公开(公告)号：WO2011143611A2

公开(公告)日：2011-11-17

申请号：PCT/US2011/036516

申请日：2011-05-13

Applicant: LIFE TECHNOLOGIES CORPORATION ,  BROOMER, Adam ,  LI, Kelly ,  TOBLER, Andreas ,  CHEN, Caifu ,  KEYS, Jr., David

Inventor： BROOMER, Adam ,  LI, Kelly ,  TOBLER, Andreas ,  CHEN, Caifu ,  KEYS, Jr., David

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/6844 ,  C12Q2537/16

Abstract: This disclosure relates to methods and kits for karyotyping in which chromosomes are interrogated by amplifying loci that are not within copy number variable regions thereof.

Abstract translation: 本公开涉及用于核型分析的方法和试剂盒，其中染色体通过扩增不在其拷贝数可变区内的位点进行询问。

公开(公告)号：WO2011137368A3

公开(公告)日：2011-11-03

申请号：PCT/US2011/034611

申请日：2011-04-29

Applicant: LIFE TECHNOLOGIES CORPORATION ,  ZHANG, Zheng ,  GUO, Danwei ,  LOU, Yuandan ,  BRINZA, Dumitru

Inventor： ZHANG, Zheng ,  GUO, Danwei ,  LOU, Yuandan ,  BRINZA, Dumitru

IPC: G06F19/22 ,  G06F19/28 ,  C12Q1/68

Abstract: Nucleic acid sequence mapping/assembly methods are disclosed. The methods initially map only a contiguous portion of each read to a reference sequence and then extends the mapping of the read at both ends of the mapped contiguous portion until the entire read is mapped (aligned). In various embodiments, a mapping score can be calculated for the read alignment using a scoring function, score (i, j) = M+mx, where M can be the number of matches in the extended alignment, x can be the number of mismatches in the alignment, and m can be a negative penalty for each mismatch. The mapping score can be utilized to rank or choose the best alignment for each read.

公开(公告)号：WO2011130184A3

公开(公告)日：2011-10-20

申请号：PCT/US2011/031988

申请日：2011-04-11

Applicant: LIFE TECHNOLOGIES CORPORATION ,  GEORGE, Wallace R.

Inventor： GEORGE, Wallace R.

IPC: G06F19/12 ,  G06F19/28 ,  G06F17/50 ,  C12Q1/68 ,  G01N33/50

Abstract: A method for determining a cycle threshold for a PCR amplification curve is provided. The method includes receiving a data set for a plurality of biological samples for a PCR amplification reaction. The data set includes a plurality of amplification curves, each amplification curve associated with a biological sample of the plurality of biological samples. The method further includes performing a nonlinear optimization comprising a fit of each amplification curve to a complementary modeled amplification curve to determine a best-fit set of parameters for a modeled efficiency curve and associated amplification curve. The modeled amplification curve is based on a modeled efficiency curve. The method includes determining a cycle threshold value for each biological sample based on a complementary relationship of the modeled efficiency curve to the modeled amplification curve. In an embodiment, the nonlinear optimization is a constrained nonlinear optimization.

公开(公告)号：WO2011106634A2

公开(公告)日：2011-09-01

申请号：PCT/US2011/026228

申请日：2011-02-25

Applicant: LIFE TECHNOLOGIES CORPORATION ,  DAVIDSON, John, F. ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan ,  WHITAKER, Richard

Inventor： DAVIDSON, John, F. ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan ,  WHITAKER, Richard

IPC: C12Q1/68 ,  C12Q1/48 ,  C12N9/12

CPC classification number: C12N9/1252

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

Abstract translation: 提供了包含修饰的蛋白质，特别是具有改变的缓冲性质的修饰的核酸结合蛋白的方法，组合物，系统，装置和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰的修饰蛋白以实现期望的pKa值的方法。 此外，本发明提供了在离子生成反应中使用这些修饰蛋白的方法，如基于离子的核酸测序反应。

公开(公告)号：WO2011072078A3

公开(公告)日：2011-06-16

申请号：PCT/US2010/059573

申请日：2010-12-08

Applicant: LIFE TECHNOLOGIES CORPORATION ,  ADAIR, Nicholas ,  CRESPO, Joselito ,  RUSSELL, Paul ,  PERRY, Allen

Inventor： ADAIR, Nicholas ,  CRESPO, Joselito ,  RUSSELL, Paul ,  PERRY, Allen

IPC: B65D75/34 ,  B65D85/30 ,  B65D77/26 ,  G01N1/00

Abstract: A packaging system for transporting vials containing biological samples may comprise a first tray defining at least one first tray cavity; and a second tray defining at least one second tray cavity and configured to mate with the first tray. The packaging system may further comprise at least one first tray cavity and at least one second tray cavity, wherein the at least one first tray cavity and the at least one second tray cavity are configured to securely hold respective vials for transport, and to restrain caps on the respective vials during transport, wherein the at least one first tray cavity and the at least one second tray cavity oppose each other when the first tray and the second tray are mated together. The packaging system may also be configured to permit barcode scanning of vials held within the first tray cavity and the second tray ca

公开(公告)号：WO2011050340A1

公开(公告)日：2011-04-28

申请号：PCT/US2010/053873

申请日：2010-10-22

Applicant: LIFE TECHNOLOGIES CORPORATION ,  SIKORA, Marcin ,  BLANCHARD, Alan

Inventor： SIKORA, Marcin ,  BLANCHARD, Alan

IPC: C12Q1/68

CPC classification number: G06F19/20 ,  C12Q1/6869 ,  C12Q1/6874 ,  G06F19/10 ,  G06F19/22 ,  C12Q2565/102 ,  C12Q2521/501

Abstract: Disclosed are systems and methods for polynucleotide sequencing where detection and correction of base calling errors can be achieved without reliance on a reference sequence. In certain embodiments, redundant information, which may be provided by additional labels, can be introduced during measurement so as to allow such detection of errors. Such redundant information and measurements can be facilitated by encoding of nucleotide sequence being measured. Various examples of such encoding, redundancy introduction, and decoding are provided.

Abstract translation: 公开了用于多核苷酸测序的系统和方法，其中可以在不依赖参考序列的情况下实现基本呼叫错误的检测和校正。 在某些实施例中，可以在测量期间引入可由附加标签提供的冗余信息，以允许这种错误的检测。 可以通过编码被测量的核苷酸序列来促进这种冗余信息和测量。 提供了这种编码，冗余引入和解码的各种示例。

公开(公告)号：WO2011046972A2

公开(公告)日：2011-04-21

申请号：PCT/US2010/052387

申请日：2010-10-12

Applicant: LIFE TECHNOLOGIES CORPORATION ,  SCHROEDER, Benjamin ,  WENZ, Michael

Inventor： SCHROEDER, Benjamin ,  WENZ, Michael

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6848 ,  C12Q2527/127

Abstract: The present disclosure generally provides compositions, methods and kits for reducing unwanted primer interactions (e.g., primer dimer structure formation). More specifically, the disclosure provides for compositions, methods and kits for reducing non-specific side products and/or interactions resulting from primer dimer formation prior to or during amplification of target nucleic acids.

Abstract translation: 本公开通常提供用于减少不想要的引物相互作用（例如引物二聚体结构形成）的组合物，方法和试剂盒。 更具体地，本公开提供了用于在靶核酸扩增之前或期间减少由引物二聚体形成引起的非特异性副产物和/或相互作用的组合物，方法和试剂盒。

公开(公告)号：WO2011040933A1

公开(公告)日：2011-04-07

申请号：PCT/US2009/059564

申请日：2009-10-05

Applicant: MDS ANALYTICAL TECHNOLOGIES ,  LIFE TECHNOLOGIES CORPORATION ,  BLOOMFIELD, Nic G. ,  IVOSEV, Gordana

Inventor： BLOOMFIELD, Nic G. ,  IVOSEV, Gordana

IPC: G01D18/00 ,  G12B13/00

CPC classification number: H01J49/0009

Abstract: Reference features are updated based on a previous scan during each mass spectrometry scan of a sample. Reference features with reference feature confidence values are generated from a plurality of initial scans. For each subsequent scan of a sample, sample features and sample feature confidence values are calculated. The reference features and sample features are aligned to determine common features. Constants are determined for an equation of mass of the mass spectrometer using confidence weighted regression of the common features. The constants and the equation of mass are used to calculate new mass values for the sample features. The reference features are updated using the sample features and the reference feature confidence values are recalculated in order to maintain the accuracy of reference features for calibration.

Abstract translation: 基于在样品的每次质谱扫描期间的先前扫描来更新参考特征。 从多个初始扫描生成具有参考特征置信度的参考特征。 对于样本的每个后续扫描，计算样本特征和样本特征置信度值。 参考特征和样本特征对齐以确定共同特征。 使用公共特征的置信加权回归确定质谱方程式的常数。 常数和质量方程用于计算样本特征的新质量值。 参考特征使用示例特征进行更新，参考特征重新计算置信度值，以保持校准参考特征的准确性。

公开(公告)号：WO2011032054A3

公开(公告)日：2011-03-17

申请号：PCT/US2010/048542

申请日：2010-09-10

Applicant: LIFE TECHNOLOGIES CORPORATION ,  FANG, Rixun ,  FURTADO, Manohar ,  KAYSER, Manfred

Inventor： FANG, Rixun ,  FURTADO, Manohar ,  KAYSER, Manfred

IPC: C12Q1/68 ,  C12N15/12 ,  C40B40/08

Abstract: The methods and compositions provided herein relate to the discovery of 13 STR are markers found on the human Y chromosome with surprisingly high mutation rates when compared with 173 other Y-STR markers known today, including those that are in common use in forensics. In addition to theoretical expectations based on elevated mutation rates, these 13 rapidly-mutating (RM) Y-STRs proved to be suitable for differentiating between closely related males, as well as between more distant male relatives and much more so than the most-commonly used Y-STRs in forensics. Our new set of RM-Y-STRs is expected to overcome the current dilemma of Y-chromosome analysis in forensic applications from current male lineage identification towards male individual identification in many cases, due to their extraordinary mutation properties as discovered here. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.

Abstract translation: 本文提供的方法和组合物涉及发现13个STR是在人类Y染色体上发现的标记，与当前已知的173种其他Y-STR标记相比，具有令人惊讶的高突变率，包括那些 在法医学中常用。 除了基于提高的突变率的理论预期之外，这13个快速突变（RM）Y-STR被证明适用于区分密切相关的雄性以及更远距离的男性亲属之间，以及最常见的 在取证中使用了Y-STR。 由于在这里发现的非凡突变特性，我们的新一套RM-Y-STRs预计将克服当前男性谱系鉴定到男性个体鉴定的法医应用中Y染色体分析的困境。 本发明的实施方案包括用于快速突变的Y-STR标志物的等位基因测定的方法，用于快速突变的Y-STR标志物的分析的扩增引物，用于分析快速突变的Y-STR标志物的等位基因梯级以及用于分析的试剂盒 的快速突变的Y-STR标记。

公开(公告)号：WO2011031497A2

公开(公告)日：2011-03-17

申请号：PCT/US2010/046695

申请日：2010-08-25

Applicant: LIFE TECHNOLOGIES CORPORATION ,  DILLER, Thomas ,  UPDYKE, Timothy

Inventor： DILLER, Thomas ,  UPDYKE, Timothy

IPC: G01N33/68 ,  G01N33/52 ,  G01N33/58

CPC classification number: G01N33/6803 ,  B33Y80/00 ,  C07K1/26 ,  Y10T436/10 ,  Y10T436/105831

Abstract: The present invention provides dual labeled protein standards useful for the simultaneous determination of the molecular weight of a subject protein as well as the relative mass (i.e., amount) of the subject protein present in an electrophoresis lane. The invention is also directed to methods suitable for the preparation of such dual labeled protein standards and to methods of using such dual labeled proteins to simultaneously determine the molecular weight and the relative amount of a subject protein. Further embodiments are directed to the use dual labeled protein standards to make a more accurate determination of the amount of a protein present in an electrophoresis lane. Yet further embodiments are directed to kits containing the presently described dual protein standards. Dual labeled protein standards made and used in accordance with the embodiments set forth herein may be used to simultaneously determine the molecular weight and the relative amount of a subject protein in real time.

Abstract translation: 本发明提供了用于同时测定受试蛋白的分子量以及存在于电泳泳道中的受试蛋白的相对质量（即，量）的双重标记蛋白质标准。 本发明还涉及适用于制备这种双重标记的蛋白质标准品的方法以及使用这种双标记蛋白质同时测定受试蛋白质的分子量和相对量的方法。 进一步的实施方案涉及使用双重标记的蛋白质标准物来更准确地测定电泳泳道中存在的蛋白质的量。 另外的实施方案涉及包含目前描述的双重蛋白质标准品的试剂盒。 根据本文所述的实施方案制备和使用的双重标记的蛋白质标准物可用于同时确定受试蛋白质的分子量和相对量。