公开(公告)号：WO1994005967A1

公开(公告)日：1994-03-17

申请号：PCT/US1993001421

申请日：1993-02-17

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  FUJITSU LABORATORIES LIMITED ,  SHIRASAKI, Masataka

Inventor： MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  FUJITSU LABORATORIES LIMITED

IPC: G01B09/02

CPC classification number: G01J9/02 ,  G01J2009/0226 ,  G02F1/3519 ,  G02F2001/211

Abstract: An interferometric measurement scheme utilizing squeezed light wherein an input pulse is split (14) into two consecutive input pulses (15a, 15b) separated by a time interval that is less than the inverse spectral width of GAWBS. The two pulses are further split (16) into first (20a, 20b) and second (22a, 22b) pairs of pulses and are caused to propagate in opposite directions through a fiber optic interferometer loop (18) and are recombined upon exiting the loop (16). The recombined pulses are caused to pass through a (pi) phase modulator (38) which modulates one of the two pulses. The output is then introduced to a balanced detector (50) where the detected signal of the two is averaged such that GAWBS noise is cancelled.

Abstract translation: 一种使用挤压光的干涉测量方案，其中输入脉冲被分离（14）成两个连续的输入脉冲（15a，15b），间隔时间间隔小于GAWBS的反谱带宽度。 两个脉冲进一步分裂成第一（20a，20b）和第二（22a，22b）的脉冲对，并通过光纤干涉仪回路（18）沿相反的方向传播，并且在离开环路 （16）。 使重新组合的脉冲通过调制两个脉冲之一的（pi）相位调制器（38）。 然后将输出引入平衡检测器（50），其中两者的检测信号被平均，使得GAWBS噪声被消除。

公开(公告)号：WO1993025685A1

公开(公告)日：1993-12-23

申请号：PCT/US1993005701

申请日：1993-06-14

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  HORVITZ, H., Robert ,  YUAN, Junying ,  SHAHAM, Shai

IPC: C12N15/12

CPC classification number: C12N9/6475 ,  A61K38/00 ,  C07K14/43581 ,  C07K14/4747

Abstract: Described herein are genes shown to be essential for programmed cell death in C. elegans, their encoded products (RNA and polypeptides), antibodies directed against the encoded polypeptides; probes for identifying structurally related genes and bioassays for identifying functionally related cell death genes from various organisms; methods and agents for altering (increasing or decreasing) the activity of the cell death genes and, thus, of altering cell death; and uses therefor. Specifically, two genes shown to be essential for almost all of the cell deaths which occur in the development of C. elegans, referred to as ced-3 and ced-4, have been cloned, sequenced and characterized.

Abstract translation: 本文描述的是显示为秀丽隐杆线虫程序性细胞死亡，其编码产物（RNA和多肽），针对编码多肽的抗体所必需的基因; 用于识别结构相关基因的探针和用于鉴定来自各种生物体的功能相关的细胞死亡基因的生物测定; 改变（增加或减少）细胞死亡基因的活性，从而改变细胞死亡的方法和试剂; 并用于此。 具体来说，已经克隆，测序和表征了显示对发生在秀丽隐杆线虫（称为ced-3和ced-4）中的几乎所有细胞死亡所必需的两个基因。

公开(公告)号：WO1993025247A1

公开(公告)日：1993-12-23

申请号：PCT/US1993005608

申请日：1993-06-11

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  MERRILL, Edward, W.

IPC: A61L33/00

CPC classification number: B01D67/0093 ,  A61K47/6903 ,  A61K47/6957 ,  A61L33/0029 ,  A61L33/0064 ,  A61L33/068 ,  B01D67/0088 ,  B01D2323/30 ,  B01D2323/34 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3225 ,  B01J20/3272 ,  B01J20/3282 ,  C07K1/22 ,  C08G65/329 ,  C08G65/332 ,  C08G65/333 ,  G01N33/545 ,  G02B1/043 ,  Y10S525/937 ,  C08L71/02

Abstract: This invention pertains to a method for immobilizing polyethylene oxide (PEO) star molecules in the form of hydrogel layers. The PEO star molecules are biocompatible and demonstrate non-thrombogenic properties. As such, the PEO star molecule layers have numerous biomedical applications, such as on contact lenses. The hydrogel layers contain a high percentage of terminal hydroxyl groups for attachment of affinity ligands and can be used for separating and purifying therapeutic proteins.

Abstract translation: 本发明涉及以水凝胶层的形式固定聚环氧乙烷（PEO）星形分子的方法。 PEO星形分子是生物相容性的并且表现出非血栓形成性质。 因此，PEO星形分子层具有许多生物医学应用，例如隐形眼镜。 水凝胶层含有高百分比的用于连接亲和配体的末端羟基，可用于分离和纯化治疗性蛋白质。

公开(公告)号：WO1993019705A1

公开(公告)日：1993-10-14

申请号：PCT/US1993002998

申请日：1993-03-29

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  ROLT, Kenneth, D. ,  LELE, Padmaker

IPC: A61F07/00

CPC classification number: G10K15/02 ,  A61B2017/22028 ,  A61N7/02 ,  A61N2007/006 ,  A61N2007/0073 ,  A61N2007/0078 ,  A61N2007/0095

Abstract: An ultrasound hyperthermia applicator suitable for medical hyperthermia treatment, and method for using the same, includes two ultrasound sources (14, 16) producing focused ultrasound beams of frequencies f0 and f1. Aiming means direct the two ultrasound beams (20, 22) so that they cross each other confocally at the target. A controller (11) activates the two ultrasound sources so that the target is simultaneously irradiated by the two focused ultrasound beams. The two ultrasound sources provide acoustic energy sufficient to cause significant intermodulation products to be produced at the target due to the interaction of the two ultrasound beams. The intermodulation products are absorbed by the target to enhance heating of the target. In preferred embodiments the ultrasound sources include a pair of signal generators (34, 36) for producing gated ultrasound output signals driving single crystal ultrasound transducers (14, 16). In other embodiments the ultrasound sources include a pair of phased array ultrasound transducers for generating two steerable ultrasound beams. Aiming means are provided for electronically steering and focusing the two ultrasound beams so that they cross each other confocally at the target. Further embodiments employ pluralities of transducers, arrays, or both.

Abstract translation: 适用于医疗高热治疗的超声热疗器及其使用方法包括产生频率为f0和f1的聚焦超声波束的两个超声波源（14,16）。 瞄准是指直接两个超声波束（20,22），使得它们在目标处共同交叉。 控制器（11）激活两个超声源，以使靶被同时被两个聚焦的超声波束照射。 由于两个超声波束的相互作用，两个超声波源提供了足够的声能，从而在目标上产生显着的互调产物。 互调产物被目标吸收，以增强目标的加热。 在优选实施例中，超声源包括一对用于产生驱动单晶超声换能器（14,16）的门控超声输出信号的信号发生器（34,36）。 在其他实施例中，超声源包括一对相控阵超声换能器，用于产生两个可操纵超声波束。 提供了用于电子地转向和聚焦两个超声波束的瞄准装置，使得它们在目标处共同交叉。 另外的实施例采用多个换能器，阵列或两者。

公开(公告)号：WO1993017132A1

公开(公告)日：1993-09-02

申请号：PCT/US1993001593

申请日：1993-02-24

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PAL, Uday ,  MURTHY, Gopala, Gazula, Krishna ,  HASHAM, Ali

IPC: C21C05/30

CPC classification number: C21C7/076 ,  C21C5/30 ,  Y02P10/242

Abstract: A process and apparatus for improving metal production in ironmaking and steelmaking processes is disclosed. The use of an inert metallic conductor in the slag-containing crucible and the addition of a transition metal oxide to the slag are the disclosed process improvements. The process comprises electric discharging of an electric charge build up in the slag.

公开(公告)号：WO1993017104A1

公开(公告)日：1993-09-02

申请号：PCT/US1993001545

申请日：1993-02-19

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  BROOK, J., David ,  HOUSMAN, David, E.

IPC: C12N15/12

CPC classification number: C12Q1/6883 ,  C07K14/4707 ,  C12N9/12 ,  C12Q2600/156

Abstract: A method by which a nucleotide sequence, specifically a CTG triplet repeat, shown to be expanded in individuals affected with DM can be identified in a sample obtained from an individual. The present method can be used to identify individuals in whom the CTG triplet repeat is present in normal copy number and individuals in whom the CTG triplet repeat occurs in abnormally high copy number, as well as to further identify individuals likely to be minimally affected and individuals likely to be more severely affected. The work described herein also makes available a DM transcription unit or gene which is likely to have an important role in the pathophysiology of DM.

Abstract translation: 在从个体获得的样品中可以鉴定出在受DM影响的个体中扩增的核苷酸序列，特别是CTG三联体重复序列的方法。 本方法可以用于鉴定CTG三联体重复存在于正常拷贝数中的个体和CTG三联体重复发生在异常高的拷贝数中的个体，以及进一步识别可能受到最小影响的个体和个体 可能会受到更严重的影响。 本文所述的工作还提供了可能在DM的病理生理学中具有重要作用的DM转录单元或基因。

公开(公告)号：WO1993015537A1

公开(公告)日：1993-08-05

申请号：PCT/US1993000838

申请日：1993-01-28

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  WALPOLE, James, N. ,  KINTZER, Emily, S. ,  CHINN, Stephen, R. ,  WANG, Christine, A. ,  MISSAGGIA, Leo, J.

IPC: H01S03/19

CPC classification number: H01S5/50 ,  H01S5/10 ,  H01S5/1017 ,  H01S5/1064 ,  H01S5/125

Abstract: A semiconductor laser gain structure having a tapered gain region (31) comprising cavity spoilers (48, 50) for receiving light which is reflected off of the output facet (38) back into the semiconductor and removing it from the gain region (31) so as to reduce or eliminate self-oscillation. The boundaries of the gain region (31) are also designed to have a very low refractive index gradient so as to minimize reflection of light off of the boundaries back into the gain region (31). The gain structure (33) may be embodied in a semiconductor laser oscillator or semiconductor laser amplifier depending on whether the input facet (34) is or is not, respectively, anti-reflection coated. The output facet (38) is anti-reflection coated in either embodiment.

公开(公告)号：WO1993009235A2

公开(公告)日：1993-05-13

申请号：PCT/US1992009562

申请日：1992-11-06

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  BAKER, Rohan, T. ,  TOBIAS, John, W. ,  VARSHAVSKY, Alexander

IPC: C12N15/57

CPC classification number: C12P21/06 ,  C07K14/00 ,  C07K2319/00 ,  C07K2319/95 ,  C12N9/60 ,  C12N15/62

Abstract: The disclosure relates to a generic class of ubiquitin-specific proteases which specifically cleave at the C-terminus of the ubiquitin moiety in a ubiquitin fusion protein irrespective of the size of the ubiquitin fusion protein. More specifically, the disclosure relates to ubiquitin-specific proteases of this class which have been isolated from a cell. The disclosure also relates to isolated DNA sequences encoding the proteases of this class.

Abstract translation: 本公开涉及泛素特异性蛋白酶的泛型，其泛素泛素融合蛋白中泛素部分的C末端特异性切割，而与泛素融合蛋白的大小无关。 更具体地，本公开涉及已经从细胞中分离的该类的泛素特异性蛋白酶。 本公开还涉及编码该类蛋白酶的分离的DNA序列。

公开(公告)号：WO1993009225A1

公开(公告)日：1993-05-13

申请号：PCT/US1992009325

申请日：1992-10-30

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  ARCHER, John, A., C. ,  FOLLETTIE, Maximillian, T. ,  SINSKEY, Anthony, J.

IPC: C12N09/04

CPC classification number: C12N9/0006 ,  C12N9/12 ,  C12N9/88 ,  C12N15/52 ,  C12N15/77 ,  C12P13/08

Abstract: Mutagenesis of the gene encoding homoserine dehydrogenase (hom) for production of the amino acid threonine is described. The mutation causes an alteration in the carboxy terminus of the enzyme that interferes with end-product inhibition by threonine. The lack of end-product inhibition causes an overproduction of threonine.

Abstract translation: 描述了用于产生氨基酸苏氨酸的编码高丝氨酸脱氢酶（hom）的基因的突变。 该突变导致酶的羧基末端的改变，其干扰苏氨酸的最终产物抑制。 最终产物抑制的缺乏导致苏氨酸的过量产生。

公开(公告)号：WO1993008289A1

公开(公告)日：1993-04-29

申请号：PCT/US1992009124

申请日：1992-10-22

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  SASISEKHARAN, Ramnath ,  MOREMEN, Kelley ,  COONEY, Charles, L. ,  ZIMMERMANN, Joseph, J. ,  LANGER, Robert, S.

IPC: C12N15/60

CPC classification number: C12N9/88

Abstract: The cloning of the heparinase gene from Flavobacterium Heparinum using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino acid sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. Coli.

Abstract translation: 描述了使用聚合酶链反应从肝炎杆菌中克隆肝素酶基因。 开放阅读框（ORF）对应于编码MW 43,800道尔顿的前体蛋白质的1152个碱基对。 氨基酸序列显示20个残基的前导肽。 该基因在大肠杆菌的两个表达系统中表达。