公开(公告)号：CA2077560A1

公开(公告)日：1992-07-05

申请号：CA2077560

申请日：1991-12-23

Applicant: BAXTER DIAGNOSTICS INC

Inventor： MORRIS ROGER J ,  BASCOMB SHOSHANA ,  BOBOLIS JAMIE ,  SHERMAN DAVID ,  OLSON CAROLYN

IPC: C12M1/34 ,  C12Q1/06 ,  G01N20060101 ,  G01N21/27 ,  G01N21/64 ,  G01N21/78 ,  G01N21/80 ,  G01N33/49 ,  G01N33/52

Abstract: 2077560 9212413 PCTABS00014 The present invention is a multi-layer blood culture sensor which includes a pH sensitive absorbance dye encapsulated in or isolated by a first light transmissive gas permeable, proton impermeable matrix (2) and a pH insensitive fluorescence dye in a second matrix (4), the first and second matrices being spectrally coupled, and a method to use this sensor to detect microorganisms in a blood culture bottle.

公开(公告)号：DE69110658D1

公开(公告)日：1995-07-27

申请号：DE69110658

申请日：1991-10-30

Applicant: BAXTER DIAGNOSTICS INC

Inventor： MORRIS ROGER ,  BASCOMB SHOSHANA ,  OLSON CAROLYN

IPC: C12Q1/26 ,  G01N21/17 ,  G01N21/53 ,  G01N21/78

Abstract: A process to determine the concentration of any substance in a colorimetric, turbidimetric or nephelometric reaction using a fluorometric detector to measure fluorescence intensity. In particular, a change in color can be monitored by observing the measurement of fluorescence intensity of a fluorophore in an inert matrix. The absorption spectrum of the chromophore may overlap the excitation and/or emission spectrum of the fluorophore, thereby allowing the change in fluorescence to be related to the intensity of color in the reaction and thus related to the quantity of the substance of interest.

公开(公告)号：DE69110658T2

公开(公告)日：1996-03-21

申请号：DE69110658

申请日：1991-10-30

Applicant: BAXTER DIAGNOSTICS INC

Inventor： MORRIS ROGER ,  BASCOMB SHOSHANA ,  OLSON CAROLYN

IPC: C12Q1/26 ,  G01N21/17 ,  G01N21/53 ,  G01N21/78

Abstract: A process to determine the concentration of any substance in a colorimetric, turbidimetric or nephelometric reaction using a fluorometric detector to measure fluorescence intensity. In particular, a change in color can be monitored by observing the measurement of fluorescence intensity of a fluorophore in an inert matrix. The absorption spectrum of the chromophore may overlap the excitation and/or emission spectrum of the fluorophore, thereby allowing the change in fluorescence to be related to the intensity of color in the reaction and thus related to the quantity of the substance of interest.

公开(公告)号：WO9426874A3

公开(公告)日：1995-01-19

申请号：PCT/US9405392

申请日：1994-05-13

Applicant: BAXTER DIAGNOSTICS INC

Inventor： WILLIAMS GREGORY ,  BROWN GARY ,  DANIEL CRAIG ,  OLSON CAROLYN ,  GARDNER WILLIAM ,  ENSCOE GLENN ,  JARRARD ELIZABETH

IPC: C12M1/34 ,  C12Q1/04 ,  G01N21/25 ,  G01N35/00 ,  C12N1/34

CPC classification number: G01N21/255 ,  C12M41/36 ,  C12M41/46 ,  G01N2035/00524 ,  G01N2201/0446 ,  G01N2201/064

Abstract: An improved optical system for use in an instrument for culturing and detecting the presence of microorganisms in human tissue specimens is disclosed. The improved optical detection system is designed to reduce unwanted noise by substantially blocking all light other than light from a sensor positioned on an inside wall of a culture bottle from reaching the photodetector. A control circuit providing a 'lock-in' detection system designed to reduce unwanted noise is also disclosed. The detection system is capable of rapidly and automatically detecting whether a specimen bottle has been inserted into the instrument.