrument. A cuvette storage mechanism (10) is provided for storing a plurality of cuvettes (50), each of which has a plurality of reaction wells. A temperature controlled housing (26) is provided for storing a plurality of reagent containers each containing a respective reagent, and a plurality of sample collection tubes, containing a fluid sample and each presenting an optically scannable code identifying the sample and a test to be performed on the sample. A programming station (30) is provided for optically reading the code (32) presented by the respective sample collection tubes for programming the instrument with a test to be performed. A sample insertion station (14) includes a mechanism (36) for aspirating a portion of the sample from a sample collection tube and for dispensing the aspirated sample portion into a reaction well of a cuvette. A first transporter (28) transports the sample collection tubes first to the programming station (30) and then to the sample insertion station (14). A second transporter (12) transports the cuvettes through the sample insertion station (14) and the reagent station (16, 18 and 20) and onto the monitoring means (22).
Abstract:
Un système (10) utilise un photomètre qui établit un canal optique entre un émetteur de lumière (102) et un détecteur de lumière (106). Le photomètre analyse un spécimen lorsqu'il se trouve dans l'avéole (48) d'un plateau (12). Une plate-forme mobile (74) transporte le plateau (12) vers le canal optique en vue d'une analyse et retire le plateau (12) du canal optique après l'analyse. Le système calibre la plate-forme mobile (74) afin d'assurer un alignement correct entre le spécimen et l'appareil photométrique utilisé pour analyser le spécimen. Le système calibre également le canal optique sans utiliser une référence externe.
Abstract:
On fait passer l'échantillon de sang d'un emplacement in vivo directement dans le récipient d'échantillons au moins partiellement transparent d'un dispositif de prélèvement d'échantillons. Ce récipient d'échantillons comporte une chambre de mesure dans laquelle se trouve un luminophore dont la luminescence est étouffée en présence d'oxygène. On détermine la teneur en oxygène sur la base de la radiation mesurée par un détecteur de radiation. Sont également décrits un dispositif de prélèvement d'échantillons et un système de détermination photométrique in vitro de l'oxygène dans un échantillon de sang.
Abstract:
An analytic instrument of kinetically measuring light absorption characteristics of a plurality of independent samples contained in disposable test tubes arranged in a circular pattern about a single light source. The instrument is designed to be used with a host personal computer and is not specific to any particular type of assay. The instrument obtains and temporarily stores raw data in the form of digitized output signals from the plurality of photodetectors and periodically passes them to the host computer. An incubator has test tube wells arranged in a circular array equidistant from a single incandescent light source. The circular symmetry controls the termal gradients in the incubator such that all of the samples are disposed on the same isotherm and all of the photodetectors are disposed on the same isotherm. The instrument is designed for use with disposable test tubes, and sources of error arising from optical variation in such test tubes are minimized by providing a separate detector for each tube and by holding the tube fixed with respect to the detector. The lignt output from the single light source is continuously monitored and is kept constant.