公开(公告)号：WO1997010331A1

公开(公告)日：1997-03-20

申请号：PCT/US1996013708

申请日：1996-08-28

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  GONZALES, Diana

IPC: C12N15/10

CPC classification number: C12N15/1006 ,  Y10S435/803

Abstract: The invention discloses DNA isolation and purification methods which involve novel washing steps. The disclosed methods provide a means for isolating and purifying DNA from a homogeneous mixture of DNA of other cellular contaminants by treating silica with the homogeneous mixture containing DNA in the presence of a chaotropic salt solution and then washing and separating the washed and treated silica in successive wash steps with aqueous alcohol wash solutions. A first wash step involves washing the treated silica with a first wash solution of at least 95 wt.% alcohol in water. A second wash step similarly involves washing the treated and washed silica with second wash solution of less than 95 wt.% alcohol in water.

Abstract translation: 本发明公开了涉及新的洗涤步骤的DNA分离纯化方法。 所公开的方法提供了通过在离液盐溶液存在下用含有DNA的均匀混合物处理二氧化硅，然后在连续洗涤和分离洗涤和处理的二氧化硅的方法中从其他细胞污染物的DNA的均匀混合物中分离和纯化DNA的方法 用含水酒精洗涤液洗涤步骤。 第一洗涤步骤包括用至少95重量％的醇在水中的第一洗涤溶液洗涤经处理的二氧化硅。 第二洗涤步骤类似地涉及用小于95重量％的醇在水中的第二洗涤溶液洗涤经处理和洗涤的二氧化硅。

公开(公告)号：WO1997003352A1

公开(公告)日：1997-01-30

申请号：PCT/US1996011484

申请日：1996-07-10

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  PENTONEY, Stephen, L., Jr. ,  KONRAD, Kenneth, D.

IPC: G01N27/447

CPC classification number: G01N27/4473 ,  G01N27/44743

Abstract: An electrophoretic system employing a capillary with at least one end having an electrically conductive coating or layer directly thereon. In order to introduce a plug of sample from a small amount of the sample in a vial, the conductive tip is submersed into the small amount of sample and an electric field is applied at the end by applying the high voltage to the tip in order to electrokinetically inject a plug of the sample. The conductive tip may also be used as a terminal for completing the circuit for applying the high voltage across the capillary column for electrophoresis. The separated components may be collected on a surface or small amount of buffer to reduce sample dilution or mixing of one sample component with a different sample component from the electrophoretic process.

Abstract translation: 一种采用毛细管的电泳系统，其至少一端具有直接在其上的导电涂层或层。 为了在样品瓶中引入少量样品的样品塞，导电尖端浸入少量样品中，并通过向尖端施加高电压而在末端施加电场，以便 电动注射样品的塞子。 导电尖端还可以用作终端，用于完成用于在毛细管柱上施加高电压用于电泳的电路。 分离的组分可以在表面或少量缓冲液上收集，以减少样品稀释或一个样品组分与不同于电泳过程的不同样品组分的混合。

公开(公告)号：WO1996034278A1

公开(公告)日：1996-10-31

申请号：PCT/US1996005903

申请日：1996-04-26

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  PENTONEY, Stephen, L., Jr. ,  RAKESTRAW, David, J.

IPC: G01N27/447

CPC classification number: G01N27/44721

Abstract: A detection system for sequentially and repetitively scanning a plurality of sample volumes and detecting electromagnetic radiation emitting from each of the sample volumes is described. The system includes a plurality of coplanar side-by-side capillaries each containing a sample volume, an electromagnetic radiation source, a mirror aligned to receive and reflect electromagnetic radiation, a means for moving the mirror, and a detector aligned to receive electromagnetic radiation collected from the sample volumes. In operation, the means for moving the mirror adjusts the mirror position so that the mirror is positioned to receive the electromagnetic radiation from the electromagnetic radiation source and reflect the electromagnetic radiation sequentially and repetitively to the sample volumes. Emitted electromagnetic radiation from the sample volume is collected and directed to a detector where a signal is generated in response to the interaction of the electromagnetic radiation with the sample.

Abstract translation: 描述了用于顺次和重复地扫描多个采样体积并检测从每个样本体积发射的电磁辐射的检测系统。 该系统包括多个共面的并排毛细管，每个毛细管均包含样品体积，电磁辐射源，对准以接收和反射电磁辐射的反射镜，用于移动反射镜的装置和被对准以接收收集的电磁辐射的检测器 从样品量。 在操作中，用于移动反射镜的装置调节反射镜位置，使得反射镜被定位成接收来自电磁辐射源的电磁辐射，并且将电磁辐射依次反复地反射到样品体积。 收集来自样品体积的发射的电磁辐射并将其引导到检测器，其中响应于电磁辐射与样品的相互作用产生信号。

公开(公告)号：WO1996023219A1

公开(公告)日：1996-08-01

申请号：PCT/US1996000685

申请日：1996-01-22

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  KATZMANN, Steven, P. ,  GLENDAY, Ronald, C.

IPC: G01N27/447

CPC classification number: B01L3/508 ,  B01L2200/16 ,  G01N27/44704 ,  G01N27/44782 ,  G01N35/1002 ,  Y10T436/11

Abstract: A reagent segment (10) including a body and trough (30) formed therein with the floor of the trough sloped towards a drainage zone (40). The reagent segment is adapted for transporting reagents within an automated analyzer by having at least two protrusions (55A, 55B) on the exterior wall of the trough that fit within corresponding cavities of a transport arm. The reagent segment is retained by a latching mechanism through a protuberance (52) on the exterior surface of the trough that conforms with a hole in the latching mechanism. The reagent segment can further comprise a concave depression (42) on the interior surface of the trough at the drainage zone. The depression (42) can coincide with the protuberance (52) on the exterior surface of the trough that associates with the hole in the latching mechanism. Further, the reagent segment may include at least one indexing rib (62A, 62B), between the exterior surface of the trough and the rim of the body, that can be inserted within an indexing slot of a holding means. Thus, the reagent segment is uniquely adapted for automated handling and processing of reagents in an automated analyzer.

Abstract translation: 一种试剂部分（10），包括形成在其中的主体和槽（30），其中所述槽的底部朝向排水区（40）倾斜。 试剂段适于在自动化分析仪内运送试剂，在槽的外壁上具有至少两个突出部分（55A，55B），该突出部分配合在运输臂的相应空腔内。 试剂段通过闩锁机构通过槽的外表面上的凸起（52）保持，该凸起符合锁定机构中的孔。 试剂段可进一步包括在排水区的槽的内表面上的凹陷（42）。 凹陷（42）可以与槽的外表面上与闩锁机构中的孔相关联的突起（52）重合。 此外，试剂段可以包括在槽的外表面和主体的边缘之间的至少一个分度肋（62A，62B），其可插入保持装置的分度槽内。 因此，试剂段独特地适用于在自动化分析仪中自动处理和处理试剂。

公开(公告)号：WO1995020161A1

公开(公告)日：1995-07-27

申请号：PCT/US1995000805

申请日：1995-01-20

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  CHEN, Fu-Tai, A. ,  PENTONEY, Stephen, L.

IPC: G01N33/561

CPC classification number: C07H21/00 ,  C12Q1/40 ,  G01N33/561 ,  G01N2800/52 ,  Y10S436/80

Abstract: Homogeneous immunoassays and enzyme-substrate assays which use capillary electrophoresis and fluorescent detection systems are described. The method is useful for detecting and/or quantitating the concentration of analytes in a sample.

Abstract translation: 描述了使用毛细管电泳和荧光检测系统的均相免疫测定和酶底物测定法。 该方法可用于检测和/或定量样品中分析物的浓度。

公开(公告)号：WO1995010041A1

公开(公告)日：1995-04-13

申请号：PCT/US1994010402

申请日：1994-09-15

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  WANG, Hann-Ping ,  LIU, Cheng-Ming

IPC: G01N27/447

CPC classification number: G01N27/44726 ,  G01N27/44743

Abstract: A method of quantitating proteins in complex samples using capillary electrophoresis can be used both to determine the concentration of a protein in a sample and to determine total protein concentration in the sample. In general, the method of determining the concentration of a marker protein comprises: (1) adding a known quantity of an internal standard compound to a sample containing at least one protein, the internal standard compound selected from the group consisting of benzoic acid substituted with at least one halogen, producing a detector signal in relation to its concentration, and being capable of electrophoretic separation from the protein; (2) subjecting the sample and the internal standard compound to capillary electrophoresis to separate the protein and the internal standard compound from each other and from other components in the sample; (3) measuring the detector signal produced by the internal standard compound and a detector signal produced by the protein to determine a ratio of protein signal to internal standard signal; and (4) determining the concentration of the protein in the sample from a standard curve of protein concentration versus the ratio of protein signal to internal standard signal. A typical protein measurable by the method is albumin. Typically, measurements are made at 214 nm and the internal standard compound is 2,4-dichlorobenzoic acid.

Abstract translation: 使用毛细管电泳定量复杂样品中的蛋白质的方法可以用于确定样品中蛋白质的浓度并确定样品中的总蛋白质浓度。 通常，确定标记蛋白浓度的方法包括：（1）向含有至少一种蛋白质的样品中加入已知量的内标化合物，所述内标化合物选自由 至少一个卤素，产生与其浓度相关的检测器信号，并且能够与蛋白质电泳分离; （2）对样品和内标化合物进行毛细管电泳，将蛋白质和内标化合物与样品中的其他成分分离; （3）测量由内标化合物产生的检测器信号和由蛋白质产生的检测器信号，以确定蛋白质信号与内标信号的比率; 和（4）从蛋白质浓度的标准曲线与蛋白质信号与内标信号的比率确定样品中蛋白质的浓度。 通过该方法测量的典型蛋白质是白蛋白。 通常，测量在214nm，内标化合物是2,4-二氯苯甲酸。

公开(公告)号：WO1995002182A1

公开(公告)日：1995-01-19

申请号：PCT/US1994005631

申请日：1994-05-18

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  LIU, Cheng-Ming ,  WANG, Hann-Ping

IPC: G01N33/483

CPC classification number: G01N30/14 ,  G01N33/491 ,  G01N33/493 ,  Y10S436/828 ,  Y10T436/25125 ,  Y10T436/255

Abstract: Processes are provided for pretreating body fluid compositions and subsequently analyzing the pretreated body fluid compositions for analytes of interest. Processes for pretreating the compositions include providing size exclusion gel having a molecular weight fractionation range or a molecular weight exclusion such that the size exclusion gel is capable of excluding or fractionating the analytes of interest, and then causing the composition to contact the size exclusion gel in order to separate the analytes from low molecular weight composition components which interfere with the separation and analysis of the analytes of interest. Processes for analyzing pretreated compositions include electrophoretic methods such as capillary zone electrophoresis which involve the separation and detection of analytes of interest.

Abstract translation: 提供了用于预处理体液组合物并随后分析预处理的体液组合物用于所关注的分析物的方法。 用于预处理组合物的方法包括提供具有分子量分馏范围或分子量排除的尺寸排阻凝胶，使得尺寸排阻凝胶能够排除或分级分析所关注的分析物，然后使组合物与尺寸排阻凝胶接触 将分析物与低分子量组成成分分离开来，这些成分干扰了目的分析物的分离和分析。 用于分析预处理组合物的方法包括电泳方法，例如涉及分离和检测感兴趣分析物的毛细管区带电泳。

公开(公告)号：WO1994022584A1

公开(公告)日：1994-10-13

申请号：PCT/US1994003577

申请日：1994-04-01

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MOORE, Patrick, Q.

IPC: B04B05/04

CPC classification number: B01L3/5021 ,  B04B5/0414

Abstract: The present invention is directed to a floating cap or spacer (18) and centrifuge tube assembly (16) which provide adequate resistance to the deforming forces exerted on it. The exterior wall of the tube stem (24) is provided with an annular ridge (34) and the support spacer (18) is provided with an annular groove (36) which mates with the ridge (34) on the tube stem (24) to form an interlocking coupling.

Abstract translation: 本发明涉及一种浮动帽或间隔件（18）和离心管组件（16），其提供对施加在其上的变形力的足够的抵抗力。 管杆（24）的外壁设置有环形脊（34），并且支撑间隔件（18）设置有与管杆（24）上的脊（34）配合的环形槽（36） 以形成联锁联轴器。

公开(公告)号：WO1994003630A2

公开(公告)日：1994-02-17

申请号：PCT/US1993007342

申请日：1993-08-04

Applicant: BECKMAN INSTRUMENTS, INC. ,  ADAMS, Craig, W. ,  DANIELS, David, W.

Inventor： BECKMAN INSTRUMENTS, INC.

IPC: C12Q0

CPC classification number: C12Q1/6844 ,  C12Q1/6858 ,  C12Q1/6862 ,  C12Q2533/107 ,  C12Q2533/101 ,  C12Q2525/186

Abstract: Methods and reagents for the detection and exponential amplification of target nucleic acid molecules are disclosed. The method generally employs a Primer Oligonucleotide which hybridizes in concert with a Blocker Oligonucleotide on a strand of the target molecule, and an End-Run Oligonucleotide which can hybridize to the Blocker Oligonucleotide.

Abstract translation: 公开了靶核酸分子的检测和指数扩增的方法和试剂。 该方法通常使用引物寡核苷酸，其与目标分子的链上的阻断剂寡核苷酸和可以与阻断剂寡核苷酸杂交的末端寡核苷酸一起杂交。

公开(公告)号：WO1994001213A1

公开(公告)日：1994-01-20

申请号：PCT/US1993006182

申请日：1993-06-29

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  COASSIN, Peter, J. ,  HANNA, Edward, G. ,  MC KINNEY, Carlton, B. ,  RAMPAL, Jang, B. ,  SASAKI, Glenn, C. ,  WUNDERLY, Stephen, W.

IPC: B01J19/00

CPC classification number: C07H21/00 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00292 ,  B01J2219/00335 ,  B01J2219/00373 ,  B01J2219/00454 ,  B01J2219/005 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00686 ,  B01J2219/00722 ,  B01L3/502 ,  C07K1/045 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14

Abstract: The present invention is directed to a synthesis reaction column which can be conveniently implemented in post synthesis procedures. The reaction column is configured to allow easy connection to a syringe and post synthesis reagent container without requiring connection adapters. In one embodiment, the reaction column (17') configured in the form of a cylindrical column containing the solid-phase support (42) and having a narrow bore tube (18) extending at one end. The tube functions as a piercing tip for accessing a septum sealed vial (60) containing post synthesis reagent. In another embodiment, the reaction column (17'') is configured in the form of a cylindrical barrel (70) enclosing the solid-phase support (74) and having a threaded mount (80) at one end. To complement the reaction column for post synthesis procedures, a vial (92) having a complementary threaded mount (94) at the opening is used for containing a reagent. This vial (92) is conveniently threaded onto the end of the barrel (70) without adapters. Consequently, post synthesis procedures can be implemented conveniently. Both embodiments may be conveniently connected to the reagent delivery system in an automated instrument for carrying out synthesis procedures, as well as post synthesis procedures.

Abstract translation: 本发明涉及可以在后合成方法中方便地实施的合成反应塔。 反应塔被配置为允许容易地连接到注射器和后合成试剂容器而不需要连接适配器。 在一个实施方案中，反应塔（17'）构造成包含固相载体（42）并且在一端延伸的窄孔管（18）的圆柱形柱形式。 管用作用于进入含有后合成试剂的隔膜密封小瓶（60）的穿刺尖端。 在另一个实施例中，反应塔（17“）被构造为包围固相支撑件（74）并在一端具有螺纹安装件（80）的圆柱形筒体（70）的形式。 为了补充后合成过程的反应塔，在开口处具有互补螺纹安装件（94）的小瓶（92）用于容纳试剂。 该小瓶（92）方便地螺纹连接到筒体（70）的端部，而没有适配器。 因此，可以方便地实施后合成程序。 两个实施方案可以方便地连接到用于进行合成过程的自动化仪器中的试剂递送系统以及后合成程序。