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    Multi-primer amplification method for barcoding of target nucleic acids
    132.
    发明授权
    Multi-primer amplification method for barcoding of target nucleic acids 有权

    公开(公告)号：US10344318B2

    公开(公告)日：2019-07-09

    申请号：US15590998

    申请日：2017-05-09

    Applicant: Fluidigm Corporation

    Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

    IPC: C12Q1/686 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00

    Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

    UNIVERSAL PROBE ASSAY METHODS
    140.
    发明申请
    UNIVERSAL PROBE ASSAY METHODS 审中-公开
    通用检测方法

    公开(公告)号：US20160348149A1

    公开(公告)日：2016-12-01

    申请号：US15144529

    申请日：2016-05-02

    Applicant: Fluidigm Corporation

    Inventor： Kenneth J. Livak ,  Jason A. A. West ,  Robert C. Jones

    IPC: C12Q1/68

    CPC classification number: C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/155 ,  C12Q2525/307 ,  C12Q2537/161

    Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

    Abstract translation: 提供了用于检测样品中靶多核苷酸的存在的试剂和方法。 一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并且将第一检测探针与扩增产物杂交，所述第一检测探针包含与第一探针结合序列杂交的第一区段和与第二探针结合序列杂交的第二区段，从而产生标记的扩增产物 。

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