公开(公告)号：WO2012099832A3

公开(公告)日：2012-07-26

申请号：PCT/US2012/021465

申请日：2012-01-17

Applicant: LIFE TECHNOLOGIES CORPORATION ,  HENDRICKS, Stephen

Inventor： HENDRICKS, Stephen

IPC: C12N15/10 ,  C12Q1/68

Abstract: Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.

公开(公告)号：WO2012097353A9

公开(公告)日：2012-07-19

申请号：PCT/US2012/021397

申请日：2012-01-14

Applicant: LIFE TECHNOLOGIES CORPORATION ,  DENG, David Xingfei ,  CHEN, Caifu

Inventor： DENG, David Xingfei ,  CHEN, Caifu

IPC: C12Q1/68 ,  G01N33/50

Abstract: Disclosed herein are methods for identifying rare cells containing particular markers and/or alleles from biological samples that have not been substantially pre-processed (e.g., unprocessed whole blood). The methods described herein provide a system for digital enrichment of target cells from a biological sample and detection of such target cells, thereby allowing accurate and efficient detection and / or enumeration of such cells in the sample.

公开(公告)号：WO2012083189A2

公开(公告)日：2012-06-21

申请号：PCT/US2011/065535

申请日：2011-12-16

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LI, Bin ,  LAO, Kai Qin ,  O'NEIL, Jennifer ,  KUNKEL, Jennifer ,  HALEY, Kellie ,  KASINSKAS, Rachel ,  MA, Zhaochun ,  BRZOSKA, Pius

Inventor： LI, Bin ,  LAO, Kai Qin ,  O'NEIL, Jennifer ,  KUNKEL, Jennifer ,  HALEY, Kellie ,  KASINSKAS, Rachel ,  MA, Zhaochun ,  BRZOSKA, Pius

IPC: C12Q1/68

CPC classification number: C12Q1/46 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.

Abstract translation: 提供了在支持物上产生固定化克隆扩增子的局部群体的新方法。

公开(公告)号：WO2012078796A1

公开(公告)日：2012-06-14

申请号：PCT/US2011/063805

申请日：2011-12-07

Applicant: LIFE TECHNOLOGIES CORPORATION ,  YOUNG, Jonathan R. ,  FREE, Kathleen E. ,  BROWNE, Elizabeth S.

Inventor： YOUNG, Jonathan R. ,  FREE, Kathleen E. ,  BROWNE, Elizabeth S.

IPC: G06K9/46

CPC classification number: G06T11/001 ,  G01N1/30 ,  G06K9/00127 ,  G06K9/0014 ,  G06K9/00201 ,  G06T7/0012 ,  G06T15/04 ,  G06T19/20 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2219/2012

Abstract: Systems and methods are used to display cell structures of a biological cell. A plurality of cell structures of a biological cell is stored and for each cell structure of the plurality of cell structures one or more stain colors are stored. A selected cell structure is received from an input device. One or more stain colors of the selected cell structure are retrieved. The one or more stain colors of the selected cell structure are displayed. A selected stain color is received from the input device. The selected cell structure is displayed in the selected stain color in an exemplary cell image. Further, a three-dimensional image of a biological cell is stored. The three-dimensional image is displayed on a display that includes a touch screen. A movement selection is received from the touch screen. The three-dimensional image is displayed on the display according to the movement selection.

Abstract translation: 系统和方法用于显示生物细胞的细胞结构。 存储生物细胞的多个细胞结构，并且对于多个细胞结构的每个细胞结构，存储一种或多种染色。 从输入设备接收所选择的单元结构。 检索所选择的细胞结构的一种或多种染色。 显示所选单元结构的一个或多个染色。 从输入装置接收选定的染色。 所选细胞结构以示例性细胞图像中所选择的染色显示。 此外，存储生物细胞的三维图像。 三维图像显示在包括触摸屏的显示器上。 从触摸屏接收移动选择。 根据移动选择，三维图像显示在显示器上。

公开(公告)号：WO2012068276A2

公开(公告)日：2012-05-24

申请号：PCT/US2011/061034

申请日：2011-11-16

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LEONG, Harrison ,  MAJUMDAR, Nivedita ,  SWARTZMAN, Elana

Inventor： LEONG, Harrison ,  MAJUMDAR, Nivedita ,  SWARTZMAN, Elana

IPC: G06F19/00

CPC classification number: G06F19/18 ,  G06F19/24

Abstract: A proximity binding assay (PBA) is performed on at least one test sample, at least one reference sample, a background sample, and one or more calibration samples using a thermal cycler instrument. Ct values are determined for at least one set of test sample data and at least one set of reference sample data. Background corrected Ct values are calculated using a corresponding value in a background sample data set. A linear range is determined for the background corrected Ct values as a function of sample quantity. A linear regression line is calculated for each linear range. One or more parameter values of an exponential model (EM) fold change formula are estimated from the one or more sets of calibration sample data. A target protein quantity and associated confidence interval are calculated using the linear regression lines and the EM fold change formula.

Abstract translation: 使用热循环仪在至少一个测试样品，至少一个参考样品，背景样品和一个或多个校准样品上进行接近结合测定（PBA）。 对于至少一组测试样本数据和至少一组参考样本数据确定Ct值。 使用背景样本数据集中的对应值计算背景校正的Ct值。 确定背景校正的Ct值作为样品量的函数的线性范围。 对于每个线性范围计算线性回归线。 从一组或多组校准样本数据估计指数模型（EM）倍数变化公式的一个或多个参数值。 使用线性回归线和EM倍数变化公式计算靶蛋白质量和相关置信区间。

公开(公告)号：WO2012058635A1

公开(公告)日：2012-05-03

申请号：PCT/US2011/058455

申请日：2011-10-28

Applicant: LIFE TECHNOLOGIES CORPORATION ,  YING, Lai-Qiang ,  YUE, Stephen ,  BRANCHAUD, Bruce ,  ZHANG, Yu-Zhong

Inventor： YING, Lai-Qiang ,  YUE, Stephen ,  BRANCHAUD, Bruce ,  ZHANG, Yu-Zhong

IPC: C12Q1/00

CPC classification number: C07D495/04 ,  C07B2200/11 ,  C07D403/12 ,  C07K1/13 ,  C07K1/22 ,  C07K16/42 ,  C07K2317/20 ,  C07K2317/92 ,  C08B37/00 ,  C09B11/24 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1088 ,  C12N5/0636 ,  C12N5/0637 ,  C12N5/0638 ,  G01N33/532 ,  G01N33/533 ,  G01N33/54353 ,  G01N33/58 ,  G01N33/582 ,  G01N33/6803

Abstract: Biotin derivatives, methods of using the biotin derivatives and kits comprising the biotin derivatives. In some embodiments, the biotin derivatives can be separated from streptavidin under less harsh conditions than are required for biotin. Accordingly, in some embodiments, the biotin derivatives are better suited for applications in which it would be desirable to dissociate the biotin derivative from streptavidin under less harsh conditions, such as to maintain the structural integrity and/or viability of the moiety attached to the biotin derivative.

Abstract translation: 生物素衍生物，使用生物素衍生物的方法和包含生物素衍生物的试剂盒。 在一些实施方案中，生物素衍生物可以在比生物素所需要的恶劣条件下与链霉抗生物素蛋白分离。 因此，在一些实施方案中，生物素衍生物更适合于在较不苛刻的条件下将生物素衍生物从链霉抗生物素蛋白解离的应用，例如维持连接于生物素的部分的结构完整性和/或存活力 衍生物。

公开(公告)号：WO2012006116A2

公开(公告)日：2012-01-12

申请号：PCT/US2011/042240

申请日：2011-06-28

Applicant: LIFE TECHNOLOGIES CORPORATION ,  BREVNOV, Maxim ,  BOLCHAKOVA, Elena ,  FURTADO, Manohar

Inventor： BREVNOV, Maxim ,  BOLCHAKOVA, Elena ,  FURTADO, Manohar

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6869 ,  C12Q2563/131 ,  C12Q2563/143

Abstract: A method for preparing a nucleic acid sample for nucleic acid sequencing includes amplifying a nucleic acid target sequence using a primer bound to a first capture substrate; capturing an amplified nucleic acid product by the first capture substrate; generating at least one sequencing ladder from the amplified nucleic acid product using at least one sequencing primer; capturing the at least one sequencing ladder by hybridizing the at least one sequencing ladder to a complementary capture compound on a second capture substrate; and removing the at least one sequencing ladder from the second capture substrate. The first and/or second capture substrate may include a magnetic particle. Other methods, workflows, kits, and computer program media for nucleic acid sample preparation are also disclosed.

Abstract translation: 用于制备用于核酸测序的核酸样品的方法包括使用与第一捕获底物结合的引物扩增核酸靶序列; 通过第一捕获基质捕获扩增的核酸产物; 使用至少一个测序引物从扩增的核酸产物产生至少一个测序梯; 通过将所述至少一个测序梯度杂交到第二捕获基底上的互补捕获化合物来捕获所述至少一个测序梯; 以及从所述第二捕获基板去除所述至少一个测序梯。 第一和/或第二捕获基板可以包括磁性颗粒。 还公开了用于核酸样品制备的其它方法，工作流程，试剂盒和计算机程序培养基。

公开(公告)号：WO2012006084A3

公开(公告)日：2012-01-12

申请号：PCT/US2011/042176

申请日：2011-06-28

Applicant: LIFE TECHNOLOGIES CORPORATION ,  DANEHY, Ronald ,  FONSECA, Jorge ,  PRASAD, Jayanthi

Inventor： DANEHY, Ronald ,  FONSECA, Jorge ,  PRASAD, Jayanthi

IPC: G01N35/10 ,  G01N33/48 ,  B01L3/02 ,  C12Q1/68

Abstract: A system for preparing biological sample contains a body including a proximal side and a distal side, a plurality of mandrels, a plurality of resilient elements, a plurality of fluid dispensers, and one or more samples. The mandrels are moveably positioned within the body, where each resilient element engages a respective one of the mandrels. Each of the fluid dispensers is configured to engage a distal end of a corresponding one of the mandrels. Each sample comprises a solution containing one or more nucleic acid sequences contained within at least one of the fluid dispensers.

公开(公告)号：WO2012003380A3

公开(公告)日：2012-01-05

申请号：PCT/US2011/042683

申请日：2011-06-30

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LEVINE, Peter

Inventor： LEVINE, Peter

IPC: H01L29/772 ,  H01L21/50

Abstract: The described embodiments may provide a chemical detection circuit with an improved signal-to-noise ration. The chemical detection circuit may include a current source, a chemical detection pixel, an amplifier and a capacitor. The chemical detection pixel may comprise a chemical-sensitive transistor that may have a first and second terminals and a row-select switch coupled between the current source and chemically- sensitive transistor. The amplifier may have a first input and a second input, with the first input coupled to an output of the chemically-sensitive transistor via a switch and the second input coupled to an offset voltage line. The capacitor may be coupled between an output of the amplifier and the first input of the amplifier. The capacitor and amplifier may form an integrator and may be shared by a column of chemical detection pixels.

公开(公告)号：WO2011163120A1

公开(公告)日：2011-12-29

申请号：PCT/US2011/041061

申请日：2011-06-20

Applicant: LIFE TECHNOLOGIES CORPORATION ,  WOO, Cora ,  BERKMAN, Jennifer ,  YIM, Priscilla

Inventor： WOO, Cora ,  BERKMAN, Jennifer ,  YIM, Priscilla

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6848 ,  C12Q2549/125 ,  C12Q2527/125 ,  C12Q2521/107 ,  C12Q2537/101

Abstract: The present invention is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in a one-step RT-PCR procedure comprising one or more agents used to increase tolerance to PCR inhibitors.

Abstract translation: 本发明涉及可用于合成核酸分子的组合物，方法和试剂盒。 更具体地，提供组合物，方法和试剂盒，用于在包括一种或多种用于增加对PCR抑制剂的耐受性的试剂的一步RT-PCR方法中扩增核酸分子。