公开(公告)号：US20230393069A1

公开(公告)日：2023-12-07

申请号：US18448631

申请日：2023-08-11

Applicant: Bio-Rad Laboratories, inc.

Inventor： Darren Roy Link ,  Michael L. Samuels

IPC: G01N21/64 ,  B01J19/00 ,  B01L3/00 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/6851 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/573 ,  G01N33/58

CPC classification number: G01N21/6445 ,  B01J19/0046 ,  B01L3/502761 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/5008 ,  C12Q1/6851 ,  B01F23/41 ,  B01F25/433 ,  B01F25/4336 ,  B01F33/3011 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/573 ,  G01N33/582 ,  B01L7/52

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US11834655B2

公开(公告)日：2023-12-05

申请号：US17565249

申请日：2021-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti

IPC: C12N5/10 ,  C12Q1/6806 ,  C12N15/10 ,  C12Q1/6869 ,  C12Q1/6809

CPC classification number: C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q2563/185 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6855

Abstract: Methods and compositions are provided for making and using uniquely tagged target nucleic acid molecules.

公开(公告)号：US20230364614A1

公开(公告)日：2023-11-16

申请号：US18227212

申请日：2023-07-27

Applicant: Bio-Rad Laboratories, Inc. ,  Bio-Rad Europe GmbH

Inventor： Iago Pereiro Pereiro ,  Anna Fomitcheva Khartchenko ,  Robert Dean Lovchik ,  Govind Kaigala ,  Lorenzo Franco Teodoro Petrini ,  Anel Zulji ,  Frédéric M. Buffière ,  Johann Guégan ,  Eliane Rivalin ,  Sylvie Villard-Saussine

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01L3/502746 ,  B01L2200/027 ,  B01L2300/0832 ,  B01L2400/0487 ,  B01L2400/0622

Abstract: In one implementation, a microfluidic probe has a non-planar processing surface and an inlet aperture. The shape of the surface may be selected to produce a specific velocity gradient profile across a surface onto which fluid is deposited using the microfluidic probe, for example a constant velocity gradient or a velocity gradient that decreases linearly with distance from the inlet aperture. The microfluidic probe may define and overflow notch in a perimeter edge of the processing surface.

公开(公告)号：US11808697B2

公开(公告)日：2023-11-07

申请号：US17877680

申请日：2022-07-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Evan Thrush ,  Steven Swihart ,  William Strong ,  Trey Marlowe ,  Li Lu

IPC: G01N21/31 ,  G01N21/3577 ,  G01N21/3563

CPC classification number: G01N21/3103 ,  G01N21/3563 ,  G01N21/3577 ,  G01N2021/3572 ,  G01N2201/0686

Abstract: Systems for protein quantitation using a Fabry-Perot interferometer. In one arrangement, a quantitation device includes an infrared source, a sample holder, and a Fabry-Perot interferometer positioned to receive infrared radiation from the source passing through a sample on the sample holder. A band pass optical filter sets the working range of the interferometer, and radiation exiting the interferometer falls on a detector that produces a signal indicating the intensity of the received radiation. A controller causes the interferometer to be tuned to a number of different resonance wavelengths and receives the intensity signals, for determination of an absorbance spectrum.

公开(公告)号：US20230314302A1

公开(公告)日：2023-10-05

申请号：US18195084

申请日：2023-05-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  C12M1/00 ,  B01L3/00 ,  B01L3/02 ,  G01N1/28 ,  G01N1/40 ,  G01N1/20

CPC classification number: G01N15/1484 ,  B01L3/021 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  G01N1/4077 ,  G01N2015/149 ,  G01N2035/00158

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US20230314301A1

公开(公告)日：2023-10-05

申请号：US18195048

申请日：2023-05-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  C12M1/00 ,  B01L3/00 ,  B01L3/02 ,  G01N1/28 ,  G01N1/40 ,  G01N1/20

CPC classification number: G01N15/1484 ,  B01L3/021 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  G01N1/4077 ,  G01N2015/149 ,  G01N2035/00158

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US20230313278A1

公开(公告)日：2023-10-05

申请号：US18106189

申请日：2023-02-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY

IPC: C12Q1/6834 ,  C12Q1/6855

CPC classification number: C12Q1/6834 ,  C12Q1/6855 ,  C12Q2565/1015 ,  C12Q2563/137

Abstract: Methods and compositions for attaching cell-specific barcodes without formation of partitions is provided.

公开(公告)号：US11768198B2

公开(公告)日：2023-09-26

申请号：US16795467

申请日：2020-02-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: G01N33/53 ,  G01N33/532 ,  C40B50/08 ,  C12Q1/6874 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12N15/10

CPC classification number: G01N33/53 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/532 ,  G01N33/5436 ,  G01N33/58 ,  C12Q2521/10 ,  C12Q2563/179 ,  C12Q2565/30 ,  G01N2458/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US20230280337A1

公开(公告)日：2023-09-07

申请号：US18305650

申请日：2023-04-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874

CPC classification number: G01N33/54366 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874 ,  B01F33/3011

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US20230264171A1

公开(公告)日：2023-08-24

申请号：US18307991

申请日：2023-04-27

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： CHRISTOPHER BELISLE ,  HONG CHEN ,  YUEPING XU ,  JIALI LIAO ,  XUEMEI HE

IPC: B01J20/26 ,  B01D15/36 ,  B01J20/32 ,  C07K16/06 ,  B01J20/289

CPC classification number: B01J20/262 ,  B01D15/363 ,  B01J20/3285 ,  B01J20/3227 ,  B01J20/3208 ,  B01J20/3219 ,  C07K16/065 ,  B01J20/289 ,  B01J20/3248 ,  B01J20/3253 ,  B01D15/3847

Abstract: Chromatography resins having mixed mode ligands and methods of using such resins are provided.