公开(公告)号：EP0979311A1

公开(公告)日：2000-02-16

申请号：EP98920930.9

申请日：1998-04-30

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  STEER, Clifford, J. ,  KREN, Betsy, T. ,  BANDYOPADHYAY, Paramita, T.

IPC: C12Q1/68 ,  A61K48/00 ,  C07H21/04

CPC classification number: C12N15/102 ,  A61K9/1075 ,  A61K9/1272

Abstract: The present invention concerns compositions and methods for the introduction of specific genetic changes in endogenous genes of the cells of an animal. The genetic changes are effected by oligonucleotides or oligonucleotide derivatives and analogs, which are generally less than about 100 nucleotides in length. The invention provides for macromolecular carriers, optionally incorporating ligands for clathrin coated pit receptors. In one embodiment the ligand is a lactose or galactose and the genetic changes are made in hepatocytes. By means of the invention up to 40 % of the copiies of a target gene have been changed in vitro. Repair of mutant genes having a Crigler-Najjar like phenotype and Hemophilia B phenotype were observed.

公开(公告)号：WO1998006824A1

公开(公告)日：1998-02-19

申请号：PCT/US1997014384

申请日：1997-08-13

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  FOSTER, Douglas, N. ,  FOSTER, Linda, K.

IPC: C12N05/06

CPC classification number: C12N5/0652 ,  C12N7/00 ,  C12N2510/02 ,  C12N2510/04 ,  C12N2710/16651 ,  C12N2720/12051 ,  C12Q1/02

Abstract: This invention relates to the production and use of immortalized cell lines from primary chicken embryonic fibroblasts. The cells are useful as substrates for virus propagation, recombinant protein expression and recombinant virus production.

Abstract translation: 本发明涉及来自原代鸡胚胎成纤维细胞的永生化细胞系的生产和使用。 细胞可用作病毒繁殖，重组蛋白表达和重组病毒生产的底物。

公开(公告)号：WO1997046892A2

公开(公告)日：1997-12-11

申请号：PCT/US1997009658

申请日：1997-06-05

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  DAHLBERG, E., Dan ,  MORAN, Timothy, J.

IPC: G01R33/09

CPC classification number: B82Y25/00 ,  B82Y10/00 ,  G01C17/30 ,  G01R33/04 ,  G01R33/09 ,  G01R33/096 ,  G11B5/012 ,  G11B5/3903 ,  G11B5/3932 ,  G11B5/399 ,  G11B5/465 ,  G11B2005/0016 ,  G11B2005/3996 ,  H01F10/32 ,  H01F10/324 ,  H01L43/10

Abstract: The present invention provides a method and apparatus for utilizing magnetoresistance devices for the measurement of weak magnetic fields. An oscillating excitation magnetic field is applied to a magnetoresistive (MR) sensing element such that the MR element is driven into one or both of two antiparallel saturation states. The amplitude of the excitation field is large enough to reverse the magnetization of the soft layer during each cycle. In one embodiment, the MR element is provided with a current, and a voltage proportional to the resistance is measured. Components of the voltage signal at multiples of the excitation frequency are then proportional to the environmental magnetic field. In one embodiment, an MR element having a resistance-versus-field transfer function that is symmetric (e.g., an anisotropic MR element) is used; while in another embodiment, an MR element having a resistance-versus-field transfer function that is asymmetric (e.g., a spin-valve MR element) is used. Various apparatus and methods for measuring the amount of time spent in one or both saturated states versus the unsaturated or transition states are described. In one embodiment, the magentic excitation field is generated using a current strip deposited onto the top of the other device layers, so that the entire device can be produced on a single chip. In one embodiment, a "flexible" magnetoresistive structure includes a "flexible" ferromagnetic layer having a hard-magnetization-portion layer, and a soft-magnetization-portion layer, thus providing a smooth magnetic transition when this bilayer switches. One embodiment includes s supporting data-read head structure that positions the flexible magnetoresistive (MR) sensing element to sense a magnetic field in a data storage device such as a magnetic-disk drive.

Abstract translation: 本发明提供一种用于利用磁阻器件测量弱磁场的方法和装置。 将振荡激励磁场施加到磁阻（MR）感测元件，使得MR元件被驱动成两个反平行饱和状态中的一个或两个。 励磁场的振幅足够大以在每个周期期间逆转软层的磁化。 在一个实施例中，向MR元件提供电流，并且测量与电阻成比例的电压。 激励频率倍数的电压信号的分量然后与环境磁场成比例。 在一个实施例中，使用具有对称的电阻对场传递函数（例如，各向异性MR元件）的MR元件; 而在另一个实施例中，使用具有不对称的电阻对场传递函数（例如，自旋阀MR元件）的MR元件。 描述了用于测量在一个或两个饱和状态下消耗的时间量与不饱和或过渡态的各种装置和方法。 在一个实施例中，使用沉积在其它器件层的顶部上的电流条来产生磁场激励场，使得可以在单个芯片上产生整个器件。 在一个实施例中，“柔性”磁阻结构包括具有硬磁化部分层和软磁化部分层的“柔性”铁磁层，从而在该双层开关时提供平滑的磁转变。 一个实施例包括支持数据读取头结构，其定位柔性磁阻（MR）感测元件以感测诸如磁盘驱动器的数据存储设备中的磁场。

公开(公告)号：WO1997043419A2

公开(公告)日：1997-11-20

申请号：PCT/US1997008765

申请日：1997-05-16

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA ,  OLSZEWSKI, Neil, E. ,  JACOBSEN, Steven, E.

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA

IPC: C12N15/29

CPC classification number: C07K14/415 ,  C12N15/8297

Abstract: This invention relates to the identification of a gene involved in the gibberellin signal transduction pathway. Mutations to this gene mimic the effect of gibberellin treatment and transgenic plants expressing the gene correct a spindly phenotype. Methods are disclosed for isolating and using the gene from a variety of plants.

Abstract translation: 本发明涉及鉴定参与赤霉素信号转导途径的基因。 该基因的突变模拟赤霉素处理的效果和表达该基因的转基因植物校正一种直立表型。 公开了用于分离和使用来自多种植物的基因的方法。

公开(公告)号：WO1997029062A1

公开(公告)日：1997-08-14

申请号：PCT/US1997001848

申请日：1997-02-07

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  GOETSCH, Duane, A. ,  SCHMIDT, Lanny, D.

IPC: C07B33/00

CPC classification number: C07C45/33 ,  C07B41/00 ,  C07C5/48 ,  C07C27/14 ,  C07C2523/42 ,  C07C2523/46 ,  C07C11/02

Abstract: A process for catalytic partial oxidation of a hydrocarbon is pro vided. The process includes: providing a feed gas mixture comprising an oxygen-containing gas and a hydrocarbon gas comprising one or more normal (C2-C4)alkanes; providing a catalyst structure having a transparency of at least about 40 %; and passing the feed gas mixture through the catalyst structure at a rate such that the superficial contact time of the feed gas mixture with the catalyst structure is no greater than about 1000 microseconds to produce an exit gas mixture comprising partial oxidation products.

Abstract translation: 提供了一种烃的催化部分氧化方法。 该方法包括：提供包含含氧气体和包含一种或多种正常（C 2 -C 4）烷烃的烃气体的进料气体混合物; 提供具有至少约40％的透明度的催化剂结构; 并且使进料气体混合物通过催化剂结构，使得原料气体混合物与催化剂结构的表面接触时间不大于约1000微秒，以产生包含部分氧化产物的出口气体混合物。

公开(公告)号：WO1997022711A1

公开(公告)日：1997-06-26

申请号：PCT/US1996020119

申请日：1996-12-18

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA ,  SHERMAN, David, H. ,  WILLIAMS, Mark, D. ,  XUE, Yongquan

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA

IPC: C12P07/62

CPC classification number: C12N9/1025 ,  C12N15/52 ,  C12P7/42 ,  C12P7/625

Abstract: A novel pathway for the synthesis of polyhydroxyalkanoates is provided. A method of synthesizing a recombinant polyhydroxyalkanoate monomer synthase is also provided. These recombinant polyhydroxyalkanoate synthases are derived from multifunctional fatty acid synthases or polyketide synthases and generate hydroxyacyl acids capable of polymerization by a polyhydroxyalkanoate synthase.

Abstract translation: 提供了一种用于合成聚羟基链烷酸酯的新途径。 还提供了合成重组聚羟基链烷酸酯单体合酶的方法。 这些重组聚羟基链烷酸酯合成酶衍生自多官能脂肪酸合成酶或聚酮化合物合成酶，并产生能够通过聚羟基链烷酸酯合成酶聚合的羟基酸。

公开(公告)号：WO1997018003A1

公开(公告)日：1997-05-22

申请号：PCT/US1996018042

申请日：1996-11-12

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  WARWICK, Warren, J. ,  HANSEN, Leland, G.

IPC: A61M16/00

CPC classification number: A61M15/00 ,  A61B5/087 ,  A61M16/0006 ,  A61M16/0051 ,  A61M16/0063 ,  A61M16/022 ,  A61M16/08 ,  A61M16/0833 ,  A61M16/0858 ,  A61M16/0866 ,  A61M2016/0021 ,  A61M2016/0039

Abstract: An air flow system (10) for detecting target flow rates as a function of an opening (32, 42) in a flow restrictor (18, 20). A negative pressure sensor (50, 52) is triggered in response to a negative pressure condition in a chamber (23, 25) in the flow restrictor (18, 20). Target flow rates are preselected by using a flow restrictor (18, 20) having a particular size restrictor. Triggering of the negative pressure sensor (50, 52) may be used for controlling various medical treatments. A signal generated when a target flow rate is reached may be used for administering aerosols during inhalation, activation of HFCC therapy during exhalation, or to control various other medical treatments. The air flow system (10) allows for selection of a narrow flow rate range for administering aerosols during inhalation to target particular areas in the respiratory tree as major deposition sites for the aerosols.

Abstract translation: 一种气流系统（10），用于根据限流器（18,20）中的开口（32,42）检测目标流量。 负压传感器（50,52）响应于限流器（18,20）中的腔室（23,25）中的负压条件而被触发。 通过使用具有特定尺寸限制器的限流器（18,20）来预先选择目标流量。 负压传感器（50,52）的触发可以用于控制各种医疗处理。 当达到目标流速时产生的信号可用于在吸入期间施用气溶胶，在呼气期间激活HFCC治疗或控制各种其它医疗治疗。 气流系统（10）允许选择窄的流速范围，用于在吸入期间施用气溶胶以将呼吸树中的特定区域作为气溶胶的主要沉积位置。

公开(公告)号：WO1997000696A1

公开(公告)日：1997-01-09

申请号：PCT/US1996010595

申请日：1996-06-19

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  JOO, Han, Soo

IPC: A61K39/155

CPC classification number: A61K39/155 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/543 ,  A61K2039/552 ,  C12N2770/10034

Abstract: Live or modified live PRRS vaccines for administration to swine are provided which are of low virulence and confer effective immunity against PRRS. The preferred vaccines include virus isolates having average plaque diameters of less than about 2 mm and low pathogenicity. A preferred vaccine includes a small plaque diameter strain, ATCC Accession No. VR2509. The vaccines of the invention may be administered to breeding females or gilts and to weaned piglets, and is effective to immunize the swine against both the respiratory and reproductive forms of the disease.

Abstract translation: 提供了用于猪施用的活的或修饰的活PRRS疫苗，其具有低毒力并赋予对PRRS的有效免疫力。 优选的疫苗包括具有小于约2mm的平均斑块直径和低致病性的病毒分离物。 优选的疫苗包括小斑块直径菌株ATCC保藏号VR2509。 本发明的疫苗可以施用于育种雌性或后备母猪和断奶仔猪，并且有效地免疫猪抵抗该疾病的呼吸和生殖形式。

公开(公告)号：WO1997000273A1

公开(公告)日：1997-01-03

申请号：PCT/US1996010291

申请日：1996-06-14

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA ,  KEMPE, Maria ,  BARANY, George

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA

IPC: C08F20/28

CPC classification number: C07K1/042 ,  C08F20/28 ,  C08F22/105 ,  C08F290/062

Abstract: A polymer useful as a solid support for a variety of applications is provided. The polymer is prepared from a composition comprising at least one type of olefinic monomer and a multifunctional (meth)acrylate cross-linker of formula (I), wherein (a) R , R , and R are each independently hydrogen or a methyl group; (b) R is hydrogen or an organic group or substituent that can interact in the polymerization and/or cross-linking process or is nonreactive under the conditions of the polymerization and/or cross-linking process; (c) R , R , and R are each independently -CH2-CH2-, -CH2-CH2-CH2-, -CH2-CH(CH3)-, or -CH(CH3)-CH2-; (d) each of 1, m, and n is no greater than about 100 with the proviso that at least one of 1, m, or n is at least 1.

Abstract translation: 提供了可用作各种应用的固体支持物的聚合物。 聚合物由包含至少一种类型的烯属单体和式（I）的多官能（甲基）丙烯酸酯交联剂）的组合物制备，其中（a）R 1，R 2和R 3， 各自独立地为氢或甲基; （b）R 4是氢或可在聚合和/或交联过程中相互作用的有机基团或取代基，或在聚合和/或交联过程的条件下是非反应性的; （c）R 7，R 8和R 9各自独立地为-CH 2 -CH 2 - ， - CH 2 -CH 2 -CH 2 - ， - CH 2 -CH（CH 3） - 或-CH（CH 3） -CH 2 - （d）1，m和n中的每一个不大于约100，条件是1，m或n中的至少一个至少为1。

公开(公告)号：WO1996015111A1

公开(公告)日：1996-05-23

申请号：PCT/US1995014896

申请日：1995-11-13

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  HOYE, Thomas, R.

IPC: C07D217/24

CPC classification number: C07C43/23 ,  C07C43/225 ,  C07C43/315 ,  C07C309/65 ,  C07D217/02 ,  C07D217/04 ,  C07F5/025 ,  C07F7/1804 ,  Y02P20/55

Abstract: A method of preparing a korupensamine or an analog thereof comprising: (a) reacting a compound of formula (III), wherein each of R and R is CH3 or H, X is I, Y is (C1-C4)alkyl, benzyl or CHO, and each of R and R is (C1-C4)alkyl, benzyl, (C2-C5)acyl or an acid-labile hydroxy protecting group; with a compound of formula (IV), wherein R is benzyl, (C2-C5)acyl or an acid-labile hydroxy protecting group, R is B(OH)2, and R is (C1-C4)alkyl; in the presence of a Pd(0) catalyst and an inorganic base in an organic solvent, to yield a compound of formula (V), wherein Y, R , R , R , R , R and R are as defined above for compounds of formula (III) and (IV). Additionally the intermediates of formula (III), wherein X is Br, Cl or I, Y is H, (C1-C4)alkyl, benzyl, or CHO, each of R and R 3 is a protecting group selected from the group consisting of (C1-C4)alkyl, benzyl, (C2-C5)acyl and an acid-labile hydroxy protecting group; and R is H or (C2-C5)acyl; or wherein X is Br, Cl or I, Y is H, (C1-C4)alkyl, benzyl, or CHO, each of R and R is H or CH5, R is H or (C2-C5)acyl and R is a protecting group selected from the group consisting of (C1-C4)alkyl, benzyl, (C2-C5)acyl and an acid-labile hydroxy protecting group; and the intermediates of formula (IV), wherein R is Cl, Br, I, B(OH)2, an anhydride or ester of B(OH)2, or OSO2R , wherein R is (C1-C4)perfluoroalkyl, and each of R and R is H, (C1-C4)alkyl, benzyl, (C2-C5)acyl or an acid-labile hydroxy protecting group.

Abstract translation: 一种制备高甘露聚糖或其类似物的方法，包括：（a）使式（III）化合物与其中R 1和R 2各自为CH 3或H的化合物反应，X为I，Y为（C1- C4）烷基，苄基或CHO，R 3和R 4各自为（C 1 -C 4）烷基，苄基，（C 2 -C 5）酰基或酸不稳定羟基保护基; 与式（IV）化合物反应，其中R 5为苄基，（C 2 -C 5）酰基或酸不稳定羟基保护基，R 6为B（OH）2，R 7为（ C1-C4）烷基; 在Pd（0）催化剂和无机碱存在下，在有机溶剂中，得到式（Ⅴ）化合物，其中Y，R 1，R 2，R 3，R 4 >，R 5和R 7如上文对式（III）和（IV）化合物所定义。 另外，式（III）的中间体，其中X是Br，Cl或I，Y是H，（C1-C4）烷基，苄基或CHO，R 1和R 2各自为H或CH 3 <，R 3>是选自（C 1 -C 4）烷基，苄基，（C 2 -C 5）酰基和酸不稳定羟基保护基的保护基; 和R 4是H或（C 2 -C 5）酰基; 或其中X是Br，Cl或I，Y是H，（C1-C4）烷基，苄基或CHO，R 1和R 2各自是H或CH 5，R 3是H或（ C 2 -C 5）酰基和R 4是选自（C 1 -C 4）烷基，苄基，（C 2 -C 5）酰基和酸不稳定羟基保护基的保护基; 和式（Ⅳ）的中间体，其中R 6是Cl，Br，I，B（OH）2，B（OH）2的酸酐或酯或OSO 2 R 9，其中R 9是 （C 1 -C 4）全氟烷基，R 5和R 7各自为H，（C 1 -C 4）烷基，苄基，（C 2 -C 5）酰基或酸不稳定羟基保护基。