公开(公告)号：US4628026A

公开(公告)日：1986-12-09

申请号：US552090

申请日：1983-11-15

Applicant: Dietlind Gardell ,  Gail Rock

Inventor： Dietlind Gardell ,  Gail Rock

IPC: G01N21/25 ,  G01N21/64 ,  G01N33/53 ,  G01N33/554

CPC classification number: G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2201/0446 ,  G01N2201/0484 ,  Y10S435/963 ,  Y10S435/966 ,  Y10S435/973 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/809 ,  Y10S436/821

Abstract: An automated system for rapid sequential photometric analysis of a collection of double fluorochrome stained lymphocyte specimens, useful for antibody screening or lymphocytotoxicity analysis. The specimens are sequentially alternately irradiated with light of two distinguishable wavelengths, producing fluorescence at two distinguishable wavelengths. The fluorescent emission light intensity for each irradiation of each specimen is measured using a photometer and computer. The computer controls the synchronization of the irradiation through alternately selected condenser sets with the sequential movement of specimens into the optical path of the irradiating and detected light, and calculates the quotient of the light intensities emitted from each specimen at the two selected fluorescent light wavelengths. These quotients are compared against a control ratio (for lymphocytotoxicity analysis) to classify the specimen. Also described is a method of preparing specimens for such analysis, which requires that a complement be added to the first staining solution after the latter is applied to the specimens, then this combination agitated, and then the second staining solution added and the specimen incubated.

Abstract translation: 一种用于快速顺序光度分析双重荧光染色淋巴细胞标本的自动化系统，可用于抗体筛选或淋巴细胞毒性分析。 用两个可辨别的波长的光顺序地交替地照射样品，在两个可区别的波长处产生荧光。 使用光度计和计算机测量每个样品的每次照射的荧光发射光强度。 计算机通过交替选择的聚光器组来控制照射的同步，同时将样品顺序移动到照射和检测光的光路中，并计算在两个选定的荧光波长处从每个样品发射的光强度的商。 将这些商与对照比（用于淋巴细胞毒性分析）进行比较以对样品进行分类。 还描述了制备用于这种分析的样品的方法，其要求在将样品应用于样品后将补体添加到第一染色溶液中，然后将该组合搅拌，然后加入第二染色溶液并将样品孵育。

公开(公告)号：WO2011008299A2

公开(公告)日：2011-01-20

申请号：PCT/US2010/002012

申请日：2010-07-16

Applicant: GOLD STANDARD DIAGNOSTICS CORP. ,  VAN PRAET, Peter

Inventor： VAN PRAET, Peter

IPC: G01N9/24 ,  G01N21/17 ,  G01N35/00

CPC classification number: G01N21/00 ,  G01N21/253 ,  G01N2201/0446 ,  G01N2201/0453 ,  G01N2201/0627 ,  G01N2201/10

Abstract: Light emitting diodes (LEDs) are mounted in an array to an upper structure overlying a lower structure with a plurality of light detectors thereon. Each LED is configured to overlie a separate detector. Each LED emits light at a frequency relevant for measuring optical density of a specimen. LEDs having different frequencies are included within the LED array. A corresponding array of detectors is also provided, mounted to the lower structure. Spacing between adjacent LEDs and between adjacent detectors match a spacing between wells in a microtiter plate. Spacing between the lower structure and the upper structure supporting the LEDs is sufficient for the microtiterplate to fit between. Circuitry sequentially fires individual LEDs and gathers optical density data through the detectors for specimens in the wells of the microtiter plate. The structures are then moved to a next adjacent well position on the microtiter plate and the process repeated.

Abstract translation: 发光二极管（LED）以阵列安装到上部结构上，其上覆盖具有多个光检测器的下部结构。 每个LED被配置为覆盖单独的检测器。 每个LED以与测量样本光密度相关的频率发光。 具有不同频率的LED被包括在LED阵列内。 还提供了相应的检测器阵列，安装在下部结构上。 相邻LED之间以及相邻检测器之间的间距与微量滴定板中孔之间的间距相匹配。 下部结构和支撑LED的上部结构之间的间隔足以使微量滴定板适合于它们之间。 电路按顺序发射单个LED并通过检测器收集光密度数据以用于微量滴定板孔中的样本。 然后将结构移动到微量滴定板上的下一个相邻的孔位置并重复该过程。

公开(公告)号：WO2010118185A3

公开(公告)日：2011-01-13

申请号：PCT/US2010030309

申请日：2010-04-07

Applicant: NEXUS DX INC ,  EGAN RICHARD ,  LIDGARD GRAHAM ,  BOOKER DAVID

Inventor： EGAN RICHARD ,  LIDGARD GRAHAM ,  BOOKER DAVID

IPC: G01N33/483 ,  G01N21/00 ,  G01N23/00 ,  G01N37/00

CPC classification number: G01N21/01 ,  G01N21/84 ,  G01N21/8483 ,  G01N33/487 ,  G01N2201/0446 ,  G01N2201/126 ,  G16H10/40 ,  Y02A90/26

Abstract: A diagnostic assay system including a test device and a scanning device are described. In one implementation, the scanning device includes a source of electromagnetic radiation, an optics assembly, a detector, and a microprocessor disposed within a chassis. The test device and scanning device may be configured to be movable relative to each other during operation of the scanning device.

Abstract translation: 描述了包括测试装置和扫描装置的诊断测定系统。 在一个实施方式中，扫描装置包括电磁辐射源，光学组件，检测器和布置在底盘内的微处理器。 测试装置和扫描装置可以被配置为在扫描装置的操作期间可相对于彼此移动。

公开(公告)号：WO2010122346A1

公开(公告)日：2010-10-28

申请号：PCT/GB2010/050661

申请日：2010-04-23

Applicant: AVACTA LIMITED ,  WEBSTER, Simon

Inventor： WEBSTER, Simon

IPC: G01N21/03

CPC classification number: G01N21/03 ,  G01N21/0332 ,  G01N2021/0346 ,  G01N2201/0446

Abstract: A unit is provided comprising an array (2) of sample containers (1), said containers, being connected together and arranged in a planar configuration, each container having multiple optically transparent windows arranged such that the sample contained therein can be interrogated using simultaneous multiple optical analytical techniques, the array of containers being configured so as to allow optical access to the windows of each container in the array. Also provided is an apparatus comprising such a unit, a system comprising a combination of such an apparatus and unit and a method of analysing multiple samples by introducing each individual sample into an individual container of such an apparatus, illuminating the samples and detecting and analysing light emerging therefrom.

Abstract translation: 提供了包括样品容器（1）的阵列（2），所述容器连接在一起并布置成平面构造的单元，每个容器具有多个光学透明窗口，其布置使得其中容纳的样品可以使用同时多个 光学分析技术，容器阵列被配置为允许光学访问阵列中每个容器的窗口。 还提供了一种包括这种单元的装置，包括这种装置和单元的组合的系统以及通过将每个单独的样品引入这种装置的单独容器中来分析多个样品的方法，照亮样品并检测和分析光 从中出现。

公开(公告)号：WO2006127191A9

公开(公告)日：2007-03-01

申请号：PCT/US2006015119

申请日：2006-04-20

Applicant: FLUIDIGM CORP ,  SUN GANG ,  HARRIS GREG ,  MAY ANDY ,  SELF KYLE ,  FARRELL KEVIN ,  WYATT PAUL

Inventor： SUN GANG ,  HARRIS GREG ,  MAY ANDY ,  SELF KYLE ,  FARRELL KEVIN ,  WYATT PAUL

IPC: G01N35/00

CPC classification number: G01N35/00029 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/10 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0829 ,  C12Q1/686 ,  G01N15/1475 ,  G01N21/6428 ,  G01N35/00732 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/0099 ,  G01N35/1065 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/00752 ,  G01N2035/0091 ,  G01N2035/1034 ,  G01N2201/025 ,  G01N2201/0446 ,  Y10S901/01 ,  Y10T436/11

Abstract: Systems for managing workflows to perform chemical or biological reactions using microfluidic devices.

Abstract translation: 管理工作流程以使用微流体装置进行化学或生物反应的系统。

公开(公告)号：WO2004104547A3

公开(公告)日：2005-03-24

申请号：PCT/US2004014566

申请日：2004-05-10

Applicant: MJ RES INC ,  KORDUNSKY IGOR ,  GOLDMAN JEFFREY A ,  FINNEY MICHAEL J

Inventor： KORDUNSKY IGOR ,  GOLDMAN JEFFREY A ,  FINNEY MICHAEL J

IPC: B01L7/00 ,  G01N21/64 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/686 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/1822 ,  G01N21/276 ,  G01N21/6452 ,  G01N2201/0446

Abstract: A fluorescence detection apparatus for analyzing samples located in a plurality of wells in a thermal cycler and methods of use are provided. In one embodiment, the apparatus includes a support structure attachable to the thermal cycler and a detection module movably mountable on the support structure. The detection module includes one or more channels, each having an excitation light generator and an emission light detector both disposed within the detection module. When the support structure is attached to the thermal cycler and the detection module is mounted on the support structure, the detection module is movable so as to be positioned in optical communication with different ones of the plurality of wells. The detection module is removable from the support structure to allow easy replacement.

Abstract translation: 提供了用于分析位于热循环仪中的多个孔中的样品的荧光检测装置和使用方法。 在一个实施例中，该装置包括可附接到热循环仪的支撑结构和可移动地安装在支撑结构上的检测模块。 检测模块包括一个或多个通道，每个通道具有设置在检测模块内的激发光发生器和发射光检测器。 当支撑结构附接到热循环仪并且检测模块安装在支撑结构上时，检测模块可移动以便定位成与多个井中的不同井的光通信。 检测模块可从支撑结构移除，以方便更换。

公开(公告)号：WO9426874A3

公开(公告)日：1995-01-19

申请号：PCT/US9405392

申请日：1994-05-13

Applicant: BAXTER DIAGNOSTICS INC

Inventor： WILLIAMS GREGORY ,  BROWN GARY ,  DANIEL CRAIG ,  OLSON CAROLYN ,  GARDNER WILLIAM ,  ENSCOE GLENN ,  JARRARD ELIZABETH

IPC: C12M1/34 ,  C12Q1/04 ,  G01N21/25 ,  G01N35/00 ,  C12N1/34

CPC classification number: G01N21/255 ,  C12M41/36 ,  C12M41/46 ,  G01N2035/00524 ,  G01N2201/0446 ,  G01N2201/064

Abstract: An improved optical system for use in an instrument for culturing and detecting the presence of microorganisms in human tissue specimens is disclosed. The improved optical detection system is designed to reduce unwanted noise by substantially blocking all light other than light from a sensor positioned on an inside wall of a culture bottle from reaching the photodetector. A control circuit providing a 'lock-in' detection system designed to reduce unwanted noise is also disclosed. The detection system is capable of rapidly and automatically detecting whether a specimen bottle has been inserted into the instrument.

公开(公告)号：WO1992022880A1

公开(公告)日：1992-12-23

申请号：PCT/US1992005105

申请日：1992-06-12

Applicant: ABBOTT LABORATORIES ,  KOTLARIK, John, J. ,  WROBLEWSKI, Mieczyslaw

Inventor： ABBOTT LABORATORIES

IPC: G06K09/00

CPC classification number: G01N35/109 ,  B01L3/0262 ,  B01L3/5085 ,  G01N15/1468 ,  G01N21/82 ,  G01N35/0095 ,  G01N35/0098 ,  G01N35/028 ,  G01N35/1004 ,  G01N2035/042 ,  G01N2035/0425 ,  G01N2035/0491 ,  G01N2035/1018 ,  G01N2035/1025 ,  G01N2035/1034 ,  G01N2201/0446 ,  G01N2201/0461 ,  G01N2201/0492 ,  G02B7/28 ,  Y10T137/7306 ,  Y10T436/115831

Abstract: A method for agglutination detection using optical imaging of reaction wells containing agglutination objects in a programmable random access automated apparatus for perforing agglutination assays is provided. An image of the agglutination objects in the reaction wells is taken with analyzation of the intensity values and transitions through relative portions of the image and background which provides intensity values suitable for classifying. The method utilizes slope information from a derivative (Fig. 34 A-E, second row) rather than absolute intensities, thus allowing scoring algorithm based on slope total information. The agglutination detection method uses a CCD camera to take an image at each well (Fig. 34 A-E, first row) of a multiple well cartridge, thus providing information suitable for analyzing from the image detection apparatus, the apparatus processes the information to generate a visual indication of the results of the assays being performed. A micro-processor is provided to assist in the operation of the apparatus as well as the image processing data collection and analysis.

Abstract translation: 本发明提供了一种用于穿孔凝集试验的可编程随机存取自动装置中使用含有凝集物的反应孔的光学成像的凝集检测方法。 通过分析强度值和通过图像和背景的相对部分的转变来获取反应井中的凝集物体的图像，其提供适于分类的强度值。 该方法利用来自导数（图34A-E，第二行）的斜率信息，而不是绝对强度，从而允许基于斜率总信息的评分算法。 凝集检测方法使用CCD照相机在多孔盒的每个孔（图34 AE，第一排）拍摄图像，从而提供适合于从图像检测装置进行分析的信息，该装置处理该信息以产生 正在进行测定结果的视觉指示。 提供微处理器以协助装置的操作以及图像处理数据的收集和分析。

公开(公告)号：WO1990003566A1

公开(公告)日：1990-04-05

申请号：PCT/EP1989001112

申请日：1989-09-23

Applicant: DYNATECH AG

Inventor： DYNATECH AG ,  NITTEL, Alfred

IPC: G01N21/59

CPC classification number: G01N21/253 ,  G01N2201/0407 ,  G01N2201/0446

Abstract: A reading device for a microtest plate (1) comprises a transport device which displaces the microtest plate (1) and a light unit (6) which emits light beams (100) that pass through a group of depressions (2a-2h) in the microtest plate (1) and enter a detector unit (7). A pulse generated in a detector (9) of the detector unit (7) is transmitted to a control and evaluation unit (45) and displayed. The reading unit consisting of the light unit (6) and the detector unit (7) is positioned obliquely to the direction of movement (150) of the microtest plate (1).

公开(公告)号：WO1983001111A1

公开(公告)日：1983-03-31

申请号：PCT/FI1982000036

申请日：1982-09-20

Applicant: EFLAB OY ,  VIRNES, Martti

Inventor： EFLAB OY

IPC: G01N21/64

CPC classification number: G01N21/6452 ,  G01N2021/6473 ,  G01N2201/0446

Abstract: An apparatus for the measurement of fluorescence out of a liquid sample, the said apparatus comprising a source (1) of light, from which the light of measurement is arranged to be passed vertically through optics (2), a monochromator (3), and through the sample placed in a pit plate (4) to a measurement monochromator (5) and a detector (6). According to the invention, for each process of measurement, the source (1) of light, the optics (2), the monochromator (3), the sample to be measured, the measurement monochromator (5), and the detector (6) are arranged so that they can be brought onto the same vertical straight line in relation to each other, in this sequence.

Abstract translation: 一种用于测量液体样品中的荧光的装置，所述装置包括光源（1），测量光从该光源发出，通过光源（2），单色仪（3）和 通过放置在凹坑板（4）中的样品到测量单色仪（5）和检测器（6）。 根据本发明，对于每个测量过程，光源（1），光学器件（2），单色仪（3），待测样品，测量单色仪（5）和检测器（6） 被布置成使得它们可以按照这个顺序相对于彼此被带到相同的垂直直线上。