-
公开(公告)号：KR1020120073730A
公开(公告)日：2012-07-05
申请号：KR1020100135575
申请日：2010-12-27
Applicant: 한국화학연구원
CPC classification number: C08H8/00 , C08B37/00 , C08J2397/02 , C12P19/04
Abstract: PURPOSE: An extraction method of low molecular water-soluble dietary fiber from lignocelluloses-based biomass is provided to extract low molecular water-soluble dietary fiber which includes more than disaccharides in high yield. CONSTITUTION: An extraction method of low molecular water-soluble dietary fiber from lignocelluloses-based biomass comprises the following steps: hot water extracting lignocelluloses-based biomass at 140-170 deg. Celsius for 10 minutes to 5 hours by suspending the lignocelluloses-based biomass in extract solvent in order for pH of extract to be 3.8-4.5 after extraction of water-soluble dietary fiber; and rapidly cooling the hot water extract to room temperature. The extraction method additionally includes a step of adding base in order for pH of extract to be 3.8-4.5 before suspension of the biomass in the extraction solvent.
Abstract translation: 目的：提取以木质纤维素为基础的生物质提取低分子水溶性膳食纤维的提取方法，以提取低分子量水溶性膳食纤维，其含量高于二糖。 构成：基于木质纤维素的生物质的低分子水溶性膳食纤维的提取方法包括以下步骤：在140-170度的热水提取基于木质纤维素的生物质。 摄取10分钟至5小时，将木质纤维素基生物质悬浮在萃取溶剂中，以提取水溶性膳食纤维后提取物的pH值为3.8-4.5; 并迅速将热水提取物冷却至室温。 提取方法还包括在将提取物的pH提取物悬浮在萃取溶剂中之前，将碱的添加量提高至3.8-4.5的步骤。
-
公开(公告)号：KR1020120073087A
公开(公告)日：2012-07-04
申请号：KR1020110113201
申请日：2011-11-02
Applicant: 한국화학연구원
CPC classification number: C12P19/02 , C12P19/14 , C12P2201/00 , C13K1/02
Abstract: PURPOSE: A biomass processing method and an additive used for the method are provided to effectively absorb lignin-derived material and various activity inhibition materials, thereby promoting saccharification of cellulose by cellulose hydrolysis. CONSTITUTION: A biomass processing method comprises the following steps: suspending the biomass in water or aqueous acid solution; processing enzyme saccharification after acid pre-treating or autohydrolysis the biomass; and adding an additive selected from natural silicate, artificial silicate, zirconia, heat resistant organic polymer, activated carbon, and a mixture thereof to enzyme saccharification or the pre-treating. The biomass is crushed material or powdered form. The heat resistant organic polymer is polytetrafluoroethylene.
Abstract translation: 目的：提供用于该方法的生物质处理方法和添加剂，以有效吸收木质素衍生的材料和各种活性抑制物质，从而通过纤维素水解促进纤维素的糖化。 构成：生物质处理方法包括以下步骤：将生物质悬浮于水或酸水溶液中; 经过酸处理或自动水解生物质后处理酶糖化; 并将选自天然硅酸盐，人造硅酸盐，氧化锆，耐热有机聚合物，活性炭及其混合物的添加剂添加到酶糖化或预处理中。 生物质是粉碎的材料或粉末形式。 耐热有机聚合物是聚四氟乙烯。
-
153.发明公开
公开(公告)号：KR1020120072562A
公开(公告)日：2012-07-04
申请号：KR1020100134368
申请日：2010-12-24
Applicant: 한국화학연구원
CPC classification number: C12P13/005 , C12N9/88 , C12Y401/01015
Abstract: PURPOSE: A method for preparing GABA from mono sodium glutamate using a Lactobacillus strain or recombinant Lactobacillus strain is provided to ensure high productivity. CONSTITUTION: A method for preparing GABA by Lactobacillus strain comprises: a step of culturing the strain in a medium containing MSG(mono sodium glutamate) and glycine, chloroform, or EDTA for 48-96 hours; and a step of collecting GABA from the culture liquid. The strain is Lactobacillus brevis.
Abstract translation: 目的：提供使用乳酸杆菌菌株或重组乳杆菌菌株从单一谷氨酸钠制备GABA的方法，以确保高生产率。 构成：通过乳杆菌菌株制备GABA的方法包括：在含有MSG（单谷氨酸钠）和甘氨酸，氯仿或EDTA的培养基中培养菌株48-96小时的步骤; 以及从培养液中收集GABA的步骤。 该菌株是短乳杆菌（Lactobacillus brevis）。
-
-
公开(公告)号：KR1020110083918A
公开(公告)日：2011-07-21
申请号：KR1020100003900
申请日：2010-01-15
Applicant: 한국화학연구원
Abstract: PURPOSE: A method for producing butnaol of high productivity using a strain is provided to be used in industrial technology. CONSTITUTION: A mutant Clostridium beijerinckii CBE-2-68 which produces butanol with high yield is deposited in the deposit number KCTC11606BP. A method for producing a large amount of butanol comprises: a step of culturing the Clostridium beijerinckii CBE-2-68 strain; a step of centrifuging the culture liquid to collect supernatant; and a step of purifying butanol.
Abstract translation: 目的：提供一种用于生产应用菌株的高生产率的方法，用于工业技术。 构成：以沉积物编号KCTC11606BP沉积产生高产率丁醇的突变型梭菌C.EBE-2-68。 一种生产大量丁醇的方法包括：培养梭菌Clothidium beijerinckii CBE-2-68菌株的步骤; 将培养液离心收集上清液的步骤; 和纯化丁醇的步骤。
-
Patent Agency Ranking
公开(公告)号：KR1020110068131A
公开(公告)日：2011-06-22
申请号：KR1020090124974
申请日：2009-12-15
Applicant: 한국화학연구원
Abstract: PURPOSE: A peroxidase formulation with improved catalyst activity and a method for preparing the same are provided to prepare a compound with sulfoxide group. CONSTITUTION: A peroxidase formulation with a heme active site contains: a hetero ring of imidazole, benzimidazole, thiazole, or isooxazole; hetero ring compounds; and buffer as an active ingredient. The hetero ring compound is imidazole, 2-methyl imidazole, 1,2-dimethyl imidazole, imidazole carboxylic acid, benzimidazole, or benzimidazole-5-carboxylic acid. The peroxidase formulation additionally contains a carrier. The carrier is crystalline cellulose.
Abstract translation: 目的：提供具有改善的催化剂活性的过氧化物酶制剂及其制备方法，以制备具有亚砜基团的化合物。 构成：具有血红素活性位点的过氧化物酶制剂含有：咪唑，苯并咪唑，噻唑或异恶唑的杂环; 杂环化合物; 和缓冲液作为活性成分。 杂环化合物是咪唑，2-甲基咪唑，1,2-二甲基咪唑，咪唑羧酸，苯并咪唑或苯并咪唑-5-羧酸。 过氧化物酶制剂另外含有载体。 载体是结晶纤维素。
-
公开(公告)号：KR1020100019127A
公开(公告)日：2010-02-18
申请号：KR1020080078005
申请日：2008-08-08
Applicant: 한국화학연구원
Abstract: PURPOSE: A method for preparing strain which has enhanced butanol tolerance and produced butanol with high yield is provided to usefully use in biobutanol commercialization. CONSTITUTION: A method for measuring butanol content in a sample comprises: a step of reacting reagent containing alcodhol dehydrogenase, reaction buffer, and co-enzyme NAD+; a step of measuring absorbance of reagent at 340nm; a step of performing the reaction using a sample which possibly contains butanol; a step of measuring absorbance of the sample; and a step of comparing standard curve and calculating butanol content.
Abstract translation: 目的：提供一种制备具有增强的丁醇耐受性并以高产率生产丁醇的菌株的方法，用于生物丁醇商业化。 构成：测量样品中丁醇含量的方法包括：使含有蜂蜡脱氢酶，反应缓冲液和辅酶NAD +的试剂反应的步骤; 测量试剂在340nm处的吸光度的步骤; 使用可能含有丁醇的样品进行反应的步骤; 测量样品的吸光度的步骤; 并比较标准曲线和计算丁醇含量的步骤。
-
公开(公告)号：KR1020090128767A
公开(公告)日：2009-12-16
申请号：KR1020080054696
申请日：2008-06-11
Applicant: 한국화학연구원
CPC classification number: C12P13/00 , B01J21/04 , B01J23/02 , B01J23/04 , B01J23/26 , B01J23/30 , C08G73/026 , C12N9/88 , C12N15/70 , C12N15/746 , C12Y401/01015
Abstract: PURPOSE: A novel method for preparing nylon 4 combining enzyme reaction and chemical reaction is provided to produce from biomass-derived raw material and reduce production cost. CONSTITUTION: A method for preparing nylon 4 from biomass through enzyme reaction and chemical reaction comprises: a first step of using glutamic acid or glutamic acid dicarboxylase(GAD) to obtain 4-aminobutylic acid; a second step of producing 2-pyrrolidone from 4-aminobutylic acid using catalyst or dehydrating agent; and a third step of adding initiator and catalyst from 2-pyrrolidone to obtain the nylon 4. The glutamic acid dicarboxylase is gadA or gadB enyme from E.coli or gad1, gad2 or gad3 enzyme from lactobacillus. The E.coli is Escherichia coli W3110. The catalyst is alumina(Al2O3), chromium oxide (Cr2O3), or titanium dioxide (TiO2) or tungsten oxide (WO3).
Abstract translation: 目的：提供生物质原料生产尼龙4组合酶反应和化学反应的新方法，降低生产成本。 构成：通过酶反应和化学反应从生物质制备尼龙4的方法包括：使用谷氨酸或谷氨酸二羧酸酶（GAD）获得4-氨基丁酸的第一步; 使用催化剂或脱水剂从4-氨基丁酸生产2-吡咯烷酮的第二步; 以及从2-吡咯烷酮中加入引发剂和催化剂以获得尼龙4的第三步骤。谷氨酸二羧酸酶是来自大肠杆菌的gadA或gadB enyme或来自乳杆菌的gad1，gad2或gad3酶。 大肠杆菌是大肠杆菌W3110。 催化剂是氧化铝（Al 2 O 3），氧化铬（Cr 2 O 3）或二氧化钛（TiO 2）或氧化钨（WO 3）。
-
公开(公告)号：KR100903008B1
公开(公告)日：2009-06-17
申请号：KR1020070115831
申请日：2007-11-14
Applicant: 한국화학연구원
IPC: C07C29/15 , C07C29/156 , C07C31/12
Abstract: 본 발명은, 구리계 촉매를 사용하여 물을 사용하지 않는 반응 조건 하에서, n-부탄산을 단독으로 직접 기상 수소화시키거나 또는 무수 n-부탄산이나 n-부탄산의 n-부틸에스테르와 같은 부탄산 유도체를 함유한 n-부탄산을 직접 기상 수소화하는 공정에 있어서 부반응의 억제 하에 높은 선택성 및 높은 공간 수율로 n-부탄올을 제조할 수 있는 수소화 방법을 제공하는 것이다. 구체적으로, 본 발명은, 환원된 구리계 촉매 상에서 n-부탄산을 수소에 의하여 직접 기상 환원시키는 것을 포함하는, n-부탄올의 제조 방법으로서, 상기 환원형 구리계 촉매가, 실리카, 알루미나, 티타니아 및 산화아연으로 이루어진 군에서 선택되는 하나 이상의 희석제와 산화구리 성분의 복합 산화물을 환원시켜 수득된 구리계 촉매이거나, 또는 상기 촉매에 코발트, 아연, 망간, 루테늄, 레늄, 팔라듐, 백금, 은, 텔루륨, 셀레륨, 마그네슘 및 칼슘으로 이루어진 군에서 선택되는 하나 이상의 개량성분을 추가로 포함한 촉매로서, 산화구리 성분의 함량이 40~95wt%이고 산화구리 입자크기가 50nm 이하가 되도록 제조된 촉매이다.
n-부탄산, n-부탄올, 바이오부탄올, 환원형 구리계 촉매-
公开(公告)号：KR100864011B1
公开(公告)日：2008-10-16
申请号：KR1020070062383
申请日：2007-06-25
Applicant: 한국화학연구원
Abstract: A method for preparing polypeptide libraries is provided to prepare a large quantity of polypeptide libraries within a short time through a simple manipulation, so that the polypeptide libraries are useful for screening new physiological or functional polypeptides. The polypeptide libraries are prepared by treating a parent protein with a protein-decomposing enzyme including endoproteinase selected from trypsin, chymotrypsin, pepsin, elastase, papain, thermolysin, endoproteinase Glu-C, endoproteinase Lys-C and endoproteinase Arg-C; a protein cleavage reagent selected from cyanogens bromide, formic acid, hydroxylamine and 2-(2'-nitrophenylsulfonyl-3-methyl-3-bromoindolenine); or a mixture thereof to prepare a mixture of polypeptides having lower number of amino acid than the parent protein, and optionally stabilizing the polypeptide mixture by reducing disulfide bonds and alkylating it.
Abstract translation: 提供制备多肽文库的方法，以通过简单的操作在短时间内制备大量的多肽文库，使得多肽文库可用于筛选新的生理或功能性多肽。 通过用蛋白质分解酶（包括选自胰蛋白酶，糜蛋白酶，胃蛋白酶，弹性蛋白酶，木瓜蛋白酶，嗜热菌蛋白酶，内蛋白酶Glu-C，内蛋白酶Lys-C和内蛋白酶Arg-C）的内蛋白酶处理亲本蛋白来制备多肽文库; 选自氰基溴，甲酸，羟胺和2-（2'-硝基苯基磺酰基-3-甲基-3-溴二氢化茚）的蛋白质裂解试剂; 或其混合物以制备具有比母体蛋白质氨基酸数低的多肽的混合物，以及任选地通过还原二硫键并将其烷基化来稳定多肽混合物。
-
-
-
-
-
-
-
-
-
Search Results
171
records
(took 0.034 seconds)
