公开(公告)号：WO1995034320A2

公开(公告)日：1995-12-21

申请号：PCT/US1995007351

申请日：1995-06-07

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  BLAZAR, Bruce, R. ,  VALLERA, Daniel, A.

IPC: A61K39/00

CPC classification number: C07K14/70521 ,  A61K38/00 ,  C07K16/247 ,  C07K16/2827 ,  C07K16/2845 ,  C07K16/2866 ,  C07K2319/00

Abstract: Methods for inhibiting antigen-specific T cell responses by use of an agent which inhibits a costimulatory signal in T cells are disclosed. Preferably, both a first agent which inhibits a costimulatory signal in the T cell (e.g., a CTLA4Ig fusion protein) and a second agent which inhibits another T cell function, such as adhesion of the T cell to a cell presenting antigen to the T cell, are used to inhibit antigen-specific T cell responses. For example, to inhibit adhesion of a T cell to a cell presenting antigen, an anti-LFA-1 antibody can be used in conjunction with a CTLA4Ig fusion protein. Alternatively, another agent which inhibits a costimulatory signal in T cells, such as an anti-B7-1 antibody or an anti-B7-2 antibody can be used with a second agent which inhibits a proliferative signal in the T cell e.g., an anti-IL-2 receptor antibody. The methods of the invention are particularly useful for inhibiting graft versus host disease and for inhibiting rejection of a transplanted tissue or organ.

Abstract translation: 公开了通过使用抑制T细胞共刺激信号的药剂来抑制抗原特异性T细胞应答的方法。 优选地，抑制T细胞中共刺激信号的第一药剂（例如，CTLA4Ig融合蛋白）和抑制另一T细胞功能的第二药剂，例如T细胞向T细胞呈递抗原的粘附 ，用于抑制抗原特异性T细胞应答。 例如，为了抑制T细胞与细胞呈递抗原的粘附，可以将抗LFA-1抗体与CTLA4Ig融合蛋白结合使用。 或者，抑制T细胞中的共刺激信号（例如抗B7-1抗体或抗B7-2抗体）的另一种试剂可以与抑制T细胞增殖信号的第二种试剂一起使用，例如抗 - -IL-2受体抗体。 本发明的方法对于抑制移植物抗宿主病和抑制移植组织或器官的排斥特别有用。

公开(公告)号：WO1995031227A1

公开(公告)日：1995-11-23

申请号：PCT/US1995005939

申请日：1995-05-08

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  CLAGUE, Cynthia, T. ,  Blackshear, Perry, L., Jr.

IPC: A61M01/00

CPC classification number: A61M1/0084 ,  A61M1/0058 ,  A61M1/0076 ,  A61M1/3496 ,  A61M1/3626

Abstract: A probe (60) for use in collecting liquids entrained with a gaseous component, particularly collecting a patient's blood with entrained gas bubbles from a surgical site for autologous reinfusion. The probe separates the gaseous component from the collected liquid, particularly separating air bubbles from blood cells to inhibit hemolysis, platelet degradation and protein denaturation. An elongated probe body (60) has a distal end to be inserted into the liquid to be collected through an inlet (91) by jet pump action into a centrally disposed mixing chamber (76). A diffuser (78) is formed within the probe body (60) proximal to and in axial alignment with the inlet (91) and mixing chamber (76) leading to a liquid exit. A jet pump nozzle (90) is formed in the probe distal tip surrounding the inlet in the form of an annular chamber into which pressurized drive fluid is introduced through a distribution channel system extending along the probe body (60) from the proximal end.

Abstract translation: 用于收集夹带有气体成分的液体的探针（60），特别是用于自动回输的外科手术部位带有夹带气泡的患者血液。 探针从收集的液体中分离气体成分，特别是从血细胞中分离气泡，以抑制溶血，血小板降解和蛋白质变性。 细长的探针主体（60）具有远端，以通过入口（91）插入待收集的液体中，通过射流泵作用进入中心设置的混合室（76）。 扩散器（78）形成在探针主体（60）内，并与入口（91）和混合室（76）轴向对齐并通向液体出口。 喷射泵喷嘴（90）形成在环形腔室形式的围绕入口的探针远侧末端中，加压驱动流体通过从近端沿着探针体（60）延伸的分配通道系统被引入到喷嘴泵喷嘴（90）中。

公开(公告)号：WO1995017565A1

公开(公告)日：1995-06-29

申请号：PCT/US1994012481

申请日：1994-10-31

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  ERICKSON, Robert, W.

IPC: E04C03/36

CPC classification number: E04C3/122 ,  B27M3/006 ,  B27M3/32 ,  E04C3/127 ,  E04C3/36 ,  Y10S52/08

Abstract: A hollow light-weight pole-like structure (10) suitable for use as a utility pole, column or log building member is made of a plurality of wood strips (12) glued edge to edge forming a hollow interior core (22), with at least one layer of high strength veneer material (26, 28, 30) glued to the exterior of the wood strips with the veneer grain running parallel to the length. The layer of veneer (26, 28, 30) increases the strength, distributes bending stresses uniformly, provides greater decay resistance than ordinary glued hollow poles, and increases the service life. The veneer (26, 28, 30) can be made in several layers (26, 28, 30) forming a continuous overwrap without longitudinally extending gaps that expose the interior wood strips (12) to weather or splitting.

Abstract translation: 适合用作电杆，立柱或木建筑构件的空心轻型杆状结构（10）由形成中空内部芯（22）的边缘到边缘的多个木条（12）制成，其中 至少一层高强度的单板材料（26,28,30），其胶合到木条的外部，单板颗粒与该长度平行延伸。 薄板层（26,28,30）增强强度，均匀分布弯曲应力，比普通胶合中空杆提供更大的抗衰减性能，并延长使用寿命。 单板（26,28,30）可以制成几层（26,28,30），形成连续的外包装，而不会使内部木条（12）暴露或分裂的纵向延伸间隙。

公开(公告)号：WO1995013071A3

公开(公告)日：1995-05-18

申请号：PCT/US1994012844

申请日：1994-11-08

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA ,  MINNEAPOLIS MEDICAL RESEARCH FOUNDATION

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  MINNEAPOLIS MEDICAL RESEARCH FOUNDATION ,  PORTOGHESE, Philip, S. ,  SHARP, Burt, M. ,  LINNER, Kristin, M.

IPC: A61K31/485

公开(公告)号：WO1995009926A1

公开(公告)日：1995-04-13

申请号：PCT/US1994011279

申请日：1994-10-06

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA ,  SHERMAN, David, H. ,  AUGUST, Paul, R. ,  FLICKINGER, Michael, C.

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA

IPC: C12Q01/18

CPC classification number: C12N9/0004 ,  C07K14/36 ,  C12N15/52 ,  C12Q1/6827

Abstract: The invention provides for an expression cassette including a DNA sequence that provides resistance to a cell to a DNA bioreductive alkylating or cleaving agent. DNA bioreductive alkylating or cleaving agents include mitomycins and enediynes. The expression cassette can provide resistant transformed cells, resistance polypeptides, and DNA probes and primers. The invention also provides methods for identifying agents that inhibit cellular resistance to agents such as mitomycins. DNA probes and polymerase chain reaction primers can be used in a method to identify homologous DNA sequences in other organisms and/or cell types.

Abstract translation: 本发明提供了一种表达盒，其包括提供对细胞对DNA生物还原性烷基化或切割剂的抗性的DNA序列。 DNA生物还原性烷基化或切割剂包括丝裂霉素和烯二炔。 表达盒可以提供抗性转化细胞，抗性多肽和DNA探针和引物。 本发明还提供用于鉴定抑制细胞对诸如丝裂霉素的药物抗性的药剂的方法。 DNA探针和聚合酶链反应引物可用于鉴定其他生物体和/或细胞类型中的同源DNA序列的方法。

公开(公告)号：WO1994017198A1

公开(公告)日：1994-08-04

申请号：PCT/US1994000810

申请日：1994-01-21

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  LURIE, Keith, G. ,  WIEGM, Phi

IPC: C12Q01/00

CPC classification number: C12Q1/00 ,  C12Q1/26 ,  C12Q1/34 ,  C12Q1/40 ,  C12Q1/44 ,  C12Q1/48 ,  C12Q1/527 ,  G01N2333/988 ,  Y10S435/963

Abstract: A method of measuring adenylate cyclase (AC) in a sample of physiological material which does not employ radioactive reagents is provided, comprising: (a) providing a physiological sample of physiological material comprising cAMP produced by endogenous AC, and other endogenous adenine nucleotides selected from the group consisting of ATP, AMP, ADP and mixtures thereof; (b) combining said sample with (a) effective amounts of apyrase, 5'-nucleotidase and adenosine deaminase, so as to enzymatically eliminate said other endogenous adenine nucleotides in said sample and (b) an amount of alkaline phosphatase effect to eliminate the glucose-6-phosphate in said sample; (c) enzymatically converting the cAMP into AMP; and (d) measuring the amount of AMP, said amount providing a measure of the amount of cAMP and AC in said sample.

Abstract translation: 提供了一种在不使用放射性试剂的生理材料样品中测量腺苷酸环化酶（AC）的方法，其包括：（a）提供包含由内源性AC产生的cAMP和其它内源腺嘌呤核苷酸的生理学物质的生理样品， 由ATP，AMP，ADP及其混合物组成的组; （b）将所述样品与（a）有效量的apyrase，5'-核苷酸酶和腺苷脱氨酶组合，以便酶促消除所述样品中的所述其它内源腺嘌呤核苷酸，和（b）一定量的碱性磷酸酶作用以消除葡萄糖 所述样品中的磷酸-6-磷酸; （c）将cAMP酶促转化为AMP; 和（d）测量AMP的量，所述量提供所述样品中cAMP和AC的量的量度。

公开(公告)号：WO1994011045A1

公开(公告)日：1994-05-26

申请号：PCT/US1993011034

申请日：1993-11-15

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  LURIE, Keith, G. ,  GOLD, Barbara, S.

IPC: A61M15/00

CPC classification number: A61K38/11 ,  A61K31/21 ,  A61K45/06 ,  A61K31/135

Abstract: Methods and pharmaceutical compositions for resuscitating patients suffering from cardiac arrest are disclosed. The methods comprise the performance of cardiopulmonary resuscitation techniques which result in active inducement of venous blood transport into the heart and arterial blood transport from the heart. During the performance of such techniques, the patient is administered with an amount of an arterial constrictor sufficient to increase arterial blood pressure and with an amount of a venodilator sufficient to enhance arterial blood flow to the brain and heart. Pharmaceutical compositions comprise both the arterial constrictor and venodilator present in a single formulation. The methods are found to both enhance patient survival and reduce heart and brain damage.

Abstract translation: 公开了用于复苏心脏骤停患者的方法和药物组合物。 这些方法包括进行心肺复苏技术，导致静脉输血进入心脏的心脏和动脉血液输送的积极诱导。 在执行这种技术期间，向患者施用足以增加动脉血压的量的动脉缩窄物，以及足以增加动脉血流到脑和心脏的静脉扩张剂的量。 药物组合物包含存在于单一制剂中的动脉缩窄物和静脉扩张剂。 发现这些方法既能增强患者的生存能力，又能减轻心脑损伤。

公开(公告)号：WO1994007114A1

公开(公告)日：1994-03-31

申请号：PCT/US1993008764

申请日：1993-09-16

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  ROBBINS, William, P. ,  MUELLER, Rolf, K.

IPC: G01H01/00

CPC classification number: G01N29/14 ,  A01M1/026 ,  A01M2200/011 ,  G01H11/08 ,  G01N2291/101 ,  G01V1/001

Abstract: A system (10) for detecting wood-destroying insects by sensing acoustic emissions generated by the insects as they feed. The system comprises two acoustic emission sensors (14, 15), an amplification section (16), a signal processing section (18) and an indicator section (20). The system (10) comes into mechanical contact with the wood (24) to be inspected through the use of a bolt (22) which is inserted into the wood (12) and attaches to an acoustic emission sensor (14) or through the use of an adhesive layer (47) which directly attaches an acoustic emission sensor (15) to the wood. The acoustic emission sensors (14, 15) are electrically connected to the amplification section (16) which is electrically connected to the signal processing section (18). The signal processing section (18) is capable of distinguishing between insect-caused acoustic emissions and noise-caused acoustic emissions detected by the acoustic emission sensors (14, 15). The results of the signal processing section are sent to the indicator section (20).

Abstract translation: 一种用于通过感测昆虫在饲料中产生的声发射来检测木材破坏昆虫的系统（10）。 该系统包括两个声发射传感器（14,15），放大部分（16），信号处理部分（18）和指示器部分（20）。 系统（10）通过使用插入到木材（12）中的螺栓（22）并附接到声发射传感器（14）或通过使用的螺栓（22）与待检查的木材（24）进行机械接触 的粘合剂层（47），其将声发射传感器（15）直接附接到木材上。 声发射传感器（14,15）电连接到电连接到信号处理部分（18）的放大部分（16）。 信号处理部分（18）能够区分由声发射传感器（14,15）检测到的昆虫引起的声发射和噪声引起的声发射。 信号处理部分的结果被发送到指示器部分（20）。

公开(公告)号：WO1993023456A1

公开(公告)日：1993-11-25

申请号：PCT/US1993002626

申请日：1993-03-23

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  VAIDYA, Utpal, R. ,  BHATTACHARYA, Mrinal

IPC: C08G81/02

CPC classification number: C08L101/16 ,  B29K2003/00 ,  C08B31/00 ,  C08G81/00 ,  C08G81/02 ,  C08H1/00

Abstract: A biodegradable interpolymer and composition is prepared by the reactive blending of a synthetic polymer having functional groups with a naturally occurring biodegradable polymer such as a carbohydrate or protein compound. During reactive blending, the synthetic polymer can undergo a chemical reaction with the biodegradable natural polymer which results in covalent and physical bonding between the two polymers, thereby forming an interpolymer. By this process, a biodegradable interpolymer composition is produced that is suitable for molding various articles.

Abstract translation: 通过将具有官能团的合成聚合物与天然存在的可生物降解的聚合物如碳水化合物或蛋白质化合物的反应性共混来制备可生物降解的互聚物和组合物。 在反应混合过程中，合成聚合物可以与可生物降解的天然聚合物发生化学反应，导致两种聚合物之间的共价和物理结合，从而形成互聚物。 通过该方法，生产适合于模制各种制品的可生物降解的互聚物组合物。

公开(公告)号：WO1993020184A1

公开(公告)日：1993-10-14

申请号：PCT/US1993002563

申请日：1993-03-19

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  MCGLAVE, Philip, B. ,  VERFAILLIE, Catherine, M.

IPC: C12N05/00

CPC classification number: C12N5/0647 ,  C12N2501/21 ,  C12N2501/23 ,  C12N2501/39 ,  C12N2502/1394

Abstract: A method for culturing mammalian hematopoietic, preferably stem cells, is provided comprising maintaining a population of human hematopoietic cells in a non-contacting relationship to a population of cultured stromal cells, which populations are in preferably liquid stromal growth medium connection, so that the ability of the stem cells in said population to differentiate and self-replicate is maintained during an extended culture period.

Abstract translation: 提供了一种用于培养哺乳动物造血干细胞的方法，优选干细胞包括维持人体造血细胞群与培养基质细胞群的非接触关系，所述群体优选为液体基质生长培养基连接， 的干细胞在所述群体中进行分化和自我复制是在延长的培养期间保持的。