公开(公告)号：WO2010003059A2

公开(公告)日：2010-01-07

申请号：PCT/US2009/049517

申请日：2009-07-02

Applicant: LIFE TECHNOLOGIES CORPORATION ,  BARTEL, Joseph ,  CHEN, Yongfen ,  TULSKY, Eric ,  TREADWAY, Joseph

Inventor： BARTEL, Joseph ,  CHEN, Yongfen ,  TULSKY, Eric ,  TREADWAY, Joseph

IPC: B82B1/00 ,  B82B3/00

CPC classification number: G01N21/6486 ,  B82Y5/00 ,  B82Y15/00 ,  C01B25/082 ,  C09K11/02 ,  C09K11/565 ,  C09K11/70 ,  C30B7/00 ,  C30B29/40 ,  C30B29/48 ,  C30B29/60 ,  G01N33/588 ,  H01L21/02392 ,  H01L21/02557 ,  H01L21/02601 ,  H01L21/02628 ,  H01L33/06

Abstract: Nanocrystals having an indium-based core and methods for making them and using them to construct core-shell nanocrystals are described. These core-shell nanocrystals are highly stable and provide higher quantum yields than known nanocrystals of similar composition, and they provide special advantages for certain applications because of their small size.

Abstract translation: 描述了具有铟基核的纳米晶体及其制造方法以及使用它们构建核 - 壳纳米晶体的方法。 这些核 - 壳纳米晶体非常稳定并且提供比具有类似组成的已知纳米晶体更高的量子产率，并且由于它们的小尺寸，它们为某些应用提供了特别的优势。

公开(公告)号：WO2009155373A1

公开(公告)日：2009-12-23

申请号：PCT/US2009/047705

申请日：2009-06-17

Applicant: LIFE TECHNOLOGIES CORPORATION ,  CHEN, Linda ,  BRODEY, Mary ,  STOLLAR, Neil ,  BEERNINK, Hans ,  REAGAN, Kevin

Inventor： CHEN, Linda ,  BRODEY, Mary ,  STOLLAR, Neil ,  BEERNINK, Hans ,  REAGAN, Kevin

IPC: C12N15/12 ,  C07K14/54 ,  C07K16/24 ,  G01N33/53

CPC classification number: C07K14/5412

Abstract: The present invention provides the amino acid sequence for guinea pig IL-6, a biologically active recombinant guinea pig IL-6 protein, as well as the DNA sequence encoding the IL-6 protein. Also provided herein are novel biomarkers, antibodies and polypeptides related to guinea pig IL-6 protein. Also provided are detection panels for detecting biomarkers related to guinea pig IL-6 protein and methods for detection or diagnosis of inflammatory conditions such as chronic obstructive pulmonary disease.

Abstract translation: 本发明提供了豚鼠IL-6，生物活性重组豚鼠IL-6蛋白以及编码IL-6蛋白的DNA序列的氨基酸序列。 本文还提供了与豚鼠IL-6蛋白相关的新型生物标志物，抗体和多肽。 还提供了用于检测与豚鼠IL-6蛋白相关的生物标志物的检测板和用于检测或诊断诸如慢性阻塞性肺疾病的炎性病症的方法。

公开(公告)号：WO2009131805A1

公开(公告)日：2009-10-29

申请号：PCT/US2009/039015

申请日：2009-03-31

Applicant: LIFE TECHNOLOGIES CORPORATION ,  ROSENBLUM, Barnett, G. ,  MENCHEN, Steven, M.

Inventor： ROSENBLUM, Barnett, G. ,  MENCHEN, Steven, M.

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q2535/101 ,  C12Q2525/186 ,  C12Q2521/101

Abstract: Disclosed are methods and kits applicable to sequencing methods, such as Sanger dideoxy sequencing methods. The methods and kits disclosed utilize a cationically charged nucleic acid terminator in combination with a discriminatory polymerase.

Abstract translation: 公开了适用于测序方法的方法和试剂盒，例如Sanger双脱氧测序方法。 所公开的方法和试剂盒与阳离子电荷的核酸终止子结合歧视聚合酶。

公开(公告)号：WO2009129327A1

公开(公告)日：2009-10-22

申请号：PCT/US2009/040698

申请日：2009-04-15

Applicant: LIFE TECHNOLOGIES CORPORATION ,  CHANG, Jun-Keun ,  CHO, Keun-Chang ,  CHUNG, Chan-Il ,  JUNG, Neon-Cheol ,  HUH, Seung, Gin

Inventor： CHANG, Jun-Keun ,  CHO, Keun-Chang ,  CHUNG, Chan-Il ,  JUNG, Neon-Cheol ,  HUH, Seung, Gin

IPC: C12M3/00 ,  C12M1/42

CPC classification number: C12M35/02

Abstract: Provided herein is a pipette tip (600) for electroporation including an outer surface, a void (611) located within the outer surface, and a conductor (622) located at least partially on or within the outer surface, in electrical communication with at least a portion of the outer surface and the void. Further provided herein is a pipette tip for electroporation including a body, a connector (620), and an elongated part (630). The connector is located at the distal end of the body and further includes at least one connecting post, a connecting part in mechanical communication with the connector, and a conductor located at least partially on or within the connector, wherein the conductor surrounds at least a portion of the connecting post. The elongated part also has a void and is located at the distal end of the connector. The void of the body, connector, and elongated part are in fluid communication.

Abstract translation: 本文提供了一种用于电穿孔的移液管尖端（600），其包括外表面，位于外表面内的空隙（611）和至少部分地位于外表面上或外表面上的导体（622），至少与 外表面和空隙的一部分。 本文进一步提供用于电穿孔的移液管末端，其包括主体，连接器（620）和细长部分（630）。 连接器位于主体的远端，并且还包括至少一个连接柱，与连接器机械连通的连接部分和至少部分地位于连接器上或内部的导体，其中导体至少围绕 连接柱的一部分。 细长部分还具有空隙并且位于连接器的远端。 主体，连接器和细长部分的空隙处于流体连通状态。

公开(公告)号：WO2009121021A1

公开(公告)日：2009-10-01

申请号：PCT/US2009/038669

申请日：2009-03-27

Applicant: LIFE TECHNOLOGIES CORPORATION ,  WELCH, Peter

Inventor： WELCH, Peter

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q2525/197 ,  C12Q2521/531

Abstract: Disclosed are arrays treatable to prevent reuse. The disclosed arrays include probes that contain nucleotide analogs susceptible to a degradative agent. After analysis, such arrays can be treated with the degradative agent, thereby rendering them unsuitable for reuse. Thus, the present disclosure describes compositions and methods for degrading array probes, thereby preventing array reuse.

Abstract translation: 公开了可处理以防止重复使用的阵列。 所公开的阵列包括含有对降解剂敏感的核苷酸类似物的探针。 分析后，可以用降解剂处理这样的阵列，从而使它们不适合再利用。 因此，本公开描述了用于降解阵列探针的组合物和方法，从而防止阵列再利用。

公开(公告)号：WO2009120883A3

公开(公告)日：2009-10-01

申请号：PCT/US2009/038431

申请日：2009-03-26

Applicant: LIFE TECHNOLOGIES CORPORATION ,  BENNETT, Robert

Inventor： BENNETT, Robert

IPC: C12N15/11 ,  C12N7/04

Abstract: The invention provides compositions and methods for delivering compounds to cells. The invention is directed, in part, to virus-like particles which contain biological materials such as carbohydrates, proteins and nucleic acids. The invention is also directed, in part, to methods for delivering compounds to cells involving contacting cells with the compounds under conditions which allow for uptake of the compounds by cells and release of the compounds into the cells which take it up.

公开(公告)号：WO2009108905A2

公开(公告)日：2009-09-03

申请号：PCT/US2009/035591

申请日：2009-02-27

Applicant: LIFE TECHNOLOGIES CORPORATION ,  PIPER, David ,  VOGEL, Kurt ,  SHEKHANI, Mohammed Saleh ,  HESS, Stephen ,  DUFF, Steve ,  LIVELLI, Thomas ,  ZHONG, Zhong

Inventor： PIPER, David ,  VOGEL, Kurt ,  SHEKHANI, Mohammed Saleh ,  HESS, Stephen ,  DUFF, Steve ,  LIVELLI, Thomas ,  ZHONG, Zhong

IPC: G01N33/58

CPC classification number: G01N33/582 ,  C07D491/107 ,  G01N33/6872 ,  Y10T436/13

Abstract: Disclosed are assays, methods, and kits for the screening of test compounds for their capability to induce cardiotoxicity in a subject. In particular, whether a test compound has the effect to prolong the Q-T interval as measured by an electrocardiogram in a human. The assays, methods, and kits disclosed herein make use of the binding interaction between novel fluorescent tracers and the hERG K+ channel, and the propensity of a test compound to influence that binding interaction.

Abstract translation: 公开了用于筛选测试化合物在受试者中诱导心脏毒性的能力的测定法，方法和试剂盒。 具体而言，测试化合物是否具有延长人类心电图所测量的Q-T间期的作用。 本文公开的测定法，方法和试剂盒利用新颖荧光示踪剂与hERG K +通道之间的结合相互作用以及测试化合物影响该结合相互作用的倾向。

公开(公告)号：WO2009103050A1

公开(公告)日：2009-08-20

申请号：PCT/US2009/034213

申请日：2009-02-16

Applicant: MDS ANALYTICAL TECHNOLOGIES ,  LIFE TECHNOLOGIES CORPORATION ,  THOMSON, Bruce A. ,  LE BLANC, Yves

Inventor： THOMSON, Bruce A. ,  LE BLANC, Yves

IPC: G01N31/00

CPC classification number: H01J49/0009

Abstract: Quantitation is performed using data from a mass spectrometer. A calibration ion mass spectrum is acquired for each of a plurality of known quantities of a material. From the calibration spectra a plurality of ions that identify the material is determined, and for each ion of the plurality of ions a linear range and linear function are determined. A sample ion mass spectrum is acquired for an unknown quantity of the material. A sample intensity is measured for each ion of the plurality of ions from the sample spectrum. After acquiring the sample spectrum, one or more ions are selected from the plurality of ions such that the sample intensity of each of the one or more ions is within a linear range of the ion. The unknown quantity is calculated from the sample intensities and linear functions of the one or more ions.

Abstract translation: 使用质谱仪的数据进行定量。 对于多种已知量的材料中的每一种获取校准离子质谱。 从校准光谱中确定识别材料的多个离子，并且对于多个离子的每个离子确定线性范围和线性函数。 对于未知量的材料获取样品离子质谱。 从样品光谱测量多个离子的每个离子的样品强度。 在获取样品光谱之后，从多个离子中选出一个或多个离子，使得一个或多个离子中的每一个的样品强度在离子的线性范围内。 从一个或多个离子的样品强度和线性函数计算未知量。

公开(公告)号：WO2009094783A1

公开(公告)日：2009-08-06

申请号：PCT/CA2009/000123

申请日：2009-01-30

Applicant: MDS ANALYTICAL TECHNOLOGIES, A BUSINESS UNIT OF MDS INC., DOING BUSINESS THROUGH ITS SCIEX DIVISION ,  LIFE TECHNOLOGIES CORPORATION, A DELAWARE CORPORATION ,  MDS INC. ,  CHERNUSHEVICH, Igor ,  LOBODA, Alexandre V.

Inventor： CHERNUSHEVICH, Igor ,  LOBODA, Alexandre V.

IPC: H01J49/10 ,  H01J49/40

CPC classification number: H01J49/0045 ,  H01J49/062

Abstract: In a tandem mass spectrometer using a collision cell for ion fragmentation, the upper limit of the collision energy required for collision induced dissociation (CID) can be extended without reaching or going beyond the upper electrical discharge limit of the system components. The present teachings describe a method of lifting the potential energy of ions to a predetermined level sufficient for CID fragmentation while satisfying a discharge free condition. The present teaching also describes a method of lifting the potential energy of the fragment ions after CID fragmentation so that the product ions have sufficient energy for mass analysis.

Abstract translation: 在使用用于离子破碎的碰撞池的串联质谱仪中，碰撞诱导解离（CID）所需的碰撞能量的上限可以延长而不达到或超过系统部件的上限放电极限。 本教导描述了一种在满足无放电条件的同时将离子的势能提升到足以进行CID碎裂的预定水平的方法。 本教导还描述了在CID碎裂之后提升碎片离子的势能的方法，使得产物离子具有足够的能量进行质量分析。

公开(公告)号：WO2009094757A1

公开(公告)日：2009-08-06

申请号：PCT/CA2009/000085

申请日：2009-01-26

Applicant: MDS ANALYTICAL TECHNOLOGIES, a business unit of MDS Inc., doing business through its Sciex divison ,  LIFE TECHNOLOGIES CORPORATION ,  COLLINGS, Bruce

Inventor： COLLINGS, Bruce

IPC: H01J49/10 ,  H01J49/26

CPC classification number: H01J49/0481 ,  H01J49/422

Abstract: Methods for cooling ions retained in an ion trap are described. In various embodiments, a cooling gas is delivered into a linear ion trap causing a non-steady state pressure elevation in at least a portion of the trap above about 8 x 10 -5 Torr for a duration less than the ion-retention time. In various embodiments, the duration of pressure elevation can be based upon a period of time required for an ion to lose a desired amount of its kinetic energy.

Abstract translation: 描述了保留在离子阱中的离子的冷却方法。 在各种实施方案中，将冷却气体输送到线性离子阱中，导致捕集阱的至少一部分中的非稳态压力升高高于约8×10 -5乇，持续时间小于离子保留时间。 在各种实施方案中，压力升高的持续时间可以基于离子失去所需量的其动能所需的时间段。