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公开(公告)号：KR101408985B1
公开(公告)日：2014-06-17
申请号：KR1020120066115
申请日：2012-06-20
Applicant: 건국대학교 산학협력단
Abstract: 본 발명은 베타-글루코시다아제(
β -glucosidase) 효소를 사용하여 다이올계와 트라이올계 희귀 진세노사이드 제조방법에 관한 것으로, 더욱 상세하게는 페니실리움 아큘레툼(
Penicillium
aculeatum ) 유래 베타 글루코시다아제, 및 상기 베타 글루코시다아제의 위치특이적 분해 활성을 이용하여 다이올계와 트라이올계 희귀 진세노사이드를 제조하는 방법에 관한 것이다.-
公开(公告)号：KR1020130126354A
公开(公告)日：2013-11-20
申请号：KR1020120050422
申请日：2012-05-11
Applicant: 건국대학교 산학협력단
CPC classification number: C12P7/6445 , C12N9/88 , C12Y402/01053
Abstract: The present invention relates to a method for producing mono-hydroxy oil and di-hydroxy oil and a composition for the production and, more specifically, to a method for producing mono-hydroxy oil and di-hydroxy oil by a reaction of olive oil and castor oil which are vegetable oil using Lysinibacillusfus formis-derived recombinant oleic acid hydratase; and a composition for production. According to the present invention, the production method using the enzyme is more eco-friendly and has higher specificity than an existing chemical production method. The hydroxy oil obtained through the technique is used as a food additive, an ingredient of cosmetic products, and a natural material of a coating agent, soap, rubber, drugs, and the like and is also used in an emulsifier-free cosmetic product.
Abstract translation: 本发明涉及一羟基油和二羟基油的制备方法和用于生产的组合物，更具体地说，涉及通过橄榄油和二羟基油的反应生产单羟基油和二羟基油的方法 蓖麻油是使用赖氨酸杆菌衍生的重组油酸水合酶的植物油; 和一种用于生产的组合物。 根据本发明，使用酶的生产方法比现有的化学生产方法更环保，具有更高的特异性。 通过该技术获得的羟基油用作食品添加剂，化妆品成分，以及涂料，皂，橡胶，药物等的天然材料，也用于无乳化剂的化妆品中。
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公开(公告)号：KR101304388B1
公开(公告)日：2013-09-11
申请号：KR1020100125085
申请日：2010-12-08
Applicant: 건국대학교 산학협력단
Abstract: 본 발명은 페니실리움 아큘레튬(
Penicillium aculeatum )의 미생물 유래 효소를 효소로 사용하여 희귀 진세노사이드인 F1의 제조방법에 관한 것으로 더욱 상세하게는 자낭균류 곰팡이인 페니실리움 아큘레튬(
Penicillium aculeatum )의 미생물 유래 효소를 사용하여 기질로서 Re, Rg1과 반응용매 중에서 반응시켜 진세노사이드 F1을 제조하는 방법에 관한 것이다.-
公开(公告)号：KR101228975B1
公开(公告)日：2013-02-01
申请号：KR1020100130534
申请日：2010-12-20
Applicant: 건국대학교 산학협력단
Abstract: 본 발명은 엔-아실-디-글루코사민 2-에피머레이즈 (N-acyl-D-glucosamine 2-epimerase) 및 이를 이용하여 포도당(glucose)으로부터 만노스(mannose)를 제조하는 방법에 관한 것으로, 더욱 상세하게는 엔-아실-디-글루코사민 2-에피머레이즈 및 이 효소를 코딩하는 유전자 그리고 이 유전자를 포함하는 재조합 발현 벡터, 이로 형질전환 된 미생물 및 이들을 이용하여 만노스를 얻는 생산 방법에 관한 것이다.
본 발명에 따르면, 종래의 이성화 효소 (isomerase)를 이용하여 과당 (fructose)으로부터 만노스의 생산과는 달리 에피머 효소(epimerae)를 이용하여 과당보다 저가이며 확보에 용이한 포도당으로부터 직접 만노스 생산이 가능하고 기능성 식품 및 의약품 등의 제조시 유용하게 사용될 수 있다.-
公开(公告)号：KR1020130008867A
公开(公告)日：2013-01-23
申请号：KR1020110069515
申请日：2011-07-13
Applicant: 건국대학교 산학협력단
CPC classification number: C12N9/90 , C12P19/02 , C12P19/24 , C12Y503/01008
Abstract: PURPOSE: A mannose-6-phosphate isomerase variant is provided to produce L-ribose of high specificity through an eco-friendly method. CONSTITUTION: A mannose-6-phosphate isomerase variant is prepared by converting 193th residue of the enzyme of sequence number 1, arginine(R) into alanine(A). The variant is also prepared by: converting 17th residue of the enzyme, tryptophan(W) into glutamine(Q); 90th residue, asparagine(N) into alanine(A); 129th residue leucine(L) into phenylalanine(F); and 193th residue, arginine(R) into alanine(A). A gene encoding the variant has a base of sequence number 2. A composition for producing ribose contains the variant.
Abstract translation: 目的：提供甘露糖-6-磷酸异构酶变体，通过环保方法生产高特异性的L-核糖。 构成：通过将序列号1的精氨酸（R）的第193残基转化为丙氨酸（A）来制备甘露糖-6-磷酸异构酶变体。 该变体还可通过以下方法制备：将第17残基的酶，色氨酸（W）转化成谷氨酰胺（Q）; 第90残基，天冬酰胺（N）转化成丙氨酸（A）; 第129残留亮氨酸（L）转化为苯丙氨酸（F）; 和第193残基，精氨酸（R）转化成丙氨酸（A）。 编码变体的基因具有序列号2的碱基。用于产生核糖的组合物含有该变体。
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Patent Agency Ranking
公开(公告)号：KR1020120140449A
公开(公告)日：2012-12-31
申请号：KR1020110060193
申请日：2011-06-21
Applicant: 건국대학교 산학협력단 , (주)인우코퍼레이션
CPC classification number: C12P7/6409 , C12N9/88 , C12Y402/01053
Abstract: PURPOSE: Producing high yields of 10-hidroxystearic acid by oleate hydratase is provided to produce high yields of 10-hydroxystearic acid as a source material used for industrial products, and usefully and environmentally friendly applied as a starting material of lactone. CONSTITUTION: A material for producing10-hydroxystearic acid comprises oleate hydratase with the amino acid sequence of sequence number 1 and organic servant. An enzyme is made from Lysinibacillus fusiformis. An organic solvent is ethanol. The Material for producing10-hydroxystearic acid comprises a gene capable of coding oleate hydratase with sequence number 2, and the organic solvent as an active ingredient. Producing 10-hydroxystearic acid comprises bioconversion in the organic solvent by using oleate hydratase to make hydroxyl fatty acid from unsaturated fatty acid, and oleate hydratase and a substrate. The substrate is oleic acid. A process is conducted from PH 6.0 to PH 7.0 and from 25°C to 30°C.
Abstract translation: 目的：提供油酸水合酶产生高产量的10-羟基硬脂酸，以产生高产量的10-羟基硬脂酸作为工业产品的原料，作为内酯起始原料有效和环保。 构成：用于生产10-羟基硬脂酸的材料包括具有序列1的氨基酸序列的油酸水合酶和有机仆人。 一种酶由梭杆菌（Lysinibacillus fusiformis）制成。 有机溶剂是乙醇。 用于生产10-羟基硬脂酸的材料包括能够编码具有序列号2的油酸水合酶的基因，并且有机溶剂作为活性成分。 生产10-羟基硬脂酸包括通过使用油酸水合酶从不饱和脂肪酸和油酸水合酶和底物制备羟基脂肪酸在有机溶剂中的生物转化。 底物是油酸。 一个工艺从PH6.0到PH7.0和25℃到30℃进行。
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127.发明公开
公开(公告)号：KR1020120131033A
公开(公告)日：2012-12-04
申请号：KR1020110049206
申请日：2011-05-24
Applicant: 건국대학교 산학협력단
CPC classification number: C12N9/90 , C12P19/02 , C12P19/24 , C12Y503/01008
Abstract: PURPOSE: A method for producing L-ribose by an enzyme reaction is provided to produce a large amount of L-ribose with high specificity. CONSTITUTION: A mutant mannose 6-phosphate isomerase of sequence number 2 is prepared by converting 17th amino acid residue of mannose 6-phosphate isomerase of sequence number 1, tryptophane(W) into glutamine(Q). A gene encoding the mannose 6-phosphate isomerase has a base of sequence number 3. A mutant mannose 6-phosphate isomerase of sequence number 4 is prepared by converting mannose 6-phosphate isomerase of sequence number 1, tryptopane(W) into glutamine(Q). A method for preparing ribose comprises a step of using L-ribulose as a substrate. The ribose is L-ribose.
Abstract translation: 目的：提供通过酶反应生产L-核糖的方法，以产生大量特异性的L-核糖。 构成：序列号2的突变型甘露糖-6-磷酸异构酶通过将序列号1的甘露糖-6-磷酸异构酶，色氨酸（W）的第17位氨基酸残基转化为谷氨酰胺（Q）来制备。 编码甘露糖6-磷酸异构酶的基因具有序列号3的碱基。序列号4的突变体甘露糖-6-磷酸异构酶通过将序列号1的甘露糖-6-磷酸异构酶，曲妥坦（W）转化为谷氨酰胺（Q ）。 制备核糖的方法包括使用L-核酮糖作为底物的步骤。 核糖是L-核糖。
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公开(公告)号：KR1020120069126A
公开(公告)日：2012-06-28
申请号：KR1020100130534
申请日：2010-12-20
Applicant: 건국대학교 산학협력단
CPC classification number: C12P19/02 , C12N9/90 , C12P19/24 , C12Y501/03008
Abstract: PURPOSE: A method for preparing mannose from glucose using N-acyl-D-glucosamine 2-apimerase is provided to efficiently obtain mannose and to lower blood glucose. CONSTITUTION: A method for preparing N-acyl-D-glucosamine 2-apimerase comprises: a step of preparing a recombinant expression vector, pET24a/Nacyl-D-glucosamine 2-epimerase; a step of culturing a microorganism transformed by the recombinant vector to induce expression of N-acyl-D-glucosamine 2-epimerase; and a step of isolating and purifying the recombinant protein. A composition for producing mannose contains N-acyl-D-glucosamine 2-apimerase as an active ingredient. N-acyl-D-glucosamine 2-apimerase is derived from Caldicellulosiruptor saccharolyticus strain.
Abstract translation: 目的：提供使用N-酰基-D-葡糖胺2-对二聚体酶从葡萄糖制备甘露糖的方法，以有效地获得甘露糖并降低血糖。 构成：制备N-酰基-D-葡糖胺2-对二聚体酶的方法包括：制备重组表达载体pET24a / Nacyl-D-glucosamine 2-差向异构酶的步骤; 培养由重组载体转化的微生物以诱导N-酰基-D-葡糖胺2-差向异构酶的表达的步骤; 以及分离和纯化重组蛋白的步骤。 用于制备甘露糖的组合物含有N-酰基-D-葡糖胺2-对二聚糖酶作为活性成分。 N-酰基-D-葡糖胺2-对二聚体酶衍生自卡氏纤维素酶解糖酵母菌株。
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公开(公告)号：KR1020110044689A
公开(公告)日：2011-04-29
申请号：KR1020100062366
申请日：2010-06-29
Applicant: 건국대학교 산학협력단
CPC classification number: C12N9/90 , C12P19/02 , C12P19/24 , C12Y503/01008
Abstract: PURPOSE: A novel mannose-6-phosphate isomerase and mutant thereof are provided to ensure high specificity and environmentally-friendly benefit. CONSTITUTION: A novel mannose-6-phosphate isomerase is denoted by sequence number 1. A gene encoding the mannose-6-phosphate isomerase has a base sequence of sequence number 2. A recombinant expression vector, pET 28a(+)/mannose 6-phosphate isomerase, contains the mannose-6-phosphate isomerase gene. A method for preparing mannose-6-phosphate isomerase comprises: a step of preparing an expression vector containing mannose-6-phosphate isomerase gene; a step of culturing transformed microorganism; and a step of isolating and purifying mannose-6-phosphate isomerase from the microorganism.
Abstract translation: 目的：提供新的甘露糖-6-磷酸异构酶及其突变体，以确保高特异性和环境友好的益处。 构成：新的甘露糖-6-磷酸异构酶由序列号1表示。编码甘露糖-6-磷酸异构酶的基因具有序列号2的碱基序列。重组表达载体pET28a（+）/甘露糖-6-磷酸异构酶 磷酸异构酶，含有甘露糖-6-磷酸异构酶基因。 制备甘露糖-6-磷酸异构酶的方法包括：制备含有甘露糖-6-磷酸异构酶基因的表达载体的步骤; 培养转化微生物的步骤; 以及从微生物中分离并纯化甘露糖-6-磷酸异构酶的步骤。
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130.发明公开푸사리움 모닐리포르메 바 써브글루티난스의 세포외 효소를 이용한 진세노사이드 알지원으로부터 진세노사이드 에프원의 생산방법 审中-实审人参皂甙Rg1通过镰刀菌变种的细胞外酶制备人参皂甙F1的方法 subglutinans
公开(公告)号：KR1020120121076A
公开(公告)日：2012-11-05
申请号：KR1020110038826
申请日：2011-04-26
Applicant: (주)아모레퍼시픽
CPC classification number: C12P33/20
Abstract: PURPOSE: A method for preparing rare ginsenoside F1 using extracellular enzyme of Fusarium moniliforme var. subglutinans is provided to suppress cancer cell proliferation and to enhance anticancer function. CONSTITUTION: A composition for preventing ginsenoside F1 contains extracellular enzyme derived from Fusarium moniliforme var. subglutinans. The optical temperature and pH of the extracellular enzyme is 30-70 Deg. C. and pH 4.0-7.0. A method for preparing ginsenoside F1 comprises: a step of culturing Fusarium moniliforme var. subglutinans; a step of preparing the extracellular enzyme from the cultured strain. [Reference numerals] (AA,DD) Absorbance(mAU); (BB,EE) Time(min); (CC) Ginsenoside Rg1; (FF) Ginsenoside F1
Abstract translation: 目的：使用镰刀菌变种的细胞外酶制备稀有人参皂苷F1的方法。 提供细胞外基质以抑制癌细胞增殖并增强抗癌功能。 构成：用于预防人参皂苷F1的组合物含有源自镰孢镰刀菌变种的细胞外酶。 subglutinans。 细胞外酶的光学温度和pH为30-70度。 C.和pH 4.0-7.0。 一种人参皂甙F1的制备方法，包括：培养镰孢镰孢变种 subglutinans; 从培养菌株制备细胞外酶的步骤。 （参考号）（AA，DD）吸光度（mAU）; （BB，EE）时间（分钟）; （CC）人参皂苷Rg1; （FF）人参皂苷F1
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