公开(公告)号：US20240255412A1

公开(公告)日：2024-08-01

申请号：US18633224

申请日：2024-04-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  William Chow

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/01 ,  G01N15/10 ,  G01N15/1433 ,  G01N15/149 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69

CPC classification number: G01N15/1484 ,  B01L3/502715 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  G01N1/4077 ,  G01N15/1433 ,  G01N35/00029 ,  G01N35/1011 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  B01L3/021 ,  B01L3/502746 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  G01N15/01 ,  G01N2015/1006 ,  G01N15/149 ,  G01N2035/00158 ,  G01N2035/1039

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20240253038A1

公开(公告)日：2024-08-01

申请号：US18233819

申请日：2023-08-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: B01L3/00 ,  C12M1/00 ,  C12Q1/6816 ,  C12Q1/6886 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1434 ,  G01N15/149 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574

CPC classification number: B01L3/502761 ,  C12M47/04 ,  C12Q1/6816 ,  G01N15/1434 ,  G01N15/1484 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574 ,  B01L3/502715 ,  B01L3/527 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2400/0406 ,  C12Q1/6886 ,  G01N2015/1006 ,  G01N15/149

Abstract: A system and method, wherein the method includes: (a) receiving a fluid comprising a process reagent for an assay and nucleic acid material from a sample into a fluid pathway coupled to an array of wells, wherein the array of wells is defined within a substrate and each well in the array of wells extends vertically into a planar surface of the substrate; (b) distributing the fluid along the fluid pathway and into wells of the array of wells; and (c) distributing a liquid immiscible with the fluid comprising the process reagent and nucleic acid material along the fluid pathway upon positive pressure generation by a pump of a flow control system in communication with an inlet to the fluid pathway, thereby displacing said fluid along the fluid pathway and into wells of the array of wells and preventing contents of each well from transferring to adjacent wells.

公开(公告)号：US11865542B2

公开(公告)日：2024-01-09

申请号：US17965495

申请日：2022-10-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14 ,  C12Q1/6886 ,  G01N15/10

CPC classification number: B01L3/502761 ,  C12M47/04 ,  C12Q1/6816 ,  G01N15/1434 ,  G01N15/1484 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574 ,  B01L3/502715 ,  B01L3/527 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2400/0406 ,  C12Q1/6886 ,  G01N2015/1006 ,  G01N2015/149

Abstract: A system and method, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity and a fluid delivery module including a fluid pathway through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: generating a set of genetic complexes within individual wells of the array of wells.

公开(公告)号：US11833507B2

公开(公告)日：2023-12-05

申请号：US17590543

申请日：2022-02-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Swati Ranade ,  Vishal Sharma ,  Austin Payne ,  Sam Tuck

IPC: B01L3/00 ,  C12N13/00

CPC classification number: B01L3/502 ,  C12N13/00 ,  B01L2200/026 ,  B01L2300/0893 ,  B01L2400/043

Abstract: A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).

公开(公告)号：US20230338952A1

公开(公告)日：2023-10-26

申请号：US18215054

申请日：2023-06-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: B01L3/00 ,  G01N15/14 ,  G01N1/40 ,  C12M1/00 ,  C12Q1/6888 ,  C12Q1/6813

CPC classification number: B01L3/502715 ,  B01L3/502761 ,  G01N15/1484 ,  G01N1/405 ,  G01N1/4077 ,  C12M47/04 ,  C12Q1/6888 ,  C12Q1/6813 ,  B01L3/502746 ,  B01L2300/0829 ,  G01N2015/1006

Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements (including an integrated imaging subsystem); a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including antibody detection, other protein detection, mRNA detection, and/or other applications associated with spatial transcriptomics.

公开(公告)号：US20230059461A1

公开(公告)日：2023-02-23

申请号：US18047725

申请日：2022-10-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne

IPC: B01L3/00

Abstract: A system and method for receiving and delivering a fluid, the system comprising: a body configured to interface with an opening of a reservoir and defining: a protrusion defining a set position of the body relative to the reservoir; a wall extending from the protrusion; a receiving surface coupled to the wall and sloping from an apex to a nadir along a first direction, the receiving surface comprising a vent; and an outlet positioned closer to the nadir than the apex of the receiving surface and displaced from the vent, the outlet comprising an extension from the body, the extension configured to contact an interior wall of the reservoir, wherein the body comprises: a bubble-mitigating operation mode in which the receiving surface receives and transmits the fluid along the receiving surface, and a fluid-transmitting operation mode in which the body directs the fluid along the interior wall of the reservoir.

公开(公告)号：US20220259644A1

公开(公告)日：2022-08-18

申请号：US17728540

申请日：2022-04-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Yi Zhou

IPC: C12Q1/6841 ,  G01N33/574 ,  C12Q1/6844 ,  C12Q1/686 ,  G01N33/487

Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.

公开(公告)号：US11358146B2

公开(公告)日：2022-06-14

申请号：US17333142

申请日：2021-05-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  C12Q1/6886 ,  G01N15/10 ,  B01L3/00 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14

Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir through which fluid flow is controlled along a fluid path; and wherein the method includes: capturing a population of particles into the array of wells in single-particle format; releasing, from the particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

公开(公告)号：US11231355B2

公开(公告)日：2022-01-25

申请号：US17241897

申请日：2021-04-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  G01N35/00 ,  G01N15/00 ,  G01N15/10 ,  C12M1/00 ,  B01L3/00 ,  B01L3/02 ,  G01N1/28 ,  G01N1/40 ,  G01N1/20 ,  G06K9/00

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US20210283613A1

公开(公告)日：2021-09-16

申请号：US16816817

申请日：2020-03-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne

IPC: B01L3/00

Abstract: A system and method for receiving and delivering a fluid, the system comprising: a body configured to interface with an opening of a reservoir and defining: a protrusion defining a set position of the body relative to the reservoir; a wall extending from the protrusion; a receiving surface coupled to the wall and sloping from an apex to a nadir along a first direction, the receiving surface comprising a vent; and an outlet positioned closer to the nadir than the apex of the receiving surface and displaced from the vent, the outlet comprising an extension from the body, the extension configured to contact an interior wall of the reservoir, wherein the body comprises: a bubble-mitigating operation mode in which the receiving surface receives and transmits the fluid along the receiving surface, and a fluid-transmitting operation mode in which the body directs the fluid along the interior wall of the reservoir.