公开(公告)号：WO1991011462A2

公开(公告)日：1991-08-08

申请号：PCT/US1991000631

申请日：1991-01-30

Applicant: LA JOLLA CANCER RESEARCH FOUNDATION ,  WASHINGTON UNIVERSITY

Inventor： LA JOLLA CANCER RESEARCH FOUNDATION ,  WASHINGTON UNIVERSITY ,  ENGVALL, Eva ,  LEIVO, Ilmo ,  SANES, Joshua, R.

IPC: C07K13/00

CPC classification number: G01N33/68 ,  A61K38/00 ,  C07K14/78 ,  C07K16/00 ,  C12Q1/6876 ,  C12Q2600/158

Abstract: This invention provides a purified protein, having an apparent molecular weight of about 800 kDa, designated merosin. Also provided is a substantially pure heterotrimeric laminin variant comprising the structure M-X-B2, wherein M is the M polypeptide of merosin; X is selected from the group consisting of the B1 chain of laminin and S-laminin; and B2 is the B2 chain of laminin. Also provided is an isolated nucleic acid sequence which encodes merosin. The invention further provides antibodies, vectors, and the expression of recombinant proteins by use of a host vector system. The invention also provides the use of merosin to promote neurite growth.

Abstract translation: 本发明提供了具有约800kDa的表观分子量的纯化蛋白质，称为merosin。 还提供了包含结构M-X-B2的基本上纯的异三聚体层粘连蛋白变体，其中M是梅洛辛的M多肽; X选自层粘连蛋白和S-层粘连蛋白的B1链; B2是层粘连蛋白的B2链。 还提供了编码merosin的分离的核酸序列。 本发明还通过使用宿主载体系统进一步提供抗体，载体和重组蛋白的表达。 本发明还提供了merosin促进神经突生长的用途。

公开(公告)号：WO1990002484A1

公开(公告)日：1990-03-22

申请号：PCT/US1989003799

申请日：1989-09-05

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  CURTISS, Roy, III ,  CARDINEAU, Guy, A.

IPC: A01H05/00

CPC classification number: C12N9/1051 ,  A61K39/00 ,  C07K14/245 ,  C07K14/315 ,  C07K2319/00 ,  C12N9/16 ,  C12N9/2454 ,  C12N15/8258 ,  C12Y301/03001 ,  C12Y302/01011 ,  Y02A50/403 ,  Y02A50/476 ,  Y02A50/478 ,  Y02A50/48

Abstract: The invention is directed to transgenic plants expressing colonization and/or virulence antigens specified by genes from pathogenic microorganisms. It is also directed to the use of such transgenic plants for oral immunization of humans and other animals to elicit a secretory immune response which inhibits colonization of or invasion by such pathogenic microorganisms through a mucosal surface of humans or other animals.

Abstract translation: 本发明涉及表达由病原微生物的基因指定的定植和/或毒力抗原的转基因植物。 还涉及这样的转基因植物用于人和其他动物的口服免疫以引发分泌免疫应答，其通过人或其他动物的粘膜表面抑制这种致病微生物的定植或侵袭。

公开(公告)号：WO1989012680A1

公开(公告)日：1989-12-28

申请号：PCT/US1989002656

申请日：1989-06-16

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  SOBEL, Burton, E.

IPC: C12N09/48

CPC classification number: C12N9/6459 ,  A61K38/00 ,  A61K51/08 ,  A61K2123/00 ,  C12Y304/21069 ,  G01N33/86

Abstract: Modified forms of tPA which lack clot-dissolving activity but retain their ability to bind fibrin are useful as carriers of scintographic labels to permit the detection and location of blood clots in the circulation system of animals. These tracers can be injected intravenously, and then detected by scintographic imaging.

Abstract translation: 缺乏凝块溶解活性但保留其结合纤维蛋白的能力的tPA的修饰形式可用作扫描标记的载体，以允许在动物循环系统中检测和定位血块。 这些示踪剂可以静脉注射，然后通过扫描成像检测。

公开(公告)号：WO1987006462A1

公开(公告)日：1987-11-05

申请号：PCT/US1987001021

申请日：1987-05-01

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  COVEY, Douglas, F. ,  AUCHUS, Richard, J.

IPC: A61K31/045

CPC classification number: C07C62/38 ,  C07C39/17 ,  C07C39/23 ,  C07C2603/26

Abstract: Compounds of formula (I) and the pharmaceutically acceptable esters thereof, wherein rings A and B are selected from the group consisting of formulae (a), (b), (c), (d), wherein x represents an aromatic ring, or the presence or absence of 0, 1, or 2 conjugated or unconjugated pi bond(s), and wherein x' represents the presence or absence of 0, 1, or 2 conjugated or unconjugated pi bond(s), and wherein a non-aromatic pi bond, if present, may optionally be converted to an epoxide; and wherein the dotted line ------ represents the presence or absence of a pi bond, and wherein: R1 is H, acyl(1-6), or alkyl(1-6); R2 is H, alkyl(1-6), 2-propynyl, or allenyl; R3 and R4 is each independently H or OR5, wherein R5 is H, acyl(1-6), or alkyl(1-6); and wherein R1 and R2 is each independently H, alkyl(1-6) or is -C=CR3, -CH=CHR3, or -CH=C=CHR3, wherein R3 is selected from the group consisting of H, halo, CF3, alkyl(1-6), acyloxy, carboxylate, carboalkoxylate, alkoxy, or alkylthio; and wherein when at least one of R1 and R2 is H, the compound of formula (I) may also be in the oxidized oxo form and when both R1 and R2 are H, the compound of formula (I) may also be in the oxidized carboxylic acid form or the alkyl(1-6) ester thereof are useful in regulating steroid metabolism.

公开(公告)号：WO1987003094A1

公开(公告)日：1987-05-21

申请号：PCT/US1986002455

申请日：1986-11-14

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  LADENSON, Jack, H. ,  VAIDYA, Hemant, C. ,  DIETZLER, David, N. ,  MAYNARD, Ann, Yvonne

IPC: G01N33/53

CPC classification number: G01N33/573

Abstract: An improved method of determining CK-MB isoenzyme in a biological fluid which comprises subjecting a sample of said fluid to an assay system which includes incubating with monoclonal antibody specific to CK-MB isoenzyme, but not reactive with CK-MM or CK-BB. Methods for preparing antibodies with these characteristics and cell lines producing them are also disclosed.

公开(公告)号：WO1987001955A1

公开(公告)日：1987-04-09

申请号：PCT/US1986002038

申请日：1986-09-26

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  CARLE, Georges, F. ,  OLSON, Maynard, V.

IPC: B01D13/02

CPC classification number: B01D57/02 ,  G01N27/44773

Abstract: A method and apparatus for gel electrophoresis which employs periodic inversion of the electric field essentially in one dimension, denoted as field-inversion gel electrophoresis (FIGE), results in net migration by using a longer time or higher voltage in one direction than in the opposite direction. FIGE permits separation of DNA or protein mixtures in size ranges not accessible to ordinary electrophoresis.

Abstract translation: 凝胶电泳的方法和装置，其基本上在一维上采用电场周期反演，表示为场反转凝胶电泳（FIGE），通过在一个方向上使用比在相反方向上更长的时间或更高的电压来实现净迁移 方向。 FIGE允许在普通电泳不可接近的尺寸范围内分离DNA或蛋白质混合物。