Light-switchable gene expression system and the methods for controlling gene expression in prokaryotic bacterium

    公开(公告)号:US10184126B2

    公开(公告)日:2019-01-22

    申请号:US14419106

    申请日:2013-08-01

    Abstract: Provided is an optically controlled gene expression system of prokaryotic bacterium, comprising: a) a photosensitive recombinant transcription factor encoding gene, the photosensitive recombinant transcription factor is one fusion protein comprising a first polypeptide as the DNA bonding domain and a second polypeptide as the photosensitive domain; b) a target transcription unit comprising promoter or promoter-reaction element or reaction element-promoter containing at least one reaction element recognized/bound by the first polypeptide and the nucleic acid sequence to be transcribed. Also provided is a prokaryotic expression vector comprising said optically controlled gene expression system, and a method for regulating gene expression in a prokaryotic host cell by using the optically controlled gene expression system. Also provided is a reagent kit containing different components of the optically controlled gene expression system. The optically controlled gene expression system of prokaryotic bacterium has a quick, effective and powerful induction, is safer than other inducers, is of little or no toxicity, and can control gene expression both spatially and temporally, and can regulate many life processes of prokaryotic bacterium.

    CATALYSTS, METHODS, AND SYSTEMS FOR PREPARING CARBAMATES
    170.
    发明申请
    CATALYSTS, METHODS, AND SYSTEMS FOR PREPARING CARBAMATES 有权
    催化剂,制备碳酸盐的方法和系统

    公开(公告)号:US20170015622A1

    公开(公告)日:2017-01-19

    申请号:US15124397

    申请日:2014-03-13

    Abstract: Methods, systems and kits for preparing carbamates as well as catalysts for preparing the carbamates, are disclosed. The methods for preparing carbamate can include providing a catalyst comprising cerium oxide (CeO2) and at least one metal selected from the group consisting of iron (Fe), manganese (Mn), titanium (Ti), cobalt (Co), aluminum (Al), zinc (Zn), calcium (Ca), tin (Sn), indium (In), and any combination thereof; contacting the catalyst with at least one amine and at least one alcohol to form a mixture; and contacting the mixture with carbon dioxide under conditions sufficient to form the carbamate.

    Abstract translation: 公开了用于制备氨基甲酸酯的方法,系统和试剂盒以及用于制备氨基甲酸酯的催化剂。 制备氨基甲酸酯的方法可以包括提供包含氧化铈(CeO 2)和至少一种选自铁(Fe),锰(Mn),钛(Ti),钴(Co),铝(Al ),锌(Zn),钙(Ca),锡(Sn),铟(In)及其任何组合; 使催化剂与至少一种胺和至少一种醇接触以形成混合物; 并在足以形成氨基甲酸酯的条件下将混合物与二氧化碳接触。

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