公开(公告)号：WO1997026008A1

公开(公告)日：1997-07-24

申请号：PCT/US1997001056

申请日：1997-01-21

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA ,  CLEARY, Paul, P.

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA

IPC: A61K39/09

CPC classification number: C12N9/52 ,  A61K39/00 ,  A61K39/092 ,  C12Y304/2111

Abstract: Novel vaccines for use against beta -hemolytic Streptococcus colonization or infection are disclosed. The vaccines contain an immunogenic amount of streptococcal C5a peptidase, or a fragment or mutant thereof. Also disclosed is a method of protecting a susceptible mammal against beta -hemolytic Streptococcus colonization or infection by administering such a vaccine.

Abstract translation: 公开了用于抗β-溶血性链球菌定居或感染的新型疫苗。 疫苗含有免疫原性量的链球菌C5a肽酶，或其片段或突变体。 还公开了通过施用这样的疫苗来保护易感染哺乳动物免受β-溶血性链球菌定植或感染的方法。

公开(公告)号：WO1997015675A1

公开(公告)日：1997-05-01

申请号：PCT/US1996016929

申请日：1996-10-23

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  WACKETT, Lawrence, P. ,  SADOWSKY, Michael, J. ,  DE SOUZA, Mervyn, L.

IPC: C12N15/55

CPC classification number: C12N9/14 ,  A62D3/02 ,  A62D2101/26 ,  B09C1/10 ,  C07K16/40

Abstract: An isolated and purified DNA molecule, and an isolated and purified protein, that are involved in the degradation of s-triazine compounds (e.g., atrazine) are provided. A method for the purification of this protein is also provided.

Abstract translation: 提供了分离和纯化的DNA分子，以及分离和纯化的蛋白质，其涉及s-三嗪化合物（例如莠去津）的降解。 还提供了用于纯化该蛋白质的方法。

公开(公告)号：WO1997004330A1

公开(公告)日：1997-02-06

申请号：PCT/US1996011891

申请日：1996-07-18

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA ,  HU, Xiaoping ,  HUU LE, Tuong ,  PARRISH, Todd, B. ,  ERHARD, Peter, W.

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA

IPC: G01R33/567

CPC classification number: G01R33/5676 ,  A61B5/7289 ,  G01R33/4806 ,  G01R33/56509 ,  G01R33/5673

Abstract: A magnetic resonance imaging (MRI) technique compensates for image-to-image fluctuation due to physiological motion to improve detection of cerebral and neuronal activity. Simultaneous monitoring of respiration and cardiac signals during imaging allows retrospective synchronization of imaging data with physiological activity. The technique extracts respiratory and cardiac motions directly from functional MRI data, but does not rely on the periodicity of the physiological signals; does not affect signal changes arising from neuronal activation; and is beneficial to images acquired at any speed. Direct extraction of physiological parameters can eliminate external monitoring. Respiratory cycles are preferably derived from the phase of the center of a navigator echo in rapid, low flip angle pulsed NMR imaging (FLASH) and from the phase of the center k-space point in echo planar imaging (EPI). Cardiac cycles are preferably determined from projections obtained from the navigator echo in FLASH and the center k-space lines in EPI.

Abstract translation: 磁共振成像（MRI）技术补偿由于生理运动引起的图像到图像波动，以改善脑和神经元活动的检测。 在成像期间同时监测呼吸和心脏信号可使成像数据与生理活动的回顾性同步。 该技术直接从功能性MRI数据提取呼吸和心脏运动，但不依赖于生理信号的周期; 不影响神经元激活引起的信号变化; 并且对以任何速度获取的图像是有益的。 直接提取生理参数可以消除外部监测。 呼吸周期优选地从快速，低翻转脉冲NMR成像（FLASH）中的导航回波中心的相位和从回波平面成像（EPI）中的中心k空间点的相位导出。 优选地，从FLASH中的导航回波和EPI中的中心k空间线获得的投影确定心脏周期。

公开(公告)号：WO1996040930A1

公开(公告)日：1996-12-19

申请号：PCT/US1996010252

申请日：1996-06-07

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA ,  SCHLIEVERT, Patrick, M. ,  ROGGIANI, Manuela ,  STOEHR, Jennifer ,  OHLENDORF, Douglas

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA

IPC: C12N15/31

CPC classification number: C07K14/315 ,  A61K38/00 ,  A61K39/00

Abstract: This invention is directed to mutant SPE-A toxins or fragments thereof, vaccine and pharmaceutical compositions, and methods of using the vaccine and pharmaceutical compositions. The preferred SPE-A toxin has at least one amino acid change and is substantially non-lethal compared with the wild type SPE-A toxin. The mutant SPE-A toxins can form vaccine compositions useful to protect animals against the biological activities of wild type SPE-A toxin.

Abstract translation: 本发明涉及突变体SPE-A毒素或其片段，疫苗和药物组合物，以及使用疫苗和药物组合物的方法。 与野生型SPE-A毒素相比，优选的SPE-A毒素具有至少一个氨基酸变化并且基本上是非致死的。 突变型SPE-A毒素可以形成用于保护动物免受野生型SPE-A毒素生物活性的疫苗组合物。

公开(公告)号：WO1996036360A1

公开(公告)日：1996-11-21

申请号：PCT/US1996006941

申请日：1996-05-15

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  KERSEY, John, H., Jr. ,  BEJCEK, Bruce, E. ,  WANG, Duo ,  UCKUN, Fatih, M.

IPC: A61K39/395

CPC classification number: C07K16/2803 ,  A61K38/00 ,  A61K47/6849 ,  A61K47/6867 ,  C07K16/3061

Abstract: Disclosed are polynucleotides encoding single chain variable region fragments of a monoclonal antibody to CD19 and methods for preparing the same. Also disclosed are single chain variable region polypeptides, methods for preparing the same, point modified polypeptides, and dimers derived therefrom. An additional aspect of the invention discloses immunoconjugates formed between a polypeptide of the invention and cytotoxic agents, as well as methods for their preparation, as well as use in the treatment of cancer.

Abstract translation: 公开了编码CD19单克隆抗体的单链可变区片段的多核苷酸及其制备方法。 还公开了单链可变区多肽，其制备方法，点修饰多肽和由其衍生的二聚体。 本发明的另一方面公开了本发明的多肽与细胞毒性剂之间形成的免疫偶联物，以及其制备方法，以及用于治疗癌症。

公开(公告)号：WO1996033149A1

公开(公告)日：1996-10-24

申请号：PCT/US1996004583

申请日：1996-04-03

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  BHARADWAJ, Sameer, S. ,  SCHMIDT, Lanny, D.

IPC: C07C05/327

CPC classification number: C07C5/48 ,  C07C2521/04 ,  C07C2521/06 ,  C07C2523/42 ,  C07C2523/46 ,  C07C2523/52 ,  C07C2523/755

Abstract: A process for the oxidative dehydrogenation of hydrocarbons is disclosed in which C2-C6 alkanes are contacted with an oxygen containing gas in a fluidized catalyst bed of platinum, rhodium, nickel or platinum-gold supported on alpha -alumina or zirconia. Ethane is dehydrogenated to ethylene and higher alkanes are dehydrogenated to ethylene, propylene and iso-butylene.

Abstract translation: 公开了一种烃的氧化脱氢方法，其中在负载在α-氧化铝或氧化锆上的铂，铑，镍或铂金的流化催化剂床中，C2-C6烷烃与含氧气体接触。 乙烷脱氢为乙烯，高级烷烃脱氢为乙烯，丙烯和异丁烯。

公开(公告)号：WO1996032847A1

公开(公告)日：1996-10-24

申请号：PCT/US1996005180

申请日：1996-04-16

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  HALVORSON, David, A. ,  CHAPLIN, Jonathan ,  HOLICKY, Scott, Richard ,  NOLL, Sally, L.

IPC: A22C21/00

CPC classification number: A22C21/0061 ,  A22C21/06

Abstract: A method of processing poultry comprises the opening of a poultry carcass (10) along at least one lateral side (16) from the thoracic inlet to about the midline of the abdomen, to permit separating a breast portion (20) of the poultry carcass (10) from the spine (24, 41) by hinging along an opposite lateral side from the initial severing line. This opens the carcass for evaluation of bacterial contamination of internal organs (38). The carcass (10) is opened along a line where few bones need to be severed, and the internal organs (38) are not broken or torn, which is a major cause of contamination in conventional poultry processing methods. The longitudinal severing line extends from the neck to the pubis and when the breast portion (20), including one wing (14) is moved, air sac membranes and fat layers will contain the internal organs (38) and form separation planes to separate the breast portion (20) from the liver (42), spleen, heart (44) and intestines. The processing can be mechanized or done by hand.

Abstract translation: 一种处理家禽的方法包括：沿着从胸部入口到大约中腹线的至少一个侧面（16）打开家禽屠体（10），以允许分离家禽屠体的乳房部分（20） 10）从脊部（24,41）通过沿着与初始切断线相反的侧面的铰接。 这打开了用于评估内脏细菌污染的胴体（38）。 屠体（10）沿着需要切断骨头的线路开放，并且内部器官（38）不破裂或撕裂，这是常规家禽加工方法中的主要污染原因。 纵向切断线从颈部延伸到耻骨，并且当包括一个翼（14）的乳房部分（20）被移动时，气囊膜和脂肪层将包含内部器官（38）并形成分离平面以分离 来自肝脏（42），脾脏，心脏（44）和肠的乳房部分（20）。 加工可以手工机械化或完成。

公开(公告)号：WO1996031609A2

公开(公告)日：1996-10-10

申请号：PCT/US1996004625

申请日：1996-04-04

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA ,  GENGENBACH, Burle, G. ,  SOMERS, David, A. ,  WYSE, Donald, L. ,  GRONWALD, John, W. ,  EGLI, Margaret, A. ,  LUTZ, Sheila, M.

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA

IPC: C12N15/52

CPC classification number: C12N9/93 ,  C12N15/8247 ,  C12N15/8274

Abstract: The present invention provides the complete cDNA sequence of maize acetyl CoA carboxylase and methods for conferring herbicide tolerance and/or altering the oil content of plants by introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. The method of imparting herbicide tolerance to a plant includes the steps of introducing an expression cassette encoding a plant acetyl CoA carboxylase or an antisense DNA sequence complementary to the sequence for a plant acetyl CoA carboxylase gene operably linked to a promoter functional in plant cells, into the cells of a plant tissue and expressing the plant acetyl CoA carboxylase gene in an amount effective to render the acetyl CoA carboxylase and/or plant cell tolerant to the herbicides. The method of altering the oil content in a plant includes the steps of introducing an expression cassette into plant cells and expressing the acetyl CoA carboxylase gene in an amount effective to alter the oil content of the cells. The expression cassette can also be introduced into other host cells to increase yield of a plant acetyl CoA carboxylase so that crystallized enzyme can be used to screen and identify other herbicides that bind to and inhibit the enzyme.

Abstract translation: 本发明提供玉米乙酰CoA羧化酶的完整cDNA序列，以及通过在植物细胞中引入和表达植物乙酰CoA羧化酶基因来赋予除草剂耐受性和/或改变植物油含量的方法。 对植物赋予除草剂耐受性的方法包括以下步骤：将编码植物乙酰辅酶A羧化酶的表达盒或与植物乙酰辅酶A羧化酶基因互补的反义DNA序列引入到与植物细胞中有功能的启动子连接的植物乙酰辅酶A羧化酶基因， 植物组织的细胞并以有效使得乙酰辅酶A羧化酶和/或植物细胞对除草剂具有耐受性的量表达植物乙酰辅酶A羧化酶基因。 改变植物油含量的方法包括将表达盒引入植物细胞并以有效改变细胞油含量的量表达乙酰CoA羧化酶基因的步骤。 也可以将表达盒引入其他宿主细胞中以增加植物乙酰CoA羧化酶的产量，使得结晶的酶可用于筛选和鉴定结合并抑制酶的其它除草剂。

公开(公告)号：WO1996021467A1

公开(公告)日：1996-07-18

申请号：PCT/US1996000104

申请日：1996-01-11

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  UCKUN, Faith, M. ,  ANDERSON, Peter, M.

IPC: A61K47/48

CPC classification number: A61K47/6825 ,  Y10S530/866

Abstract: Cytotoxic biotherapeutic agents effective for treating certain types of cancer in humans are provided which comprise the TP-3 murine monoclonal antibody chemically conjugated to pokeweed antiviral protein (PAP). The invention further provides a method which utilizes the disclosed cytotoxic biotherapeutic agents to systemically treat cancer patients. With slight modifications the method of the present invention should be generally applicable to preparation and use of other cytotoxic biotherapeutic agents using chemical or recombinant derivatives of the TP-3 or TP-1 antibodies or PAP toxin. The invention is applicable to cancer patients who express the p80 antigen recognized by the TP-1/TP-3 antibodies either on the surface of their tumor cells or on the tumor blood vessels.

Abstract translation: 提供了有效治疗人类某些类型癌症的细胞毒性生物治疗剂，其包含与pokeweed抗病毒蛋白（PAP）化学偶联的TP-3鼠单克隆抗体。 本发明进一步提供利用所公开的细胞毒性生物治疗剂系统地治疗癌症患者的方法。 通过轻微修改，本发明的方法应通常适用于使用TP-3或TP-1抗体或PAP毒素的化学或重组衍生物制备和使用其它细胞毒性生物治疗剂。 本发明适用于在肿瘤细胞表面或肿瘤血管上表达由TP-1 / TP-3抗体识别的p80抗原的癌症患者。

公开(公告)号：WO1996004010A1

公开(公告)日：1996-02-15

申请号：PCT/US1995009927

申请日：1995-08-04

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  MURTAUGH, Michael, P. ,  ELAM, Margaret, R. ,  KAKACH, Laura, T.

IPC: A61K39/12

CPC classification number: C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N2770/10022

Abstract: A nucleotide sequence is provided for the VR-2332 virus, which is capable of causing Porcine Reproductive and Respiratory Syndrome. The nucleotide sequence includes protein coding regions that are inserted into recombinant vectors for the host expression of viral proteins according to a variety of vaccination techniques. Diagnostic assays utilize fragmentary sequences or oligonucleotides to selectively identify the VR-2332 nucleic acids by hybridization or PCR amplification reactions that distinguish VR-2332 nucleotide sequences from other PRRS-causative viruses which are immunologically distinct from VR-2332.

Abstract translation: 为VR-2332病毒提供了能够引起猪繁殖与呼吸综合征的核苷酸序列。 核苷酸序列包括根据各种疫苗接种技术插入病毒蛋白宿主表达重组载体的蛋白质编码区。 诊断测定利用片段序列或寡核苷酸通过杂交或PCR扩增反应选择性鉴定VR-2332核酸，区分VR-2332核苷酸序列与其他与VR-2332免疫学区别的PRRS致病病毒。