公开(公告)号：EP0213628A3

公开(公告)日：1988-09-21

申请号：EP86112075

申请日：1986-09-01

Applicant: YEDA RESEARCH AND DEVELOPMENT COMPANY, LTD.

Inventor： Groner, Yoram Meonot Wolfson A.

IPC: C12N15/00 ,  C12N09/02 ,  C12N05/00

CPC classification number: C12N9/0089 ,  C12N15/85

Abstract: The invention concerns the production of superoxide dismutase (SOD) by eucaryotic cells derived from multicellular organisms which are capable of expressing a DNA sequence encoding for superoxide dismutase and producing the superoxide dismutase or analogs thereof. A specific embodiment of the invention includes a human HeLa cell or mouse L cell containing either the pmSV2-SOD-Neo plasmid or the pHG-SOD-SV-Neo plasmid. The pmSV2-SOD-Neo plasmid contains a DNA sequence encoding for superoxide dismutase joined to the SV40 early promoter. The pHG-SOD-SV-Neo plasmid contains a DNA sequence encoding for superoxide dismutase controlled by the native regulatory elements of human genomic Cu-Zn SOD. Such cells are capable of expressing the sequence encoding the superoxide dismutase and of producing the superoxide dismutase or an analog thereof. The invention also concerns methods of producing the superoxide dismutase by growing the cells of the invention and recovering the superoxide dismutase product.

公开(公告)号：EP0254305A1

公开(公告)日：1988-01-27

申请号：EP87110642.3

申请日：1987-07-22

Applicant: YEDA RESEARCH AND DEVELOPMENT COMPANY, LTD.

Inventor： Daren, Stephen L. J. ,  Vofsi, David ,  Finkels, Aurelia

IPC: C07C17/34 ,  C07C25/28

CPC classification number: C07C17/25 ,  C07C25/28

Abstract: The invention relates to an improved process for the production of crystalline tribromostyrene. The process involves removal of hydrogen bromide in a two phase system with the aid of phase transfer catalysts. One of the phases used is an aqueous one containg an alkali metal hydroxide whereas the second comprises bromoethyltri­bromobenzene in an alcohol as solvent.

Abstract translation: 本发明涉及一种生产结晶三溴苯乙烯的改进方法。 该方法涉及在相转移催化剂的帮助下在两相体系中除去溴化氢。 使用的一个相是含有碱金属氢氧化物的水溶液，而第二个包括在乙醇中作为溶剂的溴乙基二溴苯。

公开(公告)号：EP0217102A1

公开(公告)日：1987-04-08

申请号：EP86111585.5

申请日：1986-08-21

Applicant: YEDA RESEARCH AND DEVELOPMENT COMPANY, LTD.

Inventor： Revel, Michel ,  Chebath, JudithC/O Dept. of Virology

IPC: C12N15/52 ,  C12N9/00 ,  C07K7/10 ,  C12Q1/68 ,  G01N33/573

CPC classification number: C12N9/93 ,  C12Q1/6897 ,  G01N33/573 ,  Y10S435/81 ,  Y10S436/80 ,  Y10S436/804 ,  Y10S436/813 ,  Y10S530/806

Abstract: The present invention relates to a human DNA sequence coding for an enzyme having (2'-5') oligo A synthetase activity, a 1.6 kb RNA and a 1.8 kb RNA being complementary to the mentioned DNA sequences, to a DNA transfer vector, comprising an inserted DNA sequence consisting essentially of the mentioned DNA sequence, furthermore to a microorganism which has been transformed by the said transfer vector and is capable of expressing an enzyme having the (2'-5') oligo A synthetase activity. The present invention furthermore provides a method of monitoring the response of a patient to an interferon, which comprises measuring the concentration of (2'-5') oligo A synthetase mRNA in cells or body fluids of the patient by hybridizing the mRNA to DNA complementary thereto. In addition there are provided antigenic peptides and antibodies raised against the antigenic peptides which recognises and immunoprecipitates (2'-5') oligo A synthetase.

Abstract translation: 本发明涉及编码具有（2分钟-5分钟）寡聚A合成酶活性的酶的DNA DNA序列，与所述DNA序列互补的1.6kb RNA和1.8kb RNA的DNA转移载体，其包含 基本上由所述DNA序列组成的插入DNA序列，此外还与已经通过所述转移载体转化并能够表达具有（2分钟-5分钟）寡聚A合成酶活性的酶的微生物。 本发明还提供了一种监测患者对干扰素的反应的方法，其包括通过将mRNA与DNA互补来测量患者细胞或体液中（2分钟-5分钟）寡聚A合成酶mRNA的浓度 于此。 此外，还提供抗原肽和针对抗原肽产生的抗体，其识别和免疫沉淀（2分钟-5分钟）寡聚A合成酶。

公开(公告)号：EP0167834A2

公开(公告)日：1986-01-15

申请号：EP85106996.3

申请日：1985-06-05

Applicant: YEDA RESEARCH AND DEVELOPMENT COMPANY, LTD.

Inventor： Margel, Shlomo

IPC: C08F8/42 ,  C08G12/30 ,  G01N33/53

CPC classification number: G01N33/553 ,  C08F8/42 ,  C08G6/00 ,  C08L5/12 ,  G01N33/534 ,  C08F16/34

Abstract: A metal-containing polyaledehyde microsphere composed of a polyaldehyde microsphere to which a transition metal, e.g. Au, Ag, Pt, Pd, Tc, Fe, Ni or Co, is bound may be obtained in accordance with this invention. The polyaldehyde, e.g. polyacrolein or polyglutaraldehyde, may be encapsulated in agarose, and the mocrosphere may be radioactive or magnetic. The microphere may additionally have a compound having at least one amine group, e.g. a drug, antibody, antigen, enzyme or other protein, bound to its surface.
In one embodiment a transition metal is bound to a polyaldehyde microsphere by contacting the polyaldehyde microsphere with a suitable amount of an appropriate salt or acid of the transition metal under suitable conditions so as to cause the salt or acid to be reduced to a lower valence state and to bind to the microsphere. Some salts or acids may thus be reduced to the elemental state; others are further reduced with an appropriate reducing agent.
In another embodiment, a transition metal in elemental form is bound to a polyaldehyde microsphere by contacting the polyaldehyde microsphere with a suitable amount of a compound capable of complexing with a salt or acid of the transition metal under suitable conditions permitting binding of the compound to the microsphere, contacting the compound with an appropriate amount of an appropriate salt or acid of a transition metal under appropriate conditions permitting the compound to bind to the salt or acid, and reducing the salt or acid to the corresponding elemental metal by contacting it under effective reducing conditions and for a sufficient period of time with a sufficient amount of an effective reducing agent. The metal-containing microsphere of this invention is useful for such applications as cell labeling, cell separation, diagnostic methods, catalysis and coating methods.

Abstract translation: 由聚醛微球组成的含金属聚醛微球，过渡金属例如 Au，Ag，Pt，Pd，Tc，Fe，Ni或Co可以根据本发明获得。 聚醛，例如 聚丙烯醛或聚戊二醛可以包封在琼脂糖中，并且大球可以是放射性的或磁性的。 微球可另外具有含至少一个胺基的化合物， 与其表面结合的药物，抗体，抗原，酶或其他蛋白质。 在一个实施方案中，过渡金属通过在合适的条件下使聚醛微球与合适量的过渡金属的合适盐或酸接触以使盐或酸被还原至较低价态来结合至聚醛微球 并绑定到微球。 一些盐或酸因此可以被还原为元素状态; 其他用适当的还原剂进一步还原。 在另一个实施方案中，元素形式的过渡金属通过使聚醛微球与合适量的能够与过渡金属的盐或酸配位的化合物在适合的条件下接触而与聚醛微球结合，所述条件允许化合物与 在合适的条件下使化合物与适量的合适的过渡金属盐或酸接触，使化合物与盐或酸结合，并且通过使盐或酸在有效还原下接触将其还原为相应的元素金属 条件和足量的有效还原剂足够的时间。 本发明的含金属微球可用于细胞标记，细胞分离，诊断方法，催化和涂布方法等应用。

公开(公告)号：EP0123200A3

公开(公告)日：1985-11-06

申请号：EP84103985

申请日：1984-04-10

Applicant: YEDA RESEARCH AND DEVELOPMENT COMPANY, LTD.

Inventor： Bendel, Peter

IPC: G01N24/08

CPC classification number: G01R33/482 ,  G01R33/5617 ,  G01R33/56563

Abstract: NMR imaging apparatus comprising apparatus for applying a linear magnetic field gradientto a sample, apparatus for varying the magnetic field gradient in a predetermined manner, apparatus for obtaining an NMR signal based on the linear magnetic field gradient, apparatus for storing the NMR signal, apparatus for reconstructing an NMR image from the stored NMR signal, and display apparatus for displaying the reconstructed NMR image, and wherein the apparatus for obtaining an NMR signal includes apparatus for obtaining the NMR signal at times when cancellation of the signal component dependent on magnetic field inhomogeneities occurs. There is also provided a technique for NMR imaging using the above apparatus.

公开(公告)号：EP0126245A3

公开(公告)日：1985-09-18

申请号：EP84103503

申请日：1984-03-29

Applicant: YEDA RESEARCH AND DEVELOPMENT COMPANY, LTD.

Inventor： Fraenkel, Dan ,  Levy, Moshe ,  Cherniavsky, Margaret

IPC: B01J29/28 ,  C07C02/86 ,  C07C02/66

CPC classification number: C07C2/66 ,  B01J29/86 ,  C07C2/864 ,  C07C2529/86 ,  Y10S423/22 ,  C07C15/00

Abstract: There are provided crystallized borosilicate glass zeolite catalysts which are para-selective in the alkylation of benzene and derivatives thereof and beta-selective in the alkylation of naphthalene and derivatives thereof. There is provided a process for the production of such catalysts wherein a borosilicate type glass is reacted with a zeolite synthesis template. There is also provided a selective alkylation process for the alkylation of benzene and its derivatives and of naphthalene and its derivatives wherein these are reacted with an alkylation agent in the presence of such catalyst.

公开(公告)号：EP0106281A3

公开(公告)日：1985-01-09

申请号：EP83110011

申请日：1983-10-06

Applicant: YEDA RESEARCH AND DEVELOPMENT COMPANY, LTD. ,  Research Foundation for Mental Hygiene, Inc.

Inventor： Tamir, Hadassah ,  Karpiak, Stephen E. ,  Wilchek, Meir

IPC: C07D209/20 ,  C07C103/52 ,  A61K31/405 ,  A61K37/02

CPC classification number: C07K5/06156 ,  C07D209/20

Abstract: Analgesia-effecting 5-hydroxytryptophan derivatives are disclosed of the formulae:
and
wherein R is selected from the group consisting of C 2 -C 16 alkyl, C 3 -C 8 cycloalkyl, C 2 -C 16 alkenyl. C 2 -C 16 alkynyl, aryl containing up to 14 carbon atoms, and arylalkyl containing up to 20 carbon atoms;
R' and R" are each H or methyl; R 2 and R 2a are each H or R, as defined above; R 3 and R 3a are each H or alkyl containing from 1 to 4 carbons; R 4 and R 4a are each H or alkyl containing from 1 to 4 carbons; and their non-toxic, pharmacologically acceptable acid addition salts.

公开(公告)号：EP0105221A1

公开(公告)日：1984-04-11

申请号：EP83108837.2

申请日：1983-09-07

Applicant: YEDA RESEARCH AND DEVELOPMENT COMPANY, LTD.

Inventor： Revel, Michel ,  Wallach, David

IPC: G01N33/68 ,  G01N33/569

CPC classification number: C12Q1/70 ,  G01N33/56983 ,  G01N33/6866 ,  Y10S435/84 ,  Y10S435/975 ,  Y10S436/804 ,  Y10S436/808 ,  Y10S436/828

Abstract: There is provided an assay for the detection and quantitative determination of interferon. The assay is based on the exposure of certain cells to a solution containing the interferon which is to be determined, infecting the cells with a suitable virus, incubating for a predetermined period of time, lysing the infected cultures and determining the virus protein. The measurement of the virus proteins can be effected by ELISA or Radioimmunoassay. There is also provided a kitfor carrying out such assays.

公开(公告)号：EP0099133A2

公开(公告)日：1984-01-25

申请号：EP83106965.3

申请日：1983-07-15

Applicant: YEDA RESEARCH AND DEVELOPMENT COMPANY, LTD.

Inventor： Arnon, Ruth ,  Hurwitz, Esther ,  Kashi, Rena ,  Wilchek, Meir ,  Sela, Michael ,  Schechter, Bilha

IPC: A61K39/395 ,  C07K15/06

CPC classification number: A61K47/6883 ,  A61K47/6803

Abstract: @ There are provided anti-tumor agents comprising as active ingredient platinum complexed directly or via dextran-amine moieties to an anti-tumor immunoglobulin. These are prepared by reacting an anti-tumor immunoglobulin or such immunoglobulin coupled to a dextran-amine with a platinum salt. Immunoglobulins having a specific binding activity towards certain types of cancer cells can be used. Immunoglobulins specific for ovarian or testicular carcinoma or for renal adenocarcinoma complexed directly or via dextran-amine moieties with platinum are effective agents for the treatment of these malignancies.

Abstract translation: 提供了包含作为活性成分的铂直接或通过葡聚糖胺部分与抗肿瘤免疫球蛋白复合的抗肿瘤剂。 这些通过使抗肿瘤免疫球蛋白或与葡聚糖胺偶联的免疫球蛋白与铂盐反应来制备。 可以使用对某些类型的癌细胞具有特异性结合活性的免疫球蛋白。 对卵巢或睾丸癌或直接或通过葡聚糖部分与铂复合的肾腺癌特异的免疫球蛋白是治疗这些恶性肿瘤的有效药剂。

公开(公告)号：EP0087786A1

公开(公告)日：1983-09-07

申请号：EP83101883.3

申请日：1983-02-25

Applicant: YEDA RESEARCH AND DEVELOPMENT COMPANY, LTD.

Inventor： Margel, Shlomo

IPC: C08L5/12 ,  C08J3/12 ,  C12N11/08 ,  B01J47/00

CPC classification number: C08L5/12 ,  C08J3/12 ,  Y10S428/90 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2998

Abstract: There are provided magnetic and non-magnetic agarose (or agar) - polyaldehyde beads with diameters ranging from 40 microns up to 1 cm, and processes for the synthesis of such beads. The polyaldehyde compounds, e.g. polyacrolein, polymethacrolein or polyglutaraldehyde, were ued as microspheres or as powders. The agarosepolyaldehyde beads are capable of covalently binding in a single step, through their aldehyde groups, compounds containing primary amino groups or thiol groups, such as proteins, antibodies, enzymes and drugs. The beads are useful for various biological applications, e.g. affinity chromatography, hemoperfusion, ion exchange resins, cell labeling, diagnostic purposes and cell separation.